KR102181103B1 - 생물학적 유체로부터 마이크로 rna 단리 - Google Patents

생물학적 유체로부터 마이크로 rna 단리 Download PDF

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KR102181103B1
KR102181103B1 KR1020167015578A KR20167015578A KR102181103B1 KR 102181103 B1 KR102181103 B1 KR 102181103B1 KR 1020167015578 A KR1020167015578 A KR 1020167015578A KR 20167015578 A KR20167015578 A KR 20167015578A KR 102181103 B1 KR102181103 B1 KR 102181103B1
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mirna
biological fluid
sodium
binding protein
surface active
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KR20160089398A (ko
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카롤 크레데르
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시그마-알드리치 컴퍼니., 엘엘씨
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    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21014—Microbial serine proteases (3.4.21.14)
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
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  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
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  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
KR1020167015578A 2013-11-27 2014-11-25 생물학적 유체로부터 마이크로 rna 단리 Active KR102181103B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361909834P 2013-11-27 2013-11-27
US61/909,834 2013-11-27
US201461985000P 2014-04-28 2014-04-28
US61/985,000 2014-04-28
PCT/US2014/067321 WO2015094609A1 (en) 2013-11-27 2014-11-25 Micro rna isolation from biological fluid

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KR20160089398A KR20160089398A (ko) 2016-07-27
KR102181103B1 true KR102181103B1 (ko) 2020-11-23

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US (2) US10131902B2 (enExample)
EP (2) EP3702361B1 (enExample)
JP (1) JP6644686B2 (enExample)
KR (1) KR102181103B1 (enExample)
CN (2) CN105934439B (enExample)
BR (1) BR112016011770B1 (enExample)
CA (1) CA2930704C (enExample)
ES (2) ES2778223T3 (enExample)
IL (1) IL245646B (enExample)
WO (1) WO2015094609A1 (enExample)

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CN105934439B (zh) 2013-11-27 2019-09-10 西格马-奥尔德里奇有限责任公司 从生物流体中分离微小rna
CN107385014B (zh) * 2017-06-13 2020-12-22 深圳大学 一种直接定量检测循环miRNA的RT-qPCR方法
KR102003594B1 (ko) 2017-08-08 2019-07-24 숙명여자대학교산학협력단 염증 반응 매개 신혈관생성 질환 진단용 조성물
CN111278844B (zh) * 2017-09-13 2023-12-19 贝克顿·迪金森公司 用于使用氧化铁颗粒提取核酸的方法和组合物
KR102811336B1 (ko) 2018-03-22 2025-05-21 아이덱스 래보러토리즈, 인코포레이티드 생물학적 샘플에서 박테리아를 측정하기 위한 방법
CN109490533B (zh) * 2018-12-29 2022-02-15 南京澳林生物科技有限公司 一种胃蛋白酶原ⅱ(pgⅱ)检测试剂盒及生产工艺
US20240312562A1 (en) * 2020-10-26 2024-09-19 Eclipse Bioinnovations, Inc. Methods and kits for enriching for polynucleotides
US11795500B2 (en) 2021-08-19 2023-10-24 Eclipse Bioinnovations, Inc. Methods for detecting RNA binding protein complexes
CN113980954A (zh) * 2021-11-11 2022-01-28 德州学院 一种病毒rna保护剂及其制备方法与应用

Citations (1)

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US20130197206A1 (en) * 2010-09-24 2013-08-01 Wako Pure Chemical Industries, Ltd. Method for acquisition of small rna

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MXPA06003886A (es) * 2003-10-08 2006-08-11 Ebioscience Reactivos de enlace de inmunoglobulina naturales y metodos de fabricacion y uso de los mismos.
JP5349838B2 (ja) * 2007-11-30 2013-11-20 和光純薬工業株式会社 ｓｍａｌｌＲＮＡの取得用担体、取得方法及び取得用試薬
WO2010014636A1 (en) * 2008-07-28 2010-02-04 Rockefeller University Methods for identifying rna segments bound by rna-binding proteins or ribonucleoprotein complexes
US8846314B2 (en) * 2009-03-03 2014-09-30 The Board Of Trustees Of The Leland Stanford Junior University Isotachophoretic focusing of nucleic acids
EP2678448A4 (en) * 2011-02-22 2014-10-01 Caris Life Sciences Luxembourg Holdings S A R L CIRCULATING BIOMARKERS
EP3208338B1 (en) * 2011-08-04 2019-04-10 Yeda Research and Development Co., Ltd. Micro-rna mir-15 and compositions comprising same for the treatment of corticotropin-releasing hormone- associated medical conditions
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WO2014082083A1 (en) * 2012-11-26 2014-05-30 Caris Science, Inc. Biomarker compositions and methods
US10435430B2 (en) * 2013-07-31 2019-10-08 Ionis Pharmaceuticals, Inc. Methods and compounds useful in conditions related to repeat expansion
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WO2016161375A2 (en) * 2015-04-03 2016-10-06 University Of Massachusetts Methods of using oligonucleotide-guided argonaute proteins

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EP3702361B1 (en) 2022-08-31
CA2930704C (en) 2023-02-21
EP3702361A1 (en) 2020-09-02
EP3074413B1 (en) 2020-01-22
US10711266B2 (en) 2020-07-14
CA2930704A1 (en) 2015-06-25
BR112016011770B1 (pt) 2022-10-25
ES2778223T3 (es) 2020-08-10
EP3074413A1 (en) 2016-10-05
CN105934439A (zh) 2016-09-07
JP2017501689A (ja) 2017-01-19
US10131902B2 (en) 2018-11-20
US20160376582A1 (en) 2016-12-29
JP6644686B2 (ja) 2020-02-12
IL245646B (en) 2018-06-28
ES2930344T3 (es) 2022-12-12
WO2015094609A1 (en) 2015-06-25
CN110511924A (zh) 2019-11-29
CN105934439B (zh) 2019-09-10
IL245646A0 (en) 2016-06-30
US20190144850A1 (en) 2019-05-16
BR112016011770A2 (enExample) 2017-08-08
CN110511924B (zh) 2023-11-03
EP3074413A4 (en) 2017-05-31
KR20160089398A (ko) 2016-07-27

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