ES2749700T3 - PCR extrema - Google Patents
PCR extrema Download PDFInfo
- Publication number
- ES2749700T3 ES2749700T3 ES13793389T ES13793389T ES2749700T3 ES 2749700 T3 ES2749700 T3 ES 2749700T3 ES 13793389 T ES13793389 T ES 13793389T ES 13793389 T ES13793389 T ES 13793389T ES 2749700 T3 ES2749700 T3 ES 2749700T3
- Authority
- ES
- Spain
- Prior art keywords
- polymerase
- pcr
- temperature
- concentration
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/02—Water baths; Sand baths; Air baths
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
- B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Apparatus For Radiation Diagnosis (AREA)
- Prostheses (AREA)
- Management, Administration, Business Operations System, And Electronic Commerce (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261651161P | 2012-05-24 | 2012-05-24 | |
US201361811145P | 2013-04-12 | 2013-04-12 | |
PCT/US2013/042473 WO2013177429A2 (fr) | 2012-05-24 | 2013-05-23 | Pcr extrême |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2749700T3 true ES2749700T3 (es) | 2020-03-23 |
Family
ID=49624526
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES13793389T Active ES2749700T3 (es) | 2012-05-24 | 2013-05-23 | PCR extrema |
ES19166256T Active ES2889348T3 (es) | 2012-05-24 | 2013-05-23 | Dispositivo para PCR extrema |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES19166256T Active ES2889348T3 (es) | 2012-05-24 | 2013-05-23 | Dispositivo para PCR extrema |
Country Status (12)
Country | Link |
---|---|
US (5) | US9932634B2 (fr) |
EP (3) | EP3957748A1 (fr) |
JP (3) | JP6316802B2 (fr) |
CN (2) | CN108251271A (fr) |
AU (1) | AU2013266246B2 (fr) |
CA (1) | CA2873991C (fr) |
DK (2) | DK3539666T3 (fr) |
ES (2) | ES2749700T3 (fr) |
PL (1) | PL3539666T3 (fr) |
PT (2) | PT2855692T (fr) |
SG (3) | SG10201800645WA (fr) |
WO (1) | WO2013177429A2 (fr) |
Families Citing this family (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9932634B2 (en) | 2012-05-24 | 2018-04-03 | University Of Utah Research Foundation | Methods for fast nucleic acid amplification |
WO2015069743A1 (fr) * | 2013-11-05 | 2015-05-14 | Biofire Diagnostics, Llc | Pcr par induction |
WO2016011280A1 (fr) * | 2014-07-16 | 2016-01-21 | Tangen Biosciences, Inc. | Procédés isothermes pour l'amplification d'échantillons d'acide nucléique |
EP3215260B1 (fr) | 2014-11-03 | 2020-01-15 | Tangen Biosciences Inc. | Appareil et procédé pour la capture et la rupture de cellules, de spores ou de virus |
CA2967011C (fr) * | 2014-11-07 | 2019-01-08 | Gna Biosolutions Gmbh | Procede pcr de superamplification |
TW201641687A (zh) * | 2014-12-31 | 2016-12-01 | 卡尤迪生物科技(北京)有限公司 | 用于進行化學反應的設備和方法 |
WO2016106717A1 (fr) * | 2014-12-31 | 2016-07-07 | Coyote Bioscience Co., Ltd. | Appareils et procédés permettant de réaliser des réactions chimiques |
ES2867949T3 (es) | 2015-06-02 | 2021-10-21 | Biofire Defense Llc | Muestra para secuenciar |
JP7126445B2 (ja) | 2015-11-02 | 2022-08-26 | バイオファイア・ダイアグノスティクス,リミテッド・ライアビリティ・カンパニー | 扱いにくい試料タイプのための試料調製 |
ES2916153T3 (es) | 2015-11-05 | 2022-06-28 | Univ Utah Res Found | PCR extrema para transcripción inversa |
CN109642198A (zh) * | 2016-02-10 | 2019-04-16 | 卡尤迪生物科技(北京)有限公司 | 用于分析核酸的方法和系统 |
AU2017223404B2 (en) | 2016-02-22 | 2022-09-22 | Biofire Defense, Llc | Devices and methods for rapid PCR |
JP6979029B2 (ja) | 2016-03-24 | 2021-12-08 | バイオファイア・ダイアグノスティクス,リミテッド・ライアビリティ・カンパニー | 定量的増幅方法 |
US11786906B2 (en) | 2016-04-15 | 2023-10-17 | Biofire Defense, Llc | Resistive heaters and anisotropic thermal transfer |
SG10201700260XA (en) * | 2016-06-10 | 2018-01-30 | Star Array Pte Ltd | Rapid thermal cycling for sample analyses and processing |
WO2017213586A1 (fr) * | 2016-06-10 | 2017-12-14 | Star Array Pte Ltd | Fluctuation thermique rapide destinée à des analyses et traitement d'échantillon |
WO2018022971A1 (fr) | 2016-07-28 | 2018-02-01 | Biofire Diagnostics, Llc. | Traitement d'acide nucléique indépendant |
JP2018023360A (ja) * | 2016-07-29 | 2018-02-15 | セイコーエプソン株式会社 | 核酸増幅反応方法、試薬、および試薬の使用方法 |
US10434515B2 (en) | 2016-10-03 | 2019-10-08 | University Of Utah Research Foundation | Thermal gradient plug flow microfluidic devices for extreme PCR |
US20190344280A1 (en) * | 2016-12-29 | 2019-11-14 | Biofire Defense, Llc | Fast pcr with molecular crowding |
WO2018204592A1 (fr) * | 2017-05-04 | 2018-11-08 | University Of Utah Research Foundation | Dispositif microfluidique pour pcr rapide |
WO2018217929A1 (fr) | 2017-05-24 | 2018-11-29 | Biofire Defense, Llc | Systèmes et procédés pour l'évacuation d'un réseau au point d'utilisation |
CN107502657A (zh) * | 2017-07-12 | 2017-12-22 | 浙江大学 | 一种极速pcr(聚合酶链式反应)扩增和终点检测方法 |
WO2019018099A1 (fr) | 2017-07-21 | 2019-01-24 | Biofire Diagnostics, Llc | Détection à l'aide de courbes de fusion concurrentes |
CN107930716B (zh) * | 2017-12-31 | 2023-05-09 | 江苏科泰检测技术服务有限公司 | 检测室用自动旋转式梯度恒温水浴锅及其使用方法 |
CN108251501A (zh) * | 2018-02-08 | 2018-07-06 | 中国农业大学 | 一种转基因作物双重基因的可视化定量筛查新方法 |
US20210180112A1 (en) | 2018-04-20 | 2021-06-17 | Biofire Diagnostics, Llc | Methods for normalization and quantification of sequencing data |
US20210123089A9 (en) | 2018-06-07 | 2021-04-29 | Biofire Diagnostics, Llc | Rapid thermocycling methods |
KR102116118B1 (ko) * | 2018-07-18 | 2020-05-27 | 주식회사 케이티앤지 | 에어로졸 생성장치의 히터의 온도를 구간별로 제어하는 방법 및 그 방법을 구현하기 위한 에어로졸 생성장치 |
GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
CN108752418B (zh) * | 2018-08-08 | 2024-01-30 | 湖南科技学院 | 一种多通道多肽合成反应装置及其操作方法 |
CN110114475B (zh) * | 2018-12-20 | 2021-06-18 | 广州市华南医学研究中心 | 一种极速核酸扩增的方法及其设备和应用 |
CN113727841B (zh) | 2018-12-21 | 2024-05-14 | 拜奥法尔诊断有限责任公司 | 用于反应容器的原位密封的设备、方法和系统 |
WO2020150442A1 (fr) * | 2019-01-17 | 2020-07-23 | Northwestern University | Réaction en chaîne par polymérase quantitative à transcription inverse rapide |
CN110387325A (zh) * | 2019-08-13 | 2019-10-29 | 广州市华南医学研究中心 | 一种极速pcr反应检测装置及检测方法 |
CN113493823A (zh) * | 2020-04-07 | 2021-10-12 | 中国农业大学 | 一种基于超速pcr与功能核酸的可视化检测方法 |
CN111974480A (zh) * | 2020-09-02 | 2020-11-24 | 杭州潘达科技有限公司 | 一种稳定效果好的具有多隔层的恒温水浴锅 |
CN113967487B (zh) * | 2021-10-11 | 2022-12-02 | 华中科技大学 | 一种喷嘴、液滴光热操控系统及其应用 |
GB202115637D0 (en) | 2021-11-01 | 2021-12-15 | Anglia Ruskin Univ Higher Education Corporation | Nucleic acid amplification method |
CN114214465A (zh) * | 2022-01-26 | 2022-03-22 | 山东仕达思生物产业有限公司 | 一种缩短新型冠状病毒检测时间的引物、探针、试剂盒及检测方法 |
WO2024110392A1 (fr) | 2022-11-22 | 2024-05-30 | bioMérieux | Nouveaux composés de liaison à l'acide nucléique et leurs utilisations |
Family Cites Families (26)
Publication number | Priority date | Publication date | Assignee | Title |
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CA2065719A1 (fr) * | 1991-04-30 | 1992-10-31 | John B. Findlay | Amplification d'acides nucleiques et methodes de detection faisant appel au cycle rapide de la reaction en chaine a la polymerase |
US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
WO1995004161A1 (fr) | 1993-07-29 | 1995-02-09 | Sergio Danilo Junho Pena | Methode d'identification de la sequence nucleotidique d'un segment purifie d'adn |
JP4335975B2 (ja) | 1995-06-07 | 2009-09-30 | ギリード・サイエンシズ・インコーポレーテッド | Dnaポリメラーゼに対する結合・阻害核酸リガンド |
AU7073096A (en) | 1995-09-19 | 1997-04-09 | University Of Massachusetts | Inhibited biological degradation of oligodeoxynucleotides |
US6210882B1 (en) | 1998-01-29 | 2001-04-03 | Mayo Foundation For Medical Education And Reseach | Rapid thermocycling for sample analysis |
US6780588B2 (en) * | 2001-05-07 | 2004-08-24 | Applera Corporation | Methods for the reduction of stutter in microsatellite amplification |
KR20030073875A (ko) | 2002-03-13 | 2003-09-19 | 주식회사 하이닉스반도체 | 반도체소자의 소자분리패턴 형성방법 |
US20040241655A1 (en) * | 2003-05-29 | 2004-12-02 | Yuchi Hwang | Conditional touchdown multiplex polymerase chain reaction |
US8232091B2 (en) * | 2006-05-17 | 2012-07-31 | California Institute Of Technology | Thermal cycling system |
KR20080029233A (ko) | 2006-09-28 | 2008-04-03 | 삼성전자주식회사 | 핵산 증폭 및 혼성화를 단일 고체 지지체에서 수행하는방법 및 장치 |
JP5191041B2 (ja) | 2007-04-05 | 2013-04-24 | エフ.ホフマン−ラ ロシュ アーゲー | 急速ワンステップrt−pcr |
WO2009105499A1 (fr) * | 2008-02-20 | 2009-08-27 | Termaat Joel R | Thermocycleur et récipient à échantillons pour l'amplification rapide de l'adn |
CA2658520C (fr) | 2008-03-19 | 2016-11-08 | F. Hoffmann-La Roche Ag | Amplification d'acide nucleique en presence d'oligonucleotides randomises modifies |
AU2009242546B2 (en) | 2008-04-30 | 2015-01-22 | Integrated Dna Technologies, Inc. | RNase-H-based assays utilizing modified RNA monomers |
US8349563B2 (en) | 2008-11-18 | 2013-01-08 | Life Technologies Corporation | Sequence amplification with target primers |
CN101407850B (zh) * | 2008-11-20 | 2011-01-05 | 江苏出入境检验检疫局动植物与食品检测中心 | 猴痘病毒的pcr快速检测试剂盒及检测方法 |
EP2406614A1 (fr) * | 2009-03-10 | 2012-01-18 | Agency For Science, Technology And Research | Appareil pour le traitement d'un échantillon biologique et/ou chimique |
EP2539472B1 (fr) * | 2010-02-26 | 2015-05-20 | Life Technologies Corporation | Pcr rapide pour le génotypage str |
US9005895B2 (en) | 2010-06-21 | 2015-04-14 | Life Technologies Corporation | Compositions, methods and kits for nucleic acid synthesis and amplification |
JP2012118055A (ja) * | 2010-11-12 | 2012-06-21 | Sony Corp | 反応処理装置及び反応処理方法 |
EP2651560B1 (fr) * | 2010-12-17 | 2020-03-18 | BJS IP Limited | Procédés et systèmes pour chauffage de pcr rapide |
WO2013132645A1 (fr) * | 2012-03-09 | 2013-09-12 | 独立行政法人産業技術総合研究所 | Procédé d'amplification d'acide nucléique |
US9932634B2 (en) | 2012-05-24 | 2018-04-03 | University Of Utah Research Foundation | Methods for fast nucleic acid amplification |
US9353409B2 (en) | 2013-01-15 | 2016-05-31 | Jun Euihum Lee | Compositions and methods for RT-PCR |
WO2015069743A1 (fr) | 2013-11-05 | 2015-05-14 | Biofire Diagnostics, Llc | Pcr par induction |
-
2013
- 2013-05-23 US US14/403,369 patent/US9932634B2/en active Active
- 2013-05-23 DK DK19166256.8T patent/DK3539666T3/da active
- 2013-05-23 ES ES13793389T patent/ES2749700T3/es active Active
- 2013-05-23 CA CA2873991A patent/CA2873991C/fr active Active
- 2013-05-23 EP EP21184359.4A patent/EP3957748A1/fr active Pending
- 2013-05-23 CN CN201810059052.4A patent/CN108251271A/zh active Pending
- 2013-05-23 EP EP19166256.8A patent/EP3539666B1/fr active Active
- 2013-05-23 PT PT137933891T patent/PT2855692T/pt unknown
- 2013-05-23 JP JP2015514196A patent/JP6316802B2/ja active Active
- 2013-05-23 SG SG10201800645WA patent/SG10201800645WA/en unknown
- 2013-05-23 PL PL19166256T patent/PL3539666T3/pl unknown
- 2013-05-23 SG SG11201407427WA patent/SG11201407427WA/en unknown
- 2013-05-23 ES ES19166256T patent/ES2889348T3/es active Active
- 2013-05-23 EP EP13793389.1A patent/EP2855692B1/fr active Active
- 2013-05-23 AU AU2013266246A patent/AU2013266246B2/en active Active
- 2013-05-23 PT PT191662568T patent/PT3539666T/pt unknown
- 2013-05-23 WO PCT/US2013/042473 patent/WO2013177429A2/fr active Application Filing
- 2013-05-23 DK DK13793389.1T patent/DK2855692T3/da active
- 2013-05-23 CN CN201380039367.8A patent/CN104662160B/zh active Active
- 2013-05-23 SG SG10201810110RA patent/SG10201810110RA/en unknown
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2018
- 2018-02-07 US US15/890,843 patent/US10144960B2/en active Active
- 2018-03-27 JP JP2018060111A patent/JP6731012B2/ja active Active
- 2018-11-02 US US16/179,383 patent/US11021744B2/en active Active
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2020
- 2020-04-07 JP JP2020069158A patent/JP7032818B2/ja active Active
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2021
- 2021-04-29 US US17/244,000 patent/US11834708B2/en active Active
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2023
- 2023-10-25 US US18/494,072 patent/US20240068021A1/en active Pending
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