ES2749700T3 - PCR extrema - Google Patents

PCR extrema Download PDF

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Publication number
ES2749700T3
ES2749700T3 ES13793389T ES13793389T ES2749700T3 ES 2749700 T3 ES2749700 T3 ES 2749700T3 ES 13793389 T ES13793389 T ES 13793389T ES 13793389 T ES13793389 T ES 13793389T ES 2749700 T3 ES2749700 T3 ES 2749700T3
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ES
Spain
Prior art keywords
polymerase
pcr
temperature
concentration
primer
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ES13793389T
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English (en)
Spanish (es)
Inventor
Carl Wittwer
Jared Farrar
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University of Utah Research Foundation UURF
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University of Utah Research Foundation UURF
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/02—Water baths; Sand baths; Air baths
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1894—Cooling means; Cryo cooling
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Apparatus For Radiation Diagnosis (AREA)
  • Prostheses (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
ES13793389T 2012-05-24 2013-05-23 PCR extrema Active ES2749700T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261651161P 2012-05-24 2012-05-24
US201361811145P 2013-04-12 2013-04-12
PCT/US2013/042473 WO2013177429A2 (fr) 2012-05-24 2013-05-23 Pcr extrême

Publications (1)

Publication Number Publication Date
ES2749700T3 true ES2749700T3 (es) 2020-03-23

Family

ID=49624526

Family Applications (2)

Application Number Title Priority Date Filing Date
ES13793389T Active ES2749700T3 (es) 2012-05-24 2013-05-23 PCR extrema
ES19166256T Active ES2889348T3 (es) 2012-05-24 2013-05-23 Dispositivo para PCR extrema

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES19166256T Active ES2889348T3 (es) 2012-05-24 2013-05-23 Dispositivo para PCR extrema

Country Status (12)

Country Link
US (5) US9932634B2 (fr)
EP (3) EP3957748A1 (fr)
JP (3) JP6316802B2 (fr)
CN (2) CN108251271A (fr)
AU (1) AU2013266246B2 (fr)
CA (1) CA2873991C (fr)
DK (2) DK3539666T3 (fr)
ES (2) ES2749700T3 (fr)
PL (1) PL3539666T3 (fr)
PT (2) PT2855692T (fr)
SG (3) SG10201800645WA (fr)
WO (1) WO2013177429A2 (fr)

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EP3215260B1 (fr) 2014-11-03 2020-01-15 Tangen Biosciences Inc. Appareil et procédé pour la capture et la rupture de cellules, de spores ou de virus
CA2967011C (fr) * 2014-11-07 2019-01-08 Gna Biosolutions Gmbh Procede pcr de superamplification
TW201641687A (zh) * 2014-12-31 2016-12-01 卡尤迪生物科技（北京）有限公司 用于進行化學反應的設備和方法
WO2016106717A1 (fr) * 2014-12-31 2016-07-07 Coyote Bioscience Co., Ltd. Appareils et procédés permettant de réaliser des réactions chimiques
ES2867949T3 (es) 2015-06-02 2021-10-21 Biofire Defense Llc Muestra para secuenciar
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ES2916153T3 (es) 2015-11-05 2022-06-28 Univ Utah Res Found PCR extrema para transcripción inversa
CN109642198A (zh) * 2016-02-10 2019-04-16 卡尤迪生物科技（北京）有限公司 用于分析核酸的方法和系统
AU2017223404B2 (en) 2016-02-22 2022-09-22 Biofire Defense, Llc Devices and methods for rapid PCR
JP6979029B2 (ja) 2016-03-24 2021-12-08 バイオファイア・ダイアグノスティクス，リミテッド・ライアビリティ・カンパニー 定量的増幅方法
US11786906B2 (en) 2016-04-15 2023-10-17 Biofire Defense, Llc Resistive heaters and anisotropic thermal transfer
SG10201700260XA (en) * 2016-06-10 2018-01-30 Star Array Pte Ltd Rapid thermal cycling for sample analyses and processing
WO2017213586A1 (fr) * 2016-06-10 2017-12-14 Star Array Pte Ltd Fluctuation thermique rapide destinée à des analyses et traitement d'échantillon
WO2018022971A1 (fr) 2016-07-28 2018-02-01 Biofire Diagnostics, Llc. Traitement d'acide nucléique indépendant
JP2018023360A (ja) * 2016-07-29 2018-02-15 セイコーエプソン株式会社 核酸増幅反応方法、試薬、および試薬の使用方法
US10434515B2 (en) 2016-10-03 2019-10-08 University Of Utah Research Foundation Thermal gradient plug flow microfluidic devices for extreme PCR
US20190344280A1 (en) * 2016-12-29 2019-11-14 Biofire Defense, Llc Fast pcr with molecular crowding
WO2018204592A1 (fr) * 2017-05-04 2018-11-08 University Of Utah Research Foundation Dispositif microfluidique pour pcr rapide
WO2018217929A1 (fr) 2017-05-24 2018-11-29 Biofire Defense, Llc Systèmes et procédés pour l'évacuation d'un réseau au point d'utilisation
CN107502657A (zh) * 2017-07-12 2017-12-22 浙江大学 一种极速pcr（聚合酶链式反应）扩增和终点检测方法
WO2019018099A1 (fr) 2017-07-21 2019-01-24 Biofire Diagnostics, Llc Détection à l'aide de courbes de fusion concurrentes
CN107930716B (zh) * 2017-12-31 2023-05-09 江苏科泰检测技术服务有限公司 检测室用自动旋转式梯度恒温水浴锅及其使用方法
CN108251501A (zh) * 2018-02-08 2018-07-06 中国农业大学 一种转基因作物双重基因的可视化定量筛查新方法
US20210180112A1 (en) 2018-04-20 2021-06-17 Biofire Diagnostics, Llc Methods for normalization and quantification of sequencing data
US20210123089A9 (en) 2018-06-07 2021-04-29 Biofire Diagnostics, Llc Rapid thermocycling methods
KR102116118B1 (ko) * 2018-07-18 2020-05-27 주식회사 케이티앤지 에어로졸 생성장치의 히터의 온도를 구간별로 제어하는 방법 및 그 방법을 구현하기 위한 에어로졸 생성장치
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN108752418B (zh) * 2018-08-08 2024-01-30 湖南科技学院 一种多通道多肽合成反应装置及其操作方法
CN110114475B (zh) * 2018-12-20 2021-06-18 广州市华南医学研究中心 一种极速核酸扩增的方法及其设备和应用
CN113727841B (zh) 2018-12-21 2024-05-14 拜奥法尔诊断有限责任公司 用于反应容器的原位密封的设备、方法和系统
WO2020150442A1 (fr) * 2019-01-17 2020-07-23 Northwestern University Réaction en chaîne par polymérase quantitative à transcription inverse rapide
CN110387325A (zh) * 2019-08-13 2019-10-29 广州市华南医学研究中心 一种极速pcr反应检测装置及检测方法
CN113493823A (zh) * 2020-04-07 2021-10-12 中国农业大学 一种基于超速pcr与功能核酸的可视化检测方法
CN111974480A (zh) * 2020-09-02 2020-11-24 杭州潘达科技有限公司 一种稳定效果好的具有多隔层的恒温水浴锅
CN113967487B (zh) * 2021-10-11 2022-12-02 华中科技大学 一种喷嘴、液滴光热操控系统及其应用
GB202115637D0 (en) 2021-11-01 2021-12-15 Anglia Ruskin Univ Higher Education Corporation Nucleic acid amplification method
CN114214465A (zh) * 2022-01-26 2022-03-22 山东仕达思生物产业有限公司 一种缩短新型冠状病毒检测时间的引物、探针、试剂盒及检测方法
WO2024110392A1 (fr) 2022-11-22 2024-05-30 bioMérieux Nouveaux composés de liaison à l'acide nucléique et leurs utilisations

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Also Published As

Publication number Publication date
CN104662160B (zh) 2018-02-23
EP2855692A2 (fr) 2015-04-08
US20240068021A1 (en) 2024-02-29
DK3539666T3 (da) 2021-09-13
SG11201407427WA (en) 2014-12-30
JP2018117632A (ja) 2018-08-02
EP3539666A1 (fr) 2019-09-18
WO2013177429A4 (fr) 2014-05-01
JP6316802B2 (ja) 2018-04-25
JP2020115878A (ja) 2020-08-06
US11021744B2 (en) 2021-06-01
CA2873991A1 (fr) 2013-11-28
US20210254132A1 (en) 2021-08-19
AU2013266246A1 (en) 2014-12-18
US20150118715A1 (en) 2015-04-30
PL3539666T3 (pl) 2022-01-31
PT2855692T (pt) 2019-10-25
EP2855692B1 (fr) 2019-07-24
CN104662160A (zh) 2015-05-27
JP7032818B2 (ja) 2022-03-09
WO2013177429A3 (fr) 2014-03-13
EP3539666B1 (fr) 2021-08-11
WO2013177429A2 (fr) 2013-11-28
US10144960B2 (en) 2018-12-04
US20180179581A1 (en) 2018-06-28
EP2855692A4 (fr) 2016-08-17
JP2015520614A (ja) 2015-07-23
CN108251271A (zh) 2018-07-06
EP3957748A1 (fr) 2022-02-23
DK2855692T3 (da) 2019-10-14
JP6731012B2 (ja) 2020-07-29
AU2013266246B2 (en) 2017-02-02
CA2873991C (fr) 2020-08-25
SG10201810110RA (en) 2018-12-28
PT3539666T (pt) 2021-09-23
SG10201800645WA (en) 2018-02-27
ES2889348T3 (es) 2022-01-12
US20190055591A1 (en) 2019-02-21
US9932634B2 (en) 2018-04-03
US11834708B2 (en) 2023-12-05

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