ES2747967T3 - Procedimientos y composiciones para generar progenitores pancreáticos y células beta funcionales a partir de hPSC - Google Patents

Procedimientos y composiciones para generar progenitores pancreáticos y células beta funcionales a partir de hPSC Download PDF

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ES2747967T3
ES2747967T3 ES13784648T ES13784648T ES2747967T3 ES 2747967 T3 ES2747967 T3 ES 2747967T3 ES 13784648 T ES13784648 T ES 13784648T ES 13784648 T ES13784648 T ES 13784648T ES 2747967 T3 ES2747967 T3 ES 2747967T3
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cells
nkx6
egf
population
cell
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Gordon Keller
Maria Cristina Nostro
Audrey Holtzinger
Farida Sarangi
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University Health Network
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Diabetes (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Endocrinology (AREA)
  • Immunology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Emergency Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES13784648T 2012-04-30 2013-04-30 Procedimientos y composiciones para generar progenitores pancreáticos y células beta funcionales a partir de hPSC Active ES2747967T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261640366P 2012-04-30 2012-04-30
PCT/CA2013/000432 WO2013163739A1 (en) 2012-04-30 2013-04-30 METHODS AND COMPOSITIONS FOR GENERATING PANCREATIC PROGENITORS AND FUNCTIONAL BETA CELLS FROM hPSCs

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ES2747967T3 true ES2747967T3 (es) 2020-03-12

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US (1) US20150104430A1 (enExample)
EP (2) EP3594323B1 (enExample)
JP (2) JP6397397B2 (enExample)
CN (2) CN108004199B (enExample)
AU (1) AU2013255031C1 (enExample)
CA (1) CA2871795C (enExample)
CY (1) CY1122058T1 (enExample)
DK (1) DK2844739T3 (enExample)
ES (1) ES2747967T3 (enExample)
HR (1) HRP20191677T1 (enExample)
HU (1) HUE046271T2 (enExample)
IN (1) IN2014DN10053A (enExample)
LT (1) LT2844739T (enExample)
PL (1) PL2844739T3 (enExample)
PT (1) PT2844739T (enExample)
SI (1) SI2844739T1 (enExample)
WO (1) WO2013163739A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014165663A1 (en) * 2013-04-03 2014-10-09 Cellular Dynamics International, Inc. Methods and compositions for culturing endoderm progenitor cells in suspension
CN106536718B (zh) * 2014-05-21 2021-04-27 国立大学法人京都大学 胰芽细胞的制造方法及含有胰芽细胞的胰疾病治疗剂
PT3285572T (pt) * 2015-04-24 2021-03-04 Univ Copenhagen Método para a produção de células produtoras de insulina
ES2840076T3 (es) 2015-04-24 2021-07-06 Univ Copenhagen Aislamiento de células progenitoras pancreáticas bona fide
US11332716B2 (en) 2015-07-27 2022-05-17 The Regents Of The University Of California Methods and compositions for producing pancreatic beta cells
CN106554936B (zh) * 2015-09-30 2020-05-05 海门雨霖细胞科技有限责任公司 诱导人干细胞向肝细胞定向分化的新方法
EP3420073B1 (en) * 2016-02-24 2023-11-08 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
MA45329A (fr) 2016-04-08 2019-02-13 Univ California Production de cellules bêta matures pleinement fonctionnelles à partir de progénitrices pancréatiques humaines
JP7065517B2 (ja) * 2016-07-26 2022-05-12 国立大学法人京都大学 Ｐｔｆ１ａ陽性細胞の製造方法
EP3591040A4 (en) * 2017-03-03 2020-11-11 Kyoto University METHOD OF PRODUCTION OF PANCREATIC PROGENITOR CELLS
JP2020527956A (ja) * 2017-07-21 2020-09-17 センマ・セラピューティクス・インコーポレーテッド 幹細胞由来の膵臓ベータ細胞の再凝集
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
WO2019099725A1 (en) 2017-11-15 2019-05-23 Semma Therapeutics, Inc. Islet cell manufacturing compositions and methods of use
US11530211B2 (en) 2018-01-18 2022-12-20 Daiichi Sankyo Company, Limited Dihydroindolizinone derivative
WO2019169351A1 (en) 2018-03-02 2019-09-06 Semma Therapeutics, Inc. Methods of enhancing stem cell differentiation into beta cells
WO2019227198A1 (en) 2018-05-31 2019-12-05 University Health Network (Uhn) Methods and compositions comprising tankyrase inhibitors for generating insulin producing cells
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
AU2018439389A1 (en) 2018-08-30 2021-02-18 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
CN111875674B (zh) * 2018-09-03 2021-12-10 内蒙古创润生物科技有限公司 表皮干细胞向胰腺细胞分化的改进方法
EP3868870B1 (en) * 2018-10-15 2025-12-03 Evia Life Sciences Inc. Method for producing stem/precursor cells, by using low molecular weight compound, from cells derived from endodermal tissue or organ
JP7385244B2 (ja) * 2019-06-27 2023-11-22 国立大学法人 東京大学 膵前駆細胞の分離方法
WO2021051256A1 (en) * 2019-09-17 2021-03-25 Center For Excellence In Molecular Cell Science, Chinese Academy Of Sciences Pancreatic pro-endocrine progenitor cells and use thereof
WO2022165383A1 (en) * 2021-02-01 2022-08-04 Cedars-Sinai Medical Center Generation of functional human ipsc-derived pancreatic islets in co-culture with isogenic ipsc-derived vascular endothelial cells
CN113234664B (zh) * 2021-05-11 2024-05-10 澳门大学 一种胰腺祖细胞的制备方法及其应用
CN114634904B (zh) * 2022-05-17 2022-09-13 天津外泌体科技有限公司 高纯度胰腺祖细胞的产生方法
CN118345030B (zh) * 2024-06-20 2024-08-16 羽铂精制生物技术(成都)有限公司 iPSC诱导分化成为胰腺祖细胞修复胰腺的方法及应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5994619A (en) 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
GB2399823B (en) * 2001-12-07 2006-02-15 Geron Corp Islet cells from primate pluripotent stem cells
US8187878B2 (en) 2004-08-13 2012-05-29 University Of Georgia Research Foundation, Inc. Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors
NZ571427A (en) 2006-03-02 2012-07-27 Viacyte Inc Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US7989204B2 (en) 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
US20080063628A1 (en) * 2006-09-11 2008-03-13 Davis Janet E Methods to promote cell differentiation
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US8338170B2 (en) * 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
GB201111244D0 (en) * 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
DK2516625T3 (da) * 2009-12-23 2024-09-09 Janssen Biotech Inc Differentiering af humane embryonale stamceller
WO2012070014A2 (en) * 2010-11-26 2012-05-31 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Identification of novel cell surface markers for pancreatic progenitor cells and definite endodermal cells

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DK2844739T3 (da) 2019-09-30
JP6759293B2 (ja) 2020-09-23
US20150104430A1 (en) 2015-04-16
CN108004199A (zh) 2018-05-08
CY1122058T1 (el) 2020-11-25
CN104411815B (zh) 2017-11-28
CN104411815A (zh) 2015-03-11
LT2844739T (lt) 2019-10-10
HRP20191677T1 (hr) 2019-12-13
HK1208047A1 (en) 2016-02-19
EP3594323A1 (en) 2020-01-15
WO2013163739A1 (en) 2013-11-07
EP2844739A4 (en) 2015-12-02
AU2013255031B2 (en) 2018-09-06
CN108004199B (zh) 2022-03-18
AU2013255031A1 (en) 2014-12-04
HUE046271T2 (hu) 2020-02-28
EP2844739B1 (en) 2019-06-19
CA2871795C (en) 2023-03-14
JP2018198620A (ja) 2018-12-20
HK1254968A1 (zh) 2019-08-02
CA2871795A1 (en) 2013-11-07
JP6397397B2 (ja) 2018-09-26
IN2014DN10053A (enExample) 2015-08-14
JP2015519048A (ja) 2015-07-09
PL2844739T3 (pl) 2019-12-31
AU2013255031C1 (en) 2018-12-06
EP3594323B1 (en) 2026-05-27
SI2844739T1 (sl) 2019-11-29
EP2844739A1 (en) 2015-03-11
PT2844739T (pt) 2019-10-01

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