CA2871795C - Methods and compositions for generating pancreatic progenitors and functional beta cells from hpscs - Google Patents

Methods and compositions for generating pancreatic progenitors and functional beta cells from hpscs Download PDF

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CA2871795C
CA2871795C CA2871795A CA2871795A CA2871795C CA 2871795 C CA2871795 C CA 2871795C CA 2871795 A CA2871795 A CA 2871795A CA 2871795 A CA2871795 A CA 2871795A CA 2871795 C CA2871795 C CA 2871795C
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cells
component
egf
conjugate
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CA2871795A1 (en
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Gordon Keller
Maria Cristina NOSTRO
Audrey HOLTZINGER
Farida SARANGI
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University Health Network
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Endocrinology (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Obesity (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2871795A 2012-04-30 2013-04-30 Methods and compositions for generating pancreatic progenitors and functional beta cells from hpscs Active CA2871795C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261640366P 2012-04-30 2012-04-30
US61/640,366 2012-04-30
PCT/CA2013/000432 WO2013163739A1 (en) 2012-04-30 2013-04-30 METHODS AND COMPOSITIONS FOR GENERATING PANCREATIC PROGENITORS AND FUNCTIONAL BETA CELLS FROM hPSCs

Publications (2)

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CA2871795A1 CA2871795A1 (en) 2013-11-07
CA2871795C true CA2871795C (en) 2023-03-14

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CA2871795A Active CA2871795C (en) 2012-04-30 2013-04-30 Methods and compositions for generating pancreatic progenitors and functional beta cells from hpscs

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US (1) US20150104430A1 (enExample)
EP (2) EP3594323A1 (enExample)
JP (2) JP6397397B2 (enExample)
CN (2) CN104411815B (enExample)
AU (1) AU2013255031C1 (enExample)
CA (1) CA2871795C (enExample)
CY (1) CY1122058T1 (enExample)
DK (1) DK2844739T3 (enExample)
ES (1) ES2747967T3 (enExample)
HR (1) HRP20191677T1 (enExample)
HU (1) HUE046271T2 (enExample)
IN (1) IN2014DN10053A (enExample)
LT (1) LT2844739T (enExample)
PL (1) PL2844739T3 (enExample)
PT (1) PT2844739T (enExample)
SI (1) SI2844739T1 (enExample)
WO (1) WO2013163739A1 (enExample)

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WO2014165663A1 (en) * 2013-04-03 2014-10-09 Cellular Dynamics International, Inc. Methods and compositions for culturing endoderm progenitor cells in suspension
US10472610B2 (en) 2014-05-21 2019-11-12 Kyoto University Method for generating pancreatic bud cells and therapeutic agent for pancreatic disease containing pancreatic bud cells
JP6954711B2 (ja) 2015-04-24 2021-10-27 ユニバーシティ オブ コペンハーゲン 真正膵臓前駆細胞の単離
DK3285572T3 (da) * 2015-04-24 2021-03-01 Univ Copenhagen Fremgangsmåde til fremstilling af insulinproducerende celler
WO2017019702A1 (en) * 2015-07-27 2017-02-02 The Regents Of The University Of California Methods and compositions for producing pancreatic beta cells
CN106554936B (zh) * 2015-09-30 2020-05-05 海门雨霖细胞科技有限责任公司 诱导人干细胞向肝细胞定向分化的新方法
MA45747A (fr) * 2016-02-24 2019-01-02 Novo Nordisk As Génération de cellules bêta fonctionnelles à partir de progéniteurs endocrines dérivés de cellules souhes pluripotentes humaines
ES3002713T3 (en) * 2016-04-08 2025-03-07 Univ California Production of fully functional mature beta cells from human pancreatic progenitors
JP7065517B2 (ja) * 2016-07-26 2022-05-12 国立大学法人京都大学 Ｐｔｆ１ａ陽性細胞の製造方法
EP3591040A4 (en) * 2017-03-03 2020-11-11 Kyoto University METHOD OF PRODUCTION OF PANCREATIC PROGENITOR CELLS
EP4488363A3 (en) * 2017-07-21 2025-03-26 Vertex Pharmaceuticals Incorporated Re-aggregation of stem cell-derived pancreatic beta cells
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
AU2018370029B2 (en) 2017-11-15 2024-11-07 Vertex Pharmaceuticals Incorporated Islet cell manufacturing compositions and methods of use
ES2958118T3 (es) 2018-01-18 2024-02-01 Daiichi Sankyo Co Ltd Derivado de dihidroindolizinona
CA3101021A1 (en) 2018-05-31 2019-12-05 University Health Network Methods and compositions comprising tankyrase inhibitors for generating insulin producing cells
WO2020033879A1 (en) 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Stem cell derived islet differentiation
AU2018439389A1 (en) 2018-08-30 2021-02-18 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
CN111875674B (zh) * 2018-09-03 2021-12-10 内蒙古创润生物科技有限公司 表皮干细胞向胰腺细胞分化的改进方法
DK3868870T3 (en) * 2018-10-15 2026-02-23 Evia Life Sciences Inc Method for producing stem/precursor cells, by using low molecular weight compound, from cells derived from endodermal tissue or organ
JP7385244B2 (ja) * 2019-06-27 2023-11-22 国立大学法人 東京大学 膵前駆細胞の分離方法
WO2021051256A1 (en) * 2019-09-17 2021-03-25 Center For Excellence In Molecular Cell Science, Chinese Academy Of Sciences Pancreatic pro-endocrine progenitor cells and use thereof
US20240093154A1 (en) * 2021-02-01 2024-03-21 Cedars-Sinai Medical Center Generation of functional human ipsc-derived pancreatic islets in co-culture with isogenic ipsc-derived vascular endothelial cells
CN113234664B (zh) * 2021-05-11 2024-05-10 澳门大学 一种胰腺祖细胞的制备方法及其应用
CN114634904B (zh) * 2022-05-17 2022-09-13 天津外泌体科技有限公司 高纯度胰腺祖细胞的产生方法
CN118345030B (zh) * 2024-06-20 2024-08-16 羽铂精制生物技术(成都)有限公司 iPSC诱导分化成为胰腺祖细胞修复胰腺的方法及应用

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Publication number Publication date
CN104411815A (zh) 2015-03-11
AU2013255031B2 (en) 2018-09-06
HRP20191677T1 (hr) 2019-12-13
HK1208047A1 (en) 2016-02-19
LT2844739T (lt) 2019-10-10
SI2844739T1 (sl) 2019-11-29
CN104411815B (zh) 2017-11-28
ES2747967T3 (es) 2020-03-12
HUE046271T2 (hu) 2020-02-28
US20150104430A1 (en) 2015-04-16
JP2015519048A (ja) 2015-07-09
JP6397397B2 (ja) 2018-09-26
CN108004199B (zh) 2022-03-18
CN108004199A (zh) 2018-05-08
HK1254968A1 (zh) 2019-08-02
EP2844739A4 (en) 2015-12-02
EP2844739B1 (en) 2019-06-19
EP3594323A1 (en) 2020-01-15
JP6759293B2 (ja) 2020-09-23
AU2013255031C1 (en) 2018-12-06
EP2844739A1 (en) 2015-03-11
JP2018198620A (ja) 2018-12-20
AU2013255031A1 (en) 2014-12-04
DK2844739T3 (da) 2019-09-30
WO2013163739A1 (en) 2013-11-07
PT2844739T (pt) 2019-10-01
CA2871795A1 (en) 2013-11-07
CY1122058T1 (el) 2020-11-25
PL2844739T3 (pl) 2019-12-31
IN2014DN10053A (enExample) 2015-08-14

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