ES2656856T3 - Amplificación de ácido nucleico - Google Patents
Amplificación de ácido nucleico Download PDFInfo
- Publication number
- ES2656856T3 ES2656856T3 ES12737335.5T ES12737335T ES2656856T3 ES 2656856 T3 ES2656856 T3 ES 2656856T3 ES 12737335 T ES12737335 T ES 12737335T ES 2656856 T3 ES2656856 T3 ES 2656856T3
- Authority
- ES
- Spain
- Prior art keywords
- mixture
- amplification
- nucleic acid
- concentration
- less
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 39
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 38
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 38
- 230000003321 amplification Effects 0.000 title claims description 76
- 238000003199 nucleic acid amplification method Methods 0.000 title claims description 76
- 239000000203 mixture Substances 0.000 claims abstract description 50
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000011901 isothermal amplification Methods 0.000 claims abstract description 13
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims abstract description 7
- 150000003868 ammonium compounds Chemical class 0.000 claims abstract description 5
- 229960000789 guanidine hydrochloride Drugs 0.000 claims abstract 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims abstract 2
- 159000000003 magnesium salts Chemical class 0.000 claims abstract 2
- 239000011541 reaction mixture Substances 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims description 39
- 230000003139 buffering effect Effects 0.000 claims description 33
- 239000006172 buffering agent Substances 0.000 claims description 22
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- 102000004190 Enzymes Human genes 0.000 claims description 16
- 108090000790 Enzymes Proteins 0.000 claims description 16
- 239000002773 nucleotide Substances 0.000 claims description 15
- 125000003729 nucleotide group Chemical group 0.000 claims description 15
- 238000001514 detection method Methods 0.000 claims description 14
- 239000007793 ph indicator Substances 0.000 claims description 9
- 108700028369 Alleles Proteins 0.000 claims description 7
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 7
- 235000019270 ammonium chloride Nutrition 0.000 claims description 7
- 230000035484 reaction time Effects 0.000 claims description 7
- 150000003467 sulfuric acid derivatives Chemical class 0.000 claims description 5
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 claims description 4
- 230000000295 complement effect Effects 0.000 claims description 4
- 238000012544 monitoring process Methods 0.000 claims description 4
- 238000011002 quantification Methods 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 2
- 239000008151 electrolyte solution Substances 0.000 claims 1
- 150000004767 nitrides Chemical class 0.000 claims 1
- 239000000872 buffer Substances 0.000 abstract description 17
- 239000003153 chemical reaction reagent Substances 0.000 description 39
- 108020004414 DNA Proteins 0.000 description 28
- 238000006243 chemical reaction Methods 0.000 description 27
- 239000012530 fluid Substances 0.000 description 20
- 239000000523 sample Substances 0.000 description 19
- 238000007397 LAMP assay Methods 0.000 description 15
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 14
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 12
- 239000007983 Tris buffer Substances 0.000 description 12
- 238000006073 displacement reaction Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 8
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 6
- 235000019341 magnesium sulphate Nutrition 0.000 description 6
- -1 MgSO4 Chemical compound 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 4
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 230000005669 field effect Effects 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- RZQXOGQSPBYUKH-UHFFFAOYSA-N 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCC(CO)(CO)NCC(O)CS(O)(=O)=O RZQXOGQSPBYUKH-UHFFFAOYSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 239000007995 HEPES buffer Substances 0.000 description 3
- 239000007993 MOPS buffer Substances 0.000 description 3
- 108091092878 Microsatellite Proteins 0.000 description 3
- JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 229960003237 betaine Drugs 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 238000010348 incorporation Methods 0.000 description 3
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 2
- 108700016232 Arg(2)-Sar(4)- dermorphin (1-4) Proteins 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 101100532034 Drosophila melanogaster RTase gene Proteins 0.000 description 2
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 2
- 239000007990 PIPES buffer Substances 0.000 description 2
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
- 239000007994 TES buffer Substances 0.000 description 2
- 208000034953 Twin anemia-polycythemia sequence Diseases 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- OGGXGZAMXPVRFZ-UHFFFAOYSA-M dimethylarsinate Chemical compound C[As](C)([O-])=O OGGXGZAMXPVRFZ-UHFFFAOYSA-M 0.000 description 2
- OGGXGZAMXPVRFZ-UHFFFAOYSA-N dimethylarsinic acid Chemical compound C[As](C)(O)=O OGGXGZAMXPVRFZ-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 150000003839 salts Chemical group 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- BJFILYACUCTLKB-UHFFFAOYSA-N 2-chloroguanidine Chemical compound NC(N)=NCl BJFILYACUCTLKB-UHFFFAOYSA-N 0.000 description 1
- 238000010953 Ames test Methods 0.000 description 1
- 231100000039 Ames test Toxicity 0.000 description 1
- 241000713838 Avian myeloblastosis virus Species 0.000 description 1
- 108010043461 Deep Vent DNA polymerase Proteins 0.000 description 1
- 101000794020 Homo sapiens Bromodomain-containing protein 8 Proteins 0.000 description 1
- 101001006782 Homo sapiens Kinesin-associated protein 3 Proteins 0.000 description 1
- 101000615355 Homo sapiens Small acidic protein Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 description 1
- WGKGADVPRVLHHZ-ZHRMCQFGSA-N N-[(1R,2R,3S)-2-hydroxy-3-phenoxazin-10-ylcyclohexyl]-4-(trifluoromethoxy)benzenesulfonamide Chemical compound O[C@H]1[C@@H](CCC[C@@H]1N1C2=CC=CC=C2OC2=C1C=CC=C2)NS(=O)(=O)C1=CC=C(OC(F)(F)F)C=C1 WGKGADVPRVLHHZ-ZHRMCQFGSA-N 0.000 description 1
- YNLCVAQJIKOXER-UHFFFAOYSA-N N-[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid Chemical compound OCC(CO)(CO)NCCCS(O)(=O)=O YNLCVAQJIKOXER-UHFFFAOYSA-N 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 229910052581 Si3N4 Inorganic materials 0.000 description 1
- 229910021607 Silver chloride Inorganic materials 0.000 description 1
- 102100021255 Small acidic protein Human genes 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 1
- 101000865057 Thermococcus litoralis DNA polymerase Proteins 0.000 description 1
- 239000007997 Tricine buffer Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical group 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229950004243 cacodylic acid Drugs 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 150000001793 charged compounds Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 description 1
- GTKRFUAGOKINCA-UHFFFAOYSA-M chlorosilver;silver Chemical compound [Ag].[Ag]Cl GTKRFUAGOKINCA-UHFFFAOYSA-M 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- CCIVGXIOQKPBKL-UHFFFAOYSA-N ethanesulfonic acid Chemical compound CCS(O)(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-N 0.000 description 1
- 230000003631 expected effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- VDEGQTCMQUFPFH-UHFFFAOYSA-N hydroxy-dimethyl-arsine Natural products C[As](C)O VDEGQTCMQUFPFH-UHFFFAOYSA-N 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000012125 lateral flow test Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 150000002681 magnesium compounds Chemical class 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- KXXXUIKPSVVSAW-UHFFFAOYSA-K pyranine Chemical compound [Na+].[Na+].[Na+].C1=C2C(O)=CC(S([O-])(=O)=O)=C(C=C3)C2=C2C3=C(S([O-])(=O)=O)C=C(S([O-])(=O)=O)C2=C1 KXXXUIKPSVVSAW-UHFFFAOYSA-K 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000005496 tempering Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1112140.7A GB201112140D0 (en) | 2011-07-14 | 2011-07-14 | Nucleic acid amplification |
| GB201112140 | 2011-07-14 | ||
| PCT/GB2012/051694 WO2013008042A1 (en) | 2011-07-14 | 2012-07-16 | Nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2656856T3 true ES2656856T3 (es) | 2018-02-28 |
Family
ID=44586619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12737335.5T Active ES2656856T3 (es) | 2011-07-14 | 2012-07-16 | Amplificación de ácido nucleico |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9469870B2 (https=) |
| EP (1) | EP2732050B1 (https=) |
| JP (1) | JP6139519B2 (https=) |
| CN (1) | CN103687962B (https=) |
| ES (1) | ES2656856T3 (https=) |
| GB (2) | GB201112140D0 (https=) |
| WO (1) | WO2013008042A1 (https=) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201112140D0 (en) | 2011-07-14 | 2011-08-31 | Dna Electronics | Nucleic acid amplification |
| WO2015033229A2 (en) * | 2013-09-04 | 2015-03-12 | Credo Biomedical Pte Ltd. | Assay test device, kit and method of using |
| JP6759103B2 (ja) | 2014-03-31 | 2020-09-23 | インポッシブル フーズ インコーポレイテッド | ひき肉レプリカ |
| CN103923998A (zh) * | 2014-04-23 | 2014-07-16 | 浙江大学 | 一种应用于核酸扩增实时电化学pH检测的反应体系和检测方法 |
| EP3594360B1 (en) | 2014-04-24 | 2021-06-23 | Lucira Health, Inc. | Colorimetric detection of nucleic acid amplification |
| US10249741B2 (en) * | 2014-05-13 | 2019-04-02 | Joseph T. Smith | System and method for ion-selective, field effect transistor on flexible substrate |
| AU2017232340B2 (en) | 2016-03-14 | 2022-04-28 | Pfizer Inc. | Systems and methods for performing biological assays |
| ES2927062T3 (es) | 2016-03-14 | 2022-11-02 | Lucira Health Inc | Dispositivos y procedimientos para modificar propiedades ópticas |
| JP6949864B2 (ja) | 2016-03-14 | 2021-10-13 | ルシラ ヘルス インコーポレイテッド | 生物学的アッセイ試料調製および送り出しのための装置および方法 |
| EP3429543B1 (en) | 2016-03-14 | 2024-11-20 | Pfizer Inc. | Selectively vented biological assay devices and associated methods |
| US11080848B2 (en) | 2017-04-06 | 2021-08-03 | Lucira Health, Inc. | Image-based disease diagnostics using a mobile device |
| CN113777011B (zh) | 2017-09-14 | 2024-11-19 | 辉瑞公司 | 具有电子读出的复用生物测定装置 |
| US10549275B2 (en) | 2017-09-14 | 2020-02-04 | Lucira Health, Inc. | Multiplexed biological assay device with electronic readout |
| US20200392562A1 (en) * | 2017-12-22 | 2020-12-17 | Thermo Fisher Scientific Baltics Uab | Polymerase chain reaction composition comprising amines |
| GB2570157A (en) * | 2018-01-15 | 2019-07-17 | Dnanudge Ltd | Molecule detection |
| JP2021521462A (ja) | 2018-03-29 | 2021-08-26 | ファウンデーション オブ イノベーティブ ニュー ダイアグノスティクス | 抗体または抗体の組み合わせおよび対象の尿試料中のマイコバクテリウム関連抗原の検出のためのその使用 |
| GB201809420D0 (en) * | 2018-06-08 | 2018-07-25 | Imperial Innovations Ltd | Devices and method for detecting an amplification event |
| US10467679B1 (en) | 2019-04-15 | 2019-11-05 | Dnanudge Limited | Product recommendation device and method |
| USD910200S1 (en) | 2018-12-21 | 2021-02-09 | Lucira Health, Inc. | Test tube |
| US10811140B2 (en) | 2019-03-19 | 2020-10-20 | Dnanudge Limited | Secure set-up of genetic related user account |
| US10699806B1 (en) | 2019-04-15 | 2020-06-30 | Dnanudge Limited | Monitoring system, wearable monitoring device and method |
| WO2021025455A1 (ko) * | 2019-08-06 | 2021-02-11 | 주식회사 지엠디바이오텍 | 일체형 분자 진단 장치 |
| AU2021249488A1 (en) * | 2020-04-03 | 2022-09-08 | Diagonal Bio Ab | A method of measuring the pH of a sample |
| EP3895798A1 (en) | 2020-04-17 | 2021-10-20 | Pall Corporation | Flow cell for use in a fluid management and/or processing system |
| USD953561S1 (en) | 2020-05-05 | 2022-05-31 | Lucira Health, Inc. | Diagnostic device with LED display |
| USD962470S1 (en) | 2020-06-03 | 2022-08-30 | Lucira Health, Inc. | Assay device with LCD display |
| US20220228205A1 (en) * | 2021-01-15 | 2022-07-21 | Purdue Research Foundation | Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets |
| US20220228226A1 (en) | 2021-01-15 | 2022-07-21 | Purdue Research Foundation | Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets |
| US12292407B2 (en) * | 2021-02-24 | 2025-05-06 | Robert Bosch Gmbh | Measuring ion strength using closed-loop electrochemical ph modulation |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE374833T1 (de) | 1998-11-09 | 2007-10-15 | Eiken Chemical | Verfahren zur synthese von nukleinsäuren |
| CN1254548C (zh) * | 2000-08-23 | 2006-05-03 | 宝生物工程株式会社 | 核酸扩增方法 |
| GB0105831D0 (en) | 2001-03-09 | 2001-04-25 | Toumaz Technology Ltd | Method for dna sequencing utilising enzyme linked field effect transistors |
| US8114591B2 (en) | 2001-03-09 | 2012-02-14 | Dna Electronics Ltd. | Sensing apparatus and method |
| US7534873B2 (en) | 2003-08-29 | 2009-05-19 | Applied Biosystems, Llc | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| AU2005323451B2 (en) | 2004-05-21 | 2010-11-18 | Qiagen Sciences Llc | Kits and processes for removing contaminants from nucleic acids in environmental and biological samples |
| EP3796002B1 (en) * | 2006-07-14 | 2023-11-22 | The Regents of The University of California | Cancer biomarkers and methods of use thereof |
| JP4918409B2 (ja) * | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| SG175559A1 (en) | 2006-09-25 | 2011-11-28 | Advanced Tech Materials | Compositions and methods for the removal of photoresist for a wafer rework application |
| ES2440572T3 (es) * | 2007-03-02 | 2014-01-29 | Dna Electronics Ltd | Aparato de detección para controlar la amplificación de ácido nucleico, usando un transistor de efecto de campo sensible a iones (ISFET) para detectar el pH |
| WO2009072156A1 (en) * | 2007-12-07 | 2009-06-11 | Emanuela Manna | Deodorizing and sanitizing compositions |
| US8470164B2 (en) | 2008-06-25 | 2013-06-25 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| WO2010037085A1 (en) * | 2008-09-29 | 2010-04-01 | The Board Of Trustees Of The University Of Illinois | Dna sequencing and amplification systems using nanoscale field effect sensor arrays |
| JP5572943B2 (ja) * | 2008-12-17 | 2014-08-20 | 株式会社島津製作所 | 血液検体からのｄｎａ増幅方法及びｄｎａ増幅キット |
| ES2351294B8 (es) * | 2009-07-02 | 2012-06-06 | Consejo Superior De Investigaciones Científicas (Csic) | Metodo para la replicación, amplificación, o secuenciación de un adn molde. |
| GB201112140D0 (en) | 2011-07-14 | 2011-08-31 | Dna Electronics | Nucleic acid amplification |
-
2011
- 2011-07-14 GB GBGB1112140.7A patent/GB201112140D0/en not_active Ceased
-
2012
- 2012-07-16 EP EP12737335.5A patent/EP2732050B1/en active Active
- 2012-07-16 JP JP2014519635A patent/JP6139519B2/ja active Active
- 2012-07-16 WO PCT/GB2012/051694 patent/WO2013008042A1/en not_active Ceased
- 2012-07-16 CN CN201280034733.6A patent/CN103687962B/zh active Active
- 2012-07-16 ES ES12737335.5T patent/ES2656856T3/es active Active
- 2012-07-16 GB GB1212637.1A patent/GB2494239B/en active Active
- 2012-07-16 US US14/232,817 patent/US9469870B2/en active Active
-
2016
- 2016-09-30 US US15/282,879 patent/US9840733B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| GB2494239B (en) | 2014-05-21 |
| US20170016058A1 (en) | 2017-01-19 |
| CN103687962B (zh) | 2015-12-09 |
| GB2494239A (en) | 2013-03-06 |
| CN103687962A (zh) | 2014-03-26 |
| GB201212637D0 (en) | 2012-08-29 |
| US20140170661A1 (en) | 2014-06-19 |
| JP2014520544A (ja) | 2014-08-25 |
| EP2732050B1 (en) | 2017-11-15 |
| WO2013008042A1 (en) | 2013-01-17 |
| US9840733B2 (en) | 2017-12-12 |
| GB201112140D0 (en) | 2011-08-31 |
| JP6139519B2 (ja) | 2017-05-31 |
| US9469870B2 (en) | 2016-10-18 |
| EP2732050A1 (en) | 2014-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2656856T3 (es) | Amplificación de ácido nucleico | |
| ES2668467T3 (es) | Métodos de amplificación LATE y secuenciación de un ácido nucleico | |
| ES2743050T5 (es) | Composiciones y procedimientos para cuantificar una secuencia de ácido nucleico en una muestra | |
| ES2893411T3 (es) | Procedimientos para realizar PCR multiplexada | |
| ES2523140T3 (es) | Aumento de la confianza en las identificaciones de alelos con el recuento molecular | |
| US10041111B2 (en) | Method for detecting and quantifying biomaterials by using activity of nucleic acid polymerase regulated by target material | |
| DE60108897D1 (de) | Methoden für externe kontrollen zur nukleinsäure amplifizierung | |
| HRP20200264T1 (hr) | Nukleinskokiselinska sonda s jednim fluorofornim biljegom vezanim na unutarnji citozin, namijenjena upotrebi kod izotermalne amplifikacije posredovane petljom | |
| ES2928304T3 (es) | Un método para medir el pH de una muestra | |
| US9845495B2 (en) | Method and kit for detecting target nucleic acid | |
| US10036011B2 (en) | Scavenger compounds for improved sequencing-by-synthesis | |
| DE60332775D1 (de) | Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen | |
| US20200362404A1 (en) | Electrochemical Detection Of Polymerase Reactions By Specific Metal-Phosphate Complex Formation | |
| ES2716094T3 (es) | Métodos para analizar la contaminación en la secuenciación del ADN | |
| ES2271436T3 (es) | Sondas de hibridacion fluorescente con poco ruido de fondo. | |
| US20180148781A1 (en) | Antioxidant Compounds For Cleave Formulations That Support Long Reads In Sequencing-By-Synthesis | |
| US10975423B2 (en) | Methods for true isothermal strand displacement amplification | |
| ES2886605T3 (es) | Procedimiento genérico para la estabilización de ARN específico | |
| US20220333167A1 (en) | Combined solution phase and solid phase dna amplification | |
| US20200149104A1 (en) | Additive To Improve Sequencing By Synthesis Performance | |
| ES2373496T3 (es) | Detección de la resistencia a triazol en aspergillus. | |
| US20170268042A1 (en) | Pcr method and pcr kit | |
| US10760116B2 (en) | Analysis kit, analyzer, and methods for analyzing template nucleic acid or target substance | |
| Ishizuka et al. | Clipping of Telomere from Human Chromosomes Using a Chemistry‐Based Artificial Restriction DNA Cutter | |
| WO2021080629A1 (en) | Methods for true isothermal strand displacement amplification |