CN103687962B - 核酸扩增 - Google Patents
核酸扩增 Download PDFInfo
- Publication number
- CN103687962B CN103687962B CN201280034733.6A CN201280034733A CN103687962B CN 103687962 B CN103687962 B CN 103687962B CN 201280034733 A CN201280034733 A CN 201280034733A CN 103687962 B CN103687962 B CN 103687962B
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- Prior art keywords
- mixture
- amplification
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- nucleic acid
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1112140.7 | 2011-07-14 | ||
| GBGB1112140.7A GB201112140D0 (en) | 2011-07-14 | 2011-07-14 | Nucleic acid amplification |
| PCT/GB2012/051694 WO2013008042A1 (en) | 2011-07-14 | 2012-07-16 | Nucleic acid amplification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103687962A CN103687962A (zh) | 2014-03-26 |
| CN103687962B true CN103687962B (zh) | 2015-12-09 |
Family
ID=44586619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280034733.6A Active CN103687962B (zh) | 2011-07-14 | 2012-07-16 | 核酸扩增 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9469870B2 (https=) |
| EP (1) | EP2732050B1 (https=) |
| JP (1) | JP6139519B2 (https=) |
| CN (1) | CN103687962B (https=) |
| ES (1) | ES2656856T3 (https=) |
| GB (2) | GB201112140D0 (https=) |
| WO (1) | WO2013008042A1 (https=) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201112140D0 (en) | 2011-07-14 | 2011-08-31 | Dna Electronics | Nucleic acid amplification |
| WO2015033229A2 (en) * | 2013-09-04 | 2015-03-12 | Credo Biomedical Pte Ltd. | Assay test device, kit and method of using |
| JP6759103B2 (ja) | 2014-03-31 | 2020-09-23 | インポッシブル フーズ インコーポレイテッド | ひき肉レプリカ |
| CN103923998A (zh) * | 2014-04-23 | 2014-07-16 | 浙江大学 | 一种应用于核酸扩增实时电化学pH检测的反应体系和检测方法 |
| EP3594360B1 (en) | 2014-04-24 | 2021-06-23 | Lucira Health, Inc. | Colorimetric detection of nucleic acid amplification |
| US10249741B2 (en) * | 2014-05-13 | 2019-04-02 | Joseph T. Smith | System and method for ion-selective, field effect transistor on flexible substrate |
| AU2017232340B2 (en) | 2016-03-14 | 2022-04-28 | Pfizer Inc. | Systems and methods for performing biological assays |
| ES2927062T3 (es) | 2016-03-14 | 2022-11-02 | Lucira Health Inc | Dispositivos y procedimientos para modificar propiedades ópticas |
| JP6949864B2 (ja) | 2016-03-14 | 2021-10-13 | ルシラ ヘルス インコーポレイテッド | 生物学的アッセイ試料調製および送り出しのための装置および方法 |
| EP3429543B1 (en) | 2016-03-14 | 2024-11-20 | Pfizer Inc. | Selectively vented biological assay devices and associated methods |
| US11080848B2 (en) | 2017-04-06 | 2021-08-03 | Lucira Health, Inc. | Image-based disease diagnostics using a mobile device |
| CN113777011B (zh) | 2017-09-14 | 2024-11-19 | 辉瑞公司 | 具有电子读出的复用生物测定装置 |
| US10549275B2 (en) | 2017-09-14 | 2020-02-04 | Lucira Health, Inc. | Multiplexed biological assay device with electronic readout |
| US20200392562A1 (en) * | 2017-12-22 | 2020-12-17 | Thermo Fisher Scientific Baltics Uab | Polymerase chain reaction composition comprising amines |
| GB2570157A (en) * | 2018-01-15 | 2019-07-17 | Dnanudge Ltd | Molecule detection |
| JP2021521462A (ja) | 2018-03-29 | 2021-08-26 | ファウンデーション オブ イノベーティブ ニュー ダイアグノスティクス | 抗体または抗体の組み合わせおよび対象の尿試料中のマイコバクテリウム関連抗原の検出のためのその使用 |
| GB201809420D0 (en) * | 2018-06-08 | 2018-07-25 | Imperial Innovations Ltd | Devices and method for detecting an amplification event |
| US10467679B1 (en) | 2019-04-15 | 2019-11-05 | Dnanudge Limited | Product recommendation device and method |
| USD910200S1 (en) | 2018-12-21 | 2021-02-09 | Lucira Health, Inc. | Test tube |
| US10811140B2 (en) | 2019-03-19 | 2020-10-20 | Dnanudge Limited | Secure set-up of genetic related user account |
| US10699806B1 (en) | 2019-04-15 | 2020-06-30 | Dnanudge Limited | Monitoring system, wearable monitoring device and method |
| WO2021025455A1 (ko) * | 2019-08-06 | 2021-02-11 | 주식회사 지엠디바이오텍 | 일체형 분자 진단 장치 |
| AU2021249488A1 (en) * | 2020-04-03 | 2022-09-08 | Diagonal Bio Ab | A method of measuring the pH of a sample |
| EP3895798A1 (en) | 2020-04-17 | 2021-10-20 | Pall Corporation | Flow cell for use in a fluid management and/or processing system |
| USD953561S1 (en) | 2020-05-05 | 2022-05-31 | Lucira Health, Inc. | Diagnostic device with LED display |
| USD962470S1 (en) | 2020-06-03 | 2022-08-30 | Lucira Health, Inc. | Assay device with LCD display |
| US20220228205A1 (en) * | 2021-01-15 | 2022-07-21 | Purdue Research Foundation | Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets |
| US20220228226A1 (en) | 2021-01-15 | 2022-07-21 | Purdue Research Foundation | Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets |
| US12292407B2 (en) * | 2021-02-24 | 2025-05-06 | Robert Bosch Gmbh | Measuring ion strength using closed-loop electrochemical ph modulation |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003073088A2 (en) * | 2001-03-09 | 2003-09-04 | Christofer Toumazou | Apparatus and method for the detection of localised fluctuactions of ionic charge by ion sensitive field effect transistors during a chemical reaction |
| WO2005021802A2 (en) * | 2003-08-29 | 2005-03-10 | Applera Corporation | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| WO2008039730A1 (en) * | 2006-09-25 | 2008-04-03 | Advanced Technology Materials, Inc. | Compositions and methods for the removal of photoresist for a wafer rework application |
| WO2008107014A1 (en) * | 2007-03-02 | 2008-09-12 | Dna Electronics Ltd | Qpcr using solid-state ph sensing |
| WO2009071664A1 (en) * | 2007-12-07 | 2009-06-11 | Emanuela Manna | Deodorizing and sanitizing compositions |
| WO2010008480A2 (en) * | 2008-06-25 | 2010-01-21 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE374833T1 (de) | 1998-11-09 | 2007-10-15 | Eiken Chemical | Verfahren zur synthese von nukleinsäuren |
| CN1254548C (zh) * | 2000-08-23 | 2006-05-03 | 宝生物工程株式会社 | 核酸扩增方法 |
| US8114591B2 (en) | 2001-03-09 | 2012-02-14 | Dna Electronics Ltd. | Sensing apparatus and method |
| AU2005323451B2 (en) | 2004-05-21 | 2010-11-18 | Qiagen Sciences Llc | Kits and processes for removing contaminants from nucleic acids in environmental and biological samples |
| EP3796002B1 (en) * | 2006-07-14 | 2023-11-22 | The Regents of The University of California | Cancer biomarkers and methods of use thereof |
| JP4918409B2 (ja) * | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| WO2010037085A1 (en) * | 2008-09-29 | 2010-04-01 | The Board Of Trustees Of The University Of Illinois | Dna sequencing and amplification systems using nanoscale field effect sensor arrays |
| JP5572943B2 (ja) * | 2008-12-17 | 2014-08-20 | 株式会社島津製作所 | 血液検体からのｄｎａ増幅方法及びｄｎａ増幅キット |
| ES2351294B8 (es) * | 2009-07-02 | 2012-06-06 | Consejo Superior De Investigaciones Científicas (Csic) | Metodo para la replicación, amplificación, o secuenciación de un adn molde. |
| GB201112140D0 (en) | 2011-07-14 | 2011-08-31 | Dna Electronics | Nucleic acid amplification |
-
2011
- 2011-07-14 GB GBGB1112140.7A patent/GB201112140D0/en not_active Ceased
-
2012
- 2012-07-16 EP EP12737335.5A patent/EP2732050B1/en active Active
- 2012-07-16 JP JP2014519635A patent/JP6139519B2/ja active Active
- 2012-07-16 WO PCT/GB2012/051694 patent/WO2013008042A1/en not_active Ceased
- 2012-07-16 CN CN201280034733.6A patent/CN103687962B/zh active Active
- 2012-07-16 ES ES12737335.5T patent/ES2656856T3/es active Active
- 2012-07-16 GB GB1212637.1A patent/GB2494239B/en active Active
- 2012-07-16 US US14/232,817 patent/US9469870B2/en active Active
-
2016
- 2016-09-30 US US15/282,879 patent/US9840733B2/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003073088A2 (en) * | 2001-03-09 | 2003-09-04 | Christofer Toumazou | Apparatus and method for the detection of localised fluctuactions of ionic charge by ion sensitive field effect transistors during a chemical reaction |
| WO2005021802A2 (en) * | 2003-08-29 | 2005-03-10 | Applera Corporation | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| WO2008039730A1 (en) * | 2006-09-25 | 2008-04-03 | Advanced Technology Materials, Inc. | Compositions and methods for the removal of photoresist for a wafer rework application |
| WO2008107014A1 (en) * | 2007-03-02 | 2008-09-12 | Dna Electronics Ltd | Qpcr using solid-state ph sensing |
| WO2009071664A1 (en) * | 2007-12-07 | 2009-06-11 | Emanuela Manna | Deodorizing and sanitizing compositions |
| WO2010008480A2 (en) * | 2008-06-25 | 2010-01-21 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
Non-Patent Citations (3)
| Title |
|---|
| Loop-mediated isothermal amplification of DNA;Notomi, Tsugunori, et al.;《Nucleic acids research》;20001201;第28卷(第12期);e63 * |
| Protons and single nucleotide polymorphism detection: A simple use for the Ion Sensitive Field Effect Transistor;Purushothaman S et al;《Sensors and Actuators B: Chemical》;20060426;第114卷(第2期);第964-968页 * |
| Real-time monitoring of DNA polymerase reactions by a micro ISFET pH sensor;Sakurai et al;《Analytical chemistry 》;19920101;第64卷(第17期);第1996-1997页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2494239B (en) | 2014-05-21 |
| US20170016058A1 (en) | 2017-01-19 |
| GB2494239A (en) | 2013-03-06 |
| CN103687962A (zh) | 2014-03-26 |
| GB201212637D0 (en) | 2012-08-29 |
| ES2656856T3 (es) | 2018-02-28 |
| US20140170661A1 (en) | 2014-06-19 |
| JP2014520544A (ja) | 2014-08-25 |
| EP2732050B1 (en) | 2017-11-15 |
| WO2013008042A1 (en) | 2013-01-17 |
| US9840733B2 (en) | 2017-12-12 |
| GB201112140D0 (en) | 2011-08-31 |
| JP6139519B2 (ja) | 2017-05-31 |
| US9469870B2 (en) | 2016-10-18 |
| EP2732050A1 (en) | 2014-05-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: London, England Patentee after: DNAe Group Holdings Limited Address before: London, England Patentee before: DNA Electronics L. |
|
| CP01 | Change in the name or title of a patent holder |