ES2656098T3 - Método para el aislamiento de células - Google Patents
Método para el aislamiento de células Download PDFInfo
- Publication number
- ES2656098T3 ES2656098T3 ES14003562.7T ES14003562T ES2656098T3 ES 2656098 T3 ES2656098 T3 ES 2656098T3 ES 14003562 T ES14003562 T ES 14003562T ES 2656098 T3 ES2656098 T3 ES 2656098T3
- Authority
- ES
- Spain
- Prior art keywords
- lysis
- samples
- sample
- pbs
- pellet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title abstract description 4
- 238000002955 isolation Methods 0.000 title abstract description 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 abstract description 6
- 230000001580 bacterial effect Effects 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 4
- 238000005119 centrifugation Methods 0.000 abstract description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 description 8
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 8
- 239000002953 phosphate buffered saline Substances 0.000 description 8
- 239000008188 pellet Substances 0.000 description 7
- 239000011159 matrix material Substances 0.000 description 6
- 239000012139 lysis buffer Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 241000186779 Listeria monocytogenes Species 0.000 description 3
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- NJMWOUFKYKNWDW-UHFFFAOYSA-N 1-ethyl-3-methylimidazolium Chemical compound CCN1C=C[N+](C)=C1 NJMWOUFKYKNWDW-UHFFFAOYSA-N 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- VASPYXGQVWPGAB-UHFFFAOYSA-M 1-ethyl-3-methylimidazol-3-ium;thiocyanate Chemical compound [S-]C#N.CCN1C=C[N+](C)=C1 VASPYXGQVWPGAB-UHFFFAOYSA-M 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 239000002608 ionic liquid Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000021056 liquid food Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000021055 solid food Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09007959 | 2009-06-18 | ||
| EP09007959 | 2009-06-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2656098T3 true ES2656098T3 (es) | 2018-02-23 |
Family
ID=42313799
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES10723931.1T Active ES2529215T3 (es) | 2009-06-18 | 2010-05-31 | Método para el aislamiento de células |
| ES14003562.7T Active ES2656098T3 (es) | 2009-06-18 | 2010-05-31 | Método para el aislamiento de células |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES10723931.1T Active ES2529215T3 (es) | 2009-06-18 | 2010-05-31 | Método para el aislamiento de células |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20120122110A1 (enExample) |
| EP (2) | EP2853587B1 (enExample) |
| JP (1) | JP2012529894A (enExample) |
| CN (1) | CN102459567B (enExample) |
| ES (2) | ES2529215T3 (enExample) |
| PL (1) | PL2443226T3 (enExample) |
| WO (1) | WO2010145754A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2656947T3 (es) | 2011-04-27 | 2018-03-01 | Merck Patent Gmbh | Método para lisar células |
| GB201617713D0 (en) | 2016-10-19 | 2016-11-30 | Q-Linea Ab | Method for recovering microbial cells |
| CN108949631A (zh) * | 2018-07-30 | 2018-12-07 | 骆奇 | 一种核孔膜在肠道内容物分离中的应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04228097A (ja) * | 1990-07-02 | 1992-08-18 | Toyo Ink Mfg Co Ltd | ミルク試料からの細胞分離および濃縮方法とそのキット |
| US5346994A (en) * | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| CN1226421C (zh) * | 2003-10-21 | 2005-11-09 | 南京农业大学 | 松材线虫检测试剂盒及其检测方法 |
| AT501194A1 (de) * | 2004-12-30 | 2006-07-15 | Thomas Dr Schlederer | Verfahren zur isolierung von zellen und viren |
| EP1882738A4 (en) * | 2005-05-20 | 2009-02-25 | Arkray Inc | METHOD FOR OBTAINING MICROORGANISMS AND NUCLEIC ACIDS USING FINE PARTICLES AND FOR THE METHOD OF USING THE KIT |
| ATE517192T1 (de) | 2006-08-10 | 2011-08-15 | Merck Patent Gmbh | Verfahren zur isolierung von zellen |
-
2010
- 2010-05-31 CN CN201080027681.0A patent/CN102459567B/zh active Active
- 2010-05-31 PL PL10723931T patent/PL2443226T3/pl unknown
- 2010-05-31 ES ES10723931.1T patent/ES2529215T3/es active Active
- 2010-05-31 EP EP14003562.7A patent/EP2853587B1/en active Active
- 2010-05-31 JP JP2012515373A patent/JP2012529894A/ja active Pending
- 2010-05-31 EP EP10723931.1A patent/EP2443226B1/en active Active
- 2010-05-31 WO PCT/EP2010/003295 patent/WO2010145754A1/en not_active Ceased
- 2010-05-31 US US13/378,690 patent/US20120122110A1/en not_active Abandoned
- 2010-05-31 ES ES14003562.7T patent/ES2656098T3/es active Active
-
2013
- 2013-10-21 US US14/058,772 patent/US20140045189A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP2853587B1 (en) | 2017-10-11 |
| US20140045189A1 (en) | 2014-02-13 |
| EP2443226B1 (en) | 2014-11-19 |
| ES2529215T3 (es) | 2015-02-18 |
| JP2012529894A (ja) | 2012-11-29 |
| EP2853587A1 (en) | 2015-04-01 |
| US20120122110A1 (en) | 2012-05-17 |
| CN102459567A (zh) | 2012-05-16 |
| WO2010145754A1 (en) | 2010-12-23 |
| CN102459567B (zh) | 2016-03-09 |
| PL2443226T3 (pl) | 2015-04-30 |
| EP2443226A1 (en) | 2012-04-25 |
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