CN102459567B - 分离细胞的方法 - Google Patents

分离细胞的方法 Download PDF

Info

Publication number
CN102459567B
CN102459567B CN201080027681.0A CN201080027681A CN102459567B CN 102459567 B CN102459567 B CN 102459567B CN 201080027681 A CN201080027681 A CN 201080027681A CN 102459567 B CN102459567 B CN 102459567B
Authority
CN
China
Prior art keywords
cells
methylimidazole
sample
butyl
ethyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201080027681.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN102459567A (zh
Inventor
P·罗斯马尼特
P·J·梅斯特
M·瓦格纳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of CN102459567A publication Critical patent/CN102459567A/zh
Application granted granted Critical
Publication of CN102459567B publication Critical patent/CN102459567B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN201080027681.0A 2009-06-18 2010-05-31 分离细胞的方法 Active CN102459567B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09007959 2009-06-18
EP09007959.1 2009-06-18
PCT/EP2010/003295 WO2010145754A1 (en) 2009-06-18 2010-05-31 Method for isolating cells

Publications (2)

Publication Number Publication Date
CN102459567A CN102459567A (zh) 2012-05-16
CN102459567B true CN102459567B (zh) 2016-03-09

Family

ID=42313799

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080027681.0A Active CN102459567B (zh) 2009-06-18 2010-05-31 分离细胞的方法

Country Status (7)

Country Link
US (2) US20120122110A1 (enExample)
EP (2) EP2443226B1 (enExample)
JP (1) JP2012529894A (enExample)
CN (1) CN102459567B (enExample)
ES (2) ES2529215T3 (enExample)
PL (1) PL2443226T3 (enExample)
WO (1) WO2010145754A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6147730B2 (ja) 2011-04-27 2017-06-14 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ 細胞を溶解する方法
GB201617713D0 (en) 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
CN108949631A (zh) * 2018-07-30 2018-12-07 骆奇 一种核孔膜在肠道内容物分离中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006069413A3 (de) * 2004-12-30 2006-08-24 Thomas Schlederer Verfahren zur isolierung von zellen und viren
WO2008017097A1 (en) * 2006-08-10 2008-02-14 Merck Patent Gmbh Method for isolating cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04228097A (ja) * 1990-07-02 1992-08-18 Toyo Ink Mfg Co Ltd ミルク試料からの細胞分離および濃縮方法とそのキット
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
CN1226421C (zh) * 2003-10-21 2005-11-09 南京农业大学 松材线虫检测试剂盒及其检测方法
US20090311770A1 (en) * 2005-05-20 2009-12-17 Arkray, Inc. Method of collecting microorganisms using fine particles, method of collecting nucleic acids using fine particles, and kits for use in the these methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006069413A3 (de) * 2004-12-30 2006-08-24 Thomas Schlederer Verfahren zur isolierung von zellen und viren
WO2008017097A1 (en) * 2006-08-10 2008-02-14 Merck Patent Gmbh Method for isolating cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Isolation and Identification of Pathogenic Yersinia enterocolitica from Meat and Poultry Products.;Johnson Jennifer L.;《USDA/FSIS Microbiological Laboratory Guidebook》;19980101;9-6、9-26 *

Also Published As

Publication number Publication date
JP2012529894A (ja) 2012-11-29
EP2443226B1 (en) 2014-11-19
US20140045189A1 (en) 2014-02-13
CN102459567A (zh) 2012-05-16
ES2529215T3 (es) 2015-02-18
ES2656098T3 (es) 2018-02-23
WO2010145754A1 (en) 2010-12-23
EP2853587B1 (en) 2017-10-11
EP2443226A1 (en) 2012-04-25
US20120122110A1 (en) 2012-05-17
EP2853587A1 (en) 2015-04-01
PL2443226T3 (pl) 2015-04-30

Similar Documents

Publication Publication Date Title
US9328326B2 (en) Method for isolating cells
Hazeleger et al. Temperature-dependent membrane fatty acid and cell physiology changes in coccoid forms of Campylobacter jejuni
EP2304028B1 (en) Methods and kits for extraction of dna
Rao et al. Induction, resuscitation and quantitative real-time polymerase chain reaction analyses of viable but nonculturable Vibrio vulnificus in artificial sea water
DK2732024T3 (en) CULTURE MEDIUM, METHOD FOR CULTIVATING LISTERIA, AND METHOD FOR DETERMINING LISTERIA
US20130337462A1 (en) Extraction of nucleic acids
Raissy et al. Occurrence of Vibrio spp., Aeromonas hydrophila, Escherichia coli and Campylobacter spp. in crayfish (Astacus leptodactylus) from Iran
US9493736B2 (en) Method for lysing cells
CN102459567B (zh) 分离细胞的方法
Boyacı et al. Expanding horizons of active packaging: Design of consumer-controlled release systems helps risk management of susceptible individuals
Yehia et al. Prevalence of Listeria species in some foods and their rapid identification
Basra et al. Isolation and characterization of a novel bacteriophage against Mycobacterium avium subspecies paratuberculosis
CA2420354C (en) Method to selectively inhibit bacterial growth using an aminoalkyl phosphonic selective agent
JP5827948B2 (ja) ウイルスを単離する方法
Opet et al. Efficacy of coating activated carbon with milk proteins to prevent binding of bacterial cells from foods for PCR detection
Sosa et al. Utilization of agro-industrial byproducts for bacteriocin production using Enterococcus spp. strains isolated from patagonian marine invertebrates
Mehmeti et al. Comparison of three methods for determination of protein concentration in lactic acid bacteria for proteomics studies
Liu Molecular food microbiology
CN102061303A (zh) 两种海洋动物抗菌肽基因的融合表达产物及其制备方法
JPH10500573A (ja) 複合マトリックスからの汚染微生物の迅速な分離および同定方法
Tyagi et al. Isolation of Bacterial RNA from Foods Inoculated with Pathogens
Rossmanith et al. Sample preparation for the detection of foodborne pathogens by molecular biological methods
Dev et al. EFFECT OF CYSTEINE AND DIFFERENT BUFFER MEDIA ON MEMBRANE PROTEIN EXTRACTION FROM LACTIC ACID BACTERIA.
FR2921669A1 (fr) Milieu de culture de microorganismes comprenant un inhibiteur de bacteriocines.
Kase et al. Nucleic acid sample preparation from dairy products and milk

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant