ES2621663T3 - Método de conservación de muestras y sustrato de conservación de muestras - Google Patents
Método de conservación de muestras y sustrato de conservación de muestras Download PDFInfo
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- ES2621663T3 ES2621663T3 ES12846138.1T ES12846138T ES2621663T3 ES 2621663 T3 ES2621663 T3 ES 2621663T3 ES 12846138 T ES12846138 T ES 12846138T ES 2621663 T3 ES2621663 T3 ES 2621663T3
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H13/00—Pulp or paper, comprising synthetic cellulose or non-cellulose fibres or web-forming material
- D21H13/36—Inorganic fibres or flakes
- D21H13/38—Inorganic fibres or flakes siliceous
- D21H13/40—Inorganic fibres or flakes siliceous vitreous, e.g. mineral wool, glass fibres
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/20—Macromolecular organic compounds
- D21H17/21—Macromolecular organic compounds of natural origin; Derivatives thereof
- D21H17/22—Proteins
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/20—Macromolecular organic compounds
- D21H17/21—Macromolecular organic compounds of natural origin; Derivatives thereof
- D21H17/24—Polysaccharides
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/20—Macromolecular organic compounds
- D21H17/33—Synthetic macromolecular compounds
- D21H17/46—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D21H17/52—Epoxy resins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/126—Paper
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Inorganic Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Paper (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB201118731 | 2011-10-31 | ||
| GB1118731.7A GB2496122A (en) | 2011-10-31 | 2011-10-31 | Biological sample preservation on paper |
| PCT/SE2012/051168 WO2013066249A1 (en) | 2011-10-31 | 2012-10-29 | Sample preservation method and sample preservation substrate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2621663T3 true ES2621663T3 (es) | 2017-07-04 |
Family
ID=45375549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12846138.1T Active ES2621663T3 (es) | 2011-10-31 | 2012-10-29 | Método de conservación de muestras y sustrato de conservación de muestras |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10371608B2 (enExample) |
| EP (1) | EP2773956B1 (enExample) |
| JP (1) | JP6143765B2 (enExample) |
| ES (1) | ES2621663T3 (enExample) |
| GB (1) | GB2496122A (enExample) |
| WO (1) | WO2013066249A1 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9044738B2 (en) | 2012-04-30 | 2015-06-02 | General Electric Company | Methods and compositions for extraction and storage of nucleic acids |
| US9480966B2 (en) | 2012-04-30 | 2016-11-01 | General Electric Company | Substrates and methods for collection, stabilization and elution of biomolecules |
| US9040679B2 (en) | 2012-04-30 | 2015-05-26 | General Electric Company | Methods and compositions for extraction and storage of nucleic acids |
| US9040675B2 (en) | 2012-04-30 | 2015-05-26 | General Electric Company | Formulations for nucleic acid stabilization on solid substrates |
| WO2013179141A1 (en) * | 2012-05-31 | 2013-12-05 | University Of Oslo | Sampling medium |
| GB2538955A (en) * | 2015-05-29 | 2016-12-07 | Ge Healthcare Uk Ltd | Improvements in and relating to extracting biological analytes |
| ES2912965T3 (es) | 2015-09-09 | 2022-05-30 | Drawbridge Health Inc | Dispositivos para la recopilación, estabilización y conservación de muestras |
| CN210383905U (zh) | 2017-01-10 | 2020-04-24 | 集联健康有限公司 | 一种用于从受试者收集流体样品的装置以及运输套筒 |
| WO2018155410A1 (ja) * | 2017-02-22 | 2018-08-30 | 公益財団法人ヒューマンサイエンス振興財団 | 乾燥血液試料保存基材 |
| JP6904561B2 (ja) * | 2017-02-22 | 2021-07-21 | 独立行政法人国立病院機構 | 乾燥血液試料保存基材 |
| JP7270264B2 (ja) * | 2018-08-09 | 2023-05-10 | 独立行政法人国立病院機構 | 乾燥血液試料保存基材 |
| AU2021364335A1 (en) * | 2020-10-20 | 2023-06-22 | Definitek, Inc. | Quantification of previously undetectable quantities |
| AU2024232333A1 (en) * | 2023-03-08 | 2025-09-25 | Imyoo Inc. | Compositions and methods for whole blood preservation |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3070486A (en) | 1958-12-15 | 1962-12-25 | Ohio Commw Eng Co | Cigarette paper products comprising water insoluble dextran |
| US3300474A (en) | 1964-02-12 | 1967-01-24 | Pharmacia Ab | Sucrose ether copolymerizates |
| CA928198A (en) | 1968-07-15 | 1973-06-12 | Boehringer Mannheim G.M.B.H. | Storable blood and serum samples and a process for their preparation |
| AU2919171A (en) * | 1970-05-27 | 1972-11-30 | Pharmacia Ab | A method for carrying out tests, a reagent andan additive for carrying out the method |
| EP0325413A3 (en) * | 1988-01-19 | 1990-10-24 | Syntex (U.S.A.) Inc. | Method and device for separating plasma from red cells |
| JP2728757B2 (ja) | 1988-02-11 | 1998-03-18 | キューノ，インコーポレーテッド | 変性多糖支持体の親和性マトリックス |
| JPH01291164A (ja) * | 1988-05-19 | 1989-11-22 | Fujirebio Inc | 標準瀘紙用組成物 |
| US5252489A (en) * | 1989-01-17 | 1993-10-12 | Macri James N | Down syndrome screening method utilizing dried blood samples |
| CA2127605A1 (en) * | 1993-12-23 | 1995-06-24 | Peter J. Degen | Affinity separation method |
| US5725774A (en) * | 1995-04-07 | 1998-03-10 | Lxn Corp. | Whole blood separation method and devices using the same |
| US6309887B1 (en) * | 1998-01-27 | 2001-10-30 | Flexsite Diagnostics, Inc. | Filter paper treatment for improved diagnostic assays |
| DE10036907B4 (de) * | 2000-07-28 | 2012-03-22 | Xantec Bioanalytics Gmbh | Verfahren zur Herstellung einer Beschichtung auf einem mit Gold bedampften Glassubstrat, Beschichtung hergestellt nach diesem Verfahren und deren Verwendung |
| EP1423514A2 (en) * | 2001-09-05 | 2004-06-02 | WHATMAN plc | Stable storage of proteins |
| JP2009092651A (ja) * | 2007-09-20 | 2009-04-30 | Fujifilm Corp | 担体およびその製造方法 |
| WO2009090174A1 (en) * | 2008-01-17 | 2009-07-23 | Whatman Inc | Composite membrane blends comprising ionic branch polymers and methods of use |
| US20100209957A1 (en) * | 2008-06-20 | 2010-08-19 | Genvault Corporation | Biosample storage devices and methods of use thereof |
| BRPI0923565A2 (pt) * | 2008-12-23 | 2016-01-26 | 3M Innovative Properties Co | artigo não tecido funcionalizado |
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| EP2773956A4 (en) | 2015-06-17 |
| JP2014535011A (ja) | 2014-12-25 |
| US20140302521A1 (en) | 2014-10-09 |
| JP6143765B2 (ja) | 2017-06-07 |
| EP2773956B1 (en) | 2017-03-15 |
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