ES2562789T3 - Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3' - Google Patents

Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3' Download PDF

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Publication number
ES2562789T3
ES2562789T3 ES08785392.5T ES08785392T ES2562789T3 ES 2562789 T3 ES2562789 T3 ES 2562789T3 ES 08785392 T ES08785392 T ES 08785392T ES 2562789 T3 ES2562789 T3 ES 2562789T3
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Prior art keywords
sequence
blocking oligonucleotide
oligonucleotide
target
target sequence
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ES08785392.5T
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English (en)
Spanish (es)
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Nicolas Newton
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
ES08785392.5T 2007-08-08 2008-08-07 Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3' Active ES2562789T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96408907P 2007-08-08 2007-08-08
US964089P 2007-08-08
PCT/EP2008/006476 WO2009019008A1 (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Publications (1)

Publication Number Publication Date
ES2562789T3 true ES2562789T3 (es) 2016-03-08

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ID=39942715

Family Applications (1)

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ES08785392.5T Active ES2562789T3 (es) 2007-08-08 2008-08-07 Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3'

Country Status (7)

Country Link
US (1) US8071338B2 (show.php)
EP (1) EP2173910B1 (show.php)
JP (1) JP5524838B2 (show.php)
CN (1) CN101778952B (show.php)
CA (1) CA2693384C (show.php)
ES (1) ES2562789T3 (show.php)
WO (1) WO2009019008A1 (show.php)

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US8455190B2 (en) 2007-08-01 2013-06-04 Dana-Farber Cancer Institute, Inc. Enrichment of a target sequence
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
WO2011056687A2 (en) 2009-10-27 2011-05-12 Swift Biosciences, Inc. Polynucleotide primers and probes
ES2665500T3 (es) * 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia
KR101851382B1 (ko) * 2010-09-10 2018-04-23 가부시키가이샤 엘에스아이 메디엔스 광을 이용한 핵산의 증폭 억제 방법 및 고감도의 선택적 핵산 증폭 방법
US9284602B2 (en) 2010-10-27 2016-03-15 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
CA2828535A1 (en) * 2011-02-28 2012-09-07 Transgenomic, Inc. Kit and method for sequencing a target dna in a mixed population
WO2012135664A2 (en) * 2011-03-31 2012-10-04 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex cold-pcr
US9796748B2 (en) 2011-05-02 2017-10-24 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
EP3382039B1 (en) * 2011-05-04 2023-09-13 Biocept, Inc. Methods for detecting nucleic acid sequence variants
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
EP2758458A4 (en) 2011-07-10 2015-10-21 Harvard College COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS
EP3263718A1 (en) 2011-09-16 2018-01-03 Lexogen GmbH Nucleic acid transcription method
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
US9260714B2 (en) 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
WO2014177540A1 (en) * 2013-04-29 2014-11-06 Qiagen Marseille A method for dna amplification with a blocking oligonucleotide
AU2014336987A1 (en) * 2013-10-19 2016-04-14 Trovagene, Inc. Detecting mutations in disease over time
JP2017515484A (ja) * 2014-05-19 2017-06-15 ウィリアム マーシュ ライス ユニバーシティＷｉｌｌｉａｍ Ｍａｒｓｈ Ｒｉｃｅ Ｕｎｉｖｅｒｓｉｔｙ 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
JP6770957B2 (ja) 2014-08-07 2020-10-21 ファーマシスト リミテッドＰｈａｒｍａｓｓｉｓｔ Ｌｔｄ 試料中のｐｉｋ３ｃａ突然変異状態を決定する方法
EA037920B1 (ru) * 2014-09-26 2021-06-07 Янссен Фармацевтика Нв Использование панелей мутантных генов fgfr для выявления онкологических пациентов, которые будут поддаваться лечению ингибитором fgfr
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
CN109477111B (zh) * 2016-06-07 2023-12-15 塞弗德公司 热稳定聚合酶抑制剂组合物和方法
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
CN111683958A (zh) 2018-02-20 2020-09-18 威廉马歇莱思大学 使用多重抑制探针置换扩增进行等位基因富集的系统和方法
EP3682030B1 (en) 2018-07-19 2021-05-19 Biofidelity Ltd Improved polynucleotide sequence detection method
SG11202012758WA (en) * 2018-12-17 2021-01-28 Illumina Cambridge Ltd Primer oligonucleotide for sequencing
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
US20240401122A1 (en) * 2021-09-13 2024-12-05 Nexgen Cancer Detection Llc Methods for detecting a target polynucleotide in a sample

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CA2163393C (en) 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US5849497A (en) 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
FR2779154B1 (fr) 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
EP1141384A2 (en) * 1999-01-06 2001-10-10 Cornell Research Foundation, Inc. Method for accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
ATE476439T1 (de) 2004-09-10 2010-08-15 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)

Also Published As

Publication number Publication date
CA2693384A1 (en) 2009-02-12
JP5524838B2 (ja) 2014-06-18
US20090053720A1 (en) 2009-02-26
EP2173910B1 (en) 2015-12-09
CA2693384C (en) 2015-04-14
US8071338B2 (en) 2011-12-06
JP2010535480A (ja) 2010-11-25
EP2173910A1 (en) 2010-04-14
CN101778952B (zh) 2016-05-25
HK1146094A1 (zh) 2011-05-13
CN101778952A (zh) 2010-07-14
WO2009019008A1 (en) 2009-02-12

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