ES2530752T3 - Procedimientos y composiciones para la biosíntesis recombinante de n-alcanos - Google Patents
Procedimientos y composiciones para la biosíntesis recombinante de n-alcanos Download PDFInfo
- Publication number
- ES2530752T3 ES2530752T3 ES13152069T ES13152069T ES2530752T3 ES 2530752 T3 ES2530752 T3 ES 2530752T3 ES 13152069 T ES13152069 T ES 13152069T ES 13152069 T ES13152069 T ES 13152069T ES 2530752 T3 ES2530752 T3 ES 2530752T3
- Authority
- ES
- Spain
- Prior art keywords
- genomanipulated
- recombinant
- photosynthetic microbe
- alkanes
- carbon dioxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C11/00—Aliphatic unsaturated hydrocarbons
- C07C11/02—Alkenes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C47/00—Compounds having —CHO groups
- C07C47/02—Saturated compounds having —CHO groups bound to acyclic carbon atoms or to hydrogen
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C9/00—Aliphatic saturated hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10G—CRACKING HYDROCARBON OILS; PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVE HYDROGENATION, OLIGOMERISATION, POLYMERISATION; RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, OR GASES; REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS; REFORMING OF NAPHTHA; MINERAL WAXES
- C10G2300/00—Aspects relating to hydrocarbon processing covered by groups C10G1/00 - C10G99/00
- C10G2300/40—Characteristics of the process deviating from typical ways of processing
- C10G2300/4043—Limiting CO2 emissions
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P30/00—Technologies relating to oil refining and petrochemical industry
- Y02P30/40—Ethylene production
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Cultivation Of Plants (AREA)
- Cosmetics (AREA)
Abstract
Un procedimiento para producir hidrocarburos, que comprende: (i) cultivar un microbio fotosintético genomanipulado en un medio de cultivo, donde dicho microbio fotosintético genomanipulado comprende una enzima acil-ACP reductasa recombinante y una enzima alcanal monooxigenasa decarboxilativa recombinante; y (ii) exponer dicho microbio fotosintético genomanipulado a la luz y al dióxido de carbono, donde dicha exposición da como resultado la conversión de dicho dióxido de carbono mediante dicho microbio fotosintético genomanipulado en n-alcanos, y donde la cantidad de dichos n-alcanos producida es al menos de 0,1% del peso de células secas y al menos dos veces la cantidad producida por un microbio fotosintético idéntico en todo lo demás, cultivada en condiciones idénticas, pero que carece de dichas enzimas acil-ACP reductasa recombinante y alcanal monooxigenasa decarboxilativa.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22446309P | 2009-07-09 | 2009-07-09 | |
US22893709P | 2009-07-27 | 2009-07-27 | |
US12/759,657 US7794969B1 (en) | 2009-07-09 | 2010-04-13 | Methods and compositions for the recombinant biosynthesis of n-alkanes |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2530752T3 true ES2530752T3 (es) | 2015-03-05 |
Family
ID=42710928
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES13152069T Active ES2530752T3 (es) | 2009-07-09 | 2010-07-09 | Procedimientos y composiciones para la biosíntesis recombinante de n-alcanos |
ES10776070T Active ES2413484T3 (es) | 2009-07-09 | 2010-07-09 | Procedimientos y composiciones para la biosíntesis recombinante de n-alcanos |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES10776070T Active ES2413484T3 (es) | 2009-07-09 | 2010-07-09 | Procedimientos y composiciones para la biosíntesis recombinante de n-alcanos |
Country Status (12)
Country | Link |
---|---|
US (6) | US7794969B1 (es) |
EP (3) | EP2584032B1 (es) |
CN (2) | CN102597248B (es) |
AU (1) | AU2010246473B2 (es) |
BR (1) | BR112012000355A2 (es) |
CA (1) | CA2766204A1 (es) |
EA (1) | EA201270151A1 (es) |
ES (2) | ES2530752T3 (es) |
IL (1) | IL217389A0 (es) |
MX (1) | MX2012000448A (es) |
MY (1) | MY156452A (es) |
WO (1) | WO2011006137A2 (es) |
Families Citing this family (41)
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US8354514B2 (en) * | 2007-01-12 | 2013-01-15 | Lawrence Livermore National Security, Llc | Multiplex detection of agricultural pathogens |
MX343063B (es) * | 2008-05-16 | 2016-10-21 | Reg Life Sciences Llc | Metodos y composiciones para producir hidrocarburos. |
US8394621B2 (en) | 2008-10-23 | 2013-03-12 | Matrix Genetrics, LLC | Modified photosynthetic microorganisms for producing triglycerides |
US8809037B2 (en) | 2008-10-24 | 2014-08-19 | Bioprocessh20 Llc | Systems, apparatuses and methods for treating wastewater |
CN102264910B (zh) | 2008-10-28 | 2015-08-26 | Reg生命科学有限责任公司 | 用于产生脂肪醇的方法和组合物 |
WO2013148348A1 (en) * | 2012-03-28 | 2013-10-03 | Kiverdi, Inc. | Engineered co2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same |
US9957534B2 (en) | 2008-11-06 | 2018-05-01 | Kiverdi, Inc. | Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same |
US8445248B2 (en) * | 2008-11-17 | 2013-05-21 | Board Of Regents Of The Nevada System Of Higher Education On Behalf Of The University Of Nevada, Reno | Hydrocarbon-forming oxidative decarbonylase enzyme, hydrocarbons produced thereby, and method of use |
US8835137B2 (en) * | 2008-12-23 | 2014-09-16 | Matrix Genetics, Llc | Modified photosynthetic microorganisms with reduced glycogen and their use in producing carbon-based products |
US7955820B1 (en) * | 2009-07-09 | 2011-06-07 | Joule Unlimited, Inc. | Methods and compositions for the recombinant biosynthesis of n-alkanes |
US7794969B1 (en) * | 2009-07-09 | 2010-09-14 | Joule Unlimited, Inc. | Methods and compositions for the recombinant biosynthesis of n-alkanes |
US20120077251A1 (en) | 2010-09-14 | 2012-03-29 | C/O Joule Unlimited Technologies, Inc. | Methods and Compositions for Targeting Heterologous Integral Membrane Proteins to the Cyanobacterial Plasma Membrane |
US20110124071A1 (en) * | 2009-11-17 | 2011-05-26 | LS9, Inc | Methods and compositions for producing hydrocarbons |
US8980613B2 (en) | 2010-04-06 | 2015-03-17 | Matrix Genetics, Llc | Modified photosynthetic microorganisms for producing lipids |
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US20140004580A1 (en) * | 2010-12-20 | 2014-01-02 | Matrix Genetics, Llc | Modified photosynthetic microorganisms for producing lipids |
US9523096B2 (en) | 2010-12-20 | 2016-12-20 | Matrix Genetics, Llc | Modified photosynthetic microorganisms for producing lipids |
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CN103215315B (zh) * | 2013-04-15 | 2015-05-20 | 上海工业生物技术研发中心 | 利用蓝藻生产异戊二烯 |
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CN105462905A (zh) * | 2014-09-02 | 2016-04-06 | 中国科学院过程工程研究所 | 一种微生物基因改造方法、用于生产高级脂肪醇、高级脂肪烃的基因工程菌及其应用 |
WO2016040649A1 (en) * | 2014-09-12 | 2016-03-17 | Coffa Gianguido | Genetically modified microbes for the biological conversion of carbonaceous materials to alkanes |
MX2017008289A (es) | 2014-12-23 | 2017-10-02 | Algenol Biotech LLC | Metodos para aumentar la estabilidad de la produccion de compuestos en celulas huesped microbianas. |
US10138489B2 (en) | 2016-10-20 | 2018-11-27 | Algenol Biotech LLC | Cyanobacterial strains capable of utilizing phosphite |
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US7794969B1 (en) * | 2009-07-09 | 2010-09-14 | Joule Unlimited, Inc. | Methods and compositions for the recombinant biosynthesis of n-alkanes |
US7955820B1 (en) * | 2009-07-09 | 2011-06-07 | Joule Unlimited, Inc. | Methods and compositions for the recombinant biosynthesis of n-alkanes |
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HU231091B1 (hu) * | 2009-09-30 | 2020-07-28 | Mol Magyar Olaj- És Gázipari Nyilvánosan Működő Részvénytársaság | Belső égésű motoroknál használható hajtóanyagok és hajtóanyag-adalékok, valamint eljárás ezek előállítására |
-
2010
- 2010-04-13 US US12/759,657 patent/US7794969B1/en not_active Expired - Fee Related
- 2010-07-09 MX MX2012000448A patent/MX2012000448A/es active IP Right Grant
- 2010-07-09 ES ES13152069T patent/ES2530752T3/es active Active
- 2010-07-09 EA EA201270151A patent/EA201270151A1/ru unknown
- 2010-07-09 WO PCT/US2010/041619 patent/WO2011006137A2/en active Application Filing
- 2010-07-09 EP EP13152069.4A patent/EP2584032B1/en not_active Not-in-force
- 2010-07-09 BR BR112012000355A patent/BR112012000355A2/pt not_active IP Right Cessation
- 2010-07-09 CN CN201080031073.7A patent/CN102597248B/zh not_active Expired - Fee Related
- 2010-07-09 EP EP10776070A patent/EP2307553B1/en not_active Not-in-force
- 2010-07-09 CA CA2766204A patent/CA2766204A1/en not_active Abandoned
- 2010-07-09 MY MYPI2012000092A patent/MY156452A/en unknown
- 2010-07-09 EP EP20130197830 patent/EP2787061A1/en not_active Withdrawn
- 2010-07-09 ES ES10776070T patent/ES2413484T3/es active Active
- 2010-07-09 CN CN201410718292.2A patent/CN104630279A/zh active Pending
- 2010-07-09 AU AU2010246473A patent/AU2010246473B2/en not_active Ceased
- 2010-09-10 US US12/879,862 patent/US7919303B2/en not_active Expired - Fee Related
-
2011
- 2011-03-18 US US13/051,807 patent/US8101397B2/en not_active Expired - Fee Related
- 2011-12-22 US US13/335,886 patent/US8481285B2/en not_active Expired - Fee Related
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2012
- 2012-01-05 IL IL217389A patent/IL217389A0/en unknown
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2013
- 2013-06-10 US US13/913,795 patent/US9458069B2/en not_active Expired - Fee Related
-
2016
- 2016-09-01 US US15/254,957 patent/US20170051315A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
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US8101397B2 (en) | 2012-01-24 |
IL217389A0 (en) | 2012-02-29 |
AU2010246473B2 (en) | 2011-04-28 |
US7794969B1 (en) | 2010-09-14 |
EP2584032A3 (en) | 2013-08-21 |
EA201270151A1 (ru) | 2012-06-29 |
US9458069B2 (en) | 2016-10-04 |
EP2584032A2 (en) | 2013-04-24 |
CN102597248A (zh) | 2012-07-18 |
US20110172467A1 (en) | 2011-07-14 |
US20140005439A1 (en) | 2014-01-02 |
MY156452A (en) | 2016-02-26 |
CN104630279A (zh) | 2015-05-20 |
WO2011006137A2 (en) | 2011-01-13 |
EP2787061A1 (en) | 2014-10-08 |
US20110009674A1 (en) | 2011-01-13 |
EP2307553B1 (en) | 2013-03-13 |
MX2012000448A (es) | 2012-10-05 |
EP2307553A4 (en) | 2011-09-28 |
AU2010246473A1 (en) | 2011-01-27 |
US20170051315A1 (en) | 2017-02-23 |
EP2584032B1 (en) | 2014-11-19 |
US20120095266A1 (en) | 2012-04-19 |
WO2011006137A3 (en) | 2011-03-03 |
US7919303B2 (en) | 2011-04-05 |
US8481285B2 (en) | 2013-07-09 |
BR112012000355A2 (pt) | 2015-10-13 |
CN102597248B (zh) | 2014-12-31 |
EP2307553A2 (en) | 2011-04-13 |
CA2766204A1 (en) | 2011-01-13 |
ES2413484T3 (es) | 2013-07-16 |
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