EP2584032A3 - Methods and compositions for the recombinant biosynthesis of n-alkanes - Google Patents

Methods and compositions for the recombinant biosynthesis of n-alkanes Download PDF

Info

Publication number
EP2584032A3
EP2584032A3 EP13152069.4A EP13152069A EP2584032A3 EP 2584032 A3 EP2584032 A3 EP 2584032A3 EP 13152069 A EP13152069 A EP 13152069A EP 2584032 A3 EP2584032 A3 EP 2584032A3
Authority
EP
European Patent Office
Prior art keywords
alkanes
compositions
microbes
recombinant
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP13152069.4A
Other languages
German (de)
French (fr)
Other versions
EP2584032A2 (en
EP2584032B1 (en
Inventor
Nikos Basil Reppas
Christian Perry Ridley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Joule Unlimited Technologies Inc
Original Assignee
Joule Unlimited Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joule Unlimited Technologies Inc filed Critical Joule Unlimited Technologies Inc
Priority to EP20130197830 priority Critical patent/EP2787061A1/en
Publication of EP2584032A2 publication Critical patent/EP2584032A2/en
Publication of EP2584032A3 publication Critical patent/EP2584032A3/en
Application granted granted Critical
Publication of EP2584032B1 publication Critical patent/EP2584032B1/en
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C11/00—Aliphatic unsaturated hydrocarbons
    • C07C11/02—Alkenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C47/00—Compounds having —CHO groups
    • C07C47/02—Saturated compounds having —CHO groups bound to acyclic carbon atoms or to hydrogen
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C9/00—Aliphatic saturated hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10G—CRACKING HYDROCARBON OILS; PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVE HYDROGENATION, OLIGOMERISATION, POLYMERISATION; RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, OR GASES; REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS; REFORMING OF NAPHTHA; MINERAL WAXES
    • C10G2300/00—Aspects relating to hydrocarbon processing covered by groups C10G1/00 - C10G99/00
    • C10G2300/40—Characteristics of the process deviating from typical ways of processing
    • C10G2300/4043—Limiting CO2 emissions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P30/00—Technologies relating to oil refining and petrochemical industry
    • Y02P30/40—Ethylene production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cultivation Of Plants (AREA)
  • Cosmetics (AREA)

Abstract

The present disclosure identifies methods and compositions for modifying photosynthetic microbes as hosts, such that the microbes efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules. The photosyntheric microbes are engineered to comprise a recombinant acyl-ACP reductase enzyme and a recombinant alkanal decarboxylative monooxygenase enzyme.
EP13152069.4A 2009-07-09 2010-07-09 Methods and compositions for the recombinant biosynthesis of n-alkanes Not-in-force EP2584032B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP20130197830 EP2787061A1 (en) 2009-07-09 2010-07-09 Fuel composition comprising a mixture of n-alkanes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US22446309P 2009-07-09 2009-07-09
US22893709P 2009-07-27 2009-07-27
US12/759,657 US7794969B1 (en) 2009-07-09 2010-04-13 Methods and compositions for the recombinant biosynthesis of n-alkanes
EP10776070A EP2307553B1 (en) 2009-07-09 2010-07-09 Methods and compositions for the recombinant biosynthesis of n-alkanes

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP10776070.4 Division 2010-07-09
EP10776070A Division EP2307553B1 (en) 2009-07-09 2010-07-09 Methods and compositions for the recombinant biosynthesis of n-alkanes

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP20130197830 Division EP2787061A1 (en) 2009-07-09 2010-07-09 Fuel composition comprising a mixture of n-alkanes
EP20130197830 Division-Into EP2787061A1 (en) 2009-07-09 2010-07-09 Fuel composition comprising a mixture of n-alkanes

Publications (3)

Publication Number Publication Date
EP2584032A2 EP2584032A2 (en) 2013-04-24
EP2584032A3 true EP2584032A3 (en) 2013-08-21
EP2584032B1 EP2584032B1 (en) 2014-11-19

Family

ID=42710928

Family Applications (3)

Application Number Title Priority Date Filing Date
EP13152069.4A Not-in-force EP2584032B1 (en) 2009-07-09 2010-07-09 Methods and compositions for the recombinant biosynthesis of n-alkanes
EP10776070A Not-in-force EP2307553B1 (en) 2009-07-09 2010-07-09 Methods and compositions for the recombinant biosynthesis of n-alkanes
EP20130197830 Withdrawn EP2787061A1 (en) 2009-07-09 2010-07-09 Fuel composition comprising a mixture of n-alkanes

Family Applications After (2)

Application Number Title Priority Date Filing Date
EP10776070A Not-in-force EP2307553B1 (en) 2009-07-09 2010-07-09 Methods and compositions for the recombinant biosynthesis of n-alkanes
EP20130197830 Withdrawn EP2787061A1 (en) 2009-07-09 2010-07-09 Fuel composition comprising a mixture of n-alkanes

Country Status (12)

Country Link
US (6) US7794969B1 (en)
EP (3) EP2584032B1 (en)
CN (2) CN102597248B (en)
AU (1) AU2010246473B2 (en)
BR (1) BR112012000355A2 (en)
CA (1) CA2766204A1 (en)
EA (1) EA201270151A1 (en)
ES (2) ES2530752T3 (en)
IL (1) IL217389A0 (en)
MX (1) MX2012000448A (en)
MY (1) MY156452A (en)
WO (1) WO2011006137A2 (en)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8354514B2 (en) * 2007-01-12 2013-01-15 Lawrence Livermore National Security, Llc Multiplex detection of agricultural pathogens
CA2722441C (en) * 2008-05-16 2021-01-26 Ls9, Inc. Methods and compositions for producing hydrocarbons
EP2358864B8 (en) * 2008-10-23 2017-09-13 Lumen Bioscience, Inc. Modified photosynthetic microorganisms for producing triglycerides
US8809037B2 (en) 2008-10-24 2014-08-19 Bioprocessh20 Llc Systems, apparatuses and methods for treating wastewater
KR101700296B1 (en) 2008-10-28 2017-01-26 알이지 라이프 사이언시스, 엘엘씨 Methods and compositions for producing fatty alcohols
WO2013148348A1 (en) * 2012-03-28 2013-10-03 Kiverdi, Inc. Engineered co2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same
US9957534B2 (en) 2008-11-06 2018-05-01 Kiverdi, Inc. Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same
US8445248B2 (en) * 2008-11-17 2013-05-21 Board Of Regents Of The Nevada System Of Higher Education On Behalf Of The University Of Nevada, Reno Hydrocarbon-forming oxidative decarbonylase enzyme, hydrocarbons produced thereby, and method of use
US8835137B2 (en) * 2008-12-23 2014-09-16 Matrix Genetics, Llc Modified photosynthetic microorganisms with reduced glycogen and their use in producing carbon-based products
US7955820B1 (en) * 2009-07-09 2011-06-07 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
US7794969B1 (en) * 2009-07-09 2010-09-14 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
WO2011062987A2 (en) * 2009-11-17 2011-05-26 Ls9, Inc. Methods and compositions for producing hydrocarbons
WO2011127069A1 (en) 2010-04-06 2011-10-13 Targeted Growth, Inc. Modified photosynthetic microorganisms for producing lipids
WO2012037288A2 (en) 2010-09-14 2012-03-22 Joule Unlimited Technologies, Inc. Methods and compositions for the extracellular transport of biosynthetic hydrocarbons and other molecules
US20120157717A1 (en) * 2010-09-15 2012-06-21 Ls9, Inc. Methods and compositions for producing linear alkyl benzenes
EP2655605A1 (en) * 2010-12-20 2013-10-30 Matrix Genetics, LLC Modified photosynthetic microorganisms for producing lipids
WO2012087982A2 (en) * 2010-12-20 2012-06-28 Targeted Growth, Inc. Modified photosynthetic microorganisms for producing lipids
US20120196336A1 (en) 2011-01-28 2012-08-02 Mccutchen Co. Radial counterflow reactor with applied radiant energy
US8580542B2 (en) 2011-02-10 2013-11-12 Joule Unlimited Technologies, Inc. Methods and compositions for producing alkenes of various chain length
US8993303B2 (en) 2011-02-24 2015-03-31 South Dakota State University Genetically engineered cyanobacteria
US9029124B2 (en) 2011-03-23 2015-05-12 Joule Unlimited Technologies, Inc. Photoalkanogens with increased productivity
WO2012173855A2 (en) 2011-06-10 2012-12-20 Joule Unlimited Technologies, Inc. Systems and methods for delivery of carbon dioxide, bioreactor systems, and uses thereof
US20140134672A1 (en) 2011-07-06 2014-05-15 Max B. Tuttman Bioreactors circulation apparatus, system and method
WO2013022670A1 (en) 2011-08-05 2013-02-14 Joule Unlimited Technologies, Inc. Flexible photobioreactors, systems and methods
WO2013028792A2 (en) * 2011-08-22 2013-02-28 Joule Unlimited Technologies, Inc. Improved compositions and methods for the biosynthesis of 1-alkenes in engineered microorganisms
BR112014007115A2 (en) 2011-09-27 2017-04-25 Exxonmobil Res & Eng Co acyl-acp wax ester synthases
AU2012360934A1 (en) 2011-12-30 2014-07-31 Algenol Biofuels Inc. Genetically enhanced cyanobacteria for the production of a first chemical compound harbouring Zn2+, Co2+ or Ni2+ -inducible promoters
WO2013124439A2 (en) * 2012-02-24 2013-08-29 Shell Internationale Research Maatschappij B.V. Method for preparing a hydrocarbon
WO2014039879A1 (en) 2012-09-06 2014-03-13 North Carolina State University Sequestration of carbon dioxide with hydrogen to useful products
US8846369B2 (en) 2012-12-21 2014-09-30 Algenol Biofuels Inc. Cyanobacterium sp. host cell and vector for production of chemical compounds in cyanobacterial cultures
US9157101B2 (en) 2012-12-21 2015-10-13 Algenol Biotech LLC Cyanobacterium sp. for production of compounds
MY167434A (en) * 2013-01-16 2018-08-28 Reg Life Sciences Llc Acyl-acp reductase with improved properties
US9034629B2 (en) 2013-01-25 2015-05-19 Joule Unlimited Technologies, Inc. Recombinant synthesis of medium chain-length alkanes
US20140273132A1 (en) 2013-03-15 2014-09-18 Algenol Biofuels Inc. Process for Inoculating a Bioreactor with Cyanobacteria
CN103215315B (en) * 2013-04-15 2015-05-20 上海工业生物技术研发中心 Method for producing isoprene by utilizing blue algae
WO2014198964A2 (en) * 2013-06-14 2014-12-18 Algenol Biofuels Inc. Metabolically enhanced cyanobacterial cell for the production of ethanol
CN105462905A (en) * 2014-09-02 2016-04-06 中国科学院过程工程研究所 Microorganism gene modification method, genetically engineered bacteria for producing higher aliphatic alcohol and higher aliphatic hydrocarbon and application of genetically engineered bacteria
WO2016040649A1 (en) * 2014-09-12 2016-03-17 Coffa Gianguido Genetically modified microbes for the biological conversion of carbonaceous materials to alkanes
AU2015371292A1 (en) 2014-12-23 2017-07-20 Algenol Biotech LLC Methods for increasing the stability of production of compounds in microbial host cells
US10138489B2 (en) 2016-10-20 2018-11-27 Algenol Biotech LLC Cyanobacterial strains capable of utilizing phosphite
US10537840B2 (en) 2017-07-31 2020-01-21 Vorsana Inc. Radial counterflow separation filter with focused exhaust

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008003078A2 (en) * 2006-06-29 2008-01-03 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
WO2009111513A1 (en) * 2008-03-03 2009-09-11 Joule Biotechnologies, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
WO2009140695A1 (en) * 2008-05-16 2009-11-19 Ls9, Inc. Methods and compositions for producing hydrocarbons

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014816A1 (en) 1991-02-22 1992-09-03 Calgene, Inc. Seed plant fatty acyl reductase proteins and genes
US5723747A (en) 1991-02-22 1998-03-03 Calgene, Inc. Wax esters in transformed plants
JPH07300593A (en) * 1994-03-09 1995-11-14 Idemitsu Petrochem Co Ltd Odorless liquid fuel for open flame oil cooker
US20020022239A1 (en) 1999-03-19 2002-02-21 John Clark Lagarias Phytofluors as fluorescent labels
US20040048343A1 (en) * 2002-01-19 2004-03-11 Theron Hermann Method and microorganisms for the production of 3-(2-hydroxy-3-methyl-butyrylamino)-propionic acid (hmbpa)
US7223876B2 (en) 2004-04-21 2007-05-29 Basf Aktiengesellschaft Method of separating an olefin from a gas stream
US7226857B2 (en) * 2004-07-30 2007-06-05 Micron Technology, Inc. Front-end processing of nickel plated bond pads
US7176301B2 (en) 2005-03-31 2007-02-13 Japan Tobacco Inc. Nitrate inducible promoter
EP1968994B1 (en) 2005-12-06 2013-07-03 Synthetic Genomics, Inc. Synthetic genomes
JP2009521229A (en) 2005-12-23 2009-06-04 ジェイ．クレイグ ベンター インスティチュート Installing genomes or partial genomes into cells or cell-like systems
KR20090029708A (en) 2006-05-19 2009-03-23 엘에스9, 인코포레이티드 Production of fatty acids and derivatives thereof
CN101617039A (en) * 2006-10-20 2009-12-30 代表亚利桑那大学的亚利桑那校董会 Modified cyanobacteria
US7618799B2 (en) 2007-01-31 2009-11-17 Dow Global Technologies Inc Bacterial leader sequences for increased expression
WO2008113041A2 (en) * 2007-03-14 2008-09-18 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
EP3369807A3 (en) 2007-03-28 2018-11-21 REG Life Sciences, LLC Enhanced production of fatty acid derivatives
CN101809146B (en) 2007-05-22 2013-12-11 Ls9公司 Hydrocarbon-producing genes and methods of their use
MY154965A (en) * 2007-06-01 2015-08-28 Solazyme Inc Production of oil in microorganisms
WO2009006430A1 (en) 2007-06-29 2009-01-08 The Regents Of The University Of California Host cells and methods for producing fatty acid derived compounds
US20090191599A1 (en) 2007-09-10 2009-07-30 Joule Biotechnologies, Inc. Engineered light-harvesting organisms
AU2008308477A1 (en) 2007-10-04 2009-04-09 Bio Architecture Lab, Inc. Biofuel production
CA2704227A1 (en) 2007-11-10 2009-05-14 Joule Biotechnologies, Inc. Hyperphotosynthetic organisms
US8445248B2 (en) * 2008-11-17 2013-05-21 Board Of Regents Of The Nevada System Of Higher Education On Behalf Of The University Of Nevada, Reno Hydrocarbon-forming oxidative decarbonylase enzyme, hydrocarbons produced thereby, and method of use
JP5859311B2 (en) * 2008-11-28 2016-02-10 ソラザイム, インコーポレイテッドSolazyme Inc Production of oil according to use in recombinant heterotrophic microorganisms
EP2373778A2 (en) 2008-12-11 2011-10-12 Joule Unlimited, Inc. Solar biofactory, photobioreactors, passive thermal regulation systems and methods for producing products
AU2009329970B2 (en) * 2008-12-23 2016-07-28 Genomatica, Inc. Methods and compositions related to thioesterase enzymes
US7955820B1 (en) * 2009-07-09 2011-06-07 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
US7794969B1 (en) * 2009-07-09 2010-09-14 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
HU231091B1 (en) * 2009-09-30 2020-07-28 Mol Magyar Olaj- És Gázipari Nyilvánosan Működő Részvénytársaság Fuels and fuel additives for combustion engines and method for producing them
WO2012037288A2 (en) 2010-09-14 2012-03-22 Joule Unlimited Technologies, Inc. Methods and compositions for the extracellular transport of biosynthetic hydrocarbons and other molecules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008003078A2 (en) * 2006-06-29 2008-01-03 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
WO2009111513A1 (en) * 2008-03-03 2009-09-11 Joule Biotechnologies, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
WO2009140695A1 (en) * 2008-05-16 2009-11-19 Ls9, Inc. Methods and compositions for producing hydrocarbons

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANDREAS SCHIRMER ET AL: "Microbial biosynthesis of alkanes", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 329, no. 5991, 30 July 2010 (2010-07-30), pages 559 - 562, XP002640301, ISSN: 1095-9203, DOI: 10.1126/SCIENCE.1187936 *
CHIEKO SUGITA ET AL: "Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization", PHOTOSYNTHESIS RESEARCH ; OFFICIAL JOURNAL OF THE INTERNATIONAL SOCIETY OF PHOTOSYNTHESIS RESEARCH, SPRINGER, BERLIN, DE, vol. 93, no. 1-3, 9 January 2007 (2007-01-09), pages 55 - 67, XP019532934, ISSN: 1573-5079, DOI: 10.1007/S11120-006-9122-4 *
HELLINGWERF K J ET AL: "Alternative routes to biofuels: Light-driven biofuel formation from CO2 and water based on the 'photanol' approach", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 142, no. 1, 1 June 2009 (2009-06-01), pages 87 - 90, XP026139441, ISSN: 0168-1656, [retrieved on 20090213], DOI: 10.1016/J.JBIOTEC.2009.02.002 *
LADYGINA N ET AL: "A review on microbial synthesis of hydrocarbons", PROCESS BIOCHEMISTRY, ELSEVIER, NL, vol. 41, no. 5, 1 May 2006 (2006-05-01), pages 1001 - 1014, XP002545855, ISSN: 1359-5113, DOI: 10.1016/J.PROCBIO.2005.12.007 *
NING LI ET AL: "Conversion of Fatty Aldehydes to Alka(e)nes and Formate by a Cyanobacterial Aldehyde Decarbonylase: Cryptic Redox by an Unusual Dimetal Oxygenase", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 133, no. 16, 27 April 2011 (2011-04-27), pages 6158 - 6161, XP055004891, ISSN: 0002-7863, DOI: 10.1021/ja2013517 *

Also Published As

Publication number Publication date
EP2787061A1 (en) 2014-10-08
US20110172467A1 (en) 2011-07-14
AU2010246473B2 (en) 2011-04-28
CN102597248B (en) 2014-12-31
EA201270151A1 (en) 2012-06-29
US9458069B2 (en) 2016-10-04
CN102597248A (en) 2012-07-18
EP2307553A2 (en) 2011-04-13
WO2011006137A2 (en) 2011-01-13
US20140005439A1 (en) 2014-01-02
US7919303B2 (en) 2011-04-05
EP2307553A4 (en) 2011-09-28
BR112012000355A2 (en) 2015-10-13
US7794969B1 (en) 2010-09-14
WO2011006137A3 (en) 2011-03-03
US20110009674A1 (en) 2011-01-13
CN104630279A (en) 2015-05-20
EP2584032A2 (en) 2013-04-24
EP2307553B1 (en) 2013-03-13
CA2766204A1 (en) 2011-01-13
MY156452A (en) 2016-02-26
ES2413484T3 (en) 2013-07-16
US20120095266A1 (en) 2012-04-19
ES2530752T3 (en) 2015-03-05
US20170051315A1 (en) 2017-02-23
EP2584032B1 (en) 2014-11-19
IL217389A0 (en) 2012-02-29
US8481285B2 (en) 2013-07-09
AU2010246473A1 (en) 2011-01-27
US8101397B2 (en) 2012-01-24
MX2012000448A (en) 2012-10-05

Similar Documents

Publication Publication Date Title
EP2584032A3 (en) Methods and compositions for the recombinant biosynthesis of n-alkanes
IN2012DN00462A (en)
MX2011004049A (en) Ethanol production by microorganisms.
PH12016501930A1 (en) Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods or making and uses thereof
WO2010054325A3 (en) Preservation and composition of bioprocess algae for production of lipids, seedstock, and feed
EP3312284A3 (en) Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene
MY158663A (en) Fermentation broth formulations
WO2012094425A3 (en) Biological methods for preparing adipic acid
MX2010009661A (en) Engineered co2 fixing microorganisms producing carbon-based products of interest.
WO2010129294A3 (en) Small molecules supporting pluripotent cell growth and methods thereof
WO2009002456A3 (en) Methods and compositions relating to progenitor cells
WO2009105733A9 (en) Designer organisms for photobiological butanol production from carbon dioxide and water
NZ594516A (en) Apolipoprotein a-i mimics
EP2599863A3 (en) Beta-glucosidase variants with improved properties
WO2009002772A3 (en) Bioreactor
WO2007134294A3 (en) Novel chlorella species and uses therefor
EP3050972A3 (en) Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
WO2010107914A3 (en) Biomass production and processing and methods of use thereof
EP2749652A3 (en) A method for producing a target substance by fermentation
WO2011022542A3 (en) Administration of factors normally present in a microbial niche to improve health
EA201101626A1 (en) BACTERIA WITH HIGHLY EFFICIENT METABOLISM
MX336787B (en) Genetically engineered microorganisms comprising 4-hydroxybenzoly-coa thioesterases and methods of using the same for producing free fatty acids and fatty acid derivatives.
WO2012018699A3 (en) Improved fermentation process for the production of organic acids
WO2012177983A3 (en) Microorganisms for producing ethylene glycol and methods related thereto
EP2392649A3 (en) Adipoyl-7-ADCA producing strains

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AC Divisional application: reference to earlier application

Ref document number: 2307553

Country of ref document: EP

Kind code of ref document: P

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 9/04 20060101ALI20130717BHEP

Ipc: C12N 1/21 20060101AFI20130717BHEP

Ipc: C12P 7/04 20060101ALI20130717BHEP

Ipc: C12N 9/02 20060101ALI20130717BHEP

Ipc: C12N 9/88 20060101ALI20130717BHEP

Ipc: C12P 5/02 20060101ALI20130717BHEP

17P Request for examination filed

Effective date: 20140131

RBV Designated contracting states (corrected)

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20140527

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AC Divisional application: reference to earlier application

Ref document number: 2307553

Country of ref document: EP

Kind code of ref document: P

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 697054

Country of ref document: AT

Kind code of ref document: T

Effective date: 20141215

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602010020472

Country of ref document: DE

Effective date: 20141231

REG Reference to a national code

Ref country code: NL

Ref legal event code: T3

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2530752

Country of ref document: ES

Kind code of ref document: T3

Effective date: 20150305

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 697054

Country of ref document: AT

Kind code of ref document: T

Effective date: 20141119

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150319

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150219

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150319

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150220

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602010020472

Country of ref document: DE

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: NL

Payment date: 20150726

Year of fee payment: 6

26N No opposition filed

Effective date: 20150820

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: ES

Payment date: 20150727

Year of fee payment: 6

Ref country code: FI

Payment date: 20150729

Year of fee payment: 6

Ref country code: GB

Payment date: 20150727

Year of fee payment: 6

Ref country code: DE

Payment date: 20150729

Year of fee payment: 6

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20150717

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150709

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150731

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150731

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150709

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 602010020472

Country of ref document: DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: MM

Effective date: 20160801

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20160709

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160801

Ref country code: NL

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160801

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170201

Ref country code: FI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160709

REG Reference to a national code

Ref country code: FR

Ref legal event code: ST

Effective date: 20170331

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20100709

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160709

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160710

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20141119

REG Reference to a national code

Ref country code: ES

Ref legal event code: FD2A

Effective date: 20181119