ES2530273T3 - Amplificación de ácidos nucleicos específica de alelo - Google Patents
Amplificación de ácidos nucleicos específica de alelo Download PDFInfo
- Publication number
- ES2530273T3 ES2530273T3 ES10795240.0T ES10795240T ES2530273T3 ES 2530273 T3 ES2530273 T3 ES 2530273T3 ES 10795240 T ES10795240 T ES 10795240T ES 2530273 T3 ES2530273 T3 ES 2530273T3
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- oligonucleotide
- target sequence
- nucleotide
- variant
- complementary
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
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| KR20140064971A (ko) | 2011-09-19 | 2014-05-28 | 제넨테크, 인크. | c-met 길항제 및 B-raf 길항제를 포함하는 조합 치료 |
| US9738935B2 (en) * | 2011-11-10 | 2017-08-22 | Roche Molecular Systems, Inc. | Complex mutations in the epidermal growth factor receptor kinase domain |
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| US9382581B2 (en) | 2012-12-13 | 2016-07-05 | Roche Molecular Systems, Inc. | Primers with modified phosphate and base in allele-specific PCR |
| CN105229171A (zh) * | 2013-03-13 | 2016-01-06 | 豪夫迈·罗氏有限公司 | 在人pi3kca (pik3ca)基因中检测突变的方法和组合物 |
| CN103911445B (zh) * | 2014-03-20 | 2015-08-12 | 上海科亦生物科技有限公司 | 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒 |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| KR101756875B1 (ko) * | 2015-05-28 | 2017-07-11 | 에스디 바이오센서 주식회사 | 상보적 염기서열과 미스-매치된 염기서열 그리고 reporter와 quencher를 포함하는 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭 및 측정 방법 |
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| CN116949140A (zh) * | 2016-02-17 | 2023-10-27 | 哈佛学院院长及董事 | 分子编程工具 |
| JP2018164421A (ja) * | 2017-03-28 | 2018-10-25 | 東洋紡株式会社 | 核酸増幅方法 |
| CN111868260B (zh) | 2017-08-07 | 2025-02-21 | 约翰斯霍普金斯大学 | 用于评估和治疗癌症的方法和材料 |
| WO2019099420A1 (en) * | 2017-11-15 | 2019-05-23 | Yan Wang | A method for detecting multiple dna mutations and copy number variations |
| CN115516108A (zh) | 2020-02-14 | 2022-12-23 | 约翰斯霍普金斯大学 | 评估核酸的方法和材料 |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DE3529478A1 (de) | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung |
| US5795762A (en) | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US5521301A (en) | 1988-12-12 | 1996-05-28 | City Of Hope | Genotyping of multiple allele systems |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| ES2141088T3 (es) | 1990-02-16 | 2000-03-16 | Hoffmann La Roche | Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa. |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| JPH04234422A (ja) | 1990-10-31 | 1992-08-24 | Internatl Business Mach Corp <Ibm> | 二重硬化エポキシバックシール処方物 |
| US5840867A (en) | 1991-02-21 | 1998-11-24 | Gilead Sciences, Inc. | Aptamer analogs specific for biomolecules |
| US5644048A (en) | 1992-01-10 | 1997-07-01 | Isis Pharmaceuticals, Inc. | Process for preparing phosphorothioate oligonucleotides |
| DE4234086A1 (de) | 1992-02-05 | 1993-08-12 | Diagen Inst Molekularbio | Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| EP0866071B1 (en) * | 1997-03-20 | 2004-10-20 | F. Hoffmann-La Roche Ag | Modified primers |
| ATE236997T1 (de) | 1998-07-21 | 2003-04-15 | Keygene Nv | Verbesserte primer zur aflp amplifizierung |
| US6200757B1 (en) | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
| EP1088891B1 (en) * | 1999-09-28 | 2005-01-12 | Roche Diagnostics GmbH | Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro |
| EP1241266A4 (en) | 1999-12-10 | 2004-11-17 | Toyo Boseki | METHOD FOR DETECTING NUCLEIC ACID POLYMORPHISMS |
| US7582420B2 (en) * | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| CN100351393C (zh) | 2001-02-15 | 2007-11-28 | 宝生物工程株式会社 | 核苷酸多态性的检测方法 |
| WO2003072814A2 (en) | 2002-02-26 | 2003-09-04 | Roche Diagnostics Gmbh | Improved method for allele-specific pcr |
| US7148049B2 (en) | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
| US20050019918A1 (en) * | 2003-06-03 | 2005-01-27 | Hidetoshi Sumimoto | Treatment of cancer by inhibiting BRAF expression |
| US7408051B2 (en) | 2004-04-14 | 2008-08-05 | Applera Corporation | Modified oligonucleotides and applications thereof |
| WO2006047481A2 (en) * | 2004-10-22 | 2006-05-04 | Redpath Integrated Pathology, Inc. | Enhanced amplifiability of minute fixative-treated tissue samples, minute stained cytology samples, and other minute sources of dna |
| US7442507B2 (en) | 2005-01-24 | 2008-10-28 | New York University School Of Medicine | Methods for detecting circulating mutant BRAF DNA |
| JP3859687B1 (ja) | 2005-11-21 | 2006-12-20 | 松下電器産業株式会社 | Ｄｎａが有する標的塩基を判別する方法 |
| WO2007127992A2 (en) | 2006-04-28 | 2007-11-08 | Igor Kutyavin | Use of base-modified deoxynucleoside triphosphates |
| JP2009077712A (ja) * | 2007-09-11 | 2009-04-16 | F Hoffmann La Roche Ag | Ｂ−Ｒａｆキナーゼ阻害剤に対する感受性についての診断試験 |
| EP2337865B1 (en) | 2008-10-20 | 2014-11-19 | Roche Diagnostics GmbH | Allele-specific amplification using a primer with a modified nucleotide |
| EP2418515A1 (en) | 2010-07-21 | 2012-02-15 | Astrium GmbH | Integrity method for differential corrections |
-
2010
- 2010-12-08 US US12/962,861 patent/US9238832B2/en active Active
- 2010-12-10 ES ES10795240.0T patent/ES2530273T3/es active Active
- 2010-12-10 JP JP2012542402A patent/JP5886755B2/ja active Active
- 2010-12-10 CA CA2781984A patent/CA2781984C/en active Active
- 2010-12-10 CN CN201710169202.2A patent/CN106987624A/zh active Pending
- 2010-12-10 CN CN201080055770.6A patent/CN102656278A/zh active Pending
- 2010-12-10 EP EP10795240.0A patent/EP2510117B1/en active Active
- 2010-12-10 WO PCT/EP2010/007560 patent/WO2011069677A1/en not_active Ceased
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2015
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Also Published As
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|---|---|
| US20110311968A1 (en) | 2011-12-22 |
| WO2011069677A1 (en) | 2011-06-16 |
| EP2510117A1 (en) | 2012-10-17 |
| CN106987624A (zh) | 2017-07-28 |
| CA2781984A1 (en) | 2011-06-16 |
| US9238832B2 (en) | 2016-01-19 |
| CA2781984C (en) | 2015-02-17 |
| JP2013513360A (ja) | 2013-04-22 |
| JP2016013137A (ja) | 2016-01-28 |
| JP5886755B2 (ja) | 2016-03-16 |
| EP2510117B1 (en) | 2014-12-10 |
| CN102656278A (zh) | 2012-09-05 |
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