ES2469816T3 - Aislamiento de ácido nucleico - Google Patents

Aislamiento de ácido nucleico Download PDF

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Publication number
ES2469816T3
ES2469816T3 ES09784897.2T ES09784897T ES2469816T3 ES 2469816 T3 ES2469816 T3 ES 2469816T3 ES 09784897 T ES09784897 T ES 09784897T ES 2469816 T3 ES2469816 T3 ES 2469816T3
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ES
Spain
Prior art keywords
buffer
nucleic acid
solid phase
binding
wash
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09784897.2T
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English (en)
Spanish (es)
Inventor
Yii Leng Chua
Allyson Victoria Ritchie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambridge Enterprise Ltd
Original Assignee
Cambridge Enterprise Ltd
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39790526&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2469816(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cambridge Enterprise Ltd filed Critical Cambridge Enterprise Ltd
Application granted granted Critical
Publication of ES2469816T3 publication Critical patent/ES2469816T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ES09784897.2T 2008-08-08 2009-08-07 Aislamiento de ácido nucleico Active ES2469816T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0814570 2008-08-08
GBGB0814570.8A GB0814570D0 (en) 2008-08-08 2008-08-08 Isolation of nucleic acid
PCT/GB2009/001951 WO2010015835A1 (en) 2008-08-08 2009-08-07 Isolation of nucleic acid

Publications (1)

Publication Number Publication Date
ES2469816T3 true ES2469816T3 (es) 2014-06-20

Family

ID=39790526

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09784897.2T Active ES2469816T3 (es) 2008-08-08 2009-08-07 Aislamiento de ácido nucleico

Country Status (7)

Country Link
US (2) US9422543B2 (enExample)
EP (1) EP2329019B1 (enExample)
JP (3) JP6125752B2 (enExample)
AU (2) AU2009278915B2 (enExample)
ES (1) ES2469816T3 (enExample)
GB (1) GB0814570D0 (enExample)
WO (1) WO2010015835A1 (enExample)

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GB201304797D0 (en) 2013-03-15 2013-05-01 Diagnostics For The Real World Ltd Apparatus and method for automated sample preparation and adaptor for use in the apparatus
CA2967008A1 (en) * 2014-11-07 2016-05-12 The Johns Hopkins University Chaotrope- and volatile-free method for purifying nucleic acids from plasma
EP3334825A4 (en) * 2015-08-11 2019-03-13 Stem Arts Projects, LLC PORTABLE NUCLEIC ACID EXTRACTION DEVICE AND METHOD OF USE THEREOF
US11427815B2 (en) 2015-12-28 2022-08-30 Koninklijke Philips N.V. Nucleic acid purification system using a single wash and elution buffer solution
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
US20200063189A1 (en) * 2017-02-27 2020-02-27 miDiagnostics NV System and method for purifying and amplifying nucleic acids
US11149265B2 (en) 2017-09-14 2021-10-19 California Institute Of Technology Purification and detection of analytes
CN112105927B (zh) * 2018-05-08 2024-06-25 沃特世科技公司 可用于pH梯度阳离子交换色谱法的方法、组合物和试剂盒
GB201819726D0 (en) * 2018-12-03 2019-01-16 Diagnostics For The Real World Ltd HCV detection
US12565644B2 (en) 2019-08-08 2026-03-03 Biocartis Nv Nucleic acid purification chemistry
US11866695B2 (en) 2019-12-23 2024-01-09 California Institute Of Technology Methods and systems and related compositions for mixtures separation with a solid matrix
GB202003860D0 (en) 2020-03-17 2020-04-29 Diagnostics For The Real World Ltd Sample collection device
EP4127246B1 (en) 2020-03-23 2026-04-29 Diagnostics for the Real World, Ltd Coronavirus detection
EP4159855A4 (en) * 2020-05-25 2024-07-24 Sekisui Medical Co., Ltd. METHOD FOR PURIFICATION OF NUCLEIC ACID
JP2025507270A (ja) * 2022-02-05 2025-03-18 ベクトン・ディキンソン・アンド・カンパニー 短い核酸標的の増幅のためにゲノムｄｎａを分離するための方法
CN114317527A (zh) * 2022-02-18 2022-04-12 欧蒙医学诊断（中国）有限公司 用于从样本中提取核酸的方法和试剂盒

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US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
US6090591A (en) 1987-07-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
IE72468B1 (en) 1987-07-31 1997-04-09 Univ Leland Stanford Junior Selective amplification of target polynucleotide sequences
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
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CA2067711C (en) * 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
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US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
GB9725839D0 (en) 1997-12-06 1998-02-04 Baker Matthew J Isolation of nucleic acids
WO1999040098A1 (de) 1998-02-04 1999-08-12 Merck Patent Gmbh Verfahren zur isolierung und aufreinigung von nucleinsäuren
US6203989B1 (en) 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
DE19851156C1 (de) 1998-11-06 2000-04-27 Merck Patent Gmbh Verfahren zur Isolierung von Plasmid-DNA
DE19856064C2 (de) * 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6803196B1 (en) 2000-10-13 2004-10-12 Affymetrix, Inc. Methods and compositions for detecting signals in binding assays using microparticles
EP1560926B2 (de) * 2002-11-08 2013-08-21 STRATEC Molecular GmbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
DE10258258A1 (de) * 2002-12-13 2004-06-24 Merck Patent Gmbh Verfahren zur Aufreinigung von Nukleinsäuren
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DE102004063265B4 (de) * 2004-12-29 2012-02-02 Biontech Ag Verfahren zur Bestimmung der Funktion von Nukleinsäuresequenzen und den dadurch kodierten Expressionsprodukten
US20070184472A1 (en) * 2005-06-08 2007-08-09 Toagosei Co., Ltd Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna
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JP5268963B2 (ja) 2010-02-10 2013-08-21 アズビル株式会社 圧力測定システム及び圧力測定方法

Also Published As

Publication number Publication date
JP2011530278A (ja) 2011-12-22
JP6571544B2 (ja) 2019-09-04
US20110257386A1 (en) 2011-10-20
WO2010015835A1 (en) 2010-02-11
JP2016082980A (ja) 2016-05-19
JP6125752B2 (ja) 2017-05-10
AU2016203697A1 (en) 2016-06-23
AU2009278915A1 (en) 2010-02-11
US20170037394A1 (en) 2017-02-09
GB0814570D0 (en) 2008-09-17
EP2329019A1 (en) 2011-06-08
US9422543B2 (en) 2016-08-23
AU2009278915B2 (en) 2016-03-03
EP2329019B1 (en) 2014-03-12
JP2018042565A (ja) 2018-03-22

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