AU2009278915B2 - Isolation of nucleic acid - Google Patents

Isolation of nucleic acid Download PDF

Info

Publication number
AU2009278915B2
AU2009278915B2 AU2009278915A AU2009278915A AU2009278915B2 AU 2009278915 B2 AU2009278915 B2 AU 2009278915B2 AU 2009278915 A AU2009278915 A AU 2009278915A AU 2009278915 A AU2009278915 A AU 2009278915A AU 2009278915 B2 AU2009278915 B2 AU 2009278915B2
Authority
AU
Australia
Prior art keywords
nucleic acid
buffer
solid phase
wash solution
buffering range
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2009278915A
Other languages
English (en)
Other versions
AU2009278915A1 (en
Inventor
Yii Leng Chua
Allyson Victoria Ritchie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambridge Enterprise Ltd
Original Assignee
Cambridge Enterprise Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39790526&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2009278915(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cambridge Enterprise Ltd filed Critical Cambridge Enterprise Ltd
Publication of AU2009278915A1 publication Critical patent/AU2009278915A1/en
Application granted granted Critical
Publication of AU2009278915B2 publication Critical patent/AU2009278915B2/en
Priority to AU2016203697A priority Critical patent/AU2016203697A1/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2009278915A 2008-08-08 2009-08-07 Isolation of nucleic acid Ceased AU2009278915B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2016203697A AU2016203697A1 (en) 2008-08-08 2016-06-03 Isolation of nucleic acid

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0814570.8 2008-08-08
GBGB0814570.8A GB0814570D0 (en) 2008-08-08 2008-08-08 Isolation of nucleic acid
PCT/GB2009/001951 WO2010015835A1 (en) 2008-08-08 2009-08-07 Isolation of nucleic acid

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2016203697A Division AU2016203697A1 (en) 2008-08-08 2016-06-03 Isolation of nucleic acid

Publications (2)

Publication Number Publication Date
AU2009278915A1 AU2009278915A1 (en) 2010-02-11
AU2009278915B2 true AU2009278915B2 (en) 2016-03-03

Family

ID=39790526

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2009278915A Ceased AU2009278915B2 (en) 2008-08-08 2009-08-07 Isolation of nucleic acid
AU2016203697A Abandoned AU2016203697A1 (en) 2008-08-08 2016-06-03 Isolation of nucleic acid

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2016203697A Abandoned AU2016203697A1 (en) 2008-08-08 2016-06-03 Isolation of nucleic acid

Country Status (7)

Country Link
US (2) US9422543B2 (enExample)
EP (1) EP2329019B1 (enExample)
JP (3) JP6125752B2 (enExample)
AU (2) AU2009278915B2 (enExample)
ES (1) ES2469816T3 (enExample)
GB (1) GB0814570D0 (enExample)
WO (1) WO2010015835A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013035062A1 (en) * 2011-09-06 2013-03-14 North-West University A method of preparing biological material
GB2501238B (en) * 2012-03-16 2018-10-24 Cambridge Entpr Ltd Methods for obtaining liquid from a solid phase
CN104350152B (zh) 2012-06-01 2017-08-11 欧米伽生物技术公司 选择性核酸片段回收
GB201304797D0 (en) 2013-03-15 2013-05-01 Diagnostics For The Real World Ltd Apparatus and method for automated sample preparation and adaptor for use in the apparatus
CA2967008A1 (en) * 2014-11-07 2016-05-12 The Johns Hopkins University Chaotrope- and volatile-free method for purifying nucleic acids from plasma
EP3334825A4 (en) * 2015-08-11 2019-03-13 Stem Arts Projects, LLC PORTABLE NUCLEIC ACID EXTRACTION DEVICE AND METHOD OF USE THEREOF
US11427815B2 (en) 2015-12-28 2022-08-30 Koninklijke Philips N.V. Nucleic acid purification system using a single wash and elution buffer solution
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
US20200063189A1 (en) * 2017-02-27 2020-02-27 miDiagnostics NV System and method for purifying and amplifying nucleic acids
US11149265B2 (en) 2017-09-14 2021-10-19 California Institute Of Technology Purification and detection of analytes
CN112105927B (zh) * 2018-05-08 2024-06-25 沃特世科技公司 可用于pH梯度阳离子交换色谱法的方法、组合物和试剂盒
GB201819726D0 (en) * 2018-12-03 2019-01-16 Diagnostics For The Real World Ltd HCV detection
US12565644B2 (en) 2019-08-08 2026-03-03 Biocartis Nv Nucleic acid purification chemistry
US11866695B2 (en) 2019-12-23 2024-01-09 California Institute Of Technology Methods and systems and related compositions for mixtures separation with a solid matrix
GB202003860D0 (en) 2020-03-17 2020-04-29 Diagnostics For The Real World Ltd Sample collection device
EP4127246B1 (en) 2020-03-23 2026-04-29 Diagnostics for the Real World, Ltd Coronavirus detection
EP4159855A4 (en) * 2020-05-25 2024-07-24 Sekisui Medical Co., Ltd. METHOD FOR PURIFICATION OF NUCLEIC ACID
JP2025507270A (ja) * 2022-02-05 2025-03-18 ベクトン・ディキンソン・アンド・カンパニー 短い核酸標的の増幅のためにゲノムdnaを分離するための方法
CN114317527A (zh) * 2022-02-18 2022-04-12 欧蒙医学诊断(中国)有限公司 用于从样本中提取核酸的方法和试剂盒

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000029562A1 (de) * 1998-11-06 2000-05-25 Merck Patent Gmbh Verfahren zur isolierung von plasmid-dna
US6355792B1 (en) * 1998-02-04 2002-03-12 Merck Patent Gesellschaft Method for isolating and purifying nucleic acids
WO2004055207A1 (de) * 2002-12-13 2004-07-01 Merck Patent Gmbh Verfahren zur aufreinigung von nukleinsäuren

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5955261A (en) 1984-09-04 1999-09-21 Gen-Probe Incorporated Method for detecting the presence of group-specific viral mRNA in a sample
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
US6090591A (en) 1987-07-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
IE72468B1 (en) 1987-07-31 1997-04-09 Univ Leland Stanford Junior Selective amplification of target polynucleotide sequences
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
AU2684488A (en) 1988-06-27 1990-01-04 Carter-Wallace, Inc. Test device and method for colored particle immunoassay
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
CA2067711C (en) * 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
WO1994002634A1 (en) 1992-07-24 1994-02-03 University Of South Australia Amplification and detection process
ZA936016B (en) 1992-08-24 1994-03-10 Akzo Nv Method for nucleic acid amplification
JP4108118B2 (ja) 1993-03-26 2008-06-25 ジェン−プローブ・インコーポレイテッド ヒト・免疫不全ウイルス1型の検出
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
JP3082908B2 (ja) * 1996-07-12 2000-09-04 東洋紡績株式会社 リボ核酸の単離方法
DE69734263T2 (de) 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
US5981254A (en) * 1997-10-30 1999-11-09 Haemacure Corporation Process for producing thrombin from plasma
ES2177093T3 (es) * 1997-12-06 2002-12-01 Dna Res Innovations Ltd Aislamiento de acidos nucleicos.
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
GB9725839D0 (en) 1997-12-06 1998-02-04 Baker Matthew J Isolation of nucleic acids
US6203989B1 (en) 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
DE19856064C2 (de) * 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6803196B1 (en) 2000-10-13 2004-10-12 Affymetrix, Inc. Methods and compositions for detecting signals in binding assays using microparticles
EP1560926B2 (de) * 2002-11-08 2013-08-21 STRATEC Molecular GmbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
EP1603674B1 (en) 2003-02-05 2016-01-06 Iquum, Inc. Sample processing
EP1498133A1 (en) * 2003-07-18 2005-01-19 Aventis Pharma Deutschland GmbH Use of a pak inhibitor for the treatment of a joint disease
EP1529840A1 (en) * 2003-11-04 2005-05-11 Qiagen GmbH A rapid and low cost method for isolating nucleic acid
AU2005323451B2 (en) 2004-05-21 2010-11-18 Qiagen Sciences Llc Kits and processes for removing contaminants from nucleic acids in environmental and biological samples
DE102004063265B4 (de) * 2004-12-29 2012-02-02 Biontech Ag Verfahren zur Bestimmung der Funktion von Nukleinsäuresequenzen und den dadurch kodierten Expressionsprodukten
US20070184472A1 (en) * 2005-06-08 2007-08-09 Toagosei Co., Ltd Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna
CA2631829C (en) * 2005-12-14 2012-01-24 F. Hoffmann-La Roche Ag New method for bisulfite treatment
JP2007244375A (ja) * 2006-02-14 2007-09-27 Toyobo Co Ltd リボ核酸の分離精製方法
WO2008035991A2 (en) 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method
JP5268963B2 (ja) 2010-02-10 2013-08-21 アズビル株式会社 圧力測定システム及び圧力測定方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6355792B1 (en) * 1998-02-04 2002-03-12 Merck Patent Gesellschaft Method for isolating and purifying nucleic acids
WO2000029562A1 (de) * 1998-11-06 2000-05-25 Merck Patent Gmbh Verfahren zur isolierung von plasmid-dna
WO2004055207A1 (de) * 2002-12-13 2004-07-01 Merck Patent Gmbh Verfahren zur aufreinigung von nukleinsäuren

Also Published As

Publication number Publication date
JP2011530278A (ja) 2011-12-22
JP6571544B2 (ja) 2019-09-04
US20110257386A1 (en) 2011-10-20
ES2469816T3 (es) 2014-06-20
WO2010015835A1 (en) 2010-02-11
JP2016082980A (ja) 2016-05-19
JP6125752B2 (ja) 2017-05-10
AU2016203697A1 (en) 2016-06-23
AU2009278915A1 (en) 2010-02-11
US20170037394A1 (en) 2017-02-09
GB0814570D0 (en) 2008-09-17
EP2329019A1 (en) 2011-06-08
US9422543B2 (en) 2016-08-23
EP2329019B1 (en) 2014-03-12
JP2018042565A (ja) 2018-03-22

Similar Documents

Publication Publication Date Title
EP2329019B1 (en) Isolation of nucleic acid
US10947527B2 (en) Compositions and methods for purifying nucleic acids from stabilization reagents
US10273470B2 (en) Method for isolating RNA from a RNA and DNA containing sample
EP1983051B1 (en) Isolation and purification of nucleic acid molecules with solid phase
EP2094846B1 (en) Use of tde for the isolation of nucleic acids
KR20030009361A (ko) 핵산 분석용 용기
US20130041145A1 (en) Method for isolating rna from whole blood samples
EP1529840A1 (en) A rapid and low cost method for isolating nucleic acid
CA2728232A1 (en) Cell lysis reagent for isolation of rna
CN111206073B (zh) 一种硅珠法核酸提取试剂盒及其使用方法和应用
US20090176296A1 (en) Process for isolating nucleic acids
US10131935B2 (en) Method for parallel isolation of viral nucleic acids
WO2009040444A1 (en) Rna isolation method
CN119020459A (zh) 适用于多样本快速释放的核酸释放剂及核酸释放方法

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired