ES2461896B1 - Métodos para obtener plantas resistentes a sequía - Google Patents
Métodos para obtener plantas resistentes a sequía Download PDFInfo
- Publication number
- ES2461896B1 ES2461896B1 ES201350005A ES201350005A ES2461896B1 ES 2461896 B1 ES2461896 B1 ES 2461896B1 ES 201350005 A ES201350005 A ES 201350005A ES 201350005 A ES201350005 A ES 201350005A ES 2461896 B1 ES2461896 B1 ES 2461896B1
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- transformed cells
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- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- 230000000426 osmoregulatory effect Effects 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 abstract 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 abstract 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 231100000252 nontoxic Toxicity 0.000 abstract 1
- 230000003000 nontoxic effect Effects 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención provee un método no tóxico para la selección de células transformadas de entre una población que consiste de células transformadas y no transformadas. El método comprende los siguientes pasos: a) introducir a una célula al menos una secuencia nucleotídica de interés y al menos una secuencia nucleotídica de selección para obtener células genéticamente transformadas, donde la secuencia nucleotídica de selección comprende una secuencia que promueve la inhibición de la enzima trehalasa endógena; b) colocar la población con células transformadas y no transformadas en un medio de cultivo que contiene una sustancia osmoreguladora como el PEG 8000 y c) seleccionar las células transformadas de la población en base a la capacidad de las células transformadas de sobrevivir y desarrollarse en presencia de la sustancia osmoreguladora, confiriendo a las plantas derivadas de estos eventos de transformación genética, por ejemplo de capacidad de tolerancia a sequía y frío.
Description
Claims (1)
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imagen1 imagen2 imagen3 imagen4 imagen5 imagen6 imagen7 imagen8 imagen9 imagen10 imagen11 imagen12
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2010014428A MX366670B (es) | 2010-12-21 | 2010-12-21 | Método para la selección positiva de células vegetales genéticamente transformadas de maíz y otras especies. |
| MX2010014428 | 2010-12-21 | ||
| MX2011013595A MX350652B (es) | 2011-12-15 | 2011-12-15 | Metodos para obtener plantas resistentes a sequia. |
| MX2011013595 | 2011-12-15 | ||
| PCT/IB2011/055757 WO2012085806A1 (en) | 2010-12-21 | 2011-12-17 | Methods to obtain drought resistant plants |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ES2461896A2 ES2461896A2 (es) | 2014-05-21 |
| ES2461896R1 ES2461896R1 (es) | 2014-06-02 |
| ES2461896B1 true ES2461896B1 (es) | 2015-03-18 |
Family
ID=45607776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES201350005A Active ES2461896B1 (es) | 2010-12-21 | 2011-12-17 | Métodos para obtener plantas resistentes a sequía |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN103403168A (es) |
| ES (1) | ES2461896B1 (es) |
| WO (1) | WO2012085806A1 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103397097B (zh) * | 2013-08-07 | 2014-12-17 | 新疆农垦科学院 | 与小麦抗旱性相关的分子标记及其应用 |
| CN105441474B (zh) * | 2014-08-06 | 2019-01-15 | 中国科学院微生物研究所 | 一种受ABA调控的miRNA在提高植物抗旱性中的应用 |
| CN105660213A (zh) * | 2015-08-27 | 2016-06-15 | 安徽科技学院 | 一种利用peg-6000鉴定大豆发芽期耐旱性的方法 |
| RU2758117C2 (ru) | 2017-02-07 | 2021-10-26 | Юниверсити Оф Кентукки Рисёрч Фаундейшн | Способ увеличения содержания сложного эфира сахарозы в растениях табака |
| CN113151236B (zh) * | 2021-04-20 | 2022-06-28 | 华中农业大学 | 玉米抗旱基因ZmcPGM2及其应用 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6174724B1 (en) | 1983-01-17 | 2001-01-16 | Monsanto Company | Chimeric genes suitable for expression in plant cells |
| JPH0714349B2 (ja) | 1983-01-17 | 1995-02-22 | モンサント カンパニ− | 植物細胞での発現に適したキメラ遺伝子 |
| US4727027A (en) | 1983-05-02 | 1988-02-23 | Diamond Scientific Co. | Photochemical decontamination treatment of whole blood or blood components |
| GB9304200D0 (en) | 1993-03-02 | 1993-04-21 | Sandoz Ltd | Improvements in or relating to organic compounds |
| IL116564A0 (en) * | 1995-01-04 | 1996-03-31 | Mogen Int | Process for producing trehalose in plants |
| EP0784095A3 (en) * | 1996-01-12 | 1997-12-29 | Mogen International N.V. | Enhanced accummulation of trehalose in plants |
| GB9702592D0 (en) | 1997-02-07 | 1997-03-26 | Danisco | Selection method |
| US7214858B2 (en) | 1999-03-31 | 2007-05-08 | E. I. Du Pont De Nemours And Company | Plant genes encoding trehalose metabolism enzymes |
| US20020137214A1 (en) * | 2001-04-18 | 2002-09-26 | Henry Daniell | Marker free transgenic plants engineering the chloroplast genome without the use of antibiotic selection |
| NL1019308C2 (nl) * | 2001-11-06 | 2003-05-07 | Stichting Tech Wetenschapp | Werkwijze voor het selecteren van genetisch getransformeerde cellen. |
| US6939676B2 (en) * | 2002-12-30 | 2005-09-06 | The United States Of America As Represented By The Secretary Of Argriculture | Selection procedure for identifying transgenic cells, embryos, and plants without the use of antibiotics |
| CN1869240A (zh) * | 2006-05-26 | 2006-11-29 | 上海大学 | 一种提高植物耐盐性能的方法 |
| US7994390B2 (en) * | 2006-09-29 | 2011-08-09 | The Chinese University Of Hong Kong | Use of GmRD22-like genes to protect against abiotic stress |
| AP2010005368A0 (en) * | 2008-02-01 | 2010-08-31 | Temasek Life Sciences Lab Ltd | Regeneration and mass propagation of jatropha curcas through somatic embryogenesis |
-
2011
- 2011-12-17 ES ES201350005A patent/ES2461896B1/es active Active
- 2011-12-17 WO PCT/IB2011/055757 patent/WO2012085806A1/en active Application Filing
- 2011-12-17 CN CN2011800650019A patent/CN103403168A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ES2461896R1 (es) | 2014-06-02 |
| CN103403168A (zh) | 2013-11-20 |
| WO2012085806A1 (en) | 2012-06-28 |
| ES2461896A2 (es) | 2014-05-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NE2A | Request for restoration |
Effective date: 20131212 |
|
| FG2A | Definitive protection |
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