ES2351916B8 - Metodo de identificacion de celulas madre mesenquimales senescentes - Google Patents

Metodo de identificacion de celulas madre mesenquimales senescentes Download PDF

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Publication number
ES2351916B8
ES2351916B8 ES200930540A ES200930540A ES2351916B8 ES 2351916 B8 ES2351916 B8 ES 2351916B8 ES 200930540 A ES200930540 A ES 200930540A ES 200930540 A ES200930540 A ES 200930540A ES 2351916 B8 ES2351916 B8 ES 2351916B8
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Prior art keywords
senescent
identification
mesenchymal stem
stem cells
cells
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ES200930540A
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ES2351916B1 (es
ES2351916A1 (es
Inventor
Enrique Samper Rodriguez
Juan Camilo Estrada Rodriguez
Antonio Bernad Miana
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CENTRO NACIONAL DE INVESTIGACIONES CARDIOVASCULARES (CNIC)
CT NAC DE INVESTIGACIONES CARDIOVASCULARES CNIC
Original Assignee
CENTRO NACIONAL DE INVESTIGACIONES CARDIOVASCULARES (CNIC)
CT NAC DE INVESTIGACIONES CARDIOVASCULARES CNIC
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Priority to ES200930540A priority Critical patent/ES2351916B8/es
Priority to PCT/ES2010/070534 priority patent/WO2011012764A2/es
Priority to ES10803953.8T priority patent/ES2445157T3/es
Priority to EP10803953.8A priority patent/EP2460876B1/en
Priority to US13/387,694 priority patent/US20120165216A1/en
Publication of ES2351916A1 publication Critical patent/ES2351916A1/es
Publication of ES2351916B1 publication Critical patent/ES2351916B1/es
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0666—Mesenchymal stem cells from hair follicles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C12N5/0675—
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G06F19/20—
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Rheumatology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método de identificación de células madre mesenquimales senescentes.#La invención se refiere a un método para identificar células madre mesenquimales senescentes creciendo en cultivos in vitro, que comprende: medir la longitud de los telómeros de los cromosomas, determinar el nivel de ploidía en la célula, analizar la presencia de mitosis multipolares y analizar el nivel de expresión de los genes SCIN, AKAP9, EDN-1, CXCL1, CXCL12 y/o CD70. Este método puede ser de utilidad para la realización de estudios de estabilidad genética en los cultivos de células madre mesenquimales cuyas células van a ser empleadas en terapia celular, lo que permite identificar y seleccionar aquellos más estables y apropiados para tal fin.
ES200930540A 2009-07-30 2009-07-30 Metodo de identificacion de celulas madre mesenquimales senescentes Active ES2351916B8 (es)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ES200930540A ES2351916B8 (es) 2009-07-30 2009-07-30 Metodo de identificacion de celulas madre mesenquimales senescentes
PCT/ES2010/070534 WO2011012764A2 (es) 2009-07-30 2010-07-30 Método de identificación de células madre mesenquimales senescentes
ES10803953.8T ES2445157T3 (es) 2009-07-30 2010-07-30 Método de identificación de células madre mesenquimales senescentes
EP10803953.8A EP2460876B1 (en) 2009-07-30 2010-07-30 Method for identifying senescent mesenchymal stem cells
US13/387,694 US20120165216A1 (en) 2009-07-30 2010-07-30 Method for identifying senescent mesenchymal stem cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200930540A ES2351916B8 (es) 2009-07-30 2009-07-30 Metodo de identificacion de celulas madre mesenquimales senescentes

Publications (3)

Publication Number Publication Date
ES2351916A1 ES2351916A1 (es) 2011-02-14
ES2351916B1 ES2351916B1 (es) 2011-12-29
ES2351916B8 true ES2351916B8 (es) 2012-07-09

Family

ID=43528444

Family Applications (2)

Application Number Title Priority Date Filing Date
ES200930540A Active ES2351916B8 (es) 2009-07-30 2009-07-30 Metodo de identificacion de celulas madre mesenquimales senescentes
ES10803953.8T Active ES2445157T3 (es) 2009-07-30 2010-07-30 Método de identificación de células madre mesenquimales senescentes

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES10803953.8T Active ES2445157T3 (es) 2009-07-30 2010-07-30 Método de identificación de células madre mesenquimales senescentes

Country Status (4)

Country Link
US (1) US20120165216A1 (es)
EP (1) EP2460876B1 (es)
ES (2) ES2351916B8 (es)
WO (1) WO2011012764A2 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2016138847A (ru) * 2014-04-07 2018-05-07 Хэлф Ресерч, Инк. Композиции и способы, относящиеся к покоящимся предрасположенным к старению клеткам (DSPC)
EP3091084A1 (en) * 2015-05-08 2016-11-09 Université Catholique De Louvain Methods for assessing the purity of a mesenchymal stem cells preparation
US11291689B2 (en) 2015-06-03 2022-04-05 Aelan Cell Technologies, Inc. Methods and devices for the production and delivery of beneficial factors from adipose-derived stem cells
US20180136209A1 (en) * 2015-06-03 2018-05-17 Aelan Cell Technologies, Inc. Companion methods and kits for il-2-based therapies and mesenchymal stem cell-based therapies
CN114574570A (zh) * 2022-04-08 2022-06-03 青岛啤酒股份有限公司 一种啤酒酵母高代数使用的评估方法及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6566399B2 (en) 2000-08-03 2003-05-20 Byung-Moo Min Inhibitor of replicative senescence of human keratinocytes containing retinoic acid as active ingredients
WO2009073518A1 (en) * 2007-11-30 2009-06-11 New York Medical College Methods of isolating non-senescent cardiac stem cells and uses thereof

Also Published As

Publication number Publication date
US20120165216A1 (en) 2012-06-28
WO2011012764A2 (es) 2011-02-03
EP2460876B1 (en) 2013-09-04
EP2460876A4 (en) 2012-12-26
ES2351916B1 (es) 2011-12-29
ES2351916A1 (es) 2011-02-14
WO2011012764A3 (es) 2011-07-14
EP2460876A2 (en) 2012-06-06
ES2445157T3 (es) 2014-02-28

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