WO2011012764A3 - Método de identificación de células madre mesenquimales senescentes - Google Patents

Método de identificación de células madre mesenquimales senescentes Download PDF

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Publication number
WO2011012764A3
WO2011012764A3 PCT/ES2010/070534 ES2010070534W WO2011012764A3 WO 2011012764 A3 WO2011012764 A3 WO 2011012764A3 ES 2010070534 W ES2010070534 W ES 2010070534W WO 2011012764 A3 WO2011012764 A3 WO 2011012764A3
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Prior art keywords
stem cells
mesenchymal stem
identifying
analysing
cultures
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PCT/ES2010/070534
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English (en)
French (fr)
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WO2011012764A2 (es
Inventor
Enrique SAMPER RODRÍGUEZ
Juan Camilo ESTRADA RODRÍGUEZ
Antonio Bernad Miana
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Centro Nacional De Investigaciones Cardiovasculares (Cnic)
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Publication date
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Priority to US13/387,694 priority Critical patent/US20120165216A1/en
Priority to EP10803953.8A priority patent/EP2460876B1/en
Priority to ES10803953.8T priority patent/ES2445157T3/es
Publication of WO2011012764A2 publication Critical patent/WO2011012764A2/es
Publication of WO2011012764A3 publication Critical patent/WO2011012764A3/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0602—Vertebrate cells
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    • C12N5/0662—Stem cells
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
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    • C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
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    • C12Q2600/00—Oligonucleotides characterized by their use
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Abstract

La invención se refiere a un método para identificar células madre mesenquimales senescentes creciendo en cultivos in vitro, que comprende: medir la longitud de los telómeros, determinar el nivel de ploidía en la célula, analizar la presencia de mitosis multipolares y analizar la expresión de los genes SCIN, AKAP9, EDN-1, CXCL1, CXCL12 y CD70 y, además, de los genes HIST1H4C, HIST1H4L, HIST1H1C, CENPM, DYNLT3, SPC25, GTSEl, CDC45L, PLKl y SKA3. Este método puede ser de utilidad para la realización de estudios de estabilidad genética en los cultivos de células madre mesenquimales cuyas células van a ser empleadas en terapia celular, lo que permite identificar y seleccionar aquellos más estables y apropiados para tal fin.
PCT/ES2010/070534 2009-07-30 2010-07-30 Método de identificación de células madre mesenquimales senescentes WO2011012764A2 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US13/387,694 US20120165216A1 (en) 2009-07-30 2010-07-30 Method for identifying senescent mesenchymal stem cells
EP10803953.8A EP2460876B1 (en) 2009-07-30 2010-07-30 Method for identifying senescent mesenchymal stem cells
ES10803953.8T ES2445157T3 (es) 2009-07-30 2010-07-30 Método de identificación de células madre mesenquimales senescentes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP200930540 2009-07-30
ES200930540A ES2351916B8 (es) 2009-07-30 2009-07-30 Metodo de identificacion de celulas madre mesenquimales senescentes

Publications (2)

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WO2011012764A2 WO2011012764A2 (es) 2011-02-03
WO2011012764A3 true WO2011012764A3 (es) 2011-07-14

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PCT/ES2010/070534 WO2011012764A2 (es) 2009-07-30 2010-07-30 Método de identificación de células madre mesenquimales senescentes

Country Status (4)

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US (1) US20120165216A1 (es)
EP (1) EP2460876B1 (es)
ES (2) ES2351916B8 (es)
WO (1) WO2011012764A2 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2016138847A (ru) * 2014-04-07 2018-05-07 Хэлф Ресерч, Инк. Композиции и способы, относящиеся к покоящимся предрасположенным к старению клеткам (DSPC)
EP3091084A1 (en) * 2015-05-08 2016-11-09 Université Catholique De Louvain Methods for assessing the purity of a mesenchymal stem cells preparation
WO2016196765A2 (en) * 2015-06-03 2016-12-08 Aelan Cell Technologies, Inc. Companion methods and kits for il-2-based therapies and mesenchymal stem cell-based therapies
US11291689B2 (en) 2015-06-03 2022-04-05 Aelan Cell Technologies, Inc. Methods and devices for the production and delivery of beneficial factors from adipose-derived stem cells
CN114574570B (zh) * 2022-04-08 2024-06-04 青岛啤酒股份有限公司 一种啤酒酵母高代数使用的评估方法及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009073518A1 (en) * 2007-11-30 2009-06-11 New York Medical College Methods of isolating non-senescent cardiac stem cells and uses thereof

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
US6566399B2 (en) 2000-08-03 2003-05-20 Byung-Moo Min Inhibitor of replicative senescence of human keratinocytes containing retinoic acid as active ingredients

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009073518A1 (en) * 2007-11-30 2009-06-11 New York Medical College Methods of isolating non-senescent cardiac stem cells and uses thereof

Non-Patent Citations (5)

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Title
CHAMBERS, S.M. ET AL.: "Aging hematopoietic stem cells decline in function and exhibit epigenetic dysregulation.", PLOS BIOLOGY., vol. 5, no. 8, August 2007 (2007-08-01), pages E201., XP008151459 *
FUNAYAMA, R. ET AL.: "Loss of linker histone H1 in cellular senescence.", THE JOURNAL OF CELL BIOLOGY, vol. 175, no. 6, 18 December 2006 (2006-12-18), pages 869 - 880, XP008151462 *
LECHEL, A. ET AL.: "The cellular level of telomere dysfunction determines induction of senescence or apoptosis in vivo.", EMBO REPORTS., vol. 6, no. 3, March 2005 (2005-03-01), pages 275 - 281, XP008151458 *
MAEHARA, K. ET AL.: "CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses.", MOLECULAR AND CELLULAR BIOLOGY., vol. 30, no. 9, 16 February 2010 (2010-02-16), pages 2090 - 2104, XP008151461 *
WALL, LB. ET AL.: "Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers.", THE JOURNAL OF INVESTIGATIVE DERMATOLOGY., vol. 128, no. 10, October 2008 (2008-10-01), pages 2526 - 2540, XP008151460 *

Also Published As

Publication number Publication date
EP2460876B1 (en) 2013-09-04
ES2351916A1 (es) 2011-02-14
EP2460876A4 (en) 2012-12-26
ES2351916B1 (es) 2011-12-29
US20120165216A1 (en) 2012-06-28
EP2460876A2 (en) 2012-06-06
ES2351916B8 (es) 2012-07-09
WO2011012764A2 (es) 2011-02-03
ES2445157T3 (es) 2014-02-28

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