ES2421543T3 - Método para la preparación de virus y proteínas del PRRS, y kits de pruebas de diagnóstico para su detección - Google Patents

Método para la preparación de virus y proteínas del PRRS, y kits de pruebas de diagnóstico para su detección

Info

Publication number
ES2421543T3
ES2421543T3 ES06774559T ES06774559T ES2421543T3 ES 2421543 T3 ES2421543 T3 ES 2421543T3 ES 06774559 T ES06774559 T ES 06774559T ES 06774559 T ES06774559 T ES 06774559T ES 2421543 T3 ES2421543 T3 ES 2421543T3
Authority
ES
Spain
Prior art keywords
prrs virus
cells
proteins
preparation
antigens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES06774559T
Other languages
English (en)
Inventor
Eugenio P Mende
Han-Soo Joo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Minnesota
Original Assignee
University of Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Minnesota filed Critical University of Minnesota
Application granted granted Critical
Publication of ES2421543T3 publication Critical patent/ES2421543T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C12N7/025—Packaging cell lines, e.g. transcomplementing cell lines, for production of virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54391—Immunochromatographic test strips based on vertical flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552—Veterinary vaccine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/10011—Arteriviridae
    • C12N2770/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/10011—Arteriviridae
    • C12N2770/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)

Abstract

Un método para la preparación de una composición vacunal que contiene proteínas y antígenos del virus delPRRS a partir de células infectadas por el virus PRRS, comprendiendo dicho método: (a) proporcionar una población de células infectada con el virus del PRRS; (b) separar dichas células infectadas del virus del PRRS libre de células para formar células aisladas quecontengan proteínas y antígenos del virus del PRRS asociadas con las células; y (c) extraer o eluir proteínas y antígenos del virus del PRRS de las células aisladas con una solución quecontenga detergente para producir una preparación que comprenda proteínas y antígenos del virus del PRRS;y (d) combinar dicho extracto o eluido con un adyuvante para formar una composición vacunal.
ES06774559T 2005-07-05 2006-06-30 Método para la preparación de virus y proteínas del PRRS, y kits de pruebas de diagnóstico para su detección Active ES2421543T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/175,605 US7241582B2 (en) 2005-07-05 2005-07-05 Diagnostic test kits
PCT/US2006/026456 WO2007006031A2 (en) 2005-07-05 2006-06-30 Method for the preparation of prrs virus and proteins of and diagnostic test kits for detecting them

Publications (1)

Publication Number Publication Date
ES2421543T3 true ES2421543T3 (es) 2013-09-03

Family

ID=37492409

Family Applications (1)

Application Number Title Priority Date Filing Date
ES06774559T Active ES2421543T3 (es) 2005-07-05 2006-06-30 Método para la preparación de virus y proteínas del PRRS, y kits de pruebas de diagnóstico para su detección

Country Status (12)

Country Link
US (4) US7241582B2 (es)
EP (1) EP1904627B1 (es)
JP (2) JP2009501901A (es)
KR (2) KR101618066B1 (es)
CN (1) CN101253261B (es)
CA (1) CA2614332C (es)
DK (1) DK1904627T3 (es)
ES (1) ES2421543T3 (es)
MX (1) MX340547B (es)
PL (1) PL1904627T3 (es)
TW (1) TWI432447B (es)
WO (1) WO2007006031A2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7241582B2 (en) 2005-07-05 2007-07-10 Mj Biologics, Inc. Diagnostic test kits
KR101101386B1 (ko) * 2008-12-04 2012-01-02 주식회사 바이오리쏘스 북미형 ｐｒｒｓｖ에 특이적인 면역원성 펩티드 및 그의 용도
US8012770B2 (en) 2009-07-31 2011-09-06 Invisible Sentinel, Inc. Device for detection of antigens and uses thereof
US9475049B2 (en) 2009-07-31 2016-10-25 Invisible Sentinel, Inc. Analyte detection devices, multiplex and tabletop devices for detection of analyte, and uses thereof
US20120164232A1 (en) * 2009-09-09 2012-06-28 Tokyo Institute Of Technology Construct coated with virus coat-constituting protein and method for producing same
EP2486120B1 (en) 2009-10-09 2014-04-02 Invisible Sentinel, Inc. Device for detection of antigens and uses thereof
EP2488872B1 (en) * 2009-10-13 2014-04-16 Fraunhofer-Gesellschaft zur Förderung der Angewandten Forschung e.V. Methods for prrsv detection
EP3578980B1 (en) 2012-03-09 2021-09-15 Invisible Sentinel, Inc. Methods and compositions for detecting multiple analytes with a single signal
CN103364551A (zh) * 2012-04-01 2013-10-23 武汉中博生物股份有限公司 猪繁殖与呼吸综合征病毒IgM抗体ELISA检测试剂盒及其制备方法和其应用
KR101438089B1 (ko) * 2012-12-18 2014-11-03 대한민국(관리부서 : 농림축산식품부 농림축산검역본부) 돼지생식기호흡기증후군 바이러스 검출용 바이오 프로브 및 이를 이용한 돼지생식기호흡기증후군 바이러스 진단 방법
WO2016130569A1 (en) 2015-02-09 2016-08-18 Mj Biologics, Inc. A composition comprising pedv antigens and methods for making and using the composition
ES2682084T3 (es) 2014-02-07 2018-09-18 Mj Biologics, Inc. Proteínas y antígenos del virus de la diarrea epidémica porcina (VDEP)
US10280199B2 (en) * 2014-02-07 2019-05-07 Phibro Animal Health Corporation Coronavirus proteins and antigens
CN104792985A (zh) * 2015-04-21 2015-07-22 乾元浩生物股份有限公司 定性定量分析禽用禽流感病毒抗原有效成分的方法
US10279031B2 (en) 2016-05-11 2019-05-07 Phibro Animal Health Corporation Composition comprising antigens and a mucosal adjuvant and a method for using
CN110894243B (zh) * 2019-12-16 2021-07-13 中国农业大学 一种猪蓝耳病毒嵌合抗原以及检测猪蓝耳病毒抗体的胶体金免疫层析试纸条
CN116359498B (zh) * 2023-02-23 2024-06-11 兰州兽研生物科技有限公司 一种口蹄疫、非洲猪瘟、猪瘟、猪繁殖与呼吸综合征病毒抗体联检的免疫层析试纸卡

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4562147A (en) 1983-06-13 1985-12-31 Regents Of The University Of Minnesota Method and kit for diagnosis of pseudorabies
US6251397B1 (en) * 1992-10-30 2001-06-26 Iowa State University Research Foundation, Inc. Proteins encoded by polynucleic acids isolated from a porcine reproductive and respiratory syndrome virus and immunogenic compositions containing the same
US6380376B1 (en) * 1992-10-30 2002-04-30 Iowa State University Research Foundation Proteins encoded by polynucleic acids of porcine reproductive and respiratory syndrome virus (PRRSV)
US6592873B1 (en) * 1992-10-30 2003-07-15 Iowa State University Research Foundation, Inc. Polynucleic acids isolated from a porcine reproductive and respiratory syndrome virus (PRRSV) and proteins encoded by the polynucleic acids
US6773908B1 (en) * 1992-10-30 2004-08-10 Iowa State University Research Foundation, Inc. Proteins encoded by polynucleic acids of porcine reproductive and respiratory syndrome virus (PRRSV)
GB2289279B (en) * 1994-05-13 1998-09-16 Iberica Cyanamid Diagnostic kits and vaccines containing recombinant PRRSV proteins
US5690940A (en) 1995-06-21 1997-11-25 Regents Of The University Of Minnesota Low pathogencity PRRS live virus vaccines and methods of preparation thereof
AU3252999A (en) 1998-02-06 1999-08-23 Societe Des Produits Nestle S.A. Method for continuous preparation of a garnished pastry
US7241582B2 (en) 2005-07-05 2007-07-10 Mj Biologics, Inc. Diagnostic test kits

Also Published As

Publication number Publication date
CA2614332C (en) 2021-03-02
KR101541275B1 (ko) 2015-08-03
US20100278859A1 (en) 2010-11-04
PL1904627T3 (pl) 2013-12-31
EP1904627A2 (en) 2008-04-02
US20160287691A1 (en) 2016-10-06
US20070212684A1 (en) 2007-09-13
US7241582B2 (en) 2007-07-10
KR20080055791A (ko) 2008-06-19
TWI432447B (zh) 2014-04-01
TW200740840A (en) 2007-11-01
CA2614332A1 (en) 2007-01-11
WO2007006031A3 (en) 2007-04-05
CN101253261B (zh) 2012-11-28
KR20140033493A (ko) 2014-03-18
CN101253261A (zh) 2008-08-27
KR101618066B1 (ko) 2016-05-04
DK1904627T3 (da) 2013-08-05
US7776537B2 (en) 2010-08-17
WO2007006031A2 (en) 2007-01-11
US9849171B2 (en) 2017-12-26
JP2013135688A (ja) 2013-07-11
EP1904627B1 (en) 2013-05-22
US20070009911A1 (en) 2007-01-11
US9388218B2 (en) 2016-07-12
JP2009501901A (ja) 2009-01-22
MX340547B (es) 2016-07-13

Similar Documents

Publication Publication Date Title
ES2421543T3 (es) Método para la preparación de virus y proteínas del PRRS, y kits de pruebas de diagnóstico para su detección
Mikryukova et al. Surveillance of tick-borne encephalitis virus in wild birds and ticks in Tomsk city and its suburbs (Western Siberia)
EA200900784A1 (ru) Вакцины, включающие антиген из четырех штаммов вируса гриппа
CL2013003248A1 (es) Composición inmunogenica que comprende una glucoproteina g de los virus hendra y nipah y un complejo inmunoestimulante; método para producir una respuesta de anticuerpo neutralizante contra un virus hendra y/o nipah en un sujeto.
AR071220A1 (es) Astrovirus aviario depositado bajo el nuero cncm i-3895
AR059772A1 (es) Composiciones que incuyen hemaglutinina metodos de elaboracion y metodos de uso de las mismas
AR068507A1 (es) Construcciones vector fluorocarbonado-antigeno para el suministro de antigenos ifluenza
PE20071098A1 (es) Composicion inmugenica que contiene como adyuvantes una saponina y un polisacarido
AR061894A1 (es) Vacunas para malaria
ES2627330T3 (es) Uso de JAZ5a para mejorar la resistencia a la sequía en una planta
BR112014015898A2 (pt) Composições de vacina para a síndrome reprodutiva e respiratória do suíno (prrs)compreendendo antígeno de peptídeo, uso de antígeno de peptídeo para proteção de porquinhos contra infecção por prrs, bem como imunoensaio elisa para teste da presença de anticorpos anti-prrsv
PE20081686A1 (es) Proteinas de fusion que comprenden un antigeno prame (dage)
Cao et al. Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo
CR6491A (es) Composiciones y metodos inmunogenicos de hiv
AR066449A1 (es) Metodo para la purificacion de un virus o antigeno viral, por ultracentrifugacion continua, usando formulaciones de soluciones de sacarosa
EA201071086A1 (ru) Усовершенствованный способ получения вакцинных антигенов вируса гриппа
Ding et al. Proteomic alteration of Marc-145 cells and PAMs after infection by porcine reproductive and respiratory syndrome virus
AR034216A1 (es) Metodo de extraccion de virus a partir de cultivos celulares y composiciones que comprenden dicho virus
GEP201706766B (en) Methods and compositions for vaccinating against staphylococcus aureus
Ouyang et al. Comparative analysis of routes of immunization of a live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in a heterologous virus challenge study
NO20081909L (no) Bovint viralt diare virus (BVDV), samt BVDV-vaksine
AR087654A1 (es) METODO DIAGNOSTICO DE ANIMALES VACUNADOS CON PESTIVIRUS QUIMERICO EN EL CUAL LA PROTEINA Eʳⁿˢ DEL VIRUS DE LA DIARREA VIRAL BOVINA (BVDV) ES REMPLAZADA POR PROTEINA Eʳⁿˢ DE PESTIVIRUS DE ANTILOPE AMERICANO
GB2429711A (en) Heart and skeletal muscle inflammation (HSMI) virus
AR051853A1 (es) Vacuna para aumentar el crecimiento basada en epitopes neutralizantes
Kaabinejadian et al. Identification of class I HLA T cell control epitopes for West Nile virus