ES2376866T3 - Células troncales hemotopoyéticas y procedimientos de tratamiento de las enfermedades oculares noeovasculares con las mismas - Google Patents
Células troncales hemotopoyéticas y procedimientos de tratamiento de las enfermedades oculares noeovasculares con las mismas Download PDFInfo
- Publication number
- ES2376866T3 ES2376866T3 ES03772195T ES03772195T ES2376866T3 ES 2376866 T3 ES2376866 T3 ES 2376866T3 ES 03772195 T ES03772195 T ES 03772195T ES 03772195 T ES03772195 T ES 03772195T ES 2376866 T3 ES2376866 T3 ES 2376866T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- population
- retinal
- lin
- negative
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 42
- 238000011282 treatment Methods 0.000 title claims abstract description 24
- 208000030533 eye disease Diseases 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title description 13
- 210000001185 bone marrow Anatomy 0.000 claims abstract description 42
- 230000003511 endothelial effect Effects 0.000 claims abstract description 21
- 238000002360 preparation method Methods 0.000 claims abstract description 19
- 239000003814 drug Substances 0.000 claims abstract description 18
- 230000003394 haemopoietic effect Effects 0.000 claims abstract description 18
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 15
- 201000010099 disease Diseases 0.000 claims abstract description 14
- 229940079593 drug Drugs 0.000 claims abstract description 9
- 210000004027 cell Anatomy 0.000 claims description 149
- 230000002792 vascular Effects 0.000 claims description 55
- 230000002207 retinal effect Effects 0.000 claims description 52
- 108090000623 proteins and genes Proteins 0.000 claims description 36
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 claims description 27
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 22
- 210000001616 monocyte Anatomy 0.000 claims description 22
- 102000016971 Proto-Oncogene Proteins c-kit Human genes 0.000 claims description 18
- 108010014608 Proto-Oncogene Proteins c-kit Proteins 0.000 claims description 18
- 208000017442 Retinal disease Diseases 0.000 claims description 14
- 239000012634 fragment Substances 0.000 claims description 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 13
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 12
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 claims description 12
- 241000124008 Mammalia Species 0.000 claims description 12
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 claims description 12
- 230000005764 inhibitory process Effects 0.000 claims description 10
- 206010012689 Diabetic retinopathy Diseases 0.000 claims description 9
- 230000001772 anti-angiogenic effect Effects 0.000 claims description 9
- 230000000508 neurotrophic effect Effects 0.000 claims description 9
- 230000004263 retinal angiogenesis Effects 0.000 claims description 9
- 230000003412 degenerative effect Effects 0.000 claims description 8
- 206010064930 age-related macular degeneration Diseases 0.000 claims description 6
- 229960002685 biotin Drugs 0.000 claims description 6
- 235000020958 biotin Nutrition 0.000 claims description 6
- 239000011616 biotin Substances 0.000 claims description 6
- 208000002780 macular degeneration Diseases 0.000 claims description 6
- 206010038923 Retinopathy Diseases 0.000 claims description 5
- 239000002771 cell marker Substances 0.000 claims description 5
- 230000000302 ischemic effect Effects 0.000 claims description 5
- 238000002372 labelling Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- 206010057430 Retinal injury Diseases 0.000 claims description 4
- 208000007014 Retinitis pigmentosa Diseases 0.000 claims description 3
- 208000007135 Retinal Neovascularization Diseases 0.000 claims description 2
- 206010038933 Retinopathy of prematurity Diseases 0.000 claims description 2
- 230000002491 angiogenic effect Effects 0.000 claims description 2
- 230000000740 bleeding effect Effects 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 claims description 2
- 230000000087 stabilizing effect Effects 0.000 claims description 2
- 108050005493 CD3 protein, epsilon/gamma/delta subunit Proteins 0.000 claims 8
- 101001065556 Mus musculus Lymphocyte antigen 6G Proteins 0.000 claims 8
- 238000000605 extraction Methods 0.000 claims 3
- 101000640990 Arabidopsis thaliana Tryptophan-tRNA ligase, chloroplastic/mitochondrial Proteins 0.000 claims 2
- 102100034300 Tryptophan-tRNA ligase, cytoplasmic Human genes 0.000 claims 2
- 201000002563 Histoplasmosis Diseases 0.000 claims 1
- 208000007502 anemia Diseases 0.000 claims 1
- 230000035800 maturation Effects 0.000 claims 1
- 210000001508 eye Anatomy 0.000 description 77
- 210000001525 retina Anatomy 0.000 description 66
- 241000699670 Mus sp. Species 0.000 description 42
- 239000010410 layer Substances 0.000 description 39
- 239000007924 injection Substances 0.000 description 36
- 238000002347 injection Methods 0.000 description 36
- 241000699666 Mus <mouse, genus> Species 0.000 description 35
- 230000004233 retinal vasculature Effects 0.000 description 26
- 210000005166 vasculature Anatomy 0.000 description 25
- 210000001130 astrocyte Anatomy 0.000 description 22
- 210000003995 blood forming stem cell Anatomy 0.000 description 20
- 108010053099 Vascular Endothelial Growth Factor Receptor-2 Proteins 0.000 description 15
- 239000003550 marker Substances 0.000 description 15
- 230000000694 effects Effects 0.000 description 12
- 239000005090 green fluorescent protein Substances 0.000 description 12
- 210000001956 EPC Anatomy 0.000 description 11
- 230000015572 biosynthetic process Effects 0.000 description 11
- 210000002889 endothelial cell Anatomy 0.000 description 11
- 210000001210 retinal vessel Anatomy 0.000 description 11
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 10
- 230000001537 neural effect Effects 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 102100039289 Glial fibrillary acidic protein Human genes 0.000 description 9
- 101710193519 Glial fibrillary acidic protein Proteins 0.000 description 9
- 210000004204 blood vessel Anatomy 0.000 description 9
- 210000005046 glial fibrillary acidic protein Anatomy 0.000 description 9
- 101100481408 Danio rerio tie2 gene Proteins 0.000 description 8
- 101100481410 Mus musculus Tek gene Proteins 0.000 description 8
- 102000016549 Vascular Endothelial Growth Factor Receptor-2 Human genes 0.000 description 8
- 230000033115 angiogenesis Effects 0.000 description 8
- 230000007850 degeneration Effects 0.000 description 8
- 239000011159 matrix material Substances 0.000 description 8
- 238000010186 staining Methods 0.000 description 8
- 201000007737 Retinal degeneration Diseases 0.000 description 7
- 230000002159 abnormal effect Effects 0.000 description 7
- 238000011161 development Methods 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 7
- 230000003902 lesion Effects 0.000 description 7
- 230000004258 retinal degeneration Effects 0.000 description 7
- 230000006459 vascular development Effects 0.000 description 7
- 230000003140 astrocytic effect Effects 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 206010018341 Gliosis Diseases 0.000 description 5
- 210000002798 bone marrow cell Anatomy 0.000 description 5
- 238000012512 characterization method Methods 0.000 description 5
- 230000007387 gliosis Effects 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 210000004498 neuroglial cell Anatomy 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 238000011771 FVB mouse Methods 0.000 description 4
- 241001529936 Murinae Species 0.000 description 4
- 206010029113 Neovascularisation Diseases 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 4
- 238000010348 incorporation Methods 0.000 description 4
- 208000015122 neurodegenerative disease Diseases 0.000 description 4
- 210000001328 optic nerve Anatomy 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 230000009261 transgenic effect Effects 0.000 description 4
- 238000011830 transgenic mouse model Methods 0.000 description 4
- 230000004382 visual function Effects 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 208000003098 Ganglion Cysts Diseases 0.000 description 3
- 208000032843 Hemorrhage Diseases 0.000 description 3
- 206010021143 Hypoxia Diseases 0.000 description 3
- 208000034038 Pathologic Neovascularization Diseases 0.000 description 3
- 208000005400 Synovial Cyst Diseases 0.000 description 3
- 210000001056 activated astrocyte Anatomy 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000003102 growth factor Substances 0.000 description 3
- 208000028867 ischemia Diseases 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 210000003583 retinal pigment epithelium Anatomy 0.000 description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 238000011735 C3H mouse Methods 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 2
- 101500024236 Homo sapiens T2-TrpRS Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 241000699660 Mus musculus Species 0.000 description 2
- 206010030113 Oedema Diseases 0.000 description 2
- 206010063664 Presumed ocular histoplasmosis syndrome Diseases 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 102100033177 Vascular endothelial growth factor receptor 2 Human genes 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000964 angiostatic effect Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 238000001415 gene therapy Methods 0.000 description 2
- 230000002518 glial effect Effects 0.000 description 2
- 230000007954 hypoxia Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 210000002569 neuron Anatomy 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 230000000649 photocoagulation Effects 0.000 description 2
- 108091008695 photoreceptors Proteins 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 230000004393 visual impairment Effects 0.000 description 2
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 description 1
- HVAKUYCEWDPRCA-IZZDOVSWSA-N (e)-1-(2,4-dimethoxyphenyl)-3-(4-methoxyphenyl)prop-2-en-1-one Chemical compound C1=CC(OC)=CC=C1\C=C\C(=O)C1=CC=C(OC)C=C1OC HVAKUYCEWDPRCA-IZZDOVSWSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 102000008076 Angiogenic Proteins Human genes 0.000 description 1
- 108010074415 Angiogenic Proteins Proteins 0.000 description 1
- 102000004550 Angiostatic Proteins Human genes 0.000 description 1
- 108010017551 Angiostatic Proteins Proteins 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 201000004569 Blindness Diseases 0.000 description 1
- 102100034871 C-C motif chemokine 8 Human genes 0.000 description 1
- 101710155833 C-C motif chemokine 8 Proteins 0.000 description 1
- 101150000551 CRYAA gene Proteins 0.000 description 1
- 101150074495 CRYBA1 gene Proteins 0.000 description 1
- 101150013498 CRYBB2 gene Proteins 0.000 description 1
- 101150053424 CRYGC gene Proteins 0.000 description 1
- 101150092921 CXADR gene Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241001092081 Carpenteria Species 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 101150114072 Crygd gene Proteins 0.000 description 1
- 101150104864 Crygf gene Proteins 0.000 description 1
- 101150080484 Crygs gene Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 238000000116 DAPI staining Methods 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 101150047444 H2-Aa gene Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 101150051253 KCNJ5 gene Proteins 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 101710157814 Lysozyme P Proteins 0.000 description 1
- 102000012750 Membrane Glycoproteins Human genes 0.000 description 1
- 108010090054 Membrane Glycoproteins Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 101100182712 Mus musculus Ly6a gene Proteins 0.000 description 1
- 101100344220 Mus musculus Lyz1 gene Proteins 0.000 description 1
- 101100369238 Mus musculus Tgtp2 gene Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 208000014245 Ocular vascular disease Diseases 0.000 description 1
- 239000012124 Opti-MEM Substances 0.000 description 1
- 101710160107 Outer membrane protein A Proteins 0.000 description 1
- 101150014518 PIP5K1C gene Proteins 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 208000031481 Pathologic Constriction Diseases 0.000 description 1
- 102000004257 Potassium Channel Human genes 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 208000014139 Retinal vascular disease Diseases 0.000 description 1
- 206010038910 Retinitis Diseases 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- -1 Sca-1 Proteins 0.000 description 1
- 102000008063 Small Heat-Shock Proteins Human genes 0.000 description 1
- 101710120037 Toxin CcdB Proteins 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 208000024248 Vascular System injury Diseases 0.000 description 1
- 208000012339 Vascular injury Diseases 0.000 description 1
- 108091005971 Wild-type GFP Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 102000004586 YY1 Transcription Factor Human genes 0.000 description 1
- 108010042669 YY1 Transcription Factor Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000000961 alloantigen Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000003587 angiostatic protein Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000003367 anti-collagen effect Effects 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 229960000846 camphor Drugs 0.000 description 1
- 210000004413 cardiac myocyte Anatomy 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 210000003986 cell retinal photoreceptor Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000008045 co-localization Effects 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000002074 deregulated effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- FHIVAFMUCKRCQO-UHFFFAOYSA-N diazinon Chemical compound CCOP(=S)(OCC)OC1=CC(C)=NC(C(C)C)=N1 FHIVAFMUCKRCQO-UHFFFAOYSA-N 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 210000000744 eyelid Anatomy 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000002825 functional assay Methods 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 238000011331 genomic analysis Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 210000003566 hemangioblast Anatomy 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 230000001146 hypoxic effect Effects 0.000 description 1
- 238000002991 immunohistochemical analysis Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 239000007925 intracardiac injection Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 238000002826 magnetic-activated cell sorting Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- AEUKDPKXTPNBNY-XEYRWQBLSA-N mcp 2 Chemical compound C([C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CS)NC(=O)[C@H](C)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)C1=CC=CC=C1 AEUKDPKXTPNBNY-XEYRWQBLSA-N 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 210000000274 microglia Anatomy 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 230000014399 negative regulation of angiogenesis Effects 0.000 description 1
- 210000001178 neural stem cell Anatomy 0.000 description 1
- 230000003955 neuronal function Effects 0.000 description 1
- 238000012758 nuclear staining Methods 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 210000003733 optic disk Anatomy 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 210000003668 pericyte Anatomy 0.000 description 1
- 239000002831 pharmacologic agent Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 108020001213 potassium channel Proteins 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 101150053856 psmb9 gene Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000020874 response to hypoxia Effects 0.000 description 1
- 230000004242 retinal defects Effects 0.000 description 1
- 210000001116 retinal neuron Anatomy 0.000 description 1
- 230000004276 retinal vascularization Effects 0.000 description 1
- 210000003786 sclera Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 208000007056 sickle cell anemia Diseases 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 108091052270 small heat shock protein (HSP20) family Proteins 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 230000036262 stenosis Effects 0.000 description 1
- 208000037804 stenosis Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 239000012096 transfection reagent Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 230000006496 vascular abnormality Effects 0.000 description 1
- 230000007998 vessel formation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/069—Vascular Endothelial cells
- C12N5/0692—Stem cells; Progenitor cells; Precursor cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/14—Vasoprotectives; Antihaemorrhoidals; Drugs for varicose therapy; Capillary stabilisers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Vascular Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Epidemiology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Diabetes (AREA)
- Virology (AREA)
- Transplantation (AREA)
- Ophthalmology & Optometry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39852202P | 2002-07-25 | 2002-07-25 | |
| US398522P | 2002-07-25 | ||
| US46705103P | 2003-05-02 | 2003-05-02 | |
| US467051P | 2003-05-02 | ||
| PCT/US2003/024839 WO2004010959A2 (en) | 2002-07-25 | 2003-07-25 | Hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2376866T3 true ES2376866T3 (es) | 2012-03-20 |
Family
ID=31191212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES03772195T Expired - Lifetime ES2376866T3 (es) | 2002-07-25 | 2003-07-25 | Células troncales hemotopoyéticas y procedimientos de tratamiento de las enfermedades oculares noeovasculares con las mismas |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US7153501B2 (https=) |
| EP (1) | EP1542536B1 (https=) |
| JP (2) | JP2005538742A (https=) |
| KR (2) | KR101095287B1 (https=) |
| CN (1) | CN1307300C (https=) |
| AT (1) | ATE536405T1 (https=) |
| AU (1) | AU2003259687B2 (https=) |
| BR (1) | BR0312920A (https=) |
| CA (1) | CA2493122C (https=) |
| DK (1) | DK1542536T3 (https=) |
| ES (1) | ES2376866T3 (https=) |
| MX (1) | MXPA05000972A (https=) |
| PL (1) | PL216193B1 (https=) |
| PT (1) | PT1542536E (https=) |
| RU (1) | RU2331669C2 (https=) |
| WO (2) | WO2004010959A2 (https=) |
| ZA (1) | ZA200500682B (https=) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060104962A1 (en) * | 2002-07-25 | 2006-05-18 | The Scripps Research Institute | Transfected hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith |
| WO2006031467A2 (en) * | 2002-07-25 | 2006-03-23 | The Scripps Research Institute | Isolated lineage negative hematopoietic stem cells and methods of treatment therewith |
| US20060104963A1 (en) * | 2002-07-25 | 2006-05-18 | The Scripps Research Institute | Treatment of cone cell degeneration with transfected lineage negative hematopoietic stem cells |
| BRPI0409861A (pt) * | 2003-05-02 | 2006-05-16 | Scripps Research Inst | células- tronco hematopoiéticas e sua utilização em métodos de tratamento de doenças oculares neovasculares |
| WO2005042731A1 (en) * | 2003-10-31 | 2005-05-12 | Children's Medical Center Corporation | Methods for purifying hematopoietic stem cells |
| RU2279886C2 (ru) * | 2004-06-10 | 2006-07-20 | Московский научно-исследовательский институт глазных болезней им. Гельмгольца Минздрава РФ | Способ улучшения функциональной активности сетчатки при ее патологии различного генеза |
| US8282921B2 (en) | 2004-08-02 | 2012-10-09 | Paul Glidden | tRNA synthetase fragments |
| ATE423842T1 (de) * | 2004-08-13 | 2009-03-15 | Medtronic Inc | Isolation der endothelvorläuferzellen-untermengen und verfahren zu ihrer verwendung |
| JP2008512376A (ja) * | 2004-09-03 | 2008-04-24 | ザ・スクリプス・リサーチ・インステイチユート | 単離された系統陰性造血幹細胞及びこれを用いた治療方法 |
| DE102004055615A1 (de) * | 2004-11-16 | 2006-05-18 | Universitätsklinikum Hamburg-Eppendorf | In vitro aus Knochenmarkstammzellen differenzierte Retina-spezifische Zellen, ihre Herstellung und Verwendung |
| CA2598029A1 (en) * | 2005-02-24 | 2006-10-05 | The Scripps Research Institute | Isolated myeloid-like bone marrow cell populations and methods of treatment therewith |
| AU2006216473B2 (en) * | 2005-02-24 | 2012-07-26 | The Scripps Research Institute | Method for the treatment of retinopathy of prematurity and related retinopathic diseases |
| US20070231306A1 (en) * | 2005-02-24 | 2007-10-04 | The Scripps Research Institute | Isolated myeloid-like cell populations and methods of treatment therewith |
| AU2006216678B2 (en) * | 2005-02-24 | 2011-11-17 | The Scripps Research Institute | Revascularization of ischemic retinal tissue and screening method therefor |
| JP4734636B2 (ja) * | 2005-11-02 | 2011-07-27 | 国立大学法人 鹿児島大学 | 免疫染色法、及び当該免疫染色法を利用した細胞の評価方法 |
| US20110076255A1 (en) | 2005-11-07 | 2011-03-31 | Pecora Andrew L | Compositions and methods for treating progressive myocardial injury due to a vascular insufficiency |
| MY147516A (en) | 2005-11-07 | 2012-12-31 | Amorcyte Inc | Compositions and method of vascular injury repair cross-reference to related applications |
| US8637005B2 (en) | 2005-11-07 | 2014-01-28 | Amorcyte, Inc. | Compositions and methods of vascular injury repair |
| EP2422802A3 (en) | 2006-03-07 | 2013-03-06 | Geeta Shroff | Compositions comprising human embryonic stem cells and their derivatives, methods of use, and methods of preparation |
| KR100757265B1 (ko) * | 2006-04-27 | 2007-09-10 | (주) 차바이오텍 | 제대혈 유래 줄기 세포를 포함하는 요실금 치료용 조성물 |
| JP6041456B2 (ja) * | 2006-07-12 | 2016-12-07 | メゾブラスト，インコーポレーテッド | 過剰新生血管形成の治療 |
| WO2008057580A2 (en) * | 2006-11-07 | 2008-05-15 | Pervasis Therapeutics, Inc. | Materials and methods for treating and managing angiogenesis-mediated diseases |
| US20100098675A1 (en) * | 2008-05-30 | 2010-04-22 | Nikolai Tankovich | Methods for reducing the side effects of ophthalmic laser surgery |
| BRPI0919020B8 (pt) * | 2008-09-12 | 2021-05-25 | Cryopraxis Criobiologia Ltda | uso de células de linhagem monocitária enriquecidas para tratar isquemia e para tratar angina pectoris |
| CN102245189B (zh) | 2008-12-03 | 2015-06-17 | 阿莫塞特公司 | 改善梗死区灌注的组合物以及血管损伤修复方法 |
| RU2428956C2 (ru) * | 2009-07-13 | 2011-09-20 | Александр Дмитриевич Ромащенко | Способ лечения атрофии зрительного нерва посредством трансплантации аутологичных стволовых клеток |
| WO2012005763A1 (en) | 2010-07-06 | 2012-01-12 | The Scripps Research Institute | Use of myeloid-like progenitor cell populations to treat tumors |
| KR102000141B1 (ko) | 2011-10-27 | 2019-07-15 | 웰스테이트 옵탈믹스 코퍼레이션 | 간상세포 유래 원추세포 생존능 인자를 인코딩하는 벡터 |
| AU2013249786A1 (en) * | 2012-04-16 | 2014-11-06 | The Regents Of The University Of California | Ocular therapeutics using embryonic stem cell microvesicles |
| BR112015003839B1 (pt) | 2012-08-24 | 2021-12-28 | Riken | Método para produzir lâmina de células do epitélio pigmentar da retina |
| WO2020172854A1 (en) * | 2019-02-28 | 2020-09-03 | Shenzhen University | Use of endothelial progenitor cells (epcs) in rejuvenating microvasculature, preventing aging and treating age-related diseases |
| CN121550400A (zh) * | 2026-01-26 | 2026-02-24 | 天津医科大学眼科医院 | Crybb2重组蛋白在治疗和/或预防糖尿病性视网膜病变中的应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA27055C2 (uk) * | 1993-12-14 | 2000-02-28 | Центр Ембріональних Тканин "Емселл" | Лікарський препарат імуhозаміhhої дії hа осhові клітиhhої суспеhзії та спосіб лікуваhhя сиhдрому hабутого імуhодефіциту (віл-іhфекції) з використаhhям цього препарату |
| US6908763B1 (en) * | 1997-08-22 | 2005-06-21 | The Board Of Trustees Of The Leland Stanford Junior University | Mammalian common lymphoid progenitor cell |
| EP1073716B1 (en) * | 1998-04-28 | 2004-04-28 | Amersham Health AS | Improvements in or relating to separation processes |
| US6767737B1 (en) * | 1998-08-31 | 2004-07-27 | New York University | Stem cells bearing an FGF receptor on the cell surface |
| US6780627B1 (en) * | 2000-01-31 | 2004-08-24 | Millennium Pharmaceuticals, Inc. | 22438, 23553, 25278, and 26212 novel human sulfatases |
| AU2001284695A1 (en) * | 2000-07-31 | 2002-02-13 | New York Medical College | Methods and compositions for the repair and/or regeneration of damaged myocardium |
| ES2318063T3 (es) * | 2001-02-23 | 2009-05-01 | The Scripps Research Institute | Polipeptidos derivados de la triptofanil arnt sintetasa utiles para la regulacion de la angiogenesis. |
| JP2005528362A (ja) * | 2002-03-21 | 2005-09-22 | ユニヴァーシティ オヴ フロリダ | 血管新生の調節法 |
| US20060034767A1 (en) * | 2002-07-05 | 2006-02-16 | Roger Williams Medical Center | Targeting and tracking of cells to specific organs and tissues in vivo |
-
2003
- 2003-07-25 PL PL374868A patent/PL216193B1/pl unknown
- 2003-07-25 AU AU2003259687A patent/AU2003259687B2/en not_active Ceased
- 2003-07-25 AT AT03772195T patent/ATE536405T1/de active
- 2003-07-25 WO PCT/US2003/024839 patent/WO2004010959A2/en not_active Ceased
- 2003-07-25 JP JP2005505648A patent/JP2005538742A/ja active Pending
- 2003-07-25 BR BRPI0312920-9A patent/BR0312920A/pt not_active IP Right Cessation
- 2003-07-25 CN CNB038177358A patent/CN1307300C/zh not_active Expired - Fee Related
- 2003-07-25 PT PT03772195T patent/PT1542536E/pt unknown
- 2003-07-25 KR KR1020057001388A patent/KR101095287B1/ko not_active Expired - Fee Related
- 2003-07-25 US US10/628,783 patent/US7153501B2/en not_active Expired - Fee Related
- 2003-07-25 DK DK03772195.8T patent/DK1542536T3/da active
- 2003-07-25 MX MXPA05000972A patent/MXPA05000972A/es active IP Right Grant
- 2003-07-25 EP EP03772195A patent/EP1542536B1/en not_active Expired - Lifetime
- 2003-07-25 RU RU2005105065/13A patent/RU2331669C2/ru not_active IP Right Cessation
- 2003-07-25 ES ES03772195T patent/ES2376866T3/es not_active Expired - Lifetime
- 2003-07-25 KR KR1020117020059A patent/KR20110102961A/ko not_active Ceased
- 2003-07-25 CA CA2493122A patent/CA2493122C/en not_active Expired - Fee Related
-
2004
- 2004-04-28 WO PCT/US2004/012989 patent/WO2004098499A2/en not_active Ceased
-
2005
- 2005-01-24 ZA ZA200500682A patent/ZA200500682B/xx unknown
-
2006
- 2006-12-20 US US11/643,571 patent/US20070116685A1/en not_active Abandoned
-
2010
- 2010-06-23 JP JP2010142292A patent/JP5385219B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP5385219B2 (ja) | 2014-01-08 |
| EP1542536B1 (en) | 2011-12-07 |
| PT1542536E (pt) | 2012-02-14 |
| KR101095287B1 (ko) | 2011-12-16 |
| KR20050027132A (ko) | 2005-03-17 |
| WO2004098499A3 (en) | 2005-10-06 |
| EP1542536A2 (en) | 2005-06-22 |
| RU2331669C2 (ru) | 2008-08-20 |
| WO2004010959A3 (en) | 2005-04-07 |
| JP2005538742A (ja) | 2005-12-22 |
| ATE536405T1 (de) | 2011-12-15 |
| KR20110102961A (ko) | 2011-09-19 |
| BR0312920A (pt) | 2007-07-10 |
| DK1542536T3 (da) | 2012-01-23 |
| PL374868A1 (en) | 2005-11-14 |
| PL216193B1 (pl) | 2014-03-31 |
| AU2003259687B2 (en) | 2009-10-29 |
| RU2005105065A (ru) | 2005-09-10 |
| CA2493122C (en) | 2012-11-27 |
| CN1307300C (zh) | 2007-03-28 |
| CN1688196A (zh) | 2005-10-26 |
| CA2493122A1 (en) | 2004-02-05 |
| US20040197902A1 (en) | 2004-10-07 |
| WO2004010959A2 (en) | 2004-02-05 |
| AU2003259687A1 (en) | 2004-02-16 |
| EP1542536A4 (en) | 2007-05-16 |
| US7153501B2 (en) | 2006-12-26 |
| ZA200500682B (en) | 2006-07-26 |
| US20070116685A1 (en) | 2007-05-24 |
| JP2010285434A (ja) | 2010-12-24 |
| WO2004098499A2 (en) | 2004-11-18 |
| MXPA05000972A (es) | 2005-09-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2376866T3 (es) | Células troncales hemotopoyéticas y procedimientos de tratamiento de las enfermedades oculares noeovasculares con las mismas | |
| US20050063961A1 (en) | Hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith | |
| RU2473686C2 (ru) | Выделенные популяции миелоподобных клеток и способы лечения с использованием таких популяций | |
| ES2382357T3 (es) | Células de tipo mieloide transfectadas para el tratamiento de retinopatía de la prematuridad y enfermedades relacionadas | |
| RU2418856C2 (ru) | Изолированные популяции миелоидоподобных клеток костного мозга и способы лечения с ними | |
| US20060104963A1 (en) | Treatment of cone cell degeneration with transfected lineage negative hematopoietic stem cells | |
| JP4714580B2 (ja) | 造血幹細胞及び該細胞による眼の血管新生性疾患の治療方法 | |
| US20050129665A1 (en) | Isolated lineage negative hematopoietic stem cells and methods of treatment therewith | |
| CN101052305B (zh) | 分离的谱系阴性造血干细胞及用其治疗的方法 | |
| US20060104962A1 (en) | Transfected hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith |