ES2371088T3 - Transferencia de energía no fluorescente. - Google Patents
Transferencia de energía no fluorescente. Download PDFInfo
- Publication number
- ES2371088T3 ES2371088T3 ES06806056T ES06806056T ES2371088T3 ES 2371088 T3 ES2371088 T3 ES 2371088T3 ES 06806056 T ES06806056 T ES 06806056T ES 06806056 T ES06806056 T ES 06806056T ES 2371088 T3 ES2371088 T3 ES 2371088T3
- Authority
- ES
- Spain
- Prior art keywords
- fraction
- acceptor
- nucleic acid
- probe
- fluorescent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 0 CCC(C12)[C@@](C)CCC1C(CC1O)(C3C1OCC3)C(*CC(C)C1*C)C1C2=C Chemical compound CCC(C12)[C@@](C)CCC1C(CC1O)(C3C1OCC3)C(*CC(C)C1*C)C1C2=C 0.000 description 3
- NLZOMSQIIVFMDK-UHFFFAOYSA-N O=C(CC1)C2=C1CCO2 Chemical compound O=C(CC1)C2=C1CCO2 NLZOMSQIIVFMDK-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Luminescent Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US72420205P | 2005-10-05 | 2005-10-05 | |
| US724202P | 2005-10-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2371088T3 true ES2371088T3 (es) | 2011-12-27 |
Family
ID=37574999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES06806056T Active ES2371088T3 (es) | 2005-10-05 | 2006-10-05 | Transferencia de energía no fluorescente. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7741467B2 (https=) |
| EP (1) | EP1934374B1 (https=) |
| JP (1) | JP4843044B2 (https=) |
| CN (1) | CN101321878B (https=) |
| AT (1) | ATE519863T1 (https=) |
| CA (1) | CA2624917C (https=) |
| ES (1) | ES2371088T3 (https=) |
| WO (1) | WO2007039301A2 (https=) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| FI20070163A0 (fi) * | 2007-02-27 | 2007-02-27 | Hidex Oy | Parannettu homogeeninen luminesenssibiotesti |
| JP2010525333A (ja) * | 2007-04-18 | 2010-07-22 | ベクトン・ディキンソン・アンド・カンパニー | Ｓｅｒｓナノタグを用いる分析法 |
| US8206929B2 (en) * | 2009-01-07 | 2012-06-26 | Roche Molecular Systems, Inc. | Nucleic acid amplification with allele-specific suppression of sequence variants |
| EP2398868B1 (en) * | 2009-02-20 | 2016-06-15 | Perkinelmer Biosignal, Inc. | Multiplex assay methods and compositions |
| WO2010101947A2 (en) * | 2009-03-02 | 2010-09-10 | Cooperative Diagnostics, Llc | Rapid oligo probes |
| US8674080B2 (en) | 2009-04-09 | 2014-03-18 | Roche Molecular Systems, Inc. | Dye composition for liquid transfer control |
| EP3209798B1 (en) * | 2014-10-23 | 2022-07-27 | Ricardo Mancebo | Reagents and methods for isothermal chain reaction |
| US9745618B2 (en) | 2014-11-19 | 2017-08-29 | Roche Molecular Systems, Inc. | Photoblocked probes and methods for sequential detection of nucleic acids |
| US9920381B2 (en) | 2014-12-02 | 2018-03-20 | Roche Molecular Systems, Inc. | Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus |
| WO2017153566A1 (en) | 2016-03-11 | 2017-09-14 | Roche Diagnostics Gmbh | Compositions and methods for detection of zika virus |
| CN109154023B (zh) | 2016-05-27 | 2022-09-20 | 豪夫迈·罗氏有限公司 | 用于检测阴道毛滴虫的组合物和方法 |
| US10612101B2 (en) | 2016-05-27 | 2020-04-07 | Roche Molecular Systems, Inc. | Compositions and methods for detection of Mycoplasma genitalium |
| EP4645615A3 (en) * | 2016-06-03 | 2026-01-28 | The General Hospital Corporation | System and method for micro laser particles |
| IL291323B2 (en) | 2016-07-15 | 2023-09-01 | Am Chemicals Llc | Solid non-nucleoside supports and phosphoramidite building blocks for oligonucleotide synthesis |
| JP7483373B2 (ja) | 2016-07-29 | 2024-05-15 | ジュノー セラピューティクス インコーポレイテッド | 複製可能ウイルスの存在または非存在を評価するための方法 |
| ES3008759T3 (en) | 2016-08-02 | 2025-03-25 | Hoffmann La Roche | Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids |
| US10793923B2 (en) | 2016-11-09 | 2020-10-06 | Roche Molecular Systems, Inc. | Compositions and methods for detection of BK virus |
| US10760137B2 (en) | 2017-07-18 | 2020-09-01 | Roche Molecular Systems, Inc. | Compositions and methods for detection of Babesia |
| WO2019063661A1 (en) | 2017-09-29 | 2019-04-04 | Roche Diagnostics Gmbh | COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS |
| US11535903B2 (en) | 2018-01-31 | 2022-12-27 | Juno Therapeutics, Inc. | Methods and reagents for assessing the presence or absence of replication competent virus |
| US11604186B2 (en) * | 2018-10-17 | 2023-03-14 | Molecular Devices (Austria) GmbH | Real time western blot assays utilizing fluorescence resonance energy transfer (FRET) |
| JP7478734B2 (ja) | 2018-12-03 | 2024-05-07 | エフ． ホフマン－ラ ロシュ アーゲー | カンジダ・アウリス（ｃａｎｄｉｄａ ａｕｒｉｓ）の検出のための組成物および方法 |
| JP7628085B2 (ja) | 2019-05-02 | 2025-02-07 | エフ． ホフマン－ラ ロシュ アーゲー | 鎖分離温度に基づくＤＮＡに対してＲＮＡ標的の優先的／選択的増幅のためのｄＩＴＰの利用 |
| WO2020225231A1 (en) | 2019-05-07 | 2020-11-12 | F. Hoffmann-La Roche Ag | Compositions and methods for detection of neisseria gonorroheae |
| WO2021013972A1 (en) | 2019-07-25 | 2021-01-28 | F. Hoffmann-La Roche Ag | Compositions and methods for detection of epstein barr virus (ebv) |
| JP7677950B2 (ja) | 2019-08-27 | 2025-05-15 | エフ． ホフマン－ラ ロシュ アーゲー | ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法 |
| US11441167B2 (en) | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
| ES2991814T3 (es) | 2019-12-27 | 2024-12-05 | Hoffmann La Roche | Composiciones y procedimientos para detectar Staphylococcus aureus resistente a la meticilina |
| US20220356537A1 (en) | 2019-12-31 | 2022-11-10 | Roche Molecular Systems, Inc. | Quantitative pcr screening of inducible prophage from bacterial isolates |
| CA3166337A1 (en) | 2020-03-09 | 2021-09-16 | F. Hoffmann-La Roche Ag | Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2), influenza a and influenza b |
| US20220042117A1 (en) | 2020-08-06 | 2022-02-10 | Roche Molecular Systems, Inc. | COMPOSITIONS AND METHODS FOR THE SIMULTANEOUS DETECTION OF INFLUENZA A, INFLUENZA B, AND SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2 (SARS-CoV-2) |
| CN112630198A (zh) * | 2020-09-15 | 2021-04-09 | 北京工业大学 | 一种基于回音壁模式光学微腔的传感检测方法 |
| EP4256089A1 (en) | 2020-12-04 | 2023-10-11 | F. Hoffmann-La Roche AG | Compositions and methods for detection of malaria |
| US20220205020A1 (en) | 2020-12-30 | 2022-06-30 | Roche Molecular Systems, Inc. | Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis |
| JP2024505686A (ja) | 2021-02-05 | 2024-02-07 | エフ． ホフマン－ラ ロシュ アーゲー | ヒトパラインフルエンザウイルス１－４（ｈｐｉｖ１－４）の検出のための組成物及び方法 |
| US20220290221A1 (en) | 2021-03-15 | 2022-09-15 | Roche Molecular Systems, Inc. | Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations |
| US20240240273A1 (en) | 2021-05-06 | 2024-07-18 | Roche Molecular Systems, Inc. | Compositions and Methods for Detecting Hepatitis Delta Virus by a Dual-Target Assay |
| CN118202069A (zh) | 2021-11-05 | 2024-06-14 | 豪夫迈·罗氏有限公司 | 用于检测疟疾的组合物和方法 |
| EP4437142A1 (en) | 2021-11-22 | 2024-10-02 | F. Hoffmann-La Roche AG | Compositions and methods for detecting vana and/or vanb genes associated with multidrug resistance |
| JP2025500496A (ja) | 2021-12-24 | 2025-01-09 | エフ． ホフマン－ラ ロシュ アーゲー | Ｌａｍｐ増幅産物の改良された検出 |
| US20260098309A1 (en) | 2022-06-30 | 2026-04-09 | Roche Molecular Systems, Inc. | Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis |
| US20260055475A1 (en) | 2022-08-24 | 2026-02-26 | Roche Molecular Systems, Inc. | Compositions and methods for detecting monkeypox virus |
| WO2024141442A1 (en) | 2022-12-25 | 2024-07-04 | F. Hoffmann-La Roche Ag | Compositions and methods for detecting carbapenem resistant acinetobacter calcoaceticus-baumannii (crab) |
| JP2026502046A (ja) | 2022-12-28 | 2026-01-21 | エフ． ホフマン－ラ ロシュ アーゲー | 複数のｂ型肝炎ウイルス遺伝子標的のためのデジタルｐｃｒアッセイ設計およびそのための非伸長性ブロッカーオリゴヌクレオチド |
| EP4669777A1 (en) | 2023-02-25 | 2025-12-31 | F. Hoffmann-La Roche AG | COMPOSITIONS AND METHODS FOR DETECTION OF CANDIDA SPECIES |
| EP4677114A1 (en) | 2023-03-03 | 2026-01-14 | F. Hoffmann-La Roche AG | Compositions and methods for the detection of group b streptococcus( streptococcus agalactiae) and clindamycin resistance gene determinants |
| CN121889512A (zh) | 2023-08-03 | 2026-04-17 | 罗氏测序解决方案公司 | 使用5'加尾长引物来改善靶向短引物结合位点时的扩增性能 |
| WO2025202488A1 (en) | 2024-03-28 | 2025-10-02 | Roche Diagnostics Gmbh | Enhancer of nucleic acid amplification |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4318846A (en) * | 1979-09-07 | 1982-03-09 | Syva Company | Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers |
| US4458066A (en) * | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| DE3071373D1 (en) | 1980-10-27 | 1986-03-06 | Syva Co | Novel ether substituted fluorescein compounds as fluorescers and quenchers |
| EP0144914A3 (en) | 1983-12-12 | 1986-08-13 | Miles Inc. | Hybridization assay employing labeled pairs of hybrid binding reagents |
| US5366860A (en) * | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
| US5188934A (en) * | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| EP0476545B1 (en) * | 1990-09-14 | 1997-05-07 | Tosoh Corporation | Method of and kit for immunoassay |
| EP1067134B1 (en) | 1991-11-07 | 2004-07-28 | Nanotronics, Inc. | Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system |
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| US5654419A (en) * | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| US6028190A (en) * | 1994-02-01 | 2000-02-22 | The Regents Of The University Of California | Probes labeled with energy transfer coupled dyes |
| WO1995030139A1 (en) * | 1994-04-29 | 1995-11-09 | Perkin-Elmer Corporation | System for real time detection of nucleic acid amplification products |
| US5863727A (en) * | 1996-05-03 | 1999-01-26 | The Perkin-Elmer Corporation | Energy transfer dyes with enhanced fluorescence |
| US5800996A (en) * | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
| US5945526A (en) * | 1996-05-03 | 1999-08-31 | Perkin-Elmer Corporation | Energy transfer dyes with enhanced fluorescence |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5853992A (en) * | 1996-10-04 | 1998-12-29 | The Regents Of The University Of California | Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels |
| US6365346B1 (en) * | 1998-02-18 | 2002-04-02 | Dade Behring Inc. | Quantitative determination of nucleic acid amplification products |
| US6768000B1 (en) * | 1998-06-12 | 2004-07-27 | Intergen Company | Multi-fluorescent hairpin energy transfer oligonucleotides |
| US6037130A (en) | 1998-07-28 | 2000-03-14 | The Public Health Institute Of The City Of New York, Inc. | Wavelength-shifting probes and primers and their use in assays and kits |
| US6610842B1 (en) * | 1999-05-06 | 2003-08-26 | Isis Pharmaceuticals, Inc. | Processes for the synthesis of oligomers using phosphoramidite compositions |
| US6524644B1 (en) | 1999-08-26 | 2003-02-25 | Enthone Inc. | Process for selective deposition of OSP coating on copper, excluding deposition on gold |
| US6114518A (en) * | 1999-09-30 | 2000-09-05 | Becton, Dickinson And Company | Synthesis and use of labelled phosphoramidite compositions |
| US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
| US6534646B2 (en) * | 2001-06-04 | 2003-03-18 | Barrskogen, Inc. | Oligonucleotide labeling reagents |
| US7297494B2 (en) * | 2001-06-25 | 2007-11-20 | Georgia Tech Research Corporation | Activatable probes and methods for in vivo gene detection |
| US20050164316A1 (en) * | 2001-06-27 | 2005-07-28 | Bray Terry L. | Biophotonic sensors and methods of use thereof |
| AU2002366046A1 (en) | 2001-10-19 | 2003-06-10 | Proligo Llc | Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes |
| US20040197793A1 (en) * | 2002-08-30 | 2004-10-07 | Arjang Hassibi | Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing |
| US20050118619A1 (en) * | 2003-09-22 | 2005-06-02 | Wensheng Xia | Dark quenchers for fluorescence resonance energy transfer (FRET) in bioassays |
| KR101244759B1 (ko) | 2004-06-18 | 2013-03-19 | 엑손모빌 업스트림 리서치 캄파니 | 확장형 용량의 액화 천연 가스 플랜트 |
-
2006
- 2006-10-05 WO PCT/EP2006/009646 patent/WO2007039301A2/en not_active Ceased
- 2006-10-05 US US11/544,460 patent/US7741467B2/en not_active Expired - Fee Related
- 2006-10-05 AT AT06806056T patent/ATE519863T1/de active
- 2006-10-05 ES ES06806056T patent/ES2371088T3/es active Active
- 2006-10-05 CN CN200680045298.1A patent/CN101321878B/zh not_active Expired - Fee Related
- 2006-10-05 EP EP06806056A patent/EP1934374B1/en not_active Not-in-force
- 2006-10-05 JP JP2008533937A patent/JP4843044B2/ja not_active Expired - Fee Related
- 2006-10-05 CA CA2624917A patent/CA2624917C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US7741467B2 (en) | 2010-06-22 |
| JP4843044B2 (ja) | 2011-12-21 |
| CN101321878B (zh) | 2013-03-27 |
| HK1125678A1 (en) | 2009-08-14 |
| ATE519863T1 (de) | 2011-08-15 |
| WO2007039301A2 (en) | 2007-04-12 |
| WO2007039301A3 (en) | 2007-05-24 |
| US20070077588A1 (en) | 2007-04-05 |
| EP1934374A2 (en) | 2008-06-25 |
| EP1934374B1 (en) | 2011-08-10 |
| CA2624917C (en) | 2013-07-09 |
| CN101321878A (zh) | 2008-12-10 |
| JP2009512837A (ja) | 2009-03-26 |
| CA2624917A1 (en) | 2007-04-12 |
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