ATE519863T1 - Nicht-fluoreszente energieübertragung - Google Patents
Nicht-fluoreszente energieübertragungInfo
- Publication number
- ATE519863T1 ATE519863T1 AT06806056T AT06806056T ATE519863T1 AT E519863 T1 ATE519863 T1 AT E519863T1 AT 06806056 T AT06806056 T AT 06806056T AT 06806056 T AT06806056 T AT 06806056T AT E519863 T1 ATE519863 T1 AT E519863T1
- Authority
- AT
- Austria
- Prior art keywords
- moieties
- donor
- fluorescent
- fluorescent energy
- biomolecules
- Prior art date
Links
- 238000000034 method Methods 0.000 abstract 2
- 239000011541 reaction mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Luminescent Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US72420205P | 2005-10-05 | 2005-10-05 | |
| PCT/EP2006/009646 WO2007039301A2 (en) | 2005-10-05 | 2006-10-05 | Non-fluorescent energy transfer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE519863T1 true ATE519863T1 (de) | 2011-08-15 |
Family
ID=37574999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06806056T ATE519863T1 (de) | 2005-10-05 | 2006-10-05 | Nicht-fluoreszente energieübertragung |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7741467B2 (de) |
| EP (1) | EP1934374B1 (de) |
| JP (1) | JP4843044B2 (de) |
| CN (1) | CN101321878B (de) |
| AT (1) | ATE519863T1 (de) |
| CA (1) | CA2624917C (de) |
| ES (1) | ES2371088T3 (de) |
| WO (1) | WO2007039301A2 (de) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| FI20070163A0 (fi) * | 2007-02-27 | 2007-02-27 | Hidex Oy | Parannettu homogeeninen luminesenssibiotesti |
| JP2010525333A (ja) * | 2007-04-18 | 2010-07-22 | ベクトン・ディキンソン・アンド・カンパニー | Ｓｅｒｓナノタグを用いる分析法 |
| US8206929B2 (en) * | 2009-01-07 | 2012-06-26 | Roche Molecular Systems, Inc. | Nucleic acid amplification with allele-specific suppression of sequence variants |
| CA2753269C (en) * | 2009-02-20 | 2017-04-25 | Perkinelmer Biosignal, Inc. | Multiplex assay methods and compositions |
| GB2480792B (en) * | 2009-03-02 | 2018-11-14 | Co Diagnostics Inc | Energy transfer hairpin oligonucleotide probes |
| US8674080B2 (en) | 2009-04-09 | 2014-03-18 | Roche Molecular Systems, Inc. | Dye composition for liquid transfer control |
| WO2016065207A1 (en) | 2014-10-23 | 2016-04-28 | Ricardo Mancebo | Reagents and methods for isothermal chain reaction |
| US9745618B2 (en) | 2014-11-19 | 2017-08-29 | Roche Molecular Systems, Inc. | Photoblocked probes and methods for sequential detection of nucleic acids |
| US9920381B2 (en) | 2014-12-02 | 2018-03-20 | Roche Molecular Systems, Inc. | Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus |
| EP3426805B1 (de) | 2016-03-11 | 2023-11-29 | Roche Diagnostics GmbH | Zusammensetzungen und verfahren zum nachweis des zikavirus |
| US10612101B2 (en) | 2016-05-27 | 2020-04-07 | Roche Molecular Systems, Inc. | Compositions and methods for detection of Mycoplasma genitalium |
| WO2017202894A1 (en) | 2016-05-27 | 2017-11-30 | Roche Diagnostics Gmbh | Compositions and methods for detection of trichomonas vaginalis |
| CN109496378A (zh) * | 2016-06-03 | 2019-03-19 | 通用医疗公司 | 用于微激光器粒子的系统和方法 |
| US10781175B2 (en) | 2016-07-15 | 2020-09-22 | Am Chemicals Llc | Solid supports and phosphoramidite building blocks for oligonucleotide conjugates |
| MX2019001186A (es) | 2016-07-29 | 2019-08-29 | Juno Therapeutics Inc | Metodos para evaluar la presencia o la ausencia de un virus capaz de replicacion. |
| ES3008759T3 (en) | 2016-08-02 | 2025-03-25 | Hoffmann La Roche | Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids |
| US10793923B2 (en) | 2016-11-09 | 2020-10-06 | Roche Molecular Systems, Inc. | Compositions and methods for detection of BK virus |
| US10760137B2 (en) | 2017-07-18 | 2020-09-01 | Roche Molecular Systems, Inc. | Compositions and methods for detection of Babesia |
| WO2019063661A1 (en) | 2017-09-29 | 2019-04-04 | Roche Diagnostics Gmbh | COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS |
| US11535903B2 (en) | 2018-01-31 | 2022-12-27 | Juno Therapeutics, Inc. | Methods and reagents for assessing the presence or absence of replication competent virus |
| US11604186B2 (en) * | 2018-10-17 | 2023-03-14 | Molecular Devices (Austria) GmbH | Real time western blot assays utilizing fluorescence resonance energy transfer (FRET) |
| JP7478734B2 (ja) | 2018-12-03 | 2024-05-07 | エフ． ホフマン－ラ ロシュ アーゲー | カンジダ・アウリス（ｃａｎｄｉｄａ ａｕｒｉｓ）の検出のための組成物および方法 |
| EP3963102A1 (de) | 2019-05-02 | 2022-03-09 | F. Hoffmann-La Roche AG | Nutzung von ditp zur bevorzugten/selektiven amplifikation von rna gegenüber dna-zielen basierend auf der strangtrenntemperatur |
| WO2020225231A1 (en) | 2019-05-07 | 2020-11-12 | F. Hoffmann-La Roche Ag | Compositions and methods for detection of neisseria gonorroheae |
| ES2988562T3 (es) | 2019-07-25 | 2024-11-20 | Hoffmann La Roche | Composiciones y procedimientos para la detección del virus de Epstein-Barr (VEB) |
| CN114286866A (zh) | 2019-08-27 | 2022-04-05 | 豪夫迈·罗氏有限公司 | 用于扩增和检测包括从cccDNA转录的HBV RNA在内的乙型肝炎病毒RNA的组合物和方法 |
| US11441167B2 (en) | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
| CN114867871A (zh) | 2019-12-27 | 2022-08-05 | 豪夫迈·罗氏有限公司 | 用于检测耐甲氧西林金黄色葡萄球菌的组合物和方法 |
| US20220356537A1 (en) | 2019-12-31 | 2022-11-10 | Roche Molecular Systems, Inc. | Quantitative pcr screening of inducible prophage from bacterial isolates |
| WO2021180631A1 (en) | 2020-03-09 | 2021-09-16 | F. Hoffmann-La Roche Ag | Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2), influenza a and influenza b |
| WO2022029157A2 (en) | 2020-08-06 | 2022-02-10 | F. Hoffmann-La Roche Ag | Compositions and methods for the detection of severe acute respiratory syndrome coronavirus 2 (sars-2), influenza a, and influenza b |
| CN112630198A (zh) * | 2020-09-15 | 2021-04-09 | 北京工业大学 | 一种基于回音壁模式光学微腔的传感检测方法 |
| EP4256089A1 (de) | 2020-12-04 | 2023-10-11 | F. Hoffmann-La Roche AG | Zusammensetzungen und verfahren zum nachweis von malaria |
| US20220205020A1 (en) | 2020-12-30 | 2022-06-30 | Roche Molecular Systems, Inc. | Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis |
| WO2022167570A1 (en) | 2021-02-05 | 2022-08-11 | F. Hoffmann-La Roche Ag | Compositions and methods for detection of human parainfluenza viruses 1-4 (hpiv 1-4) |
| US20220290221A1 (en) | 2021-03-15 | 2022-09-15 | Roche Molecular Systems, Inc. | Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations |
| JP2024517835A (ja) | 2021-05-06 | 2024-04-23 | エフ． ホフマン－ラ ロシュ アーゲー | 二重標的アッセイによりデルタ型肝炎ウイルスを検出するための組成物及び方法 |
| WO2023079032A1 (en) | 2021-11-05 | 2023-05-11 | F. Hoffmann-La Roche Ag | Compositions and methods for detection of malaria |
| US20250011882A1 (en) | 2021-11-22 | 2025-01-09 | Roche Molecular Systems, Inc. | Compositions and Methods for Detecting vanA and/or vanB Genes Associated with Multidrug Resistance |
| US20250382664A1 (en) | 2021-12-24 | 2025-12-18 | Roche Molecular Systems, Inc. | Improved detection of lamp amplification products |
| JP2025521750A (ja) | 2022-06-30 | 2025-07-10 | エフ． ホフマン－ラ ロシュ アーゲー | クラミジア・トラコマチス（ｃｈｌａｍｙｄｉａ ｔｒａｃｈｏｍａｔｉｓ）の鼠径リンパ肉芽腫症（ｌｇｖ）血清型を検出するための組成物および方法 |
| WO2024042042A1 (en) | 2022-08-24 | 2024-02-29 | F. Hoffmann-La Roche Ag | Compositions and methods for detecting monkeypox virus |
| CN120418449A (zh) | 2022-12-25 | 2025-08-01 | 豪夫迈·罗氏有限公司 | 用于检测耐碳青霉烯的醋酸钙不动杆菌-鲍氏不动杆菌(crab)的组合物和方法 |
| WO2024141476A1 (en) | 2022-12-28 | 2024-07-04 | F. Hoffmann-La Roche Ag | Digital pcr assay designs for multiple hepatitis b virus gene targets and non-extendable blocker oligonucleotides therefor |
| WO2024175749A1 (en) | 2023-02-25 | 2024-08-29 | F. Hoffmann-La Roche Ag | Compositions and methods for candida species detection |
| CN120917150A (zh) | 2023-03-03 | 2025-11-07 | 豪夫迈·罗氏有限公司 | 用于检测b族链球菌（无乳链球菌）和克林霉素抗性基因决定子的组合物和方法 |
| WO2025027080A1 (en) | 2023-08-03 | 2025-02-06 | F. Hoffmann-La Roche Ag | Use of 5'-tailed long primers to improve amplification performance when targeting short primer-binding sites |
| WO2025202488A1 (en) | 2024-03-28 | 2025-10-02 | Roche Diagnostics Gmbh | Enhancer of nucleic acid amplification |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4318846A (en) | 1979-09-07 | 1982-03-09 | Syva Company | Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| DE3071373D1 (en) | 1980-10-27 | 1986-03-06 | Syva Co | Novel ether substituted fluorescein compounds as fluorescers and quenchers |
| EP0144914A3 (de) | 1983-12-12 | 1986-08-13 | Miles Inc. | Hybridisierungstest mittels markierter Paare von hybridbindenden Reagenzien |
| US5366860A (en) | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
| US5188934A (en) | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| EP0476545B1 (de) | 1990-09-14 | 1997-05-07 | Tosoh Corporation | Verfahren und Kit für Immunoassay |
| ATE272068T1 (de) * | 1991-11-07 | 2004-08-15 | Nanotronics Inc | Hybridisierung von mit chromophoren und fluorophoren konjugierten polynukleotiden zur erzeugung eines donor-donor energietransfersystems |
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| US6028190A (en) | 1994-02-01 | 2000-02-22 | The Regents Of The University Of California | Probes labeled with energy transfer coupled dyes |
| US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| ATE198511T1 (de) | 1994-04-29 | 2001-01-15 | Perkin Elmer Corp | Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation |
| US5800996A (en) | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
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| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5853992A (en) * | 1996-10-04 | 1998-12-29 | The Regents Of The University Of California | Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels |
| US6365346B1 (en) | 1998-02-18 | 2002-04-02 | Dade Behring Inc. | Quantitative determination of nucleic acid amplification products |
| US6768000B1 (en) | 1998-06-12 | 2004-07-27 | Intergen Company | Multi-fluorescent hairpin energy transfer oligonucleotides |
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| US6524644B1 (en) | 1999-08-26 | 2003-02-25 | Enthone Inc. | Process for selective deposition of OSP coating on copper, excluding deposition on gold |
| US6114518A (en) | 1999-09-30 | 2000-09-05 | Becton, Dickinson And Company | Synthesis and use of labelled phosphoramidite compositions |
| US7019129B1 (en) * | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
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| US7297494B2 (en) * | 2001-06-25 | 2007-11-20 | Georgia Tech Research Corporation | Activatable probes and methods for in vivo gene detection |
| AU2002327184A1 (en) * | 2001-06-27 | 2003-03-03 | The Uab Research Foundation | Biophotonic sensors and methods of use thereof |
| EP1442142A4 (de) | 2001-10-19 | 2006-11-15 | Proligo Llc | Nukleinsäuresonden und verfahren zum nachweis und zur quantifizierung von nukleinsäureanalyten |
| US20040197793A1 (en) | 2002-08-30 | 2004-10-07 | Arjang Hassibi | Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing |
| US20050118619A1 (en) * | 2003-09-22 | 2005-06-02 | Wensheng Xia | Dark quenchers for fluorescence resonance energy transfer (FRET) in bioassays |
| CA2570835C (en) | 2004-06-18 | 2013-10-22 | Exxonmobil Upstream Research Company | Scalable capacity liquefied natural gas plant |
-
2006
- 2006-10-05 CN CN200680045298.1A patent/CN101321878B/zh not_active Expired - Fee Related
- 2006-10-05 AT AT06806056T patent/ATE519863T1/de active
- 2006-10-05 EP EP06806056A patent/EP1934374B1/de not_active Not-in-force
- 2006-10-05 JP JP2008533937A patent/JP4843044B2/ja not_active Expired - Fee Related
- 2006-10-05 US US11/544,460 patent/US7741467B2/en not_active Expired - Fee Related
- 2006-10-05 WO PCT/EP2006/009646 patent/WO2007039301A2/en not_active Ceased
- 2006-10-05 ES ES06806056T patent/ES2371088T3/es active Active
- 2006-10-05 CA CA2624917A patent/CA2624917C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1934374A2 (de) | 2008-06-25 |
| EP1934374B1 (de) | 2011-08-10 |
| CA2624917A1 (en) | 2007-04-12 |
| WO2007039301A3 (en) | 2007-05-24 |
| CN101321878A (zh) | 2008-12-10 |
| US20070077588A1 (en) | 2007-04-05 |
| CA2624917C (en) | 2013-07-09 |
| JP4843044B2 (ja) | 2011-12-21 |
| CN101321878B (zh) | 2013-03-27 |
| US7741467B2 (en) | 2010-06-22 |
| WO2007039301A2 (en) | 2007-04-12 |
| HK1125678A1 (en) | 2009-08-14 |
| ES2371088T3 (es) | 2011-12-27 |
| JP2009512837A (ja) | 2009-03-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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