ES2305536T3 - Proteinas g alfa q-gustducina quimericas. - Google Patents
Proteinas g alfa q-gustducina quimericas. Download PDFInfo
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- ES2305536T3 ES2305536T3 ES03776743T ES03776743T ES2305536T3 ES 2305536 T3 ES2305536 T3 ES 2305536T3 ES 03776743 T ES03776743 T ES 03776743T ES 03776743 T ES03776743 T ES 03776743T ES 2305536 T3 ES2305536 T3 ES 2305536T3
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4722—G-proteins
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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| US43479002P | 2002-12-18 | 2002-12-18 | |
| US434790P | 2002-12-18 |
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| ES2305536T3 true ES2305536T3 (es) | 2008-11-01 |
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| ES03776743T Expired - Lifetime ES2305536T3 (es) | 2002-12-18 | 2003-12-17 | Proteinas g alfa q-gustducina quimericas. |
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| EP (1) | EP1581555B1 (enExample) |
| JP (2) | JP4654038B2 (enExample) |
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| AT (1) | ATE393778T1 (enExample) |
| AU (1) | AU2003286069A1 (enExample) |
| CA (1) | CA2507387A1 (enExample) |
| DE (1) | DE60320682T2 (enExample) |
| ES (1) | ES2305536T3 (enExample) |
| WO (1) | WO2004055048A2 (enExample) |
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| US7368285B2 (en) | 2001-03-07 | 2008-05-06 | Senomyx, Inc. | Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same |
| DK2327985T3 (en) | 2001-06-26 | 2016-09-05 | Senomyx Inc | H1 Oligomeric T1R Taste Receptors and Cell Lines Expressing the Receptors, and Their Use to Identify Taste Compounds |
| WO2004055048A2 (en) * | 2002-12-18 | 2004-07-01 | Givaudan Sa | Chimeric alpha q-gustducin g-proteins |
| BRPI0518075B8 (pt) | 2004-10-29 | 2017-05-16 | Quest Int Services B V | composição flavorizante, produto, e, processo de melhorar o flavor de um alimento, de uma bebida ou de um produto farmacêutico |
| ATE535508T1 (de) | 2004-10-29 | 2011-12-15 | Givaudan Nederland Services Bv | Geschmacksmodulierende substanzen |
| EP1759595B1 (en) | 2005-09-02 | 2009-08-05 | Givaudan Nederland Services B.V. | Flavour modulating substance |
| US8124360B2 (en) | 2006-04-20 | 2012-02-28 | Givaudan S.A. | Use of a T1R2 nucleic acid sequence to identify tastants |
| US20110097741A1 (en) * | 2006-04-20 | 2011-04-28 | Jay Patrick Slack | Partial t1r2 nucleic acid sequence, receptor protein and its use in screening assays |
| KR101356914B1 (ko) | 2006-04-20 | 2014-01-28 | 지보당 에스아 | 단맛 강화 방법 |
| PL1854782T3 (pl) | 2006-05-05 | 2010-02-26 | Givaudan Nederland Services Bv | Kompozycja ulepszająca smak |
| KR101511394B1 (ko) * | 2007-01-18 | 2015-04-10 | 지보당 에스아 | 키메라 우마미 미각 수용체, 이를 코딩하는 핵산 및 이의 용도 |
| EP1975611A1 (en) | 2007-03-29 | 2008-10-01 | Deutsches Institut für Ernährungsforschung Potsdam -Rehbrücke-Stiftung des öffentlichen Rechts- Vertreten durch den Stiftungsvorstand | Agonists of bitter taste receptors and uses thereof |
| US8187822B2 (en) | 2007-03-30 | 2012-05-29 | Givaudan S.A. | Methods to identify modulators |
| WO2009076428A1 (en) * | 2007-12-10 | 2009-06-18 | Metaproteomics, Llc | Substituted 1,3-cyclopentadione multi-target protein kinase modulators of cancer, angiogenesis and the inflammatory pathways associated therewith |
| EP2108954A1 (en) | 2008-04-10 | 2009-10-14 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | A test system for detecting intracellular cyclic nucleotide level and methods and uses thereof |
| WO2009149577A1 (en) * | 2008-06-13 | 2009-12-17 | Givaudan Sa | Methods of identifying modulators of the bitter taste receptor tas2r44 |
| JP5576653B2 (ja) * | 2008-12-26 | 2014-08-20 | サントリー食品インターナショナル株式会社 | 環状ジペプチド含有飲料 |
| EP2924112A1 (en) * | 2009-02-02 | 2015-09-30 | Chromocell Corporation | Novel cell lines and methods |
| EP2567229A1 (en) | 2010-05-07 | 2013-03-13 | Givaudan SA | Methods to identify modulators |
| US20130123135A1 (en) | 2010-05-07 | 2013-05-16 | Jay Patrick Slack | Methods To Identify Modulators of TAS2R48 Receptors |
| JP5906013B2 (ja) * | 2010-12-10 | 2016-04-20 | 花王株式会社 | 苦味抑制剤の評価・選択方法 |
| WO2013103384A1 (en) | 2012-01-06 | 2013-07-11 | Elcelyx Therapeutics, Inc. | Biguanide compositions and methods of treating metabolic disorders |
| WO2012094636A2 (en) | 2011-01-07 | 2012-07-12 | Elcelyx Therapeutics, Inc. | Chemosensory receptor ligand-based therapies |
| US9795792B2 (en) | 2011-02-25 | 2017-10-24 | Medtronic, Inc. | Emergency mode switching for non-pacing modes |
| WO2013022948A1 (en) | 2011-08-08 | 2013-02-14 | The Coca-Cola Company | Cell lines comprising endogenous taste receptors and their uses |
| WO2013072332A1 (en) | 2011-11-14 | 2013-05-23 | Givaudan Sa | Methods of using antagonists of bitter taste receptors |
| SG10201901922VA (en) | 2012-01-06 | 2019-04-29 | Elcelyx Therapeutics Inc | Compositions and methods for treating metabolic disorders |
| AU2013290100A1 (en) | 2012-07-11 | 2015-01-29 | Elcelyx Therapeutics, Inc. | Compositions comprising statins, biguanides and further agents for reducing cardiometabolic risk |
| CN110051638B (zh) | 2013-01-05 | 2022-02-01 | 安济药业(美国)有限责任公司 | 包含双胍的延迟释放组合物 |
| CA2903208C (en) | 2013-03-15 | 2021-05-18 | Applied Food Biotechnology, Inc. | Feline bitter taste receptors and methods |
| US9804157B1 (en) | 2013-03-15 | 2017-10-31 | Senomyx, Inc. | Screening assays to identify compounds which modulate T1R associated taste modalities which eliminate false positives |
| US9766236B2 (en) | 2013-05-10 | 2017-09-19 | Pepsico, Inc. | Taste receptor internalization assay |
| US10107794B2 (en) | 2013-05-10 | 2018-10-23 | Pepsico, Inc. | Methods of using U2-OS cells to identify potential sweet taste modulators |
| JP6755640B2 (ja) | 2014-03-28 | 2020-09-16 | 味の素株式会社 | 甘味受容体キメラタンパク質及びその利用 |
| WO2017066799A1 (en) * | 2015-10-16 | 2017-04-20 | Cornell University | Sweeteners having enhanced organoleptic properties |
| JP6924590B2 (ja) * | 2016-03-14 | 2021-08-25 | サントリーホールディングス株式会社 | 苦味評価のための融合タンパク質および苦味評価方法 |
| CN108611324A (zh) * | 2018-05-07 | 2018-10-02 | 云南中烟工业有限责任公司 | 一种稳定共表达重组人源甜味受体蛋白的真核细胞系及其制备方法 |
| WO2025183058A1 (ja) * | 2024-02-29 | 2025-09-04 | 味の素株式会社 | 植物由来タンパク質含有食品における異味・異風味をマスキングする物質のスクリーニング方法および異味・異風味のマスキング用の組成物 |
| CN119193656A (zh) * | 2024-09-24 | 2024-12-27 | 上海交通大学 | 一种响应鲜味信号的酵母细胞模型及其制备方法与应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5817759A (en) * | 1992-04-09 | 1998-10-06 | Linguagen Corporation | Gustducin polypeptides and fragments |
| CN1289525C (zh) | 1999-09-10 | 2006-12-13 | 加利福尼亚大学董事会 | T2r,一类味觉受体 |
| US6462178B1 (en) * | 1999-11-17 | 2002-10-08 | Yong Hou Wong | G protein |
| US7041457B2 (en) * | 2000-10-30 | 2006-05-09 | Senomyx, Inc. | Gαq protein variants and their use in the analysis and discovery of agonists and antagonists of chemosensory receptors |
| AU2002213409A1 (en) | 2000-10-30 | 2002-05-15 | Senomyx, Inc. | Galphaqproetin variants and their use in the analysis and discovery of agonists and antagonists of chemosensory receptors |
| WO2004055048A2 (en) * | 2002-12-18 | 2004-07-01 | Givaudan Sa | Chimeric alpha q-gustducin g-proteins |
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2003
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- 2003-12-17 AT AT03776743T patent/ATE393778T1/de not_active IP Right Cessation
- 2003-12-17 ES ES03776743T patent/ES2305536T3/es not_active Expired - Lifetime
- 2003-12-17 DE DE60320682T patent/DE60320682T2/de not_active Expired - Lifetime
- 2003-12-17 CN CNA2003801065075A patent/CN1726224A/zh active Pending
- 2003-12-17 EP EP03776743A patent/EP1581555B1/en not_active Expired - Lifetime
- 2003-12-17 AU AU2003286069A patent/AU2003286069A1/en not_active Abandoned
- 2003-12-17 JP JP2004559553A patent/JP4654038B2/ja not_active Expired - Lifetime
- 2003-12-17 US US10/538,038 patent/US7919236B2/en not_active Expired - Lifetime
- 2003-12-17 CA CA002507387A patent/CA2507387A1/en not_active Abandoned
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| DE60320682T2 (de) | 2009-06-10 |
| AU2003286069A1 (en) | 2004-07-09 |
| WO2004055048A2 (en) | 2004-07-01 |
| AU2003286069A8 (en) | 2004-07-09 |
| CN1726224A (zh) | 2006-01-25 |
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| US8609362B2 (en) | 2013-12-17 |
| JP2010187666A (ja) | 2010-09-02 |
| DE60320682D1 (de) | 2008-06-12 |
| CA2507387A1 (en) | 2004-07-01 |
| JP4654038B2 (ja) | 2011-03-16 |
| EP1581555A2 (en) | 2005-10-05 |
| ATE393778T1 (de) | 2008-05-15 |
| US7919236B2 (en) | 2011-04-05 |
| US20060275765A1 (en) | 2006-12-07 |
| EP1581555B1 (en) | 2008-04-30 |
| JP5425651B2 (ja) | 2014-02-26 |
| US20110177545A1 (en) | 2011-07-21 |
| JP2006524483A (ja) | 2006-11-02 |
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