ES2304971T3 - Metodo para mediciones de electroluminiscencia. - Google Patents
Metodo para mediciones de electroluminiscencia. Download PDFInfo
- Publication number
- ES2304971T3 ES2304971T3 ES00953206T ES00953206T ES2304971T3 ES 2304971 T3 ES2304971 T3 ES 2304971T3 ES 00953206 T ES00953206 T ES 00953206T ES 00953206 T ES00953206 T ES 00953206T ES 2304971 T3 ES2304971 T3 ES 2304971T3
- Authority
- ES
- Spain
- Prior art keywords
- luminescence
- electrically conductive
- electrodes
- metal
- luminescent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 53
- 238000005259 measurement Methods 0.000 title description 5
- 239000004020 conductor Substances 0.000 claims abstract description 78
- 150000001875 compounds Chemical class 0.000 claims abstract description 39
- 239000003792 electrolyte Substances 0.000 claims abstract description 31
- 238000004020 luminiscence type Methods 0.000 claims description 49
- 229910052751 metal Inorganic materials 0.000 claims description 30
- 239000002184 metal Substances 0.000 claims description 30
- 239000000463 material Substances 0.000 claims description 22
- 239000010931 gold Substances 0.000 claims description 15
- 239000012491 analyte Substances 0.000 claims description 10
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 10
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 9
- 229910052737 gold Inorganic materials 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- 229910052782 aluminium Inorganic materials 0.000 claims description 8
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 8
- 229910052697 platinum Inorganic materials 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 4
- 238000011002 quantification Methods 0.000 claims description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 3
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 3
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 claims description 3
- 229910052735 hafnium Inorganic materials 0.000 claims description 3
- VBJZVLUMGGDVMO-UHFFFAOYSA-N hafnium atom Chemical compound [Hf] VBJZVLUMGGDVMO-UHFFFAOYSA-N 0.000 claims description 3
- 229910052749 magnesium Inorganic materials 0.000 claims description 3
- 239000011777 magnesium Substances 0.000 claims description 3
- 239000011255 nonaqueous electrolyte Substances 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 229910052715 tantalum Inorganic materials 0.000 claims description 3
- GUVRBAGPIYLISA-UHFFFAOYSA-N tantalum atom Chemical compound [Ta] GUVRBAGPIYLISA-UHFFFAOYSA-N 0.000 claims description 3
- 229910052719 titanium Inorganic materials 0.000 claims description 3
- 239000010936 titanium Substances 0.000 claims description 3
- 229910052726 zirconium Inorganic materials 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 239000006181 electrochemical material Substances 0.000 claims description 2
- 229910021397 glassy carbon Inorganic materials 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 239000000696 magnetic material Substances 0.000 claims 1
- 238000001378 electrochemiluminescence detection Methods 0.000 description 46
- 230000005284 excitation Effects 0.000 description 20
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 18
- 230000004044 response Effects 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 10
- 239000012488 sample solution Substances 0.000 description 10
- 239000000872 buffer Substances 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 230000035945 sensitivity Effects 0.000 description 8
- 239000000427 antigen Substances 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 238000003018 immunoassay Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000006249 magnetic particle Substances 0.000 description 6
- HKCRVXUAKWXBLE-UHFFFAOYSA-N terbium(3+) Chemical class [Tb+3] HKCRVXUAKWXBLE-UHFFFAOYSA-N 0.000 description 6
- 239000000956 alloy Substances 0.000 description 5
- 229910045601 alloy Inorganic materials 0.000 description 5
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 5
- 150000002739 metals Chemical class 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- GZCRRIHWUXGPOV-UHFFFAOYSA-N terbium atom Chemical compound [Tb] GZCRRIHWUXGPOV-UHFFFAOYSA-N 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- -1 indian Chemical compound 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 3
- 229910052771 Terbium Inorganic materials 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000003111 delayed effect Effects 0.000 description 3
- 239000008151 electrolyte solution Substances 0.000 description 3
- 229940021013 electrolyte solution Drugs 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- JRKICGRDRMAZLK-UHFFFAOYSA-L peroxydisulfate Chemical compound [O-]S(=O)(=O)OOS([O-])(=O)=O JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 description 3
- 229920000136 polysorbate Polymers 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- QPCDCPDFJACHGM-UHFFFAOYSA-N N,N-bis{2-[bis(carboxymethyl)amino]ethyl}glycine Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CC(O)=O)CC(O)=O QPCDCPDFJACHGM-UHFFFAOYSA-N 0.000 description 2
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 2
- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 102000011923 Thyrotropin Human genes 0.000 description 2
- 108010061174 Thyrotropin Proteins 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000013522 chelant Substances 0.000 description 2
- 238000003996 delayed luminescence Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 238000005868 electrolysis reaction Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000036046 immunoreaction Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 150000002894 organic compounds Chemical class 0.000 description 2
- 229910052762 osmium Inorganic materials 0.000 description 2
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 238000000159 protein binding assay Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229910052707 ruthenium Inorganic materials 0.000 description 2
- YFTHZRPMJXBUME-UHFFFAOYSA-N tripropylamine Chemical compound CCCN(CCC)CCC YFTHZRPMJXBUME-UHFFFAOYSA-N 0.000 description 2
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- HQELAEJMRRISNS-UHFFFAOYSA-N 2-[[3-[[bis(carboxymethyl)amino]methyl]-2-hydroxy-5-(3-isothiocyanatobenzoyl)phenyl]methyl-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)CC1=C(O)C(CN(CC(O)=O)CC(=O)O)=CC(C(=O)C=2C=C(C=CC=2)N=C=S)=C1 HQELAEJMRRISNS-UHFFFAOYSA-N 0.000 description 1
- AAVKRHMDXDMJKA-UHFFFAOYSA-N 2-[[5-benzoyl-3-[[bis(carboxymethyl)amino]methyl]-2-hydroxyphenyl]methyl-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)CC1=C(O)C(CN(CC(O)=O)CC(=O)O)=CC(C(=O)C=2C=CC=CC=2)=C1 AAVKRHMDXDMJKA-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- SHKFCYMWLOQJDW-UHFFFAOYSA-K [O-]C(CN(CC([O-])=O)CC(C=C(C=C1CN(CC([O-])=O)CC(O)=O)C(C2=CC=CC(N=C=S)=C2)=O)=C1O)=O.[Tb+3] Chemical compound [O-]C(CN(CC([O-])=O)CC(C=C(C=C1CN(CC([O-])=O)CC(O)=O)C(C2=CC=CC(N=C=S)=C2)=O)=C1O)=O.[Tb+3] SHKFCYMWLOQJDW-UHFFFAOYSA-K 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 150000001540 azides Chemical class 0.000 description 1
- 238000009739 binding Methods 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-M dihydrogenphosphate Chemical compound OP(O)([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-M 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005401 electroluminescence Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000004401 flow injection analysis Methods 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910001410 inorganic ion Inorganic materials 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 229910052741 iridium Inorganic materials 0.000 description 1
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 229910001004 magnetic alloy Inorganic materials 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000004081 narcotic agent Substances 0.000 description 1
- 230000036963 noncompetitive effect Effects 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 125000006353 oxyethylene group Chemical group 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052763 palladium Inorganic materials 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 229960003330 pentetic acid Drugs 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000011253 protective coating Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 229910052702 rhenium Inorganic materials 0.000 description 1
- WUAPFZMCVAUBPE-UHFFFAOYSA-N rhenium atom Chemical compound [Re] WUAPFZMCVAUBPE-UHFFFAOYSA-N 0.000 description 1
- 229910052703 rhodium Inorganic materials 0.000 description 1
- 239000010948 rhodium Substances 0.000 description 1
- MHOVAHRLVXNVSD-UHFFFAOYSA-N rhodium atom Chemical compound [Rh] MHOVAHRLVXNVSD-UHFFFAOYSA-N 0.000 description 1
- BPEVHDGLPIIAGH-UHFFFAOYSA-N ruthenium(3+) Chemical compound [Ru+3] BPEVHDGLPIIAGH-UHFFFAOYSA-N 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229910052713 technetium Inorganic materials 0.000 description 1
- GKLVYJBZJHMRIY-UHFFFAOYSA-N technetium atom Chemical compound [Tc] GKLVYJBZJHMRIY-UHFFFAOYSA-N 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000004454 trace mineral analysis Methods 0.000 description 1
- 229910052723 transition metal Inorganic materials 0.000 description 1
- 150000003624 transition metals Chemical class 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/66—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence
- G01N21/69—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence specially adapted for fluids, e.g. molten metal
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Steroid Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US376139 | 1999-08-17 | ||
| US09/376,139 US6136268A (en) | 1999-08-17 | 1999-08-17 | Method for luminescence measurements |
| PCT/FI2000/000693 WO2001013095A1 (en) | 1999-08-17 | 2000-08-16 | Method for luminescence measurements |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2304971T3 true ES2304971T3 (es) | 2008-11-01 |
Family
ID=23483870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES00953206T Expired - Lifetime ES2304971T3 (es) | 1999-08-17 | 2000-08-16 | Metodo para mediciones de electroluminiscencia. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6136268A (enExample) |
| EP (1) | EP1204858B1 (enExample) |
| JP (1) | JP4573486B2 (enExample) |
| AT (1) | ATE394663T1 (enExample) |
| AU (1) | AU777936B2 (enExample) |
| CA (1) | CA2380655A1 (enExample) |
| DE (1) | DE60038803D1 (enExample) |
| DK (1) | DK1204858T3 (enExample) |
| ES (1) | ES2304971T3 (enExample) |
| PT (1) | PT1204858E (enExample) |
| WO (1) | WO2001013095A1 (enExample) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6200531B1 (en) * | 1998-05-11 | 2001-03-13 | Igen International, Inc. | Apparatus for carrying out electrochemiluminescence test measurements |
| US6347708B1 (en) * | 1999-03-22 | 2002-02-19 | Cedarapids Inc. | Wheel case for a vibratory apparatus |
| DK2461156T3 (da) | 2001-06-29 | 2020-08-03 | Meso Scale Technologies Llc | Indretning til luminescenstestmålinger |
| CA2353120A1 (en) * | 2001-07-16 | 2003-01-16 | Cardiogenics Inc. | Caged compound cleaving process |
| EP3021106B8 (en) * | 2001-07-30 | 2019-03-27 | Meso Scale Technologies, LLC | Methods of using assay electrode having immobilized lipid/protein layers for luminescence test measurements |
| EP1436620B1 (en) | 2001-09-10 | 2014-04-23 | Meso Scale Technologies, LLC | Methods and apparatus for conducting multiple measurements on a sample |
| CA2783186C (en) * | 2001-09-10 | 2016-08-23 | Meso Scale Technologies, Llc | Assay buffer, compositions containing the same, and methods of using the same |
| FI118611B (fi) * | 2002-04-15 | 2008-01-15 | Labmaster Oy | Eriste-elektrodilaitteet |
| WO2004001380A2 (en) * | 2002-06-20 | 2003-12-31 | Igen International, Inc | Electrochemiluminescence flow cell and flow cell components |
| WO2004003219A2 (en) * | 2002-06-28 | 2004-01-08 | Igen International, Inc. | Improved assay systems and components |
| WO2004070081A2 (en) * | 2003-01-31 | 2004-08-19 | Envirofuels L.P. | Method and composition for creation of conversion surface |
| WO2004113891A1 (en) * | 2003-06-13 | 2004-12-29 | Applera Corporation | Electrochemiluminescence electrode |
| US7238536B1 (en) * | 2004-03-22 | 2007-07-03 | Florida State University Research Foundation, Inc. | Controlled transport through multiple reversible interaction point membranes |
| US7796266B2 (en) | 2004-04-30 | 2010-09-14 | Kimberly-Clark Worldwide, Inc. | Optical detection system using electromagnetic radiation to detect presence or quantity of analyte |
| US7815854B2 (en) * | 2004-04-30 | 2010-10-19 | Kimberly-Clark Worldwide, Inc. | Electroluminescent illumination source for optical detection systems |
| AU2005326758B2 (en) | 2004-06-23 | 2011-07-14 | Board Of Regents Of The University Of Texas System | Methods and compositions for the detection of biological molecules using a two particle complex |
| FI119894B (fi) * | 2005-03-30 | 2009-04-30 | Labmaster Oy | Elektrokemiluminesenssiin perustuva analyysimenetelmä ja siinä käytettävä laite |
| KR101385384B1 (ko) | 2005-06-03 | 2014-04-14 | 보드 오브 리전츠 오브 더 유니버시티 오브 텍사스 시스템 | 단일 패러데이 전극에서의 전기발생 화학발광 및 전기화학 |
| WO2007024790A2 (en) * | 2005-08-22 | 2007-03-01 | Envirofuels, Llc | Flow enhancement compositions for liquid and gases in tubes and pipes |
| US8580194B2 (en) * | 2006-11-23 | 2013-11-12 | Asmag-Holding Gmbh | Device for optoelectronically characterizing samples |
| US20080263939A1 (en) * | 2006-12-08 | 2008-10-30 | Baxter C Edward | Lubricity improver compositions and methods for improving lubricity of hydrocarbon fuels |
| AU2009234422B2 (en) | 2008-04-11 | 2014-05-08 | Board Of Regents Of The University Of Texas System | Method and apparatus for nanoparticle electrogenerated chemiluminescence amplification |
| EP2626693A3 (en) | 2008-05-08 | 2014-10-01 | Board of Regents of the University of Texas System | Luminescent nanostructured materials for use in electrogenerated chemiluminescence |
| US20100261292A1 (en) | 2009-04-10 | 2010-10-14 | Meso Scale Technologies, Llc | Methods for Conducting Assays |
| CA2769380C (en) | 2009-07-27 | 2020-08-25 | Meso Scale Technologies, Llc | Assay apparatuses, consumables and methods |
| CN101957322B (zh) * | 2010-08-17 | 2012-01-11 | 中国科学院苏州纳米技术与纳米仿生研究所 | 一种用于电化学发光检测的流通池及其系统 |
| JP5943927B2 (ja) | 2010-10-14 | 2016-07-05 | メソ スケール テクノロジーズ エルエルシー | アッセイデバイスにおける試薬貯蔵 |
| HUE029030T2 (en) | 2010-10-25 | 2017-01-30 | Hoffmann La Roche | Use of signal repair compounds for electrochemiluminescence detection |
| DK3467500T3 (da) | 2011-11-11 | 2021-08-16 | Eli N Glezer | Cobinder-understøttede assayfremgangsmåder |
| WO2014107576A2 (en) | 2013-01-04 | 2014-07-10 | Meso Scale Technologies, Llc | Assay apparatuses, methods and reagents |
| KR102275006B1 (ko) | 2013-03-11 | 2021-07-09 | 메소 스케일 테크놀러지즈, 엘엘시 | 다중화 어세이를 수행하기 위한 개선된 방법 |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| US9618510B2 (en) | 2013-03-13 | 2017-04-11 | Meso Scale Technologies, Llc. | Assay methods |
| CN105209532B (zh) | 2013-03-15 | 2017-11-28 | 路博润先进材料公司 | 不含重金属的cpvc组合物 |
| US10955412B2 (en) | 2014-05-09 | 2021-03-23 | Meso Scale Technologies, Llc. | Graphene-modified electrodes |
| AU2015259048B2 (en) | 2014-05-15 | 2021-09-09 | Meso Scale Technologies, Llc. | Improved assay methods |
| WO2016091755A1 (en) | 2014-12-08 | 2016-06-16 | F. Hoffmann-La Roche Ag | Method for measurement of vitamin d |
| WO2017153574A1 (en) | 2016-03-11 | 2017-09-14 | Roche Diagnostics Gmbh | Branched-chain amines in electrochemiluminescence detection |
| US10241111B2 (en) | 2016-10-21 | 2019-03-26 | AhuraTech LLC | Electroluminescent binding assays |
| US10159136B2 (en) | 2016-10-21 | 2018-12-18 | AhuraTech LLC | System and method for producing light in a liquid media |
| FI3756009T3 (fi) | 2018-02-23 | 2023-12-01 | Meso Scale Technologies Llc | Menetelmiä antigeeniä sitovien molekyylien seulontaan normalisoimalla antigeeniä sitovan molekyylin pitoisuus |
| CN111351938A (zh) * | 2018-12-20 | 2020-06-30 | 麦德龙生物株式会社 | 电激发标记分子的方法和绝缘膜涂覆的电极 |
| AU2019418498A1 (en) | 2019-01-03 | 2021-07-15 | Meso Scale Technologies, Llc | Compositions and methods for carrying out assay measurements |
| EP3931201A1 (en) | 2019-03-01 | 2022-01-05 | Meso Scale Technologies, LLC | Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same |
| CN113939599B (zh) | 2019-06-07 | 2025-06-06 | 豪夫迈·罗氏有限公司 | 杂交全lna寡核苷酸 |
| US11896979B2 (en) | 2019-07-16 | 2024-02-13 | Meso Scale Technologies, Llc. | Assay apparatuses, methods, and reagents |
| US20240018571A1 (en) | 2020-05-19 | 2024-01-18 | Meso Scale Technologies, Llc. | Methods, compositions, and kits for nucleic acid detection |
| CN116113830A (zh) | 2020-07-01 | 2023-05-12 | 中尺度技术有限责任公司 | 用于测定测量的组合物和方法 |
| JP2023554517A (ja) | 2020-12-22 | 2023-12-27 | エフ． ホフマン－ラ ロシュ アーゲー | 試料中の目的の分析物を検出するための方法 |
| AU2022304570A1 (en) | 2021-06-28 | 2024-01-18 | Meso Scale Technologies, Llc. | Methods, compositions, and kits for assay signal amplification |
| KR20240146115A (ko) | 2021-12-30 | 2024-10-07 | 메소 스케일 테크놀러지즈, 엘엘시 | 전기화학발광 검출 방법 |
| CN118696234A (zh) | 2022-02-18 | 2024-09-24 | 豪夫迈·罗氏有限公司 | 用于检测样品中的目标分析物的方法 |
| EP4505183A1 (en) | 2022-04-07 | 2025-02-12 | Meso Scale Technologies, LLC | Methods and kits for assessing alzheimer's disease |
| US20240229110A1 (en) | 2022-12-20 | 2024-07-11 | Meso Scale Technologies, Llc. | Assay methods and kits |
| WO2025260043A1 (en) | 2024-06-14 | 2025-12-18 | Meso Scale Technologies, Llc. | Methods and kits for measuring three or more tau epitopes |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5238808A (en) * | 1984-10-31 | 1993-08-24 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US5147806A (en) * | 1988-04-29 | 1992-09-15 | Igen, Inc. | Method and apparatus for conducting electrochemiluminescence measurements |
| SE461117B (sv) * | 1988-03-21 | 1990-01-08 | Jouko J Kankare | Foerfarande foer bestaemning av koncentrationen av en luminiscerande lantanid foerening |
| US5308754A (en) * | 1988-03-21 | 1994-05-03 | Kankare Jouko J | Electrogenerated luminescence in solution |
| US5705402A (en) * | 1988-11-03 | 1998-01-06 | Igen International, Inc. | Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets |
| US5746974A (en) * | 1988-11-03 | 1998-05-05 | Igen International, Inc. | Apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence and chemiluminescence detection |
| KR0143993B1 (ko) * | 1988-11-03 | 1998-07-15 | 리차아드 제이 매씨이 | 전기화학 발광성 분석 |
| US5779976A (en) * | 1988-11-03 | 1998-07-14 | Igen International, Inc. | Apparatus for improved luminescence assays |
| DE68928492T2 (de) * | 1988-11-03 | 1998-08-20 | Igen Inc | Elektrochemilumineszente reaktion unter verwendung eines von einem amin abgeleiteten reduktionsmittels |
| EP0476545B1 (en) * | 1990-09-14 | 1997-05-07 | Tosoh Corporation | Method of and kit for immunoassay |
| IL100866A (en) * | 1991-02-06 | 1995-10-31 | Igen Inc | Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets |
| IL100867A (en) * | 1991-02-06 | 1995-12-08 | Igen Inc | Method and apparatus for improved luminescence assays |
| JP3149255B2 (ja) * | 1991-02-19 | 2001-03-26 | ティーディーケイ株式会社 | 電気化学発光物質の分析方法及びそのための装置 |
| US5384265A (en) * | 1993-03-26 | 1995-01-24 | Geo-Centers, Inc. | Biomolecules bound to catalytic inorganic particles, immunoassays using the same |
| US5637508A (en) * | 1993-03-26 | 1997-06-10 | Geo-Centers, Inc. | Biomolecules bound to polymer or copolymer coated catalytic inorganic particles, immunoassays using the same and kits containing the same |
| DE4342942A1 (de) * | 1993-12-16 | 1995-06-22 | Boehringer Mannheim Gmbh | Vorrichtung und Verfahren zur Erzeugung optisch detektierbarer Signale durch Anlegen elektrischer Potentiale an Probeflüssigkeiten |
| US5744367A (en) * | 1994-11-10 | 1998-04-28 | Igen International, Inc. | Magnetic particle based electrochemiluminescent detection apparatus and method |
| US5527710A (en) * | 1994-12-02 | 1996-06-18 | Igen, Inc. | Rate measurements of biomolecular reactions using electrochemiluminescence |
| JPH08190801A (ja) * | 1995-01-12 | 1996-07-23 | Hitachi Ltd | 電気化学発光デバイス |
| US6207369B1 (en) * | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
| CZ299135B6 (cs) * | 1995-03-10 | 2008-04-30 | Meso Scale Technologies, Llc. Corporation Servicecompany | Kazeta a zarízení pro použití pri detekci analytu, zpusob provádení testu za použití uvedené kazety, kit pro použití pri provádení množiny elektrochemiluminescencních testu a zpusob detekce nebo merení analytu |
| AU6476696A (en) * | 1995-06-07 | 1996-12-30 | Igen, Inc. | Simultaneous assay method using lanthanide chelates as the l uminophore for multiple labels |
| US5744376A (en) * | 1996-04-08 | 1998-04-28 | Chartered Semiconductor Manufacturing Pte, Ltd | Method of manufacturing copper interconnect with top barrier layer |
| FI970593A7 (fi) * | 1997-02-12 | 1998-08-13 | Ala Kleme Timo | Pinnoitettujen johteiden käyttö analyyttisiin tarkoituksiin |
| EP0962773A1 (en) * | 1998-06-03 | 1999-12-08 | Mark Howard Jones | Electrochemical based assay processes instrument and labels |
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| WO2001013095A8 (en) | 2001-06-21 |
| CA2380655A1 (en) | 2001-02-22 |
| DE60038803D1 (de) | 2008-06-19 |
| DK1204858T3 (da) | 2008-08-11 |
| EP1204858B1 (en) | 2008-05-07 |
| ATE394663T1 (de) | 2008-05-15 |
| PT1204858E (pt) | 2008-08-07 |
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