ES2281354T3 - Deteccion de diferencias en acidos nucleicos mediante la inhibicion de la migracion espontanea de la ramificacion de adn. - Google Patents
Deteccion de diferencias en acidos nucleicos mediante la inhibicion de la migracion espontanea de la ramificacion de adn. Download PDFInfo
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- ES2281354T3 ES2281354T3 ES00955585T ES00955585T ES2281354T3 ES 2281354 T3 ES2281354 T3 ES 2281354T3 ES 00955585 T ES00955585 T ES 00955585T ES 00955585 T ES00955585 T ES 00955585T ES 2281354 T3 ES2281354 T3 ES 2281354T3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
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- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
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- Zoology (AREA)
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- Health & Medical Sciences (AREA)
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- Molecular Biology (AREA)
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- Physics & Mathematics (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/376,097 US6232104B1 (en) | 1999-08-17 | 1999-08-17 | Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration |
| US376097 | 1999-08-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2281354T3 true ES2281354T3 (es) | 2007-10-01 |
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| ES00955585T Expired - Lifetime ES2281354T3 (es) | 1999-08-17 | 2000-08-15 | Deteccion de diferencias en acidos nucleicos mediante la inhibicion de la migracion espontanea de la ramificacion de adn. |
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| Country | Link |
|---|---|
| US (1) | US6232104B1 (enExample) |
| EP (1) | EP1210457B1 (enExample) |
| JP (1) | JP2003507025A (enExample) |
| DE (1) | DE60033825T2 (enExample) |
| ES (1) | ES2281354T3 (enExample) |
| WO (1) | WO2001012859A2 (enExample) |
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| ES2370232T3 (es) * | 2000-05-04 | 2011-12-13 | Siemens Healthcare Diagnostics Products Gmbh | Procedimiento para la detección de múltiples analitos. |
| JP4982921B2 (ja) * | 2001-03-05 | 2012-07-25 | ルネサスエレクトロニクス株式会社 | 半導体装置及びその製造方法 |
| US6878530B2 (en) * | 2002-02-07 | 2005-04-12 | Panomics, Inc. | Methods for detecting polymorphisms in nucleic acids |
| GB0310270D0 (en) * | 2003-05-03 | 2003-06-11 | Univ Edinburgh | Biomolecular devices |
| US7273762B2 (en) * | 2004-11-09 | 2007-09-25 | Freescale Semiconductor, Inc. | Microelectromechanical (MEM) device including a spring release bridge and method of making the same |
| GB0518867D0 (en) * | 2005-09-15 | 2005-10-26 | Secretary Trade Ind Brit | Microscopy tip |
| US20140302486A1 (en) * | 2011-09-02 | 2014-10-09 | President And Fellows Of Harvard College | Systems and methods for detecting biomarkers of interest |
| SG10201510189WA (en) | 2011-10-19 | 2016-01-28 | Nugen Technologies Inc | Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing |
| CN104093890B (zh) | 2012-01-26 | 2016-04-20 | 纽亘技术公司 | 用于靶向核酸序列富集和高效文库产生的组合物和方法 |
| ES2905448T3 (es) | 2012-05-10 | 2022-04-08 | Massachusetts Gen Hospital | Métodos para determinar una secuencia nucleotídica |
| US9957549B2 (en) | 2012-06-18 | 2018-05-01 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| EP2738263A1 (en) * | 2012-11-30 | 2014-06-04 | Fundacion para la Investigacion del Hospital Clinico de la Comunidad Valenciana | Methods for DNA rearrengements analysis |
| EP2971130A4 (en) | 2013-03-15 | 2016-10-05 | Nugen Technologies Inc | SEQUENTIAL SEQUENCING |
| CN105849264B (zh) | 2013-11-13 | 2019-09-27 | 纽亘技术公司 | 用于鉴别重复测序读数的组合物和方法 |
| WO2015112974A1 (en) | 2014-01-27 | 2015-07-30 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| EP3287528A1 (de) | 2016-08-25 | 2018-02-28 | AGCT GmbH | Verfahren zur amplifikation von nukleinsäuren und kit zu dessen durchführung |
| JP6997772B2 (ja) | 2016-09-15 | 2022-01-18 | アーチャーディーエックス， エルエルシー | 核酸サンプル調製の方法 |
| EP4353830A3 (en) | 2016-09-15 | 2024-07-03 | ArcherDX, LLC | Methods of nucleic acid sample preparation for analysis of cell-free dna |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| AU2020218190A1 (en) * | 2019-02-04 | 2021-08-26 | Akoya Biosciences, Inc. | Analyte detection by selective labeling of biological samples |
| US12059674B2 (en) | 2020-02-03 | 2024-08-13 | Tecan Genomics, Inc. | Reagent storage system |
Family Cites Families (43)
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| CA1254114A (en) | 1984-05-07 | 1989-05-16 | Allied Corporation | Displacement polynucleotide assay employing recombination protein and diagnostic kit |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| JP2650159B2 (ja) | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
| US5622820A (en) | 1988-03-10 | 1997-04-22 | City Of Hope | Method for amplification and detection of RNA and DNA sequences |
| EP0433363A4 (en) | 1988-09-08 | 1992-05-06 | The Salk Institute For Biological Studies | Replicative rna-based amplification detection systems |
| US5508178A (en) | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
| US5556750A (en) | 1989-05-12 | 1996-09-17 | Duke University | Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems |
| US5035996A (en) | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| US5766849A (en) | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
| GB8920097D0 (en) | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
| US5187066A (en) | 1990-02-14 | 1993-02-16 | Syntex (U.S.A.) Inc. | Methods for detecting amphiphilic antigens |
| CA2037349C (en) | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
| HU218095B (hu) | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
| US5595891A (en) | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
| US5525462A (en) | 1991-05-02 | 1996-06-11 | Toyo Boseki Kabushiki Kaisha | Nucleic acid sequence amplification method, detection method, and reagent kit therefor |
| DE69213112T2 (de) * | 1991-06-20 | 1997-04-03 | Hoffmann La Roche | Verbesserte Methoden zur Nukleinsäure-Amplifizierung |
| US5582981A (en) | 1991-08-14 | 1996-12-10 | Gilead Sciences, Inc. | Method for identifying an oligonucleotide aptamer specific for a target |
| EP0543484B1 (en) | 1991-08-30 | 2001-01-31 | Research Development Corporation of Japan | A method of DNA amplification |
| DE4213029A1 (de) | 1991-09-26 | 1993-04-01 | Boehringer Mannheim Gmbh | Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen |
| CA2100159C (en) | 1991-11-15 | 2003-01-21 | John R. Link | Rapid assays for amplification products |
| US5567583A (en) | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
| WO1993017127A1 (en) | 1992-02-20 | 1993-09-02 | The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University | Boomerand dna amplification |
| FR2690691B1 (fr) | 1992-04-29 | 1999-02-12 | Bio Merieux | Procede d'amplification d'arn necessitant une seule etape de manipulation. |
| CA2135073C (en) | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| DK0653066T3 (da) | 1992-07-31 | 1998-09-23 | Dade Behring Marburg Gmbh | Fotoaktiverbare kemiluminescerende matricer |
| US5340728A (en) | 1992-12-09 | 1994-08-23 | E. I. Du Pont De Nemours And Company | Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction |
| US5593840A (en) | 1993-01-27 | 1997-01-14 | Oncor, Inc. | Amplification of nucleic acid sequences |
| US5616464A (en) | 1994-12-27 | 1997-04-01 | Naxcor | Nucleic acid sequence detection employing amplification probes |
| US5470723A (en) | 1993-05-05 | 1995-11-28 | Becton, Dickinson And Company | Detection of mycobacteria by multiplex nucleic acid amplification |
| US5422252A (en) * | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
| KR100189229B1 (ko) * | 1993-07-23 | 1999-06-01 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | 핵산 증폭을 강화하는 방법 |
| AU7551594A (en) | 1993-07-29 | 1995-02-28 | MURASHIGE, Kate H. | Method for recognition of the nucleotide sequence of a purified dna segment |
| US5556773A (en) | 1993-08-06 | 1996-09-17 | Yourno; Joseph | Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes |
| US5480783A (en) | 1994-03-31 | 1996-01-02 | The Perkin-Elmer Corporation | Method for reducing background signals in DNA replication/detection assays |
| US5580730A (en) | 1994-08-19 | 1996-12-03 | Olympus America, Inc. | Enzyme digestion method for the detection of amplified DNA |
| FR2724934B1 (fr) | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
| US5512441A (en) | 1994-11-15 | 1996-04-30 | American Health Foundation | Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method |
| US5710029A (en) | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| FR2737223B1 (fr) * | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
| US5681705A (en) | 1995-08-28 | 1997-10-28 | Schram; James L. | Amplification and detection of mycobacterium avium complex species |
| US5780231A (en) | 1995-11-17 | 1998-07-14 | Lynx Therapeutics, Inc. | DNA extension and analysis with rolling primers |
| US6013439A (en) * | 1995-12-22 | 2000-01-11 | Dade Behring Marburg Gmbh | Detection of differences in nucleic acids |
| WO2000043546A2 (en) * | 1999-01-19 | 2000-07-27 | Dade Behring Inc. | Detection of drug resistant organisms |
-
1999
- 1999-08-17 US US09/376,097 patent/US6232104B1/en not_active Expired - Fee Related
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2000
- 2000-08-15 EP EP00955585A patent/EP1210457B1/en not_active Expired - Lifetime
- 2000-08-15 WO PCT/US2000/022446 patent/WO2001012859A2/en not_active Ceased
- 2000-08-15 JP JP2001516946A patent/JP2003507025A/ja active Pending
- 2000-08-15 DE DE60033825T patent/DE60033825T2/de not_active Expired - Lifetime
- 2000-08-15 ES ES00955585T patent/ES2281354T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001012859A2 (en) | 2001-02-22 |
| US6232104B1 (en) | 2001-05-15 |
| EP1210457A2 (en) | 2002-06-05 |
| JP2003507025A (ja) | 2003-02-25 |
| DE60033825D1 (de) | 2007-04-19 |
| WO2001012859A3 (en) | 2002-01-31 |
| DE60033825T2 (de) | 2007-11-08 |
| EP1210457B1 (en) | 2007-03-07 |
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