ES2237562T3 - Procedimiento y kit para detectar la esquistosomiasis mediante la reaccion en cadena de la polimerasa. - Google Patents
Procedimiento y kit para detectar la esquistosomiasis mediante la reaccion en cadena de la polimerasa.Info
- Publication number
- ES2237562T3 ES2237562T3 ES01916775T ES01916775T ES2237562T3 ES 2237562 T3 ES2237562 T3 ES 2237562T3 ES 01916775 T ES01916775 T ES 01916775T ES 01916775 T ES01916775 T ES 01916775T ES 2237562 T3 ES2237562 T3 ES 2237562T3
- Authority
- ES
- Spain
- Prior art keywords
- dna
- pcr
- seq
- schistosoma
- mansoni
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 51
- 238000006243 chemical reaction Methods 0.000 title description 15
- 241000242678 Schistosoma Species 0.000 claims abstract description 38
- 230000003321 amplification Effects 0.000 claims abstract description 27
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 27
- 238000001514 detection method Methods 0.000 claims abstract description 25
- 238000003745 diagnosis Methods 0.000 claims abstract description 11
- 239000012472 biological sample Substances 0.000 claims abstract description 8
- 238000001962 electrophoresis Methods 0.000 claims abstract description 7
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 241000242680 Schistosoma mansoni Species 0.000 claims description 44
- 239000013615 primer Substances 0.000 claims description 34
- 244000045947 parasite Species 0.000 claims description 19
- 238000007400 DNA extraction Methods 0.000 claims description 5
- 238000012408 PCR amplification Methods 0.000 claims description 5
- 241000242679 Schistosoma bovis Species 0.000 claims description 5
- 241000242683 Schistosoma haematobium Species 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 238000010186 staining Methods 0.000 claims description 5
- 239000003155 DNA primer Substances 0.000 claims description 4
- 108091034117 Oligonucleotide Proteins 0.000 claims description 4
- 229920002401 polyacrylamide Polymers 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 7
- 201000004409 schistosomiasis Diseases 0.000 abstract description 6
- 108020004414 DNA Proteins 0.000 description 61
- 238000003752 polymerase chain reaction Methods 0.000 description 49
- 239000002987 primer (paints) Substances 0.000 description 32
- 235000013601 eggs Nutrition 0.000 description 22
- 208000015181 infectious disease Diseases 0.000 description 17
- 210000003608 fece Anatomy 0.000 description 14
- 239000000523 sample Substances 0.000 description 9
- 230000000295 complement effect Effects 0.000 description 8
- 230000035945 sensitivity Effects 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 239000003550 marker Substances 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 241000242677 Schistosoma japonicum Species 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 150000007523 nucleic acids Chemical class 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 108010006785 Taq Polymerase Proteins 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 230000003252 repetitive effect Effects 0.000 description 4
- 241000224467 Giardia intestinalis Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 230000003108 parasitologic effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 108020004463 18S ribosomal RNA Proteins 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 108091060211 Expressed sequence tag Proteins 0.000 description 2
- 206010061217 Infestation Diseases 0.000 description 2
- 241000237852 Mollusca Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-N dCTP Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO[P@](O)(=O)O[P@](O)(=O)OP(O)(O)=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-N 0.000 description 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 244000000013 helminth Species 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 238000012800 visualization Methods 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 206010001935 American trypanosomiasis Diseases 0.000 description 1
- 241000244185 Ascaris lumbricoides Species 0.000 description 1
- 241000295636 Cryptosporidium sp. Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108020001019 DNA Primers Proteins 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 238000009007 Diagnostic Kit Methods 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000237858 Gastropoda Species 0.000 description 1
- 102000000521 Immunophilins Human genes 0.000 description 1
- 108010016648 Immunophilins Proteins 0.000 description 1
- 241001137872 Leishmania sp. Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 240000009188 Phyllostachys vivax Species 0.000 description 1
- 241000223960 Plasmodium falciparum Species 0.000 description 1
- 241000223821 Plasmodium malariae Species 0.000 description 1
- 206010035501 Plasmodium malariae infection Diseases 0.000 description 1
- 241000244157 Taenia solium Species 0.000 description 1
- 241000869417 Trematodes Species 0.000 description 1
- 241000223109 Trypanosoma cruzi Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 235000019506 cigar Nutrition 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 210000004258 portal system Anatomy 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 235000008001 rakum palm Nutrition 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR0001536 | 2000-04-04 | ||
| BRPI0001536A BRPI0001536B1 (pt) | 2000-04-04 | 2000-04-04 | método para diagnosticar a infecção por parasitos do genero schistosoma e kit para uso no diagnóstico da infecção por schistosoma sp |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2237562T3 true ES2237562T3 (es) | 2005-08-01 |
Family
ID=77336322
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES01916775T Expired - Lifetime ES2237562T3 (es) | 2000-04-04 | 2001-04-04 | Procedimiento y kit para detectar la esquistosomiasis mediante la reaccion en cadena de la polimerasa. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6818402B2 (enExample) |
| EP (1) | EP1208241B1 (enExample) |
| JP (1) | JP4974432B2 (enExample) |
| CN (1) | CN1366553A (enExample) |
| AT (1) | ATE286984T1 (enExample) |
| AU (1) | AU4397101A (enExample) |
| BR (1) | BRPI0001536B1 (enExample) |
| DE (1) | DE60108337T2 (enExample) |
| ES (1) | ES2237562T3 (enExample) |
| WO (1) | WO2001075148A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7244125B2 (en) | 2003-12-08 | 2007-07-17 | Neoconix, Inc. | Connector for making electrical contact at semiconductor scales |
| US7056131B1 (en) | 2003-04-11 | 2006-06-06 | Neoconix, Inc. | Contact grid array system |
| US7113408B2 (en) | 2003-06-11 | 2006-09-26 | Neoconix, Inc. | Contact grid array formed on a printed circuit board |
| US7090503B2 (en) | 2004-03-19 | 2006-08-15 | Neoconix, Inc. | Interposer with compliant pins |
| US7347698B2 (en) | 2004-03-19 | 2008-03-25 | Neoconix, Inc. | Deep drawn electrical contacts and method for making |
| US7025601B2 (en) | 2004-03-19 | 2006-04-11 | Neoconix, Inc. | Interposer and method for making same |
| TWI309094B (en) | 2004-03-19 | 2009-04-21 | Neoconix Inc | Electrical connector in a flexible host and method for fabricating the same |
| CN100383162C (zh) * | 2005-05-10 | 2008-04-23 | 安徽医科大学 | 用于血吸虫病检测的组合检测试剂及检测方法 |
| AU2007233252C1 (en) | 2006-03-29 | 2013-05-23 | Boehringer Ingelheim Animal Health USA Inc. | Vaccine against Streptococci |
| CN101457258B (zh) * | 2008-12-26 | 2011-06-15 | 江苏省血吸虫病防治研究所 | 一种血吸虫感染性钉螺检测试剂盒及其检测方法 |
| US20120164238A1 (en) | 2009-02-03 | 2012-06-28 | Mdxhealth Sa | Methods of detecting colorectal cancer |
| CN101597645B (zh) * | 2009-06-26 | 2012-05-30 | 华南农业大学 | 一种日本血吸虫性别特异性检测引物、检测试剂盒和检测方法 |
| WO2011006449A1 (zh) * | 2009-07-16 | 2011-01-20 | 上海人类基因组研究中心 | 日本血吸虫的反转座子及其用途 |
| RU2509156C1 (ru) * | 2012-06-19 | 2014-03-10 | Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук | Способ видовой днк-дифференциации на разных стадиях жизненного цикла гельминтов-возбудителей церкариального дерматита человека |
| CN107532124B (zh) | 2015-03-27 | 2022-08-09 | 精密科学公司 | 检测食管疾病 |
| CN110760588A (zh) * | 2019-07-18 | 2020-02-07 | 中国农业科学院上海兽医研究所(中国动物卫生与流行病学中心上海分中心) | 一种用于日本血吸虫检测的核酸诊断试剂盒及其检测方法 |
| CN110791576A (zh) * | 2019-12-23 | 2020-02-14 | 江苏省血吸虫病防治研究所 | 检测曼氏血吸虫的引物组、探针及试剂盒 |
| CN113151496A (zh) * | 2021-04-20 | 2021-07-23 | 中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心) | Lfd-rpa可视化快速检测曼氏血吸虫核酸的引物、探针、试剂盒及方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997005158A1 (en) * | 1994-07-25 | 1997-02-13 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions, treatments, and diagnostics for schistosomiasis and related diseases |
-
2000
- 2000-04-04 BR BRPI0001536A patent/BRPI0001536B1/pt active IP Right Grant
-
2001
- 2001-04-04 DE DE60108337T patent/DE60108337T2/de not_active Expired - Fee Related
- 2001-04-04 ES ES01916775T patent/ES2237562T3/es not_active Expired - Lifetime
- 2001-04-04 CN CN01800817A patent/CN1366553A/zh active Pending
- 2001-04-04 JP JP2001573022A patent/JP4974432B2/ja not_active Expired - Fee Related
- 2001-04-04 WO PCT/BR2001/000035 patent/WO2001075148A1/en not_active Ceased
- 2001-04-04 US US09/980,277 patent/US6818402B2/en not_active Expired - Lifetime
- 2001-04-04 AU AU43971/01A patent/AU4397101A/en not_active Abandoned
- 2001-04-04 AT AT01916775T patent/ATE286984T1/de not_active IP Right Cessation
- 2001-04-04 EP EP01916775A patent/EP1208241B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2003529373A (ja) | 2003-10-07 |
| US6818402B2 (en) | 2004-11-16 |
| US20030096244A1 (en) | 2003-05-22 |
| DE60108337D1 (de) | 2005-02-17 |
| EP1208241B1 (en) | 2005-01-12 |
| BRPI0001536B8 (enExample) | 2021-07-06 |
| JP4974432B2 (ja) | 2012-07-11 |
| BRPI0001536B1 (pt) | 2015-09-29 |
| AU4397101A (en) | 2001-10-15 |
| EP1208241A1 (en) | 2002-05-29 |
| CN1366553A (zh) | 2002-08-28 |
| BRPI0001536A (pt) | 2001-12-04 |
| WO2001075148A1 (en) | 2001-10-11 |
| DE60108337T2 (de) | 2005-12-22 |
| ATE286984T1 (de) | 2005-01-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2237562T3 (es) | Procedimiento y kit para detectar la esquistosomiasis mediante la reaccion en cadena de la polimerasa. | |
| Pontes et al. | Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces. | |
| Giacani et al. | The endemic treponematoses | |
| Kwakye-Nuako et al. | First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex | |
| NL193663C (nl) | Werkwijze en kit voor het detecteren van een specifiek nucleïnezuur in een monster. | |
| ES2397115T3 (es) | Cebadores para detectar Plasmodium | |
| ES2471092T3 (es) | Sondas de ácido nucleico y métodos para detectar parásitos de Plasmodium | |
| Kelly et al. | Rickettsia felis, Bartonella henselae, and B. clarridgeiae, New Zealand | |
| Palenzuela et al. | Development of a polymerase chain reaction diagnostic assay for Ceratomyxa shasta, a myxosporean parasite of salmonid fish | |
| Huang et al. | In situ hybridization of a marine fish virus, Singapore grouper iridovirus with a nucleic acid probe of major capsid protein | |
| Brindley et al. | Characterization of a programmed alteration in an 18S ribosomal gene that accompanies the experimental induction of drug resistance in Schistosoma mansoni. | |
| Abollo et al. | Fitness of the marine parasitic nematode Anisakis simplex s. str. in temperate waters of the NE Atlantic | |
| Nolan et al. | Two new blood flukes (Digenea: Sanguinicolidae) from Epinephelinae (Perciformes: Serranidae) of the Pacific Ocean | |
| Rognlie et al. | Myxobolus cerebralis in Tubifex tubifex from a whirling disease epizootic in Montana | |
| JP2000511058A (ja) | Ｍｙｃｏｂａｃｔｅｒｉｕｍ Ｋａｎｓａｓｉｉを検出するための組成物および方法 | |
| Fazaeli et al. | Clinical features of cutaneous leishmaniasis and direct pcrbased identification of parasite species in a new focus in Southeast of Iran | |
| Abel et al. | Hammondia isolated from dogs and foxes are genetically distinct | |
| Du Preez et al. | A new genus of polystomatid parasitic flatworm (Monogenea: Polystomatidae) without free-swimming life stage from the Malagasy poison frogs | |
| CN109762910A (zh) | 一种用于同时检测两型包虫病的引物及试剂盒 | |
| Singh et al. | Amebas | |
| Tiwari et al. | Characterization of an Indian bluetongue virus isolate by RT-PCR and restriction enzyme analysis of the VP-7 gene sequence | |
| RU2332465C1 (ru) | Способ идентификации возбудителя цистного эхинококкоза в биологическом образце | |
| EP3916111A1 (en) | General-purpose primer sets for detecting flaviviruses and use thereof | |
| JP2004514437A (ja) | ネグレリア属の原虫を検出する方法 | |
| RU2824209C1 (ru) | Набор олигонуклеотидных праймеров для выявления РНК вируса желтой лихорадки методом изотермической амплификации в режиме реального времени |