ES2200728T1 - Clonacion y expresion de una lipasa extracelular acidorresistente de yarrowia lipolytica. - Google Patents
Clonacion y expresion de una lipasa extracelular acidorresistente de yarrowia lipolytica.Info
- Publication number
- ES2200728T1 ES2200728T1 ES00925351T ES00925351T ES2200728T1 ES 2200728 T1 ES2200728 T1 ES 2200728T1 ES 00925351 T ES00925351 T ES 00925351T ES 00925351 T ES00925351 T ES 00925351T ES 2200728 T1 ES2200728 T1 ES 2200728T1
- Authority
- ES
- Spain
- Prior art keywords
- lipase
- nucleic acid
- acid fragment
- fragment according
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 101710158368 Extracellular lipase Proteins 0.000 title claims abstract 5
- 101710128940 Triacylglycerol lipase Proteins 0.000 title claims abstract 5
- 241000235015 Yarrowia lipolytica Species 0.000 title claims abstract 4
- 238000010367 cloning Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 8
- 108090001060 Lipase Proteins 0.000 claims abstract 6
- 102000004882 Lipase Human genes 0.000 claims abstract 6
- 239000004367 Lipase Substances 0.000 claims abstract 6
- 235000019421 lipase Nutrition 0.000 claims abstract 6
- 239000002253 acid Substances 0.000 claims abstract 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract 3
- 238000004519 manufacturing process Methods 0.000 claims abstract 2
- 102000039446 nucleic acids Human genes 0.000 claims abstract 2
- 108020004707 nucleic acids Proteins 0.000 claims abstract 2
- 229920001184 polypeptide Polymers 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 210000004027 cell Anatomy 0.000 claims 3
- 230000003834 intracellular effect Effects 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 239000002243 precursor Substances 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 claims 1
- 210000001236 prokaryotic cell Anatomy 0.000 claims 1
- 230000028327 secretion Effects 0.000 claims 1
- 241001235480 Nakazawaea ernobii Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fertilizers (AREA)
Abstract
Módulo de expresión de una lipasa extracelular acidorresistente de levadura, caracterizado porque comprende: - un fragmento de ácido nucleico según cualquiera de las reivindicaciones 1 a 4; - secuencias que permiten regular la expresión de dicha lipasa en una célula huésped.
Claims (10)
1. Fragmento de ácido nucleico, que comprende una
secuencia que codifica una lipasa extracelular acidorresistente de
levadura, caracterizado porque el precursor intracelular de
dicha lipasa presenta en la secuencia polipeptídica, al menos el
30% de identidad, y al menos el 39% de similaridad con el precursor
intracelular de la lipasa extracelular de Y. Lipolytica.
2. Fragmento de ácido nucleico según la
reivindicación 1, caracterizado porque comprende al menos
una secuencia nucleotídica que codifica un polipéptido que
comprende los aminoácidos +1 a +301 del polipéptido ID SEQ nº:
2.
3. Fragmento de ácido nucleico según cualquiera
de las reivindicaciones 1 ó 2, caracterizado porque
comprende, además, una secuencia que codifica un polipéptido que
permite la secreción de dicha lipasa.
4. Fragmento de ácido nucleico según la
reivindicación 3, caracterizado porque comprende la
secuencia de nucleótidos 3737 a 3835 de la secuencia ID SEQ nº:
1.
5. Módulo de expresión de una lipasa extracelular
acidorresistente de levadura, caracterizado porque
comprende:
- un fragmento de ácido nucleico según cualquiera
de las reivindicaciones 1 a 4;
- secuencias que permiten regular la expresión de
dicha lipasa en una célula huésped.
6. Vector recombinante que comprende al menos un
fragmento de ácido nucleico según cualquiera de las
reivindicaciones 1 a 4.
7. Vector recombinante según la reivindicación 6,
que comprende al menos un módulo de expresión según la
reivindicación 5.
8. Célula eucariota o procariota transformada por
al menos un fragmento de ácido nucleico según cualquiera de las
reivindicaciones 1 a 4.
9. Cepa de células transformada según la
reivindicación 8, caracterizada porque se trata del clón de
Yarrowia lipolytica denominada MS4, registrada en la CNCM
con el número I-2294.
10. Procedimiento de producción de una lipasa
acidorresistente, caracterizado porque comprende al menos
una etapa durante la cual se procede al cultivo de células
transformadas según cualquiera de las reivindicaciones 8 ó 9, y una
etapa durante la cual se recoge la lipasa recombinante producida
por dichas células.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/FR2000/001148 WO2001083773A1 (fr) | 2000-04-28 | 2000-04-28 | Clonage et expression d'une lipase extracellulaire acidoresistante de yarrowia lipolytica |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2200728T1 true ES2200728T1 (es) | 2004-03-16 |
| ES2200728T3 ES2200728T3 (es) | 2008-04-16 |
Family
ID=8846450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES00925351T Expired - Lifetime ES2200728T3 (es) | 2000-04-28 | 2000-04-28 | Clonacion y expresion de una lipasa extracelular acidorresistente de yarrowia lipolytica. |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1276874B1 (es) |
| AT (2) | ATE374823T1 (es) |
| AU (1) | AU2000244105A1 (es) |
| CY (1) | CY1107119T1 (es) |
| DE (2) | DE60036647T2 (es) |
| DK (1) | DK1276874T3 (es) |
| ES (1) | ES2200728T3 (es) |
| HK (1) | HK1053145B (es) |
| PT (1) | PT1276874E (es) |
| WO (1) | WO2001083773A1 (es) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7238482B2 (en) | 2003-05-07 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
| US7259255B2 (en) | 2003-06-25 | 2007-08-21 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
| US7459546B2 (en) | 2003-06-25 | 2008-12-02 | E.I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast |
| CN101084307B (zh) * | 2004-09-30 | 2011-06-01 | 诺维信股份有限公司 | 具有脂肪酶活性的多肽和编码其的多核苷酸 |
| US7550286B2 (en) | 2004-11-04 | 2009-06-23 | E. I. Du Pont De Nemours And Company | Docosahexaenoic acid producing strains of Yarrowia lipolytica |
| KR100644924B1 (ko) * | 2005-04-15 | 2006-11-10 | 한국해양연구원 | 옥플록사신 에스테르에 대한 광학선택적 리파제, 이를암호화하는 뉴클레오타이드 및 이를 이용하여레보플록사신을 제조하는 방법 |
| CN100424170C (zh) * | 2005-10-11 | 2008-10-08 | 北京化工大学 | 脂肪酶、其基因、产生该酶的亚罗解脂酵母及其应用 |
| WO2007144475A1 (fr) | 2006-06-15 | 2007-12-21 | Laboratoires Mayoly Spindler | Procede de production de lipase, cellule de yarrowia lipolytica transformee apte a produire ladite lipase et leurs applications |
| CN102703404B (zh) * | 2006-06-15 | 2015-06-10 | 法国米奥里大药厂 | 用于生产脂肪酶的方法、能产生所述脂肪酶的转化解脂耶氏酵母细胞以及它们的应用 |
| FR2927089B1 (fr) | 2008-02-05 | 2011-03-25 | Inst Nat De La Rech Agronomique Inra | Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia |
| ES2575008T3 (es) | 2008-07-11 | 2016-06-23 | Nstitut National De La Recherche Agronomique (Inra) | Nuevas cepas de levadura mutantes capaces de acumular una gran cantidad de lípidos |
| FR2962133B1 (fr) | 2010-07-01 | 2014-09-12 | Agronomique Inst Nat Rech | Optimisation de la synthese et de l'accumulation de lipides |
| FR3005317B1 (fr) | 2013-05-02 | 2016-03-18 | Agronomique Inst Nat Rech | Levures mutantes capables de produire un acide gras inhabituel |
| WO2015189352A1 (en) | 2014-06-11 | 2015-12-17 | Institut National De La Recherche Agronomique | Improved lipid accumulation in yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof |
| WO2016075314A1 (fr) | 2014-11-13 | 2016-05-19 | Institut National De La Recherche Agronomique | Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines |
| FR3028527A1 (fr) | 2014-11-13 | 2016-05-20 | Pivert | Identification de facteurs de transcription de yarrowia lipolytica |
| EP3085788A1 (en) | 2015-04-23 | 2016-10-26 | Institut National De La Recherche Agronomique | Mutant yeast strain capable of degrading cellobiose |
| EP3106520A1 (en) | 2015-06-17 | 2016-12-21 | Institut National De La Recherche Agronomique | Mutant yarrowia strain capable of degrading galactose |
| US20190136278A1 (en) | 2016-05-10 | 2019-05-09 | Institut National De La Recherche Agronomique | Mutant yeast strains with enhanced production of erythritol or erythrulose |
| EP3348647A1 (en) | 2017-01-13 | 2018-07-18 | Institut National De La Recherche Agronomique | Mutant yeast strain capable of producing medium chain fatty acids |
| EP3360956A1 (en) | 2017-02-10 | 2018-08-15 | Institut National De La Recherche Agronomique | Mutant yeast strain capable of degrading cellulose |
| AU2022340887A1 (en) * | 2021-09-01 | 2024-03-14 | Chr. Hansen A/S | Yarrowia lipolytica and its use for producing lipases specifically liberating short chain fatty acids |
-
2000
- 2000-04-28 PT PT00925351T patent/PT1276874E/pt unknown
- 2000-04-28 AT AT00925351T patent/ATE374823T1/de active
- 2000-04-28 DE DE60036647T patent/DE60036647T2/de not_active Expired - Lifetime
- 2000-04-28 DE DE0001276874T patent/DE00925351T1/de active Pending
- 2000-04-28 WO PCT/FR2000/001148 patent/WO2001083773A1/fr active IP Right Grant
- 2000-04-28 DK DK00925351T patent/DK1276874T3/da active
- 2000-04-28 EP EP00925351A patent/EP1276874B1/fr not_active Expired - Lifetime
- 2000-04-28 ES ES00925351T patent/ES2200728T3/es not_active Expired - Lifetime
- 2000-04-28 AU AU2000244105A patent/AU2000244105A1/en not_active Abandoned
- 2000-07-24 AT AT00946470T patent/ATE375525T1/de not_active IP Right Cessation
-
2003
- 2003-07-22 HK HK03105294.9A patent/HK1053145B/zh unknown
-
2008
- 2008-01-02 CY CY20081100005T patent/CY1107119T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ATE374823T1 (de) | 2007-10-15 |
| EP1276874A1 (fr) | 2003-01-22 |
| DE00925351T1 (de) | 2004-04-15 |
| ES2200728T3 (es) | 2008-04-16 |
| DE60036647D1 (de) | 2007-11-15 |
| CY1107119T1 (el) | 2012-10-24 |
| AU2000244105A1 (en) | 2001-11-12 |
| ATE375525T1 (de) | 2007-10-15 |
| WO2001083773A1 (fr) | 2001-11-08 |
| HK1053145A1 (en) | 2003-10-10 |
| DE60036647T2 (de) | 2008-07-17 |
| PT1276874E (pt) | 2008-01-11 |
| DK1276874T3 (da) | 2008-02-04 |
| EP1276874B1 (fr) | 2007-10-03 |
| HK1053145B (zh) | 2008-02-15 |
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