DE60036647D1 - Klonierung und expression einer extrazellulären säurebeständigen lipase aus yarrowia lipolytica - Google Patents
Klonierung und expression einer extrazellulären säurebeständigen lipase aus yarrowia lipolyticaInfo
- Publication number
- DE60036647D1 DE60036647D1 DE60036647T DE60036647T DE60036647D1 DE 60036647 D1 DE60036647 D1 DE 60036647D1 DE 60036647 T DE60036647 T DE 60036647T DE 60036647 T DE60036647 T DE 60036647T DE 60036647 D1 DE60036647 D1 DE 60036647D1
- Authority
- DE
- Germany
- Prior art keywords
- cloning
- expression
- yarrowia lipolytica
- resistant lipase
- extracellular acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090001060 Lipase Proteins 0.000 title abstract 2
- 102000004882 Lipase Human genes 0.000 title abstract 2
- 239000004367 Lipase Substances 0.000 title abstract 2
- 241000235015 Yarrowia lipolytica Species 0.000 title abstract 2
- 235000019421 lipase Nutrition 0.000 title abstract 2
- 238000010367 cloning Methods 0.000 title 1
- 101710158368 Extracellular lipase Proteins 0.000 abstract 1
- 241001235480 Nakazawaea ernobii Species 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 101710128940 Triacylglycerol lipase Proteins 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fertilizers (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/FR2000/001148 WO2001083773A1 (fr) | 2000-04-28 | 2000-04-28 | Clonage et expression d'une lipase extracellulaire acidoresistante de yarrowia lipolytica |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60036647D1 true DE60036647D1 (de) | 2007-11-15 |
| DE60036647T2 DE60036647T2 (de) | 2008-07-17 |
Family
ID=8846450
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60036647T Expired - Lifetime DE60036647T2 (de) | 2000-04-28 | 2000-04-28 | Klonierung und expression einer extrazellulären säurebeständigen lipase aus yarrowia lipolytica |
| DE0001276874T Pending DE00925351T1 (de) | 2000-04-28 | 2000-04-28 | Klonierung und expression einer extrazellulären säurebeständigen lipase aus yarrowia lipolytica |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE0001276874T Pending DE00925351T1 (de) | 2000-04-28 | 2000-04-28 | Klonierung und expression einer extrazellulären säurebeständigen lipase aus yarrowia lipolytica |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1276874B1 (de) |
| AT (2) | ATE374823T1 (de) |
| AU (1) | AU2000244105A1 (de) |
| CY (1) | CY1107119T1 (de) |
| DE (2) | DE60036647T2 (de) |
| DK (1) | DK1276874T3 (de) |
| ES (1) | ES2200728T3 (de) |
| HK (1) | HK1053145B (de) |
| PT (1) | PT1276874E (de) |
| WO (1) | WO2001083773A1 (de) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7238482B2 (en) | 2003-05-07 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
| US7459546B2 (en) | 2003-06-25 | 2008-12-02 | E.I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast |
| US7259255B2 (en) | 2003-06-25 | 2007-08-21 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
| WO2006039541A2 (en) | 2004-09-30 | 2006-04-13 | Novozymes, Inc. | Polypeptides having lipase activity and polynucleotides encoding same |
| US7588931B2 (en) | 2004-11-04 | 2009-09-15 | E. I. Du Pont De Nemours And Company | High arachidonic acid producing strains of Yarrowia lipolytica |
| KR100644924B1 (ko) * | 2005-04-15 | 2006-11-10 | 한국해양연구원 | 옥플록사신 에스테르에 대한 광학선택적 리파제, 이를암호화하는 뉴클레오타이드 및 이를 이용하여레보플록사신을 제조하는 방법 |
| CN100424170C (zh) * | 2005-10-11 | 2008-10-08 | 北京化工大学 | 脂肪酶、其基因、产生该酶的亚罗解脂酵母及其应用 |
| CN102703404B (zh) * | 2006-06-15 | 2015-06-10 | 法国米奥里大药厂 | 用于生产脂肪酶的方法、能产生所述脂肪酶的转化解脂耶氏酵母细胞以及它们的应用 |
| JP5592648B2 (ja) * | 2006-06-15 | 2014-09-17 | ラボラトワール マヨリ スパンドレ | リパーゼの製造方法、該リパーゼを産生できる形質転換されたヤロウィアリポリティカ細胞およびその使用 |
| FR2927089B1 (fr) | 2008-02-05 | 2011-03-25 | Inst Nat De La Rech Agronomique Inra | Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia |
| DK2310517T3 (en) | 2008-07-11 | 2016-06-06 | Inst Nat De La Rech Agronomique (Inra) | Novel mutant yeast strains having a capability to accumulate a large amount of lipids |
| FR2962133B1 (fr) | 2010-07-01 | 2014-09-12 | Agronomique Inst Nat Rech | Optimisation de la synthese et de l'accumulation de lipides |
| FR3005317B1 (fr) | 2013-05-02 | 2016-03-18 | Agronomique Inst Nat Rech | Levures mutantes capables de produire un acide gras inhabituel |
| WO2015189352A1 (en) | 2014-06-11 | 2015-12-17 | Institut National De La Recherche Agronomique | Improved lipid accumulation in yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof |
| FR3028527A1 (fr) | 2014-11-13 | 2016-05-20 | Pivert | Identification de facteurs de transcription de yarrowia lipolytica |
| WO2016075314A1 (fr) | 2014-11-13 | 2016-05-19 | Institut National De La Recherche Agronomique | Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines |
| EP3085788A1 (de) | 2015-04-23 | 2016-10-26 | Institut National De La Recherche Agronomique | Mutanter hefestamm mit fähigkeit zum abbau von cellobiose |
| EP3106520A1 (de) | 2015-06-17 | 2016-12-21 | Institut National De La Recherche Agronomique | Mutanter yarrowia-stamm mit fähigkeit zum abbau von galaktose |
| CA3023338A1 (en) | 2016-05-10 | 2017-11-16 | Institut National De La Recherche Agronomique | Mutant yeast strains with enhanced production of erythritol or erythrulose |
| EP3348647A1 (de) | 2017-01-13 | 2018-07-18 | Institut National De La Recherche Agronomique | Mutanter hefestamm mit fähigkeit zur produktion von fettsäuren mittlerer länge |
| EP3360956A1 (de) | 2017-02-10 | 2018-08-15 | Institut National De La Recherche Agronomique | Zum abbau von cellulose fähiger, mutanter hefestamm |
| AU2022340887A1 (en) * | 2021-09-01 | 2024-03-14 | Chr. Hansen A/S | Yarrowia lipolytica and its use for producing lipases specifically liberating short chain fatty acids |
-
2000
- 2000-04-28 AT AT00925351T patent/ATE374823T1/de active
- 2000-04-28 AU AU2000244105A patent/AU2000244105A1/en not_active Abandoned
- 2000-04-28 DE DE60036647T patent/DE60036647T2/de not_active Expired - Lifetime
- 2000-04-28 WO PCT/FR2000/001148 patent/WO2001083773A1/fr active IP Right Grant
- 2000-04-28 PT PT00925351T patent/PT1276874E/pt unknown
- 2000-04-28 DE DE0001276874T patent/DE00925351T1/de active Pending
- 2000-04-28 EP EP00925351A patent/EP1276874B1/de not_active Expired - Lifetime
- 2000-04-28 ES ES00925351T patent/ES2200728T3/es not_active Expired - Lifetime
- 2000-04-28 DK DK00925351T patent/DK1276874T3/da active
- 2000-07-24 AT AT00946470T patent/ATE375525T1/de not_active IP Right Cessation
-
2003
- 2003-07-22 HK HK03105294.9A patent/HK1053145B/zh unknown
-
2008
- 2008-01-02 CY CY20081100005T patent/CY1107119T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1276874A1 (de) | 2003-01-22 |
| EP1276874B1 (de) | 2007-10-03 |
| ATE375525T1 (de) | 2007-10-15 |
| DE60036647T2 (de) | 2008-07-17 |
| AU2000244105A1 (en) | 2001-11-12 |
| DE00925351T1 (de) | 2004-04-15 |
| ES2200728T1 (es) | 2004-03-16 |
| DK1276874T3 (da) | 2008-02-04 |
| PT1276874E (pt) | 2008-01-11 |
| ATE374823T1 (de) | 2007-10-15 |
| CY1107119T1 (el) | 2012-10-24 |
| ES2200728T3 (es) | 2008-04-16 |
| HK1053145B (zh) | 2008-02-15 |
| HK1053145A1 (en) | 2003-10-10 |
| WO2001083773A1 (fr) | 2001-11-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |