HK1053145A1 - Cloning and expressing an acid-resistant extracel lular lipase of yarrowia lipolytica - Google Patents

Cloning and expressing an acid-resistant extracel lular lipase of yarrowia lipolytica

Info

Publication number
HK1053145A1
HK1053145A1 HK03105294A HK03105294A HK1053145A1 HK 1053145 A1 HK1053145 A1 HK 1053145A1 HK 03105294 A HK03105294 A HK 03105294A HK 03105294 A HK03105294 A HK 03105294A HK 1053145 A1 HK1053145 A1 HK 1053145A1
Authority
HK
Hong Kong
Prior art keywords
acid
resistant
lipase
cloning
expressing
Prior art date
Application number
HK03105294A
Other versions
HK1053145B (en
Inventor
Michel Seman
Georges Pignede
Frank Fudalej
Jean-Marc Nicaud
Claude Gaillardin
Original Assignee
Mayoly Spindler Lab
Frmichel Seman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mayoly Spindler Lab, Frmichel Seman filed Critical Mayoly Spindler Lab
Publication of HK1053145A1 publication Critical patent/HK1053145A1/en
Publication of HK1053145B publication Critical patent/HK1053145B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fertilizers (AREA)

Abstract

The invention concerns nucleic acids coding for acid-resistant extracellular lipases, in particular <i>C.ernobii</i> or <i>Y. lipolytica</i> yeasts and the production of said lipases in recombinant form.
HK03105294.9A 2000-04-28 2003-07-22 Cloning and expressing an acid-resistant extracellular lipase of yarrowia lipolytica HK1053145B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/FR2000/001148 WO2001083773A1 (en) 2000-04-28 2000-04-28 Cloning and expressing an acid-resistant extracellular lipase of yarrowia lipolytica

Publications (2)

Publication Number Publication Date
HK1053145A1 true HK1053145A1 (en) 2003-10-10
HK1053145B HK1053145B (en) 2008-02-15

Family

ID=8846450

Family Applications (1)

Application Number Title Priority Date Filing Date
HK03105294.9A HK1053145B (en) 2000-04-28 2003-07-22 Cloning and expressing an acid-resistant extracellular lipase of yarrowia lipolytica

Country Status (10)

Country Link
EP (1) EP1276874B1 (en)
AT (2) ATE374823T1 (en)
AU (1) AU2000244105A1 (en)
CY (1) CY1107119T1 (en)
DE (2) DE60036647T2 (en)
DK (1) DK1276874T3 (en)
ES (1) ES2200728T3 (en)
HK (1) HK1053145B (en)
PT (1) PT1276874E (en)
WO (1) WO2001083773A1 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7459546B2 (en) 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
WO2006039541A2 (en) 2004-09-30 2006-04-13 Novozymes, Inc. Polypeptides having lipase activity and polynucleotides encoding same
US7588931B2 (en) 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
KR100644924B1 (en) * 2005-04-15 2006-11-10 한국해양연구원 Ofloxacin ester-enantioselective lipase nucleotide encoding the lipase and method of producing the levofloxacin
CN100424170C (en) * 2005-10-11 2008-10-08 北京化工大学 Lipase, its gene, yalulipolytic geast for producing said enzyme and its application
CN102703404B (en) * 2006-06-15 2015-06-10 法国米奥里大药厂 Method for producing lipase, transformed Yarrowia lipolytica cell capable of producing lipase and their uses
JP5592648B2 (en) * 2006-06-15 2014-09-17 ラボラトワール マヨリ スパンドレ Method for producing lipase, transformed Yarrowia politica cells capable of producing said lipase and use thereof
FR2927089B1 (en) 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra METHOD OF TARGETED INTEGRATION OF MULTICOPIES OF A GENE OF INTEREST IN A YARROWIA STRAIN
DK2310517T3 (en) 2008-07-11 2016-06-06 Inst Nat De La Rech Agronomique (Inra) Novel mutant yeast strains having a capability to accumulate a large amount of lipids
FR2962133B1 (en) 2010-07-01 2014-09-12 Agronomique Inst Nat Rech OPTIMIZATION OF THE SYNTHESIS AND ACCUMULATION OF LIPIDS
FR3005317B1 (en) 2013-05-02 2016-03-18 Agronomique Inst Nat Rech MUTANT YEAS CAPABLE OF PRODUCING UNUSUAL FATTY ACID
WO2015189352A1 (en) 2014-06-11 2015-12-17 Institut National De La Recherche Agronomique Improved lipid accumulation in yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof
FR3028527A1 (en) 2014-11-13 2016-05-20 Pivert IDENTIFICATION OF TRANSCRIPTION FACTORS OF YARROWIA LIPOLYTICA
WO2016075314A1 (en) 2014-11-13 2016-05-19 Institut National De La Recherche Agronomique Identification of yarrowia lipolytica transcription factors that affect protein production
EP3085788A1 (en) 2015-04-23 2016-10-26 Institut National De La Recherche Agronomique Mutant yeast strain capable of degrading cellobiose
EP3106520A1 (en) 2015-06-17 2016-12-21 Institut National De La Recherche Agronomique Mutant yarrowia strain capable of degrading galactose
CA3023338A1 (en) 2016-05-10 2017-11-16 Institut National De La Recherche Agronomique Mutant yeast strains with enhanced production of erythritol or erythrulose
EP3348647A1 (en) 2017-01-13 2018-07-18 Institut National De La Recherche Agronomique Mutant yeast strain capable of producing medium chain fatty acids
EP3360956A1 (en) 2017-02-10 2018-08-15 Institut National De La Recherche Agronomique Mutant yeast strain capable of degrading cellulose
AU2022340887A1 (en) * 2021-09-01 2024-03-14 Chr. Hansen A/S Yarrowia lipolytica and its use for producing lipases specifically liberating short chain fatty acids

Also Published As

Publication number Publication date
EP1276874A1 (en) 2003-01-22
EP1276874B1 (en) 2007-10-03
ATE375525T1 (en) 2007-10-15
DE60036647T2 (en) 2008-07-17
AU2000244105A1 (en) 2001-11-12
DE00925351T1 (en) 2004-04-15
ES2200728T1 (en) 2004-03-16
DK1276874T3 (en) 2008-02-04
PT1276874E (en) 2008-01-11
ATE374823T1 (en) 2007-10-15
CY1107119T1 (en) 2012-10-24
ES2200728T3 (en) 2008-04-16
HK1053145B (en) 2008-02-15
DE60036647D1 (en) 2007-11-15
WO2001083773A1 (en) 2001-11-08

Similar Documents

Publication Publication Date Title
HK1053145A1 (en) Cloning and expressing an acid-resistant extracel lular lipase of yarrowia lipolytica
WO2005007818A3 (en) Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof
TW200611910A (en) Interferon-alpha polypeptides and conjugates
AU7503901A (en) Improved nucleic acid modifying enzymes
WO2006083276A3 (en) Interferon-alpha polypeptides and conjugates
BR0101847B1 (en) fuel tank and method of production thereof.
WO2001083770A3 (en) Lipolytic enzyme variant
ID29739A (en) COMPOSITE SHEETS THAT CAN BE BEGAN ELASTICALLY AND THE PROCESS OF MAKING IT
AU2433901A (en) Chimeric natriuretic peptides
MXPA04004266A (en) Use of il-19, il-22 and il-24 to treat hematopoietic disorders.
WO2000006718A3 (en) Expression of functional eukaryotic proteins
DE69830066D1 (en) Lunasin peptide
IL200132A0 (en) Method for cell adhesion and wound healing
ID29003A (en) COMBINED SHEETS THAT CAN STRETCH ELASTICALLY AND THE PROCESS OF MAKING IT
WO1999004011A3 (en) Hiv-1 group o antigens and uses thereof
MXPA02011232A (en) Directed evolution of oxidase enzymes.
WO2001074858A3 (en) Methods for altering t cell activation
DK0866848T3 (en) Enhanced neurospora hosts for the production of recombinant proteins and methods for producing the same
AU6829601A (en) Fatty acid elongase 3-ketoacyl coa synthase polypeptides
ZA963900B (en) Use of mannasases as slime control agents
WO2002095127A3 (en) Use of lipolytic enzymes for stickies control
WO2002008432A3 (en) Composition and method for increased meiotic recombination in plants
WO2003059932A3 (en) Methods of inducing or enhancing connective tissue repair
WO2001064912A3 (en) Triazine degrading enzymes
WO2002048187A3 (en) Secretion signal peptides, their dna sequences, expression vectors for eukaryotic cells that can be produced with the same, and use thereof for biotechnological production of proteins