WO2000006718A3 - Expression of functional eukaryotic proteins - Google Patents
Expression of functional eukaryotic proteins Download PDFInfo
- Publication number
- WO2000006718A3 WO2000006718A3 PCT/US1999/017127 US9917127W WO0006718A3 WO 2000006718 A3 WO2000006718 A3 WO 2000006718A3 US 9917127 W US9917127 W US 9917127W WO 0006718 A3 WO0006718 A3 WO 0006718A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- improved
- expression
- enzymes
- relates
- eukaryotic proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
- G01N2333/80—Cytochromes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90245—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
Abstract
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000562500A JP2003503005A (en) | 1998-07-28 | 1999-07-28 | Expression of functional eukaryotic proteins |
MXPA01000224A MXPA01000224A (en) | 1998-07-28 | 1999-07-28 | Expression of functional eukaryotic proteins. |
IL14050999A IL140509A0 (en) | 1998-07-28 | 1999-07-28 | Expression of functional eukaryotic proteins |
AU51345/99A AU5134599A (en) | 1998-07-28 | 1999-07-28 | Expression of functional eukaryotic proteins |
KR1020017000036A KR20010083086A (en) | 1998-07-28 | 1999-07-28 | Expression of Functional Eukaryotic Proteins |
EP99935983A EP1100891A2 (en) | 1998-07-28 | 1999-07-28 | Expression of functional eukaryotic proteins |
CA002331777A CA2331777A1 (en) | 1998-07-28 | 1999-07-28 | Expression of functional eukaryotic proteins |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9440398P | 1998-07-28 | 1998-07-28 | |
US60/094,403 | 1998-07-28 | ||
US24723299A | 1999-02-09 | 1999-02-09 | |
US09/247,232 | 1999-02-09 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2000006718A2 WO2000006718A2 (en) | 2000-02-10 |
WO2000006718A3 true WO2000006718A3 (en) | 2000-06-15 |
Family
ID=26788829
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1999/017127 WO2000006718A2 (en) | 1998-07-28 | 1999-07-28 | Expression of functional eukaryotic proteins |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1100891A2 (en) |
JP (1) | JP2003503005A (en) |
KR (1) | KR20010083086A (en) |
AU (1) | AU5134599A (en) |
CA (1) | CA2331777A1 (en) |
IL (1) | IL140509A0 (en) |
MX (1) | MXPA01000224A (en) |
WO (1) | WO2000006718A2 (en) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
AU1124499A (en) | 1997-10-28 | 1999-05-17 | Maxygen, Inc. | Human papillomavirus vectors |
EP1030861A4 (en) | 1997-10-31 | 2001-09-05 | Maxygen Inc | Modification of virus tropism and host range by viral genome shuffling |
US6902918B1 (en) | 1998-05-21 | 2005-06-07 | California Institute Of Technology | Oxygenase enzymes and screening method |
JP2002517995A (en) | 1998-06-17 | 2002-06-25 | マキシジェン, インコーポレイテッド | Method for producing a polynucleotide having desired properties |
WO2001072999A1 (en) * | 2000-03-27 | 2001-10-04 | California Institute Of Technology | Expression of functional eukaryotic proteins |
FR2810339B1 (en) * | 2000-06-14 | 2004-12-10 | Hoechst Marion Roussel Inc | COMBINATORIAL BANKS IMPROVED BY RECOMBINATION IN YEAST AND METHOD OF ANALYSIS |
JP2002199890A (en) * | 2000-10-23 | 2002-07-16 | Inst Of Physical & Chemical Res | Method for modifying biodegradable polyester synthetase |
US7465567B2 (en) | 2001-04-16 | 2008-12-16 | California Institute Of Technology | Peroxide-driven cytochrome P450 oxygenase variants |
WO2003008563A2 (en) | 2001-07-20 | 2003-01-30 | California Institute Of Technology | Improved cytochrome p450 oxygenases |
EP1639091B1 (en) | 2003-06-17 | 2012-12-05 | California University Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome p450 |
WO2005017116A2 (en) | 2003-08-11 | 2005-02-24 | California Institute Of Technology | Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use |
US11214817B2 (en) | 2005-03-28 | 2022-01-04 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US8715988B2 (en) | 2005-03-28 | 2014-05-06 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US8252559B2 (en) | 2006-08-04 | 2012-08-28 | The California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
US8026085B2 (en) | 2006-08-04 | 2011-09-27 | California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
US8802401B2 (en) | 2007-06-18 | 2014-08-12 | The California Institute Of Technology | Methods and compositions for preparation of selectively protected carbohydrates |
US9322007B2 (en) | 2011-07-22 | 2016-04-26 | The California Institute Of Technology | Stable fungal Cel6 enzyme variants |
US9914770B2 (en) * | 2013-04-30 | 2018-03-13 | Intas Pharmaceuticals Ltd | Cloning, expression and purification method for the preparation of ranibizumab |
EP3724212A4 (en) * | 2018-01-19 | 2021-11-24 | OBI Pharma, Inc. | Crm197 protein expression |
CN112852800B (en) * | 2020-01-09 | 2023-02-03 | 中国科学院天津工业生物技术研究所 | Signal peptide mutant and application thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1989003424A1 (en) * | 1987-10-08 | 1989-04-20 | British Bio-Technology Limited | Synthetic gene |
WO1997020078A1 (en) * | 1995-11-30 | 1997-06-05 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
WO1998006283A1 (en) * | 1996-08-14 | 1998-02-19 | Elisabeth Aguilar | Apron |
-
1999
- 1999-07-28 WO PCT/US1999/017127 patent/WO2000006718A2/en not_active Application Discontinuation
- 1999-07-28 MX MXPA01000224A patent/MXPA01000224A/en unknown
- 1999-07-28 KR KR1020017000036A patent/KR20010083086A/en not_active Application Discontinuation
- 1999-07-28 EP EP99935983A patent/EP1100891A2/en not_active Withdrawn
- 1999-07-28 IL IL14050999A patent/IL140509A0/en unknown
- 1999-07-28 AU AU51345/99A patent/AU5134599A/en not_active Abandoned
- 1999-07-28 JP JP2000562500A patent/JP2003503005A/en not_active Withdrawn
- 1999-07-28 CA CA002331777A patent/CA2331777A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1989003424A1 (en) * | 1987-10-08 | 1989-04-20 | British Bio-Technology Limited | Synthetic gene |
WO1997020078A1 (en) * | 1995-11-30 | 1997-06-05 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
WO1998006283A1 (en) * | 1996-08-14 | 1998-02-19 | Elisabeth Aguilar | Apron |
Non-Patent Citations (10)
Title |
---|
A. KUCHNER AND F.H. ARNOLD: "Directed evolution of enzyme catalysts", TREND IN BIOTECHNOLOGY, vol. 15, no. 12, December 1997 (1997-12-01), ELSEVIER SCIENCE LTD., AMSTERDAM, NL, pages 523 - 530, XP002133872 * |
CRAMERI A ET AL: "IMPROVED GREEN FLUORESCENT PROTEIN BY MOLECULAR EVOLUTION USING DNA SHUFFLING", NATURE BIOTECHNOLOGY,US,NATURE PUBLISHING, vol. 14, 14 March 1996 (1996-03-14), pages 315 - 319, XP000791095, ISSN: 1087-0156 * |
F.H. ARNOLD AND J.C. MOORE: "Optimizing industrial enzymes by directed evolution", ADV. BIOCHEM. ENG. BIOTECHNOL., vol. 58, 1997, SPRINGER-VERLAG, BERLIN HEIDELBERG, FRG, pages 1 - 14, XP000891392 * |
H. ZHAO AND F.H. ARNOLD: "Combinatorial protein design: strategies for screening protein libraries", CURRENT OPINION IN STRUCTURAL BIOLOGY, vol. 7, 1997, CURRENT BIOLOGY, PHILADELPHIA,US, pages 480 - 485, XP002133873 * |
J.C. MOORE ET AL.: "Strategies for the in vitro evolutilon of protein function: enzyme evolution by random recombination of improved sequences", J. MOL. BIOL., vol. 272, no. 3, 26 September 1997 (1997-09-26), ACADEMIC PRESS,US, pages 336 - 347, XP000892797 * |
JOO H ET AL: "A high-throughput digital imaging screen for the discovery and directed evolution of oxygenases.", CHEMISTRY AND BIOLOGY, (1999 OCT) 6 (10) 699-706. JOURNAL CODE: CNA., 10 September 1999 (1999-09-10), XP000892823 * |
JOO H ET AL: "Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation [see comments].", NATURE, (1999 JUN 17) 399 (6737) 670-3. JOURNAL CODE: NSC., XP002133874 * |
LIN Z ET AL: "Functional expression of horseradish peroxidase in bacteria through directed evolution.", 217TH NATIONAL MEETING OF THE AMERICAN CHEMICAL SOCIETY;ANAHEIM, CALIFORNIA, USA; MARCH 21-25, 1999, vol. 217, no. 1-2, 1999, Abstracts of Papers American Chemical Society 1999, pages BIOT 079, XP000891382, ISSN: 0065-7727 * |
LIN Z ET AL: "Functional expression of horseradish peroxidase in E. coli by directed evolution", BIOTECHNOLOGY PROGRESS, (1999 MAY-JUN) 15 (3) 467-71. JOURNAL CODE: ALG., XP000892792 * |
S.A. ORTLEPP ET AL.: "Expression and characterisation of a protein specified by a synthetic horseradish peroxidase gene in Escherichia coli", J. BIOTECHNOLOGY, vol. 11, 1989, ELSEVIER, AMSTERDAM,NL, pages 353 - 364, XP002133871 * |
Also Published As
Publication number | Publication date |
---|---|
AU5134599A (en) | 2000-02-21 |
MXPA01000224A (en) | 2002-10-17 |
KR20010083086A (en) | 2001-08-31 |
EP1100891A2 (en) | 2001-05-23 |
JP2003503005A (en) | 2003-01-28 |
CA2331777A1 (en) | 2000-02-10 |
WO2000006718A2 (en) | 2000-02-10 |
IL140509A0 (en) | 2002-02-10 |
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