ES2195383T3 - Procedimiento de lisis en un microorganismo. - Google Patents

Procedimiento de lisis en un microorganismo.

Info

Publication number
ES2195383T3
ES2195383T3 ES98942953T ES98942953T ES2195383T3 ES 2195383 T3 ES2195383 T3 ES 2195383T3 ES 98942953 T ES98942953 T ES 98942953T ES 98942953 T ES98942953 T ES 98942953T ES 2195383 T3 ES2195383 T3 ES 2195383T3
Authority
ES
Spain
Prior art keywords
container
alpha
biological sample
particulate material
organism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98942953T
Other languages
English (en)
Inventor
Lyse Santoro
Bruno Colin
Sophie Lopez
Cecile Paris
Corinne Jay
Dickey Kathleen Ann Clark
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Gen Probe Inc
Original Assignee
Biomerieux SA
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA, Gen Probe Inc filed Critical Biomerieux SA
Application granted granted Critical
Publication of ES2195383T3 publication Critical patent/ES2195383T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Procedimiento de lisis de una muestra biológica que contiene por lo menos un microorganismo de tipo bacteria, para liberar por lo menos un material nucleico de interés perteneciente a dicho microorganismo, según el cual: - se introduce dicha muestra biológica en medio líquido en un contenedor, - se introduce en dicho contenedor por lo menos un material constituido por partículas, relativamente duro y fundamentalmente inerte con respecto al material nucleico, - se somete la mezcla de la muestra biológica y el material constituido por partículas a un movimiento, caracterizado porque, en combinación: el movimiento elegido es de tipo vórtice y se ajusta a las características siguientes: - el material constituido por partículas está formado por bolas de diámetro comprendido entre 90 y 150 µm y - el volumen aparente de las bolas, Vb, y el volumen de la muestra líquida, Ve, guardan entre sí la relación siguiente: Ve = .Vb, en la que adopta un valor comprendido entre 1, 4 y 10 cuando el contenedor tiene forma tubular, mientras que es menor o igual a 2, 1 cuando el contenedor tiene forma de disco, mediante el cual y sin intervención de ningún otro reactivo ni operación suplementaria se cede directamente al medio líquido el material nucleico en estado nativo y accesible a cualquier reactivo.
ES98942953T 1997-09-23 1998-09-23 Procedimiento de lisis en un microorganismo. Expired - Lifetime ES2195383T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9712164A FR2768743B1 (fr) 1997-09-23 1997-09-23 Procede de lyse de micro-organisme

Publications (1)

Publication Number Publication Date
ES2195383T3 true ES2195383T3 (es) 2003-12-01

Family

ID=9511641

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98942953T Expired - Lifetime ES2195383T3 (es) 1997-09-23 1998-09-23 Procedimiento de lisis en un microorganismo.

Country Status (10)

Country Link
US (1) US6660472B1 (es)
EP (1) EP1017784B1 (es)
JP (2) JP4391017B2 (es)
AT (1) ATE238411T1 (es)
AU (1) AU728694B2 (es)
CA (1) CA2304846C (es)
DE (1) DE69813848T2 (es)
ES (1) ES2195383T3 (es)
FR (1) FR2768743B1 (es)
WO (1) WO1999015621A1 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2780059B1 (fr) 1998-06-17 2002-10-11 Bio Merieux Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus
FR2781500B1 (fr) 1998-07-23 2000-09-08 Bio Merieux Dispositif perfectionne et procede de lyse de micro-organismes
IL149704A0 (en) 1999-12-14 2002-11-10 Novartis Ag Bovine immunodeficiency virus (biv) based vectors
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
FR2812303B1 (fr) * 2000-07-27 2002-12-06 Biomerieux Sa Procede de lyse cellulaire de procaryotes ou d'eucaryotes ou de lyse cellulaire simultanee de procaryotes et d'eucaryotes
US7338805B2 (en) 2001-05-04 2008-03-04 Bio Merieux Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
US8501402B2 (en) * 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
FR2868071B1 (fr) 2004-03-26 2006-06-09 Biomerieux Sa Reactifs de marquage, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
FR2873388B1 (fr) * 2004-07-23 2012-04-06 Biomerieux Sa Procede de marquage et de purification d'acides nucleiques d'interet presents dans un echantillon biologique a traiter dans un unique recipient reactionnel
US7592139B2 (en) 2004-09-24 2009-09-22 Sandia National Laboratories High temperature flow-through device for rapid solubilization and analysis
JP2006141292A (ja) * 2004-11-19 2006-06-08 Amr Kk 微生物の破砕・核酸抽出方法、この方法を用いたキット、及びその製造方法
FR2886735B1 (fr) 2005-06-01 2015-09-11 Biomerieux Sa Procede de marquage ou de traitement d'un echantillon biologique contenant des molecules biologiques d'interet, notamment des acides nucleiques
FR2902430B1 (fr) 2005-11-25 2012-11-02 Biomerieux Sa Oligonucleotides, utilisation, methode de detection et kit permettant de diagnostiquer la presence des genes h5 et n1 du virus d'influenza a
KR100829585B1 (ko) * 2006-04-07 2008-05-14 삼성전자주식회사 표적 세포 분리 및 신속한 핵산 분리 방법 및 장치
FR2917090B1 (fr) * 2007-06-11 2012-06-15 Biomerieux Sa Reactifs de marquage portant des fonctions diazo et nitro, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
FR2921064A1 (fr) * 2007-09-14 2009-03-20 Biomerieux Sa Oligonucleotides, utilisation, methode de detection et kit permettant de diagnostiquer la presence du gene e1 du virus du chikungunya
FR2934595B1 (fr) 2008-07-29 2013-04-05 Biomerieux Sa Reactifs de marquage ayant un noyau pyridine portant une fonction diazomethyle, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
CN102105602A (zh) * 2008-07-30 2011-06-22 通用电气公司 评价致病两期细菌的毒力的方法
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US8986986B2 (en) 2010-10-29 2015-03-24 Samsung Electronics Co., Ltd. Cell lysis device and methods of lysing cells or viruses
CN107099445B (zh) 2012-03-16 2020-06-16 统计诊断与创新有限公司 具有集成传送模块的测试盒

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL6811627A (es) * 1967-08-18 1969-02-20
US4295613A (en) * 1979-10-03 1981-10-20 Vpi Educational Foundation Apparatus for breaking bacterial cells
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US4666850A (en) * 1983-10-28 1987-05-19 Becton, Dickinson And Company Microbial pathogen detecting system and process
CA1296604C (en) * 1986-03-20 1992-03-03 Kathleen Murphy Method for releasing rna and dna from cells
ATE103333T1 (de) * 1987-03-23 1994-04-15 Zymogenetics Inc Hohe proteinsyntheserate in hefe.
US4873035A (en) * 1987-11-25 1989-10-10 Abbott Laboratories Preparation of sized populations of liposomes
AU614121B2 (en) * 1988-05-04 1991-08-22 Novartis Ag Improvements in the production of polypeptides
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
JPH0817701B2 (ja) * 1990-03-23 1996-02-28 キッコーマン株式会社 アルカリプロテアーゼ、アルカリプロテアーゼ遺伝子、新規な組み換え体ｄｎａ、アルカリプロテアーゼの製造法、及び遺伝子発現用ｄｎａ断片
FR2707010B1 (es) 1993-06-25 1995-09-29 Bio Merieux
US5464773A (en) * 1994-03-14 1995-11-07 Amoco Corporation Cell disrupting apparatus
US5643767A (en) * 1994-05-02 1997-07-01 The Rockefeller University Process for isolating cellular components
US5620869A (en) 1995-09-28 1997-04-15 Becton, Dickinson And Company Methods for reducing inhibition of nucleic acid amplification reactions
US5707860A (en) * 1996-03-12 1998-01-13 Becton Dickinson And Company Vehicle for delivery of particles to a sample
ES2136458T3 (es) * 1996-06-14 1999-11-16 Takeda Chemical Industries Ltd Metodo para separar la metionina n-terminal.

Also Published As

Publication number Publication date
CA2304846A1 (fr) 1999-04-01
ATE238411T1 (de) 2003-05-15
JP4391017B2 (ja) 2009-12-24
JP2009131274A (ja) 2009-06-18
JP2002512001A (ja) 2002-04-23
EP1017784B1 (fr) 2003-04-23
CA2304846C (fr) 2012-04-24
FR2768743A1 (fr) 1999-03-26
DE69813848D1 (de) 2003-05-28
JP4422782B2 (ja) 2010-02-24
DE69813848T2 (de) 2004-02-26
FR2768743B1 (fr) 2001-09-14
US6660472B1 (en) 2003-12-09
AU9091298A (en) 1999-04-12
EP1017784A1 (fr) 2000-07-12
AU728694B2 (en) 2001-01-18
WO1999015621A1 (fr) 1999-04-01

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