YU23601A - In vitro ogled na humani hepatitis b virus (hbv) dna polimeraze, i njegova upotreba za odredjivanje inhibitora hbv dna polimeraze - Google Patents
In vitro ogled na humani hepatitis b virus (hbv) dna polimeraze, i njegova upotreba za odredjivanje inhibitora hbv dna polimerazeInfo
- Publication number
- YU23601A YU23601A YU23601A YU23601A YU23601A YU 23601 A YU23601 A YU 23601A YU 23601 A YU23601 A YU 23601A YU 23601 A YU23601 A YU 23601A YU 23601 A YU23601 A YU 23601A
- Authority
- YU
- Yugoslavia
- Prior art keywords
- dna polymerase
- hbv
- virus
- vitro activity
- assay
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Dati pronalazak obezbedjuje ogled za odredjivanje in vitro aktivnosti DNA polimeraze humanog virusa hepatitisa B (HBV), koji obuhvata korišćenje 5'-oligonukleotida 5' oligonukleotida kao prajmera za umnožavanje HBV DNA polimeraze PCR reakcijom iz uzorka, oligonukleotida u koji je umetnut promoter SP6 virusne polimeraze, direktno transkribujući i prepisujući PCR proizvode u prisustvu radioaktivno obeleženog sredstva i merenje (polimerizujuće) aktivnosti HBV DNA polimeraze. Ovaj pronalazak takodje obezbedjuje upotrebu takvog ogleda da bi se ispitala aktivnost različitih uzoraka seruma, za odredjivanje inhibitora HBV DNA polimeraze i za testiranje /ili odredjivanje potencijalnih lekova protiv HBV za njiihovu sposobnost da inhibiraju (polimerizujuće) aktivnosti DNA za HBV DNA polimerazu.[The present invention provides an in vitro activity assay for human hepatitis B virus (HBV) DNA polymerase, which comprises using, as the 5' oligonucleotide in PCR amplification of HBV DNA polymerase from a sample, an oligonucleotide into which has been incorporated the SP6 viral polymerase promoter, directly transcribing and translating the PCR products in the presence of a radio- labelled agent and measuring the priming of the HBV DNA polymerase. The present invention also provides the use of such an assay to assay activity of various serum samples, to screen for inhibitors of the HBV DNA polymerase and to test and/or screen potential anti- HBV drugs for their ability to inhibit DNA priming activity of human HBV DNA polymerase.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/SG1998/000077 WO2000018958A2 (en) | 1998-09-29 | 1998-09-29 | An in vitro activity assay for human hepatits b virus (hbv) dna polymerase, the use of such an assay to assay activity of various serum samples and to screen for inhibitors of the hbv dna polymerase and a method of producing hbv dna polymerase |
Publications (1)
Publication Number | Publication Date |
---|---|
YU23601A true YU23601A (sh) | 2005-07-19 |
Family
ID=20429877
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
YU23601A YU23601A (sh) | 1998-09-29 | 1999-03-13 | In vitro ogled na humani hepatitis b virus (hbv) dna polimeraze, i njegova upotreba za odredjivanje inhibitora hbv dna polimeraze |
Country Status (23)
Country | Link |
---|---|
US (1) | US6593082B1 (sh) |
EP (1) | EP1117843A1 (sh) |
JP (1) | JP2002525130A (sh) |
KR (1) | KR100445958B1 (sh) |
CN (1) | CN1197978C (sh) |
AR (1) | AR030012A1 (sh) |
AU (2) | AU9372698A (sh) |
BR (1) | BR9914099A (sh) |
CA (1) | CA2344282A1 (sh) |
HK (1) | HK1039161A1 (sh) |
HR (1) | HRP20010230B1 (sh) |
HU (1) | HUP0103735A3 (sh) |
IL (2) | IL142288A0 (sh) |
IS (1) | IS5900A (sh) |
MY (1) | MY123274A (sh) |
NO (1) | NO20011510L (sh) |
NZ (1) | NZ510704A (sh) |
PL (1) | PL347026A1 (sh) |
RU (1) | RU2202620C2 (sh) |
TR (1) | TR200100899T2 (sh) |
UA (1) | UA75028C2 (sh) |
WO (2) | WO2000018958A2 (sh) |
YU (1) | YU23601A (sh) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004508018A (ja) * | 2000-07-14 | 2004-03-18 | レプリダイン・インコーポレーテッド | 新規dnaポリメラーゼiiiホロ酵素デルタサブユニット核酸分子及び蛋白質 |
AUPS037002A0 (en) * | 2002-02-07 | 2002-02-28 | Melbourne Health | Viral variants and uses therefor |
KR20030075638A (ko) * | 2002-03-20 | 2003-09-26 | (주) 비엔씨바이오팜 | B형 간염바이러스의 프라이밍 저해제의 스크리닝 방법 |
TW200643171A (en) | 2005-06-07 | 2006-12-16 | Temasek Life Sciences Lab Ltd | Porcine circovirus type 2 vaccines |
US8859229B2 (en) | 2007-02-02 | 2014-10-14 | Yale University | Transient transfection with RNA |
US9249423B2 (en) | 2007-02-02 | 2016-02-02 | Yale University | Method of de-differentiating and re-differentiating somatic cells using RNA |
US10155038B2 (en) | 2007-02-02 | 2018-12-18 | Yale University | Cells prepared by transient transfection and methods of use thereof |
WO2011047312A1 (en) | 2009-10-16 | 2011-04-21 | Glaxo Group Limited | Hbv antisense inhibitors |
CN104316682B (zh) * | 2014-10-10 | 2015-12-30 | 三峡大学 | 一种s-腺苷甲硫氨酸脱羧酶的活性检测方法及其应用 |
RU2763173C1 (ru) * | 2020-07-27 | 2021-12-28 | Федеральное бюджетное учреждение науки "Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. Пастера Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека" (ФБУН НИИ эпидемиологии и микробиологии имени Пастера) | Способ выявления ДНК вируса гепатита В в биологическом материале при низкой вирусной нагрузке на основе двухэтапной ПЦР с детекцией по трем мишеням в режиме реального времени |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5492817A (en) * | 1993-11-09 | 1996-02-20 | Promega Corporation | Coupled transcription and translation in eukaryotic cell-free extract |
US5360714A (en) * | 1992-08-28 | 1994-11-01 | Fox Chase Cancer Center | Hepadnavirus polymerase gene product having RNA-dependent DNA priming and reverse transcriptase activities and methods of measuring the activities thereof |
WO1994005812A1 (en) * | 1992-09-02 | 1994-03-17 | The Scripps Research Institute | Coupled isothermal polynucleotide amplification and translation system |
US5709998A (en) * | 1993-12-15 | 1998-01-20 | The Johns Hopkins University | Molecular diagnosis of familial adenomatous polyposis |
WO1996010649A1 (en) * | 1994-09-30 | 1996-04-11 | Southwest Foundation For Biomedical Research | Isolated human hepatitis b virus polymerase and uses thereof |
-
1998
- 1998-09-29 WO PCT/SG1998/000077 patent/WO2000018958A2/en active Application Filing
- 1998-09-29 AU AU93726/98A patent/AU9372698A/en not_active Abandoned
-
1999
- 1999-03-13 CA CA002344282A patent/CA2344282A1/en not_active Abandoned
- 1999-03-13 TR TR2001/00899T patent/TR200100899T2/xx unknown
- 1999-03-13 UA UA2001032047A patent/UA75028C2/uk unknown
- 1999-03-13 BR BR9914099-3A patent/BR9914099A/pt not_active IP Right Cessation
- 1999-03-13 PL PL99347026A patent/PL347026A1/xx unknown
- 1999-03-13 RU RU2001108496/14A patent/RU2202620C2/ru not_active IP Right Cessation
- 1999-03-13 WO PCT/SG1999/000017 patent/WO2000018968A1/en active IP Right Grant
- 1999-03-13 CN CNB998115274A patent/CN1197978C/zh not_active Expired - Fee Related
- 1999-03-13 IL IL14228899A patent/IL142288A0/xx not_active IP Right Cessation
- 1999-03-13 HU HU0103735A patent/HUP0103735A3/hu unknown
- 1999-03-13 NZ NZ510704A patent/NZ510704A/xx unknown
- 1999-03-13 AU AU29694/99A patent/AU772819B2/en not_active Ceased
- 1999-03-13 KR KR10-2001-7004053A patent/KR100445958B1/ko not_active IP Right Cessation
- 1999-03-13 JP JP2000572415A patent/JP2002525130A/ja active Pending
- 1999-03-13 YU YU23601A patent/YU23601A/sh unknown
- 1999-03-13 US US09/674,465 patent/US6593082B1/en not_active Expired - Fee Related
- 1999-03-13 EP EP99910940A patent/EP1117843A1/en not_active Withdrawn
- 1999-09-24 MY MYPI99004150A patent/MY123274A/en unknown
- 1999-09-28 AR ARP990104895A patent/AR030012A1/es unknown
-
2001
- 2001-03-19 IS IS5900A patent/IS5900A/is unknown
- 2001-03-23 NO NO20011510A patent/NO20011510L/no unknown
- 2001-03-27 IL IL142288A patent/IL142288A/en unknown
- 2001-03-27 HR HR20010230A patent/HRP20010230B1/xx not_active IP Right Cessation
-
2002
- 2002-01-16 HK HK02100353.9A patent/HK1039161A1/zh unknown
Also Published As
Publication number | Publication date |
---|---|
WO2000018968A8 (en) | 2002-11-07 |
KR20010083879A (ko) | 2001-09-03 |
HRP20010230A2 (en) | 2002-06-30 |
AR030012A1 (es) | 2003-08-13 |
EP1117843A1 (en) | 2001-07-25 |
KR100445958B1 (ko) | 2004-08-25 |
HRP20010230B1 (en) | 2006-05-31 |
WO2000018968A1 (en) | 2000-04-06 |
IL142288A0 (en) | 2002-03-10 |
CN1197978C (zh) | 2005-04-20 |
US6593082B1 (en) | 2003-07-15 |
HUP0103735A3 (en) | 2005-12-28 |
IS5900A (is) | 2001-03-19 |
NO20011510L (no) | 2001-05-28 |
AU9372698A (en) | 2000-04-17 |
IL142288A (en) | 2006-12-31 |
CA2344282A1 (en) | 2000-04-06 |
CN1320167A (zh) | 2001-10-31 |
TR200100899T2 (tr) | 2001-07-23 |
AU2969499A (en) | 2000-04-17 |
JP2002525130A (ja) | 2002-08-13 |
HK1039161A1 (zh) | 2002-04-12 |
WO2000018958A2 (en) | 2000-04-06 |
HUP0103735A2 (hu) | 2002-01-28 |
AU772819B2 (en) | 2004-05-06 |
PL347026A1 (en) | 2002-03-11 |
NO20011510D0 (no) | 2001-03-23 |
BR9914099A (pt) | 2001-07-31 |
MY123274A (en) | 2006-05-31 |
NZ510704A (en) | 2002-10-25 |
UA75028C2 (en) | 2006-03-15 |
RU2202620C2 (ru) | 2003-04-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1026262A3 (en) | Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof | |
DE69503129D1 (de) | Pteridin-nukleotidderivate als fluoreszierende sonden | |
EP0776981A3 (en) | Oligonucleotide primers for amplifying HCV nucleic acid | |
PT1381697E (pt) | Sonda circular de amplificação (cpa) utilizando iniciadores de transferência de energia | |
ATE199158T1 (de) | Hbv amplifizierungssonden zur verwendung in hybridisierungssandwichassay in der lösungsphase | |
YU23601A (sh) | In vitro ogled na humani hepatitis b virus (hbv) dna polimeraze, i njegova upotreba za odredjivanje inhibitora hbv dna polimeraze | |
ES2195383T3 (es) | Procedimiento de lisis en un microorganismo. | |
AU3054502A (en) | Compositions and methods for detecting human immunodeficiency virus 2 (hiv-2) | |
FR2769315B1 (fr) | Conjugues fluorescents de nucleosides ou de nucleotides, leur procede de preparation et leur utilisation | |
GB8929293D0 (en) | C.difficile dna probes | |
RU2001108496A (ru) | АНАЛИЗ АКТИВНОСТИ in vitro ДЛЯ ДНК-ПОЛИМЕРАЗЫ ВИРУСА ГЕПАТИТА В (HBV) И ЕГО ИСПОЛЬЗОВАНИЕ ДЛЯ СКРИНИНГА НА ИНГИБИТОРЫ ДНК-ПОЛИМЕРАЗЫ HBV | |
EP1774048B1 (en) | Quantitative detection of hdv by real-time rt-pcr | |
EP0989192A3 (en) | Method for synthesis of nucleic acids | |
MY128619A (en) | Detection of human hepatitis b virus surface antigen mutants by specific amplification and its application on gene chip | |
DE60018926D1 (de) | "cort"-pcr assay zur unterscheidung von endogener reversen transkriptase aktivität in eukaryontischen zellen von einer kontamination durch infektiöse retroviren | |
Caliendo | Laboratory methods for quantitating HIV RNA | |
TH49471B (th) | การวิเคราะห์พฤติการณ์ดีเอนเอโพลีเมอเรสของไวรัสตับอักเสบชนิดบีในมนุษย์(hbv)ในหลอดแก้ว การใช้การวิเคราะห์เช่นว่านั้นเพื่อวิเคราะห์การทำงานของตัวอย่างเซรุ่มต่างๆ และเพื่อร่อนหาตัวยับยั้ง hbv dna โพลีเมอเรสและวิธีการผลิต hbv dna โพลีเมอเรส | |
Levin | A perspective on viral load testing | |
ATE414175T1 (de) | Detektion von rpob sequenzen von mycobacterium tuberculosis | |
ES2170620A1 (es) | Procedimiento de cuantificacion de acidos nucleicos mediante amplificacion por reaccion en cadena de polimerasa hemi-competitiva. | |
TH29524B (th) | น้ำยาตรวจหาไวรัสที่ใช้ปฏิกริยาเกาะกลุ่มของเชื้อไวรัสและชุดน้ำยาตรวจหาเชื้อไวรัส |