ES2187598T3 - Metodos para producir proteinas de fusion. - Google Patents
Metodos para producir proteinas de fusion.Info
- Publication number
- ES2187598T3 ES2187598T3 ES96113414T ES96113414T ES2187598T3 ES 2187598 T3 ES2187598 T3 ES 2187598T3 ES 96113414 T ES96113414 T ES 96113414T ES 96113414 T ES96113414 T ES 96113414T ES 2187598 T3 ES2187598 T3 ES 2187598T3
- Authority
- ES
- Spain
- Prior art keywords
- dna sequence
- methods
- transfected
- fusion proteins
- restriction site
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
- C07K16/462—Igs containing a variable region (Fv) from one specie and a constant region (Fc) from another
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
SE DESCRIBEN METODOS DE PRODUCCION DE PROTEINAS DE FUSION, INCLUYENDO AQUELLAS CON ACTIVIDADES BIOLOGICAS DUALES. DICHOS METODOS INCLUYEN: LA PROVISION DE UNA PRIMERA Y UNA SEGUNDA SECUENCIAS DE ADN, QUE CODIFICAN PARA UN PRIMER Y UN SEGUNDO POLIPEPTIDOS, RESPECTIVAMENTE; LA DIGESTION DE LA PRIMERA SECUENCIA DE ADN EN UN SITIO DE RESTRICCION ADYACENTE A SU EXTREMO 5''; Y LA UNION DE UNA SECUENCIA DE CONEXION/ADAPTADORA (1/A), AL EXTREMO DE RESTRICCION DE LA PRIMERA SECUENCIA DE ADN, FORMANDOSE POR TANTO UNA CASETE. LA 1/A INCLUYE, EN UN EXTREMO, LA PORCION DE LA PRIMERA SECUENCIA DE ADN QUE SE EXTIENDE DESDE SU EXTREMO MAS CERCANO AL SITIO DE RESTRICCION, HASTA EL SITIO DE RESTRICCION, Y, EN EL OTRO EXTREMO, UNA HEBRA DE UN SITIO DE RUPTURA. SE TRANSFORMA UNA CELULA HUESPED EUCARIOTICA, CON LA CITADA CASETE, Y LA SEGUNDA SECUENCIA DE ADN, QUE TIENE EN UN EXTREMO UNA HEBRA DEL SITIO DE RUPTURA, COMPATIBLE CON LA HEBRA DEL SITIO DE RUPTURA EN 1/A. LA CELULA HUESPED TRANSFORMADA, SE CULTIVA PARA EXPRESAR EL ADN TRANSFORMADO COMO UNA PROTEINA DE FUSION DE CADENA UNICA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US40988989A | 1989-09-20 | 1989-09-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2187598T3 true ES2187598T3 (es) | 2003-06-16 |
Family
ID=23622388
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96113414T Expired - Lifetime ES2187598T3 (es) | 1989-09-20 | 1990-08-01 | Metodos para producir proteinas de fusion. |
ES90913419T Expired - Lifetime ES2103274T3 (es) | 1989-09-20 | 1990-08-01 | Metodo de produccion de proteinas de fusion. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90913419T Expired - Lifetime ES2103274T3 (es) | 1989-09-20 | 1990-08-01 | Metodo de produccion de proteinas de fusion. |
Country Status (9)
Country | Link |
---|---|
EP (2) | EP0765937B1 (es) |
JP (1) | JP3051162B2 (es) |
AT (2) | ATE228566T1 (es) |
AU (1) | AU633357B2 (es) |
CA (1) | CA2066612C (es) |
DE (2) | DE69034022T2 (es) |
DK (2) | DK0493418T3 (es) |
ES (2) | ES2187598T3 (es) |
WO (1) | WO1991004329A1 (es) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5116964A (en) * | 1989-02-23 | 1992-05-26 | Genentech, Inc. | Hybrid immunoglobulins |
US6406697B1 (en) | 1989-02-23 | 2002-06-18 | Genentech, Inc. | Hybrid immunoglobulins |
US5225538A (en) * | 1989-02-23 | 1993-07-06 | Genentech, Inc. | Lymphocyte homing receptor/immunoglobulin fusion proteins |
US5498538A (en) * | 1990-02-15 | 1996-03-12 | The University Of North Carolina At Chapel Hill | Totally synthetic affinity reagents |
US5747334A (en) * | 1990-02-15 | 1998-05-05 | The University Of North Carolina At Chapel Hill | Random peptide library |
ATE220113T1 (de) * | 1990-10-22 | 2002-07-15 | Genentech Inc | Verfahren zur modifizierung von dna und zur detektion der wirkungen auf die interaktion von modifizierten polypeptiden und zielsubstraten |
US5525491A (en) * | 1991-02-27 | 1996-06-11 | Creative Biomolecules, Inc. | Serine-rich peptide linkers |
US6103521A (en) * | 1995-02-06 | 2000-08-15 | Cell Genesys, Inc. | Multispecific chimeric receptors |
US5723125A (en) * | 1995-12-28 | 1998-03-03 | Tanox Biosystems, Inc. | Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide |
WO2001004306A1 (en) * | 1999-07-12 | 2001-01-18 | Genentech, Inc. | Expression vectors and methods |
ATE316982T1 (de) | 1999-08-09 | 2006-02-15 | Lexigen Pharm Corp | Mehrere zytokin-antikörper komplexen |
US20030133939A1 (en) | 2001-01-17 | 2003-07-17 | Genecraft, Inc. | Binding domain-immunoglobulin fusion proteins |
US7754208B2 (en) | 2001-01-17 | 2010-07-13 | Trubion Pharmaceuticals, Inc. | Binding domain-immunoglobulin fusion proteins |
AU2002248571B2 (en) * | 2001-03-07 | 2007-01-18 | Merck Patent Gmbh | Expression technology for proteins containing a hybrid isotype antibody moiety |
WO2002079415A2 (en) | 2001-03-30 | 2002-10-10 | Lexigen Pharmaceuticals Corp. | Reducing the immunogenicity of fusion proteins |
JP4987484B2 (ja) | 2004-01-22 | 2012-07-25 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング | 補体結合が低下した抗癌抗体 |
EP1912675B1 (en) | 2005-07-25 | 2014-02-12 | Emergent Product Development Seattle, LLC | B-cell reduction using cd37-specific and cd20-specific binding molecules |
DK1966245T3 (da) | 2005-12-30 | 2011-07-18 | Merck Patent Gmbh | Anti-CD19-Antistoffer med reduceret immunogenicitet |
CN101351475B (zh) | 2005-12-30 | 2013-05-15 | 默克专利有限公司 | 具有提高的稳定性的白细胞介素12p40变体 |
US8409577B2 (en) | 2006-06-12 | 2013-04-02 | Emergent Product Development Seattle, Llc | Single chain multivalent binding proteins with effector function |
JP6013733B2 (ja) | 2008-04-11 | 2016-10-25 | エマージェント プロダクト デベロップメント シアトル, エルエルシー | Cd37免疫治療薬および二機能性化学療法薬とのその組合せ |
GB201403775D0 (en) | 2014-03-04 | 2014-04-16 | Kymab Ltd | Antibodies, uses & methods |
US10071748B2 (en) | 2015-09-17 | 2018-09-11 | Sony Corporation | System and method for providing driving assistance to safely overtake a vehicle |
KR20180053322A (ko) | 2015-09-21 | 2018-05-21 | 압테보 리서치 앤드 디벨롭먼트 엘엘씨 | Cd3 결합 폴리펩타이드 |
US9567399B1 (en) | 2016-06-20 | 2017-02-14 | Kymab Limited | Antibodies and immunocytokines |
EP3534947A1 (en) | 2016-11-03 | 2019-09-11 | Kymab Limited | Antibodies, combinations comprising antibodies, biomarkers, uses & methods |
CN110592057B (zh) * | 2019-09-27 | 2022-01-28 | 昆明理工大学 | 嵌合裂解酶ILTphg和编码此酶的多核苷酸 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4771002A (en) * | 1984-02-24 | 1988-09-13 | Lubrizol Genetics, Inc. | Transcription in plants and bacteria |
-
1990
- 1990-08-01 DK DK90913419.9T patent/DK0493418T3/da active
- 1990-08-01 EP EP96113414A patent/EP0765937B1/en not_active Expired - Lifetime
- 1990-08-01 ES ES96113414T patent/ES2187598T3/es not_active Expired - Lifetime
- 1990-08-01 DE DE69034022T patent/DE69034022T2/de not_active Expired - Fee Related
- 1990-08-01 AT AT96113414T patent/ATE228566T1/de not_active IP Right Cessation
- 1990-08-01 DE DE69030579T patent/DE69030579T2/de not_active Expired - Fee Related
- 1990-08-01 JP JP2512474A patent/JP3051162B2/ja not_active Expired - Fee Related
- 1990-08-01 CA CA002066612A patent/CA2066612C/en not_active Expired - Lifetime
- 1990-08-01 EP EP90913419A patent/EP0493418B1/en not_active Expired - Lifetime
- 1990-08-01 DK DK96113414T patent/DK0765937T3/da active
- 1990-08-01 ES ES90913419T patent/ES2103274T3/es not_active Expired - Lifetime
- 1990-08-01 AT AT90913419T patent/ATE152174T1/de not_active IP Right Cessation
- 1990-08-01 AU AU63352/90A patent/AU633357B2/en not_active Ceased
- 1990-08-01 WO PCT/US1990/004301 patent/WO1991004329A1/en active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
JPH05500310A (ja) | 1993-01-28 |
DE69034022T2 (de) | 2003-07-10 |
EP0765937A1 (en) | 1997-04-02 |
DK0765937T3 (da) | 2003-03-24 |
CA2066612C (en) | 1998-12-08 |
AU6335290A (en) | 1991-04-18 |
WO1991004329A1 (en) | 1991-04-04 |
EP0493418A4 (en) | 1992-09-02 |
DE69030579T2 (de) | 1997-11-13 |
DE69034022D1 (de) | 2003-01-09 |
ATE228566T1 (de) | 2002-12-15 |
JP3051162B2 (ja) | 2000-06-12 |
EP0765937B1 (en) | 2002-11-27 |
CA2066612A1 (en) | 1991-03-21 |
DE69030579D1 (de) | 1997-05-28 |
DK0493418T3 (da) | 1997-11-03 |
AU633357B2 (en) | 1993-01-28 |
EP0493418B1 (en) | 1997-04-23 |
EP0493418A1 (en) | 1992-07-08 |
ATE152174T1 (de) | 1997-05-15 |
ES2103274T3 (es) | 1997-09-16 |
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