ES2182736T1 - Procedimientos para el ensayo de la actividad receptora y las construcciones utiles en dichos procedimientos. - Google Patents

Procedimientos para el ensayo de la actividad receptora y las construcciones utiles en dichos procedimientos.

Info

Publication number
ES2182736T1
ES2182736T1 ES98925260T ES98925260T ES2182736T1 ES 2182736 T1 ES2182736 T1 ES 2182736T1 ES 98925260 T ES98925260 T ES 98925260T ES 98925260 T ES98925260 T ES 98925260T ES 2182736 T1 ES2182736 T1 ES 2182736T1
Authority
ES
Spain
Prior art keywords
procedures
methods
test
activity
gpcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES98925260T
Other languages
English (en)
Other versions
ES2182736T3 (es
Inventor
Lawrence S Barak
Marc G Caron
Stephen S Ferguson
Jie Zhang
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Duke University
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Duke University
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Filing date
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Application filed by Duke University filed Critical Duke University
Publication of ES2182736T1 publication Critical patent/ES2182736T1/es
Application granted granted Critical
Publication of ES2182736T3 publication Critical patent/ES2182736T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Conjugado que comprende una proteína -arrestina y una molécula detectable, en el que la molécula detectable es capaz de indicar la transposición intracelular y/o la distribución de la proteína -arrestina.
ES98925260T 1997-06-05 1998-06-04 Procedimientos de analisis de la actividad de receptores y constructos utiles en dichos procedimientos. Expired - Lifetime ES2182736T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US869568 1997-06-05
US08/869,568 US5891646A (en) 1997-06-05 1997-06-05 Methods of assaying receptor activity and constructs useful in such methods

Publications (2)

Publication Number Publication Date
ES2182736T1 true ES2182736T1 (es) 2003-03-16
ES2182736T3 ES2182736T3 (es) 2004-09-01

Family

ID=25353824

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98925260T Expired - Lifetime ES2182736T3 (es) 1997-06-05 1998-06-04 Procedimientos de analisis de la actividad de receptores y constructos utiles en dichos procedimientos.

Country Status (10)

Country Link
US (5) US5891646A (es)
EP (2) EP1441032B1 (es)
AT (2) ATE262040T1 (es)
AU (1) AU759347B2 (es)
CA (1) CA2305810C (es)
DE (3) DE1015608T1 (es)
DK (1) DK1015608T3 (es)
ES (1) ES2182736T3 (es)
PT (1) PT1015608E (es)
WO (1) WO1998055635A1 (es)

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US20080261256A1 (en) 2008-10-23
DE69822484T2 (de) 2005-01-13
EP1015608B1 (en) 2004-03-17
US20040101887A1 (en) 2004-05-27
US20060188944A1 (en) 2006-08-24
DE1015608T1 (de) 2002-11-14
WO1998055635A1 (en) 1998-12-10
EP1441032B1 (en) 2010-09-22
EP1015608A1 (en) 2000-07-05
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US5891646A (en) 1999-04-06
ATE482276T1 (de) 2010-10-15
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US7138240B2 (en) 2006-11-21
DE69841908D1 (de) 2010-11-04
ATE262040T1 (de) 2004-04-15
CA2305810A1 (en) 1998-12-10
CA2305810C (en) 2007-11-20
US6110693A (en) 2000-08-29
WO1998055635A9 (en) 2003-05-08
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