WO2006136824A3 - Ratiometric multicolour fluorescence correlation screening; agents for use therewith and methods of production thereof - Google Patents

Ratiometric multicolour fluorescence correlation screening; agents for use therewith and methods of production thereof Download PDF

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Publication number
WO2006136824A3
WO2006136824A3 PCT/GB2006/002279 GB2006002279W WO2006136824A3 WO 2006136824 A3 WO2006136824 A3 WO 2006136824A3 GB 2006002279 W GB2006002279 W GB 2006002279W WO 2006136824 A3 WO2006136824 A3 WO 2006136824A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence correlation
ratiometric
agents
production
methods
Prior art date
Application number
PCT/GB2006/002279
Other languages
French (fr)
Other versions
WO2006136824A2 (en
Inventor
Simon Webster
Original Assignee
Avacta Ltd
Simon Webster
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avacta Ltd, Simon Webster filed Critical Avacta Ltd
Publication of WO2006136824A2 publication Critical patent/WO2006136824A2/en
Publication of WO2006136824A3 publication Critical patent/WO2006136824A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/5432—Liposomes or microcapsules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/586—Liposomes, microcapsules or cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The present invention provides ratio-metrically labelled lipid carrier particles having at least two fluorescent signals and mixtures thereof, each carrier particle having incorporated in or on its shell surface at least one target receptor. The invention also includes a method of screening the particles with a range of probe ligands in sorting or binding assays and in a ratio-metric multicolour fluorescence correlation spectroscopy screen. The carrier particles are especially useful for carrying membrane proteins as the target receptors.
PCT/GB2006/002279 2005-06-23 2006-06-22 Ratiometric multicolour fluorescence correlation screening; agents for use therewith and methods of production thereof WO2006136824A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0512769A GB0512769D0 (en) 2005-06-23 2005-06-23 Ratiometic multicolour fluorescence correlation screening; agents for use therewith and methods of production thereof
GB0512769.1 2005-06-23

Publications (2)

Publication Number Publication Date
WO2006136824A2 WO2006136824A2 (en) 2006-12-28
WO2006136824A3 true WO2006136824A3 (en) 2007-03-29

Family

ID=34855998

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2006/002279 WO2006136824A2 (en) 2005-06-23 2006-06-22 Ratiometric multicolour fluorescence correlation screening; agents for use therewith and methods of production thereof

Country Status (2)

Country Link
GB (2) GB0512769D0 (en)
WO (1) WO2006136824A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110360925B (en) * 2018-04-10 2020-10-27 中国科学院物理研究所 Calibration method and calibration device for magnetic sphere coordinates of magnetic tweezers device
CN113125711B (en) * 2019-12-31 2023-07-28 科美博阳诊断技术(上海)有限公司 Acceptor reagent and application thereof
EP4310478A1 (en) * 2021-03-16 2024-01-24 Sony Group Corporation Closed-system automatic sample sorting system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002334A1 (en) * 1988-08-31 1990-03-08 Zynaxis Technologies, Inc. Reagents and methods for determination of analytes
EP0577092A2 (en) * 1992-07-02 1994-01-05 Becton, Dickinson and Company Immunoassays using microparticles containing different detectable substances
US5494803A (en) * 1991-11-19 1996-02-27 North Carolina State University Immunodiagnostic assay using liposomes carrying labels thereof on outer liposome surface
US20040033624A1 (en) * 2002-06-17 2004-02-19 Zweig Stephen Eliot Membrane receptor reagent and assay

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4193983A (en) * 1978-05-16 1980-03-18 Syva Company Labeled liposome particle compositions and immunoassays therewith
US4708933A (en) * 1984-06-12 1987-11-24 Leaf Huang Immunoliposome assay-methods and products
US7348181B2 (en) * 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
EP1125132A1 (en) * 1998-10-30 2001-08-22 Merska B.V. Screening for analytes using labeled receptors
EP1180242A1 (en) * 1999-05-27 2002-02-20 Zeptosens AG Vesicle containing polymers and sensor detection methods based thereon
WO2004101708A1 (en) * 2003-05-09 2004-11-25 Applera Corporation Fluorescent polymeric materials containing lipid soluble rhodamine dyes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002334A1 (en) * 1988-08-31 1990-03-08 Zynaxis Technologies, Inc. Reagents and methods for determination of analytes
US5494803A (en) * 1991-11-19 1996-02-27 North Carolina State University Immunodiagnostic assay using liposomes carrying labels thereof on outer liposome surface
EP0577092A2 (en) * 1992-07-02 1994-01-05 Becton, Dickinson and Company Immunoassays using microparticles containing different detectable substances
US20040033624A1 (en) * 2002-06-17 2004-02-19 Zweig Stephen Eliot Membrane receptor reagent and assay

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CARMONA-RIBEIRO, A.M.: "Bilayer vesicles and liposome as interface agents", CHEMICAL SOCIETY REVIEWS, vol. 30, 2001, pages 241 - 247, XP002402464 *
CHEN, I.A., ROBERTS, R.W. AND SZOSTAK, J.W.: "Matreials and Methods", 2004, XP002402466, Retrieved from the Internet <URL:http://genetics.mgh.harvard.edu/szostakweb/publications/Szostak_pdfs/Chen_et_al_2004_SI.pdf#search=%22bodipy%20%22500%2F510%22%20%22558%2F568%22%20liposome%22> *
FULTON R J ET AL: "Advanced multiplexd analysis with the FlowMetrix(TM) system", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, US, vol. 43, no. 9, September 1997 (1997-09-01), pages 1749 - 1756, XP002142645, ISSN: 0009-9147 *
RIGAUD J-L ET AL: "RECONSTITUTION OF MEMBRANE PROTEINS INTO LIPOSOMES", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 372, 2003, pages 65 - 86, XP009072936, ISSN: 0076-6879 *
SICCHIEROLLI, S.M. AND CARMONA-RIBEIRO, A.M.: "Incorporation of the cholera toxin receptor in phospholipid-covered polystyrene microspheres", COLLOIDS AND SURFACES B: BIOINTERFACES, vol. 5, 1995, pages 57 - 61, XP002402465 *
TRUNEH, AL., ET AL: "Antibody-bearing liposomes as multicolour immunofluorescence markers for flow cytometry and imaging", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 100, 1987, pages 59 - 71, XP002402463 *

Also Published As

Publication number Publication date
WO2006136824A2 (en) 2006-12-28
GB0512769D0 (en) 2005-07-27
GB2427689A (en) 2007-01-03
GB0612371D0 (en) 2006-08-02

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