ES2181821T3 - Procedimiento y composicion de reactivos mejorados para el recuento diferencial de leucocitos en muestras de sangre entera fresca y antigua en base a la actividad intrinseca de peroxidasa en los leucocitos. - Google Patents

Procedimiento y composicion de reactivos mejorados para el recuento diferencial de leucocitos en muestras de sangre entera fresca y antigua en base a la actividad intrinseca de peroxidasa en los leucocitos.

Info

Publication number
ES2181821T3
ES2181821T3 ES96106957T ES96106957T ES2181821T3 ES 2181821 T3 ES2181821 T3 ES 2181821T3 ES 96106957 T ES96106957 T ES 96106957T ES 96106957 T ES96106957 T ES 96106957T ES 2181821 T3 ES2181821 T3 ES 2181821T3
Authority
ES
Spain
Prior art keywords
samples
composition
peroxidase
analysis
differential
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96106957T
Other languages
English (en)
Inventor
Michael J Malin
Phyllis Shapiro
John F Cremins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Bayer Corp
Original Assignee
Bayer AG
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG, Bayer Corp filed Critical Bayer AG
Application granted granted Critical
Publication of ES2181821T3 publication Critical patent/ES2181821T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/106664—Blood serum or blood plasma standard or control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Ecology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION PROPORCIONA UNA COMPOSICION Y METODO MEJORADO PARA LLEVAR A CABO COMPUTOS DIFERENCIALES DE GLOBULOS BLANCOS Y ANALISIS DE SUBPOBLACION UTILIZANDO TANTO MUESTRAS DE SANGRE FRESCA COMO ANTIGUA CON SEGURIDAD Y PRECISION. LA INVENCION ES PARTICULARMENTE APLICABLE PARA EL ANALISIS DE MUESTRAS DE SANGRE ANTIGUAS QUE HAYAN SIDO ALMACENADAS A TEMPERATURA AMBIENTE DURANTE MAS DE UN DIA, PERMITIENDO ASI INFORMACION SEGURA Y UTIL A OBTENER DE MUESTRAS QUE NORMALMENTE SON CONSIDERADAS SUBOPTIMAS. LA COMPOSICION REACTIVA MEJORADA Y EL METODO ESTAN PARTICULARMENTE RELACIONADOS CON EL METODO DE PEROXIDASA DE DETERMINACIONES DIFERENCIALES DE GLOBULOS BLANCOS. UN ASPECTO DE LA INVENCION INCLUYE UNA COMPOSICION REACTIVA ACUOSA MEJORADA PARA LLEVAR A CABO EL METODO DE PEROXIDASA DE COMPUTO DIFERENCIAL. OTRO ASPECTO INCLUYE EL USO DE UN CICLO DE ENJUAGUE Y UNA SOLUCION DE ENJUAGUE LIBRE DE SURFACTANTE HEMOLITICO PARA ALIVIAR LOS EFECTOS ADVERSOS DE LA REALIZACION DEL ENJUAGUE Y PARA MODERNIZAR Y ECONOMIZAR EL PROCESO ANALITICO, PARTICULARMENTE CUANDO LOS ANALISIS SE REALIZAN EN ANALIZADORES DE HEMATOLOGIA AUTOMATICOS Y SISTEMAS DE CITOMETRIA DE FLUJO. LA COMPOSICION Y METODO DE LA INVENCION PROPORCIONAN DATOS CLINICAMENTE UTILES PARA EL ANALISIS DIFERENCIAL DE MUESTRAS DE SANGRE COMPLETAS.
ES96106957T 1995-05-16 1996-05-03 Procedimiento y composicion de reactivos mejorados para el recuento diferencial de leucocitos en muestras de sangre entera fresca y antigua en base a la actividad intrinseca de peroxidasa en los leucocitos. Expired - Lifetime ES2181821T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/442,491 US5639630A (en) 1995-05-16 1995-05-16 Method and reagent composition for performing leukocyte differential counts on fresh and aged whole blood samples, based on intrinsic peroxidase activity of leukocytes

Publications (1)

Publication Number Publication Date
ES2181821T3 true ES2181821T3 (es) 2003-03-01

Family

ID=23756987

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96106957T Expired - Lifetime ES2181821T3 (es) 1995-05-16 1996-05-03 Procedimiento y composicion de reactivos mejorados para el recuento diferencial de leucocitos en muestras de sangre entera fresca y antigua en base a la actividad intrinseca de peroxidasa en los leucocitos.

Country Status (12)

Country Link
US (1) US5639630A (es)
EP (1) EP0743519B1 (es)
JP (1) JP4361142B2 (es)
KR (1) KR960042055A (es)
AT (1) ATE223043T1 (es)
AU (1) AU699086C (es)
CA (1) CA2173519A1 (es)
DE (1) DE69623174T2 (es)
ES (1) ES2181821T3 (es)
IL (1) IL117862A (es)
PT (1) PT743519E (es)
TW (1) TW448295B (es)

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CN101349644B (zh) * 2007-07-20 2012-06-27 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法
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US8102161B2 (en) * 2007-09-25 2012-01-24 Tdk Corporation Stable output in a switching power supply by smoothing the output of the secondary coil
CN101475754A (zh) * 2008-01-04 2009-07-08 深圳迈瑞生物医疗电子股份有限公司 不对称菁类荧光染料,组合物及在生物样品染色中的用途
CN101602762B (zh) * 2008-06-10 2013-10-16 深圳迈瑞生物医疗电子股份有限公司 不对称菁类化合物、其制备方法及应用
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CN104520418A (zh) * 2012-05-16 2015-04-15 加利福尼亚大学董事会 用于溶解细胞和/或组织的组合物
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JP7143678B2 (ja) * 2018-08-24 2022-09-29 東ソー株式会社 標的細胞を検出する方法
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Also Published As

Publication number Publication date
CA2173519A1 (en) 1996-11-17
EP0743519A2 (en) 1996-11-20
EP0743519A3 (en) 1998-07-29
DE69623174T2 (de) 2002-12-19
JP4361142B2 (ja) 2009-11-11
JPH08308593A (ja) 1996-11-26
KR960042055A (ko) 1996-12-19
DE69623174D1 (de) 2002-10-02
EP0743519B1 (en) 2002-08-28
US5639630A (en) 1997-06-17
IL117862A0 (en) 1996-08-04
IL117862A (en) 1999-12-22
AU5226296A (en) 1996-11-28
ATE223043T1 (de) 2002-09-15
TW448295B (en) 2001-08-01
PT743519E (pt) 2002-12-31
AU699086C (en) 2001-06-21
AU699086B2 (en) 1998-11-19

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