ES2131069T3 - Expresion de proteinas recombinantes en bacterias atenuadas. - Google Patents

Expresion de proteinas recombinantes en bacterias atenuadas.

Info

Publication number
ES2131069T3
ES2131069T3 ES92905914T ES92905914T ES2131069T3 ES 2131069 T3 ES2131069 T3 ES 2131069T3 ES 92905914 T ES92905914 T ES 92905914T ES 92905914 T ES92905914 T ES 92905914T ES 2131069 T3 ES2131069 T3 ES 2131069T3
Authority
ES
Spain
Prior art keywords
expression
attenuated bacteria
recombinant proteins
promoter
attenuated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92905914T
Other languages
English (en)
Inventor
Ian George Charles
Steven Neville Chatfield
Neil Fraser Fairweather
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wellcome Foundation Ltd
Original Assignee
Wellcome Foundation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB919104596A external-priority patent/GB9104596D0/en
Priority claimed from GB919121208A external-priority patent/GB9121208D0/en
Application filed by Wellcome Foundation Ltd filed Critical Wellcome Foundation Ltd
Application granted granted Critical
Publication of ES2131069T3 publication Critical patent/ES2131069T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/025—Enterobacteriales, e.g. Enterobacter
    • A61K39/0275—Salmonella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

UNA BACTERIA ATENUADA LA CUAL ES CAPAZ DE EXPRESAR UNA PROTEINA HETEROLOGA, SIENDO BAJO EL CONTROL DE UN PROMOTOR CUYA ACTIVIDAD ES INDUCIDA POR CONDICIONES ANAEROBICAS, SE PUEDE USAR COMO UNA VACUNA. UN PROMOTOR ADECUADO ES EL PROMOTOR "NIRB".
ES92905914T 1991-03-05 1992-03-05 Expresion de proteinas recombinantes en bacterias atenuadas. Expired - Lifetime ES2131069T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB919104596A GB9104596D0 (en) 1991-03-05 1991-03-05 Production of recombinant proteins
GB919121208A GB9121208D0 (en) 1991-10-04 1991-10-04 Vaccines

Publications (1)

Publication Number Publication Date
ES2131069T3 true ES2131069T3 (es) 1999-07-16

Family

ID=26298523

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92905914T Expired - Lifetime ES2131069T3 (es) 1991-03-05 1992-03-05 Expresion de proteinas recombinantes en bacterias atenuadas.

Country Status (18)

Country Link
US (2) US5547664A (es)
EP (1) EP0574466B1 (es)
JP (1) JP3415145B2 (es)
KR (1) KR100240182B1 (es)
AT (1) ATE180280T1 (es)
AU (1) AU664360B2 (es)
CA (1) CA2099841C (es)
CZ (1) CZ285118B6 (es)
DE (1) DE69229221T2 (es)
DK (1) DK0574466T3 (es)
ES (1) ES2131069T3 (es)
FI (1) FI110008B (es)
GR (1) GR3030778T3 (es)
HU (1) HU219535B (es)
NO (1) NO309331B1 (es)
PL (1) PL170938B1 (es)
SK (1) SK55593A3 (es)
WO (1) WO1992015689A1 (es)

Families Citing this family (48)

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GB9209118D0 (en) * 1992-04-28 1992-06-10 Sb 120 Amsterdam Bv Vaccine compositions
DE69322645T2 (de) 1992-07-31 1999-05-20 Medeva Holdings B.V., Amsterdam Expression rekombinanter fusionsproteine in attenuierten bakterien
JPH09500540A (ja) * 1993-07-30 1997-01-21 メデヴァ ホールディングス ビー．ヴイ． ワクチン組成物
GB9401787D0 (en) 1994-01-31 1994-03-23 Medeva Holdings Bv Vaccine compositions
GB9401795D0 (en) * 1994-01-31 1994-03-23 Medeva Holdings Bv Vaccines
GB2295394B (en) * 1994-01-31 1997-09-24 Medeva Holdings Bv DNA encoding a fusion protein comprising the C fragment of tetanus toxin
AUPM612494A0 (en) * 1994-06-08 1994-06-30 Csl Limited Treatment or prevention of helicobacter infection
US6254874B1 (en) * 1995-04-13 2001-07-03 President And Fellows Of Harvard College Attenuated auxotrophic microorganisms having a combination of non-attenuating mutations and method for making same
US6190657B1 (en) 1995-06-07 2001-02-20 Yale University Vectors for the diagnosis and treatment of solid tumors including melanoma
GB9521568D0 (en) * 1995-10-20 1995-12-20 Lynxvale Ltd Delivery of biologically active polypeptides
US20030125278A1 (en) * 1997-08-13 2003-07-03 Tang De-Chu C. Immunization of animals by topical applications of a salmonella-based vector
US6080849A (en) 1997-09-10 2000-06-27 Vion Pharmaceuticals, Inc. Genetically modified tumor-targeted bacteria with reduced virulence
JP2002500001A (ja) 1997-09-10 2002-01-08 ヴァイオン ファーマシューティカルズ、インコーポレーテッド 低下した毒性を有する遺伝子改変腫瘍標的化細菌
US6905691B1 (en) 1997-12-11 2005-06-14 Celltech Pharma Europe Limited Vaccines containing attenuated bacteria
GB9806449D0 (en) 1998-03-25 1998-05-27 Peptide Therapeutics Ltd Attenuated bacteria useful in vaccines
US6585975B1 (en) * 1998-04-30 2003-07-01 Acambis, Inc. Use of Salmonella vectors for vaccination against helicobacter infection
FR2778922A1 (fr) * 1998-05-19 1999-11-26 Pasteur Institut Polynucleotides contenant des sequences de regulation de la synthese de toxines de clostridium, et microorganismes les comprenant
US6413768B1 (en) * 1998-12-02 2002-07-02 University Of Maryland Expression plasmids
US6790950B2 (en) * 1999-04-09 2004-09-14 Pharmacia & Upjohn Company Anti-bacterial vaccine compositions
EP2281834B1 (en) 1999-04-09 2015-03-04 Zoetis P&U LLC Attenuated Pasteurellaceae bacterium having a mutation in a virulence gene
US20040009936A1 (en) * 1999-05-03 2004-01-15 Tang De-Chu C. Vaccine and drug delivery by topical application of vectors and vector extracts
US6962696B1 (en) 1999-10-04 2005-11-08 Vion Pharmaceuticals Inc. Compositions and methods for tumor-targeted delivery of effector molecules
GB0024203D0 (en) * 2000-10-03 2000-11-15 Peptide Therapeutics Ltd Stabilisation of plasmid inheritance in bacteria
EP1343870A4 (en) * 2000-11-09 2005-07-13 Univ Queensland BACTERIAL EXPRESSION
US7780961B2 (en) * 2001-05-03 2010-08-24 Actogenix N.V. Self-containing Lactococcus strain
GB0121998D0 (en) 2001-09-11 2001-10-31 Acambis Res Ltd Attenuated bacteria useful in vaccines
AUPR977601A0 (en) * 2001-12-28 2002-01-31 Delta Biotechnology Limited Defective entities and uses therefor
GB0205376D0 (en) * 2002-03-07 2002-04-24 Royal Holloway University Of L Spore germination
EP1513545B1 (en) * 2002-06-19 2008-03-19 Actogenix N.V. Methods and means to promote gut absorption
CA2506031A1 (en) * 2002-11-15 2004-06-03 Vib Vzw Self-containing lactobacillus strain comprising a thya mutation and therapeutic applications thereof
EP1789094B1 (en) * 2004-08-13 2014-12-10 MARSHALL, Barry J. Bacterial delivery system
US8029777B2 (en) 2004-08-13 2011-10-04 Marshall Barry J Helicobacter system and uses thereof
EP2119450B1 (en) 2005-11-29 2013-02-13 Actogenix N.V. Induction of mucosal tolerance to pancreatic islet beta-cell autoantigens
MY183797A (en) 2006-09-18 2021-03-16 Univ Arkansas Compositions and methods of enhancing immune responses
EP2125010B1 (en) 2007-01-25 2014-06-04 Actogenix N.V. Treatment of immune disease by mucosal delivery of antigens using genetically modified lactobacillus
US9125854B2 (en) * 2007-10-30 2015-09-08 The Board Of Trustees Of The University Of Arkansas Compositions and methods of enhancing immune responses to flagellated bacterium
BRPI0819229B1 (pt) 2007-11-01 2019-01-22 The Board Of Trustees Of The University Of Arkansas vacinas para reforçar reações imunes contra eimeria
NZ601609A (en) 2010-01-21 2014-08-29 Univ Arkansas Vaccine vectors and methods of enhancing immune responses
US9597379B1 (en) 2010-02-09 2017-03-21 David Gordon Bermudes Protease inhibitor combination with therapeutic proteins including antibodies
JP5746333B2 (ja) 2010-06-09 2015-07-08 ザ ボード オブ トラスティーズ オブ ザ ユニバーシティ オブ アーカンソー カンピロバクター感染を減少させるワクチン及び方法
CN102477440A (zh) * 2010-11-29 2012-05-30 南京大学 治疗基因在厌氧组织靶向递送和选择性稳定表达方法及应用
EP3763385A1 (en) 2011-03-21 2021-01-13 Altimmune Inc. Rapid and prolonged immunologic-therapeutic
US10183069B2 (en) 2011-03-21 2019-01-22 Altimmune Inc. Rapid and prolonged immunologic-therapeutic
WO2014127185A1 (en) 2013-02-14 2014-08-21 The Board Of Trustees Of The University Of Arkansas Compositions and methods of enhancing immune responses to eimeria or limiting eimeria infection
MY173901A (en) 2013-03-15 2020-02-26 Texas A & M Univ Sys Compositions and methods of enhancing immune responses to enteric pathogens
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
AU2017260323B2 (en) 2016-05-03 2023-11-16 The Board Of Trustees Of The University Of Arkansas Yeast vaccine vector including immunostimulatory and antigenic polypeptides and methods of using the same
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria

Family Cites Families (8)

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US4837151A (en) * 1980-05-19 1989-06-06 The Board Of Trustees Of The Leland Stanford Junior University, Stanford University Live vaccines comprising two mutations and foreign antigen
DE3735381A1 (de) * 1987-03-31 1989-05-03 Boehringer Mannheim Gmbh Rekombinante dna zur reprimierbaren und induzierbaren expression von fremdgenen
GB8730037D0 (en) * 1987-12-23 1988-02-03 Wellcome Found Vaccines
JPH0284172A (ja) * 1988-07-21 1990-03-26 Smithkline Beckman Corp 異種遺伝子の発現能を有し、組換え型ワクチンとして有用なサルモネラ形質転換体
DE3926076A1 (de) * 1989-04-21 1990-10-25 Boehringer Mannheim Gmbh Rekombinante dna und expressionsvektor
GB8912330D0 (en) * 1989-05-30 1989-07-12 Wellcome Found Live vaccines
GB9007194D0 (en) * 1990-03-30 1990-05-30 Wellcome Found Live vaccines
GB9104596D0 (en) * 1991-03-05 1991-04-17 Wellcome Found Production of recombinant proteins

Also Published As

Publication number Publication date
CZ100593A3 (en) 1994-01-19
AU1350892A (en) 1992-10-06
WO1992015689A1 (en) 1992-09-17
PL296702A1 (en) 1993-07-26
AU664360B2 (en) 1995-11-16
NO309331B1 (no) 2001-01-15
ATE180280T1 (de) 1999-06-15
SK55593A3 (en) 1993-10-06
CA2099841C (en) 2003-02-25
FI110008B (fi) 2002-11-15
EP0574466B1 (en) 1999-05-19
HUT66833A (en) 1995-01-30
FI933757A0 (fi) 1993-08-26
PL170938B1 (pl) 1997-02-28
FI933757L (fi) 1993-08-26
CZ285118B6 (cs) 1999-05-12
CA2099841A1 (en) 1992-09-06
NO932423D0 (no) 1993-07-02
HU9302492D0 (en) 1993-11-29
NO932423L (no) 1993-07-02
EP0574466A1 (en) 1993-12-22
US5547664A (en) 1996-08-20
GR3030778T3 (en) 1999-11-30
JPH06505158A (ja) 1994-06-16
DE69229221D1 (de) 1999-06-24
US5683700A (en) 1997-11-04
HU219535B (hu) 2001-05-28
JP3415145B2 (ja) 2003-06-09
KR100240182B1 (ko) 2000-01-15
DE69229221T2 (de) 1999-09-23
DK0574466T3 (da) 1999-11-08

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