ES2131069T3 - Expresion de proteinas recombinantes en bacterias atenuadas. - Google Patents
Expresion de proteinas recombinantes en bacterias atenuadas.Info
- Publication number
- ES2131069T3 ES2131069T3 ES92905914T ES92905914T ES2131069T3 ES 2131069 T3 ES2131069 T3 ES 2131069T3 ES 92905914 T ES92905914 T ES 92905914T ES 92905914 T ES92905914 T ES 92905914T ES 2131069 T3 ES2131069 T3 ES 2131069T3
- Authority
- ES
- Spain
- Prior art keywords
- expression
- attenuated bacteria
- recombinant proteins
- promoter
- attenuated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000894006 Bacteria Species 0.000 title abstract 2
- 230000002238 attenuated effect Effects 0.000 title abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 230000000694 effects Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/025—Enterobacteriales, e.g. Enterobacter
- A61K39/0275—Salmonella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
- A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
- A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
UNA BACTERIA ATENUADA LA CUAL ES CAPAZ DE EXPRESAR UNA PROTEINA HETEROLOGA, SIENDO BAJO EL CONTROL DE UN PROMOTOR CUYA ACTIVIDAD ES INDUCIDA POR CONDICIONES ANAEROBICAS, SE PUEDE USAR COMO UNA VACUNA. UN PROMOTOR ADECUADO ES EL PROMOTOR "NIRB".
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB919104596A GB9104596D0 (en) | 1991-03-05 | 1991-03-05 | Production of recombinant proteins |
GB919121208A GB9121208D0 (en) | 1991-10-04 | 1991-10-04 | Vaccines |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2131069T3 true ES2131069T3 (es) | 1999-07-16 |
Family
ID=26298523
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES92905914T Expired - Lifetime ES2131069T3 (es) | 1991-03-05 | 1992-03-05 | Expresion de proteinas recombinantes en bacterias atenuadas. |
Country Status (18)
Country | Link |
---|---|
US (2) | US5547664A (es) |
EP (1) | EP0574466B1 (es) |
JP (1) | JP3415145B2 (es) |
KR (1) | KR100240182B1 (es) |
AT (1) | ATE180280T1 (es) |
AU (1) | AU664360B2 (es) |
CA (1) | CA2099841C (es) |
CZ (1) | CZ285118B6 (es) |
DE (1) | DE69229221T2 (es) |
DK (1) | DK0574466T3 (es) |
ES (1) | ES2131069T3 (es) |
FI (1) | FI110008B (es) |
GR (1) | GR3030778T3 (es) |
HU (1) | HU219535B (es) |
NO (1) | NO309331B1 (es) |
PL (1) | PL170938B1 (es) |
SK (1) | SK55593A3 (es) |
WO (1) | WO1992015689A1 (es) |
Families Citing this family (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9209118D0 (en) * | 1992-04-28 | 1992-06-10 | Sb 120 Amsterdam Bv | Vaccine compositions |
DK0652962T3 (da) | 1992-07-31 | 1999-08-23 | Medeva Holdings Bv | Ekspression af rekombinante fusionsproteiner i attenuerede bakterier |
GB9401787D0 (en) | 1994-01-31 | 1994-03-23 | Medeva Holdings Bv | Vaccine compositions |
EP0712442B1 (en) * | 1993-07-30 | 2002-03-27 | Medeva Holdings B.V. | Vaccine compositions |
GB9401795D0 (en) * | 1994-01-31 | 1994-03-23 | Medeva Holdings Bv | Vaccines |
GB2295394B (en) * | 1994-01-31 | 1997-09-24 | Medeva Holdings Bv | DNA encoding a fusion protein comprising the C fragment of tetanus toxin |
AUPM612494A0 (en) * | 1994-06-08 | 1994-06-30 | Csl Limited | Treatment or prevention of helicobacter infection |
US6254874B1 (en) * | 1995-04-13 | 2001-07-03 | President And Fellows Of Harvard College | Attenuated auxotrophic microorganisms having a combination of non-attenuating mutations and method for making same |
US6190657B1 (en) | 1995-06-07 | 2001-02-20 | Yale University | Vectors for the diagnosis and treatment of solid tumors including melanoma |
GB9521568D0 (en) * | 1995-10-20 | 1995-12-20 | Lynxvale Ltd | Delivery of biologically active polypeptides |
US20030125278A1 (en) * | 1997-08-13 | 2003-07-03 | Tang De-Chu C. | Immunization of animals by topical applications of a salmonella-based vector |
CN1253551C (zh) | 1997-09-10 | 2006-04-26 | 维昂药品公司 | 减毒的遗传修饰肿瘤靶向细菌 |
US6080849A (en) | 1997-09-10 | 2000-06-27 | Vion Pharmaceuticals, Inc. | Genetically modified tumor-targeted bacteria with reduced virulence |
US6905691B1 (en) | 1997-12-11 | 2005-06-14 | Celltech Pharma Europe Limited | Vaccines containing attenuated bacteria |
GB9806449D0 (en) | 1998-03-25 | 1998-05-27 | Peptide Therapeutics Ltd | Attenuated bacteria useful in vaccines |
US6585975B1 (en) * | 1998-04-30 | 2003-07-01 | Acambis, Inc. | Use of Salmonella vectors for vaccination against helicobacter infection |
FR2778922A1 (fr) * | 1998-05-19 | 1999-11-26 | Pasteur Institut | Polynucleotides contenant des sequences de regulation de la synthese de toxines de clostridium, et microorganismes les comprenant |
US6413768B1 (en) * | 1998-12-02 | 2002-07-02 | University Of Maryland | Expression plasmids |
EP1171577A2 (en) * | 1999-04-09 | 2002-01-16 | PHARMACIA & UPJOHN COMPANY | Anti-bacterial vaccine compositions |
US6790950B2 (en) * | 1999-04-09 | 2004-09-14 | Pharmacia & Upjohn Company | Anti-bacterial vaccine compositions |
US20040009936A1 (en) * | 1999-05-03 | 2004-01-15 | Tang De-Chu C. | Vaccine and drug delivery by topical application of vectors and vector extracts |
US6962696B1 (en) | 1999-10-04 | 2005-11-08 | Vion Pharmaceuticals Inc. | Compositions and methods for tumor-targeted delivery of effector molecules |
GB0024203D0 (en) * | 2000-10-03 | 2000-11-15 | Peptide Therapeutics Ltd | Stabilisation of plasmid inheritance in bacteria |
EP1343870A4 (en) * | 2000-11-09 | 2005-07-13 | Univ Queensland | BACTERIAL EXPRESSION SYSTEMS |
US7780961B2 (en) * | 2001-05-03 | 2010-08-24 | Actogenix N.V. | Self-containing Lactococcus strain |
GB0121998D0 (en) | 2001-09-11 | 2001-10-31 | Acambis Res Ltd | Attenuated bacteria useful in vaccines |
AUPR977601A0 (en) * | 2001-12-28 | 2002-01-31 | Delta Biotechnology Limited | Defective entities and uses therefor |
GB0205376D0 (en) * | 2002-03-07 | 2002-04-24 | Royal Holloway University Of L | Spore germination |
EP1513545B1 (en) * | 2002-06-19 | 2008-03-19 | Actogenix N.V. | Methods and means to promote gut absorption |
WO2004046346A2 (en) * | 2002-11-15 | 2004-06-03 | Vib Vzw | Self-containing lactobacillus strain |
US8029777B2 (en) | 2004-08-13 | 2011-10-04 | Marshall Barry J | Helicobacter system and uses thereof |
JP2008509168A (ja) | 2004-08-13 | 2008-03-27 | マーシャル,バリー,ジェー. | 細菌デリバリシステム |
CA2631598C (en) | 2005-11-29 | 2017-06-27 | Actogenix Nv | Induction of mucosal tolerance to antigens |
PL2066339T3 (pl) * | 2006-09-18 | 2015-02-27 | Univ Arkansas | Kompozycje i sposoby zwiększania odpowiedzi immunologicznych |
JP2010516269A (ja) | 2007-01-25 | 2010-05-20 | アクトジェニックス・エヌブイ | 抗原の粘膜送達による免疫疾患の治療 |
US9125854B2 (en) * | 2007-10-30 | 2015-09-08 | The Board Of Trustees Of The University Of Arkansas | Compositions and methods of enhancing immune responses to flagellated bacterium |
DK3097926T3 (da) * | 2007-11-01 | 2019-12-16 | Univ Arkansas | Sammensætninger og fremgangsmåder til forstærkning af immunreaktioner på Eimeria |
DK2525817T3 (en) | 2010-01-21 | 2017-10-02 | Univ Arkansas | Vaccine vectors and methods for enhancing immune responses |
US9597379B1 (en) | 2010-02-09 | 2017-03-21 | David Gordon Bermudes | Protease inhibitor combination with therapeutic proteins including antibodies |
NZ702839A (en) | 2010-06-09 | 2016-04-29 | Univ Arkansas | Vaccine and methods to reduce campylobacter infection |
CN102477440A (zh) * | 2010-11-29 | 2012-05-30 | 南京大学 | 治疗基因在厌氧组织靶向递送和选择性稳定表达方法及应用 |
KR20200008014A (ko) | 2011-03-21 | 2020-01-22 | 알티뮨 인크. | 급속 및 지속적 면역학적제제-치료제 |
US10183069B2 (en) | 2011-03-21 | 2019-01-22 | Altimmune Inc. | Rapid and prolonged immunologic-therapeutic |
PT2956165T (pt) | 2013-02-14 | 2019-11-29 | Texas A & M Univ Sys | Composições e métodos de potenciação das respostas imunitárias frente a eimeria ou limitação de uma infeção por eimeria |
AU2014239557B2 (en) | 2013-03-15 | 2019-01-03 | The Board Of Trustees Of The University Of Arkansas | Compositions and methods of enhancing immune responses to enteric pathogens |
JP7467027B2 (ja) | 2016-05-03 | 2024-04-15 | ザ ボード オブ トラスティーズ オブ ザ ユニバーシティ オブ アーカンソー | 免疫刺激性ポリペプチドおよび抗原性ポリペプチドを含む酵母ワクチンベクター並びにそれを使用する方法 |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4837151A (en) * | 1980-05-19 | 1989-06-06 | The Board Of Trustees Of The Leland Stanford Junior University, Stanford University | Live vaccines comprising two mutations and foreign antigen |
DE3735381A1 (de) * | 1987-03-31 | 1989-05-03 | Boehringer Mannheim Gmbh | Rekombinante dna zur reprimierbaren und induzierbaren expression von fremdgenen |
GB8730037D0 (en) * | 1987-12-23 | 1988-02-03 | Wellcome Found | Vaccines |
JPH0284172A (ja) * | 1988-07-21 | 1990-03-26 | Smithkline Beckman Corp | 異種遺伝子の発現能を有し、組換え型ワクチンとして有用なサルモネラ形質転換体 |
DE3926076A1 (de) * | 1989-04-21 | 1990-10-25 | Boehringer Mannheim Gmbh | Rekombinante dna und expressionsvektor |
GB8912330D0 (en) * | 1989-05-30 | 1989-07-12 | Wellcome Found | Live vaccines |
GB9007194D0 (en) * | 1990-03-30 | 1990-05-30 | Wellcome Found | Live vaccines |
GB9104596D0 (en) * | 1991-03-05 | 1991-04-17 | Wellcome Found | Production of recombinant proteins |
-
1992
- 1992-03-05 CA CA002099841A patent/CA2099841C/en not_active Expired - Lifetime
- 1992-03-05 EP EP92905914A patent/EP0574466B1/en not_active Expired - Lifetime
- 1992-03-05 AT AT92905914T patent/ATE180280T1/de active
- 1992-03-05 ES ES92905914T patent/ES2131069T3/es not_active Expired - Lifetime
- 1992-03-05 PL PL92296702A patent/PL170938B1/pl unknown
- 1992-03-05 KR KR1019930702594A patent/KR100240182B1/ko not_active IP Right Cessation
- 1992-03-05 SK SK55593A patent/SK55593A3/sk unknown
- 1992-03-05 JP JP50556392A patent/JP3415145B2/ja not_active Expired - Lifetime
- 1992-03-05 CZ CZ931005A patent/CZ285118B6/cs not_active IP Right Cessation
- 1992-03-05 DE DE69229221T patent/DE69229221T2/de not_active Expired - Lifetime
- 1992-03-05 HU HU9302492A patent/HU219535B/hu unknown
- 1992-03-05 WO PCT/GB1992/000387 patent/WO1992015689A1/en active IP Right Grant
- 1992-03-05 AU AU13508/92A patent/AU664360B2/en not_active Expired
- 1992-03-05 DK DK92905914T patent/DK0574466T3/da active
-
1993
- 1993-07-02 NO NO932423A patent/NO309331B1/no not_active IP Right Cessation
- 1993-08-26 FI FI933757A patent/FI110008B/fi not_active IP Right Cessation
-
1994
- 1994-12-12 US US08/354,776 patent/US5547664A/en not_active Expired - Lifetime
-
1995
- 1995-06-06 US US08/469,507 patent/US5683700A/en not_active Expired - Lifetime
-
1999
- 1999-07-14 GR GR990401865T patent/GR3030778T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
NO309331B1 (no) | 2001-01-15 |
US5547664A (en) | 1996-08-20 |
PL170938B1 (pl) | 1997-02-28 |
WO1992015689A1 (en) | 1992-09-17 |
EP0574466A1 (en) | 1993-12-22 |
NO932423D0 (no) | 1993-07-02 |
JP3415145B2 (ja) | 2003-06-09 |
AU664360B2 (en) | 1995-11-16 |
CA2099841C (en) | 2003-02-25 |
PL296702A1 (en) | 1993-07-26 |
FI933757A (fi) | 1993-08-26 |
FI933757A0 (fi) | 1993-08-26 |
HU219535B (hu) | 2001-05-28 |
EP0574466B1 (en) | 1999-05-19 |
CZ285118B6 (cs) | 1999-05-12 |
SK55593A3 (en) | 1993-10-06 |
HU9302492D0 (en) | 1993-11-29 |
GR3030778T3 (en) | 1999-11-30 |
DE69229221T2 (de) | 1999-09-23 |
CA2099841A1 (en) | 1992-09-06 |
US5683700A (en) | 1997-11-04 |
AU1350892A (en) | 1992-10-06 |
HUT66833A (en) | 1995-01-30 |
DK0574466T3 (da) | 1999-11-08 |
DE69229221D1 (de) | 1999-06-24 |
FI110008B (fi) | 2002-11-15 |
CZ100593A3 (en) | 1994-01-19 |
KR100240182B1 (ko) | 2000-01-15 |
ATE180280T1 (de) | 1999-06-15 |
JPH06505158A (ja) | 1994-06-16 |
NO932423L (no) | 1993-07-02 |
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