ES2110446T3 - Metodo y kit para la separacion, concentracion y analisis de celulas. - Google Patents

Metodo y kit para la separacion, concentracion y analisis de celulas.

Info

Publication number
ES2110446T3
ES2110446T3 ES91913748T ES91913748T ES2110446T3 ES 2110446 T3 ES2110446 T3 ES 2110446T3 ES 91913748 T ES91913748 T ES 91913748T ES 91913748 T ES91913748 T ES 91913748T ES 2110446 T3 ES2110446 T3 ES 2110446T3
Authority
ES
Spain
Prior art keywords
cells
materials
milk
cultures
extracts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91913748T
Other languages
English (en)
Inventor
Edward E Pahuski
Randall L Dimond
John H Priest
Lisa S Martin
Kathleen K Stebnitz
Leopoldo G Mendoza
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ES2110446T3 publication Critical patent/ES2110446T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

SE PROPORCIONAN PROCESOS PARA ELIMINAR CELULAS COMO LAS PILDORAS CELULARES DE MUESTRAS DE LECHE LIQUIDA, O DE CULTIVOS O EXTRACTOS DE OTRAS MATERIAS COMESTIBLES U OTRAS MATERIAS DE ORIGEN BIOLOGICO. LAS CELULAS CONCENTRADAS EN LA PASTILLA PUEDEN SER ANALIZADAS POR VARIAS TECNICAS PARA DETERMINAR LA CUENTA CELULAR RELATIVA, INCLUYENDO LOS ANALISIS ESTANDAR DE BREED SMEAR PARA DETERMINAR DIRECTAMENTE LA CUENTA DE CELULAS O LISIS CELULAR SEGUIDA DE LA MEDICION DE ATP. LA AMPLIFICACION NUCLEICA, COMO POR EL METODO DE REACCION EN CADENA DE LA POLIMERASA, PUEDE SER LLEVADA A CABO USANDO LA PASTILLA CELULAR DIRECTAMENTE, SIN NECESIDAD DE AISLAMIENTO DEL ACIDO NUCLEICO DE LAS CELULAS. DESPUES DE LA AMPLIFICACION, SE PUEDE LLEVAR A CABO UN ENSAYO PARA UN SEGMENTO DEL ACIDO NUCLEICO AMPLIFICADO INDICATIVO DE LA PRESENCIA DE CELULAS DE INTERES EN LA MUESTRA. LA INVENCION PROPORCIONA TAMBIEN METODOS PARA OBTENER COMPUESTOS CELULARES DE LAS MUESTRAS DE LECHE, Y DE CULTIVOS O EXTRACTOS DE OTRAS MATERIAS,INCLUYENDO MATERIAS COMESTIBLES, Y PARA DETERMINAR LA CONTAMINACION RELATIVA DE LA LECHE ASI COMO DE OTROS MATERIALES POR MICROORGANISMOS. LA INVENCION TAMBIEN PROPORCIONA LOS INSTRUMENTOS PARA LLEVAR A CABO LOS VARIOS METODOS.
ES91913748T 1990-07-02 1991-07-02 Metodo y kit para la separacion, concentracion y analisis de celulas. Expired - Lifetime ES2110446T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US54798190A 1990-07-02 1990-07-02

Publications (1)

Publication Number Publication Date
ES2110446T3 true ES2110446T3 (es) 1998-02-16

Family

ID=24186943

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91913748T Expired - Lifetime ES2110446T3 (es) 1990-07-02 1991-07-02 Metodo y kit para la separacion, concentracion y analisis de celulas.

Country Status (8)

Country Link
US (2) US5587286A (es)
EP (1) EP0542790B1 (es)
JP (2) JPH04228097A (es)
AT (1) ATE161015T1 (es)
AU (1) AU8296691A (es)
DE (1) DE69128425T2 (es)
ES (1) ES2110446T3 (es)
WO (1) WO1992000317A1 (es)

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JPH04228097A (ja) * 1990-07-02 1992-08-18 Toyo Ink Mfg Co Ltd ミルク試料からの細胞分離および濃縮方法とそのキット
DE9304954U1 (de) * 1992-04-13 1993-08-12 Biolac GmbH Gesellschaft für industrielle Nutzung von Milchinhaltsstoffen, 31097 Harbarnsen Analyse-Kit zur Bestimmung der bakteriellen Keimzahl in Molke und Molkeninhaltsstoffen
US5646263A (en) * 1994-09-19 1997-07-08 Promega Corporation High efficiency method for isolating target substances using a multisample separation device
GB9522679D0 (en) * 1995-11-06 1996-01-10 Celsis Int Plc Assay for microrganisms and device for use therein
US5849488A (en) * 1996-02-27 1998-12-15 Oulutech Ltd. DNA-sequence-based diagnosis of mastitis from a milk sample
US5908751A (en) * 1996-04-26 1999-06-01 Toyo Ink Mfg. Co., Ltd. Method for detecting and/or determining ATP from microorganism cells in a sample
US5902722A (en) * 1997-12-05 1999-05-11 The Perkin-Elmer Corporation Method of detecting organisms in a sample
CA2347004A1 (en) * 1998-10-19 2000-04-27 Cbd Technologies Ltd. Methods of concentrating microorganisms using affinity separation
DE19904057C2 (de) * 1999-02-02 2003-01-30 Omya Ag Oftringen Verfahren zur Bestimmung der mikrobiellen Kontamination
BR7901407U (pt) * 1999-07-16 2001-02-28 Andre Fernando Alves De Olivei Tubo com escala especial destinado a teste para análise da quantidade de células
GB2352652A (en) * 1999-08-06 2001-02-07 Fsm Technologies Ltd Pre-treating hollow fibre membranes for micro-organism detection
MXPA03012075A (es) * 2001-06-22 2005-07-01 Marshfield Clinic Metodos y oligonucleotidos para la deteccion de salmonela sp., e. coli o157:h7 y listeria monocitogenes.
US7268229B2 (en) * 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
US7291326B2 (en) 2003-01-06 2007-11-06 Nutrition Physiology Corporation Compositions and methods for reducing the pathogen content of meat and meat products
US20040028665A1 (en) 2002-01-08 2004-02-12 Garner Bryan E. Compositions and methods for inhibiting pathogenic growth
US6709868B2 (en) * 2002-05-20 2004-03-23 Portascience Inc. Method and apparatus for measuring white blood cell count
KR100473703B1 (ko) * 2002-08-23 2005-03-10 한미약품 주식회사 형질전환 동물의 젖으로부터 재조합 펩타이드 또는단백질의 정제 방법
KR20040022903A (ko) * 2002-09-10 2004-03-18 에스케이케미칼주식회사 견섬유의 정련방법
EP2325328B1 (en) * 2002-12-23 2014-02-12 Promega Corporation Improved luciferase-based assays
US7132249B1 (en) 2003-05-12 2006-11-07 Charm Sciences, Inc. Method of determining allergenic food on surfaces
US7494781B1 (en) * 2003-05-12 2009-02-24 Charm Sciences, Inc. Sensitive method for detecting low levels of ATP
TW200506345A (en) * 2003-08-14 2005-02-16 Au Optronics Corp Water quality analysis method using potassium permanganate
WO2005022119A2 (en) * 2003-08-29 2005-03-10 Nutrition Physiology Corporation Methods for detecting and quantifying specific microorganisms
US20050048515A1 (en) * 2003-08-29 2005-03-03 Garner Bryan E. Methods for detecting and quantifying specific probiotic microorganisms in animal feed
WO2005028680A2 (en) * 2003-09-12 2005-03-31 Biocontrol Systems, Inc. Methods, compositions, and kits for the concentration and detection of microorganisms
JP4810871B2 (ja) * 2004-09-03 2011-11-09 パナソニック株式会社 微生物検出方法
US20060223071A1 (en) * 2005-04-01 2006-10-05 Wisniewski Michele E Methods, compositions, and kits for detecting nucleic acids in a single vessel
US20060240442A1 (en) * 2005-04-20 2006-10-26 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
US20060246463A1 (en) * 2005-04-20 2006-11-02 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
JP4685528B2 (ja) * 2005-07-07 2011-05-18 株式会社日清製粉グループ本社 微生物数迅速測定用食品試料の前処理方法
US20100129840A1 (en) * 2006-10-13 2010-05-27 Charm Scineces, Inc Method and Apparatus for Reducing Luminescent Test Result Interferences
EP2853587B1 (en) * 2009-06-18 2017-10-11 Merck Patent GmbH Method for isolating cells
DE102011077238B4 (de) * 2011-06-09 2016-03-31 Bayerische Motoren Werke Aktiengesellschaft Verfahren zum Nachweis von Mikroorganismen in einem Hohlraumkonservierungsmittel
US9932620B2 (en) 2012-05-21 2018-04-03 Celsis Ltd. Methods, devices, and systems of detecting microorganisms
JP6153461B2 (ja) * 2013-12-17 2017-06-28 株式会社ヤクルト本社 微生物の回収方法
USD809475S1 (en) * 2016-04-01 2018-02-06 Bose Corporation Set of headphones
GB201617713D0 (en) 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
CN109580928A (zh) * 2017-09-28 2019-04-05 中国人民解放军军事医学科学院放射与辐射医学研究所 原料奶三种快速前处理方法
WO2020160257A1 (en) * 2019-01-31 2020-08-06 Transformative Technologies A stall side method for the detection of bacteria in dairy cattle
CN110437983A (zh) * 2019-07-09 2019-11-12 南京格致医学检验有限公司 一种基于尿液沉淀煮沸的dna提取装置及方法

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Also Published As

Publication number Publication date
JP2867181B2 (ja) 1999-03-08
EP0542790A4 (es) 1995-02-08
US5700645A (en) 1997-12-23
US5587286A (en) 1996-12-24
WO1992000317A1 (en) 1992-01-09
EP0542790B1 (en) 1997-12-10
ATE161015T1 (de) 1997-12-15
JPH06500462A (ja) 1994-01-20
EP0542790A1 (en) 1993-05-26
DE69128425D1 (de) 1998-01-22
DE69128425T2 (de) 1998-04-09
AU8296691A (en) 1992-01-23
JPH04228097A (ja) 1992-08-18

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