ES2100051T3 - Sintesis de bloques dimeros y su uso en el ensamblaje de oligonucleotidos. - Google Patents
Sintesis de bloques dimeros y su uso en el ensamblaje de oligonucleotidos.Info
- Publication number
- ES2100051T3 ES2100051T3 ES94906568T ES94906568T ES2100051T3 ES 2100051 T3 ES2100051 T3 ES 2100051T3 ES 94906568 T ES94906568 T ES 94906568T ES 94906568 T ES94906568 T ES 94906568T ES 2100051 T3 ES2100051 T3 ES 2100051T3
- Authority
- ES
- Spain
- Prior art keywords
- dimer blocks
- oligonucleotides
- synthesis
- assembly
- blocks
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000539 dimer Substances 0.000 title abstract 4
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 2
- 230000015572 biosynthetic process Effects 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Saccharide Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
EN LA INVENCION SE PRESENTAN METODOS PARA LA SINTESIS DE BLOQUES DE DIMEROS (DERIVADOS DE DINUCLEOSIDOS QUE TIENE LOS ENLACES DE INTERNUCLEOSIDOS MODIFICADOS) UTILIZANDO TECNICAS QUIMICAS SIMPLIFICADAS, NUEVOS BLOQUES DE DIMEROS Y METODO PARA LA UTILIZACION DE BLOQUES DE DIMEROS PARA LA UNION DE OLIGONUCLEOTIDOS QUE TIENEN ENLACES DE INTERNUCLEOTIDOS MODIFICADOS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US282393A | 1993-01-08 | 1993-01-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2100051T3 true ES2100051T3 (es) | 1997-06-01 |
Family
ID=21702683
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES94906568T Expired - Lifetime ES2100051T3 (es) | 1993-01-08 | 1994-01-07 | Sintesis de bloques dimeros y su uso en el ensamblaje de oligonucleotidos. |
Country Status (13)
Country | Link |
---|---|
EP (1) | EP0678096B1 (es) |
JP (1) | JPH08507752A (es) |
KR (1) | KR960700262A (es) |
CN (1) | CN1122137A (es) |
AT (1) | ATE150464T1 (es) |
CA (1) | CA2153505A1 (es) |
DE (1) | DE69402177T2 (es) |
DK (1) | DK0678096T3 (es) |
ES (1) | ES2100051T3 (es) |
FI (1) | FI953363A0 (es) |
GR (1) | GR3023123T3 (es) |
NZ (1) | NZ261480A (es) |
WO (1) | WO1994015946A1 (es) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6087491A (en) * | 1993-01-08 | 2000-07-11 | Hybridon, Inc. | Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks |
AU5319396A (en) * | 1995-03-23 | 1996-10-08 | Hybridon, Inc. | Thiono triester modified antisense oligodeoxynucleotide phosphorothioates |
US5856464A (en) * | 1995-06-07 | 1999-01-05 | Lajolla Pharmaceutical Company | Selective capping solution phase oligonucleotide synthesis |
US6172217B1 (en) * | 1996-12-27 | 2001-01-09 | Isis Pharmaceuticals Inc. | Method of synthesizing phosphorothioate oligonucleotides |
US5760209A (en) * | 1997-03-03 | 1998-06-02 | Isis Pharmaceuticals, Inc. | Protecting group for synthesizing oligonucleotide analogs |
US5902881A (en) * | 1997-03-03 | 1999-05-11 | Isis Pharmaceuticals, Inc. | Reagent useful for synthesizing sulfurized oligonucleotide analogs |
GB9717158D0 (en) * | 1997-08-13 | 1997-10-22 | King S College London | Solution synthesis of oligonucleotides and their phosphorothioate analogues |
US6380378B1 (en) | 1998-12-24 | 2002-04-30 | Toagosei Company, Ltd. | Nucleotide compound, nucleotide block oligonucleotide, and method for producing them |
US6207819B1 (en) * | 1999-02-12 | 2001-03-27 | Isis Pharmaceuticals, Inc. | Compounds, processes and intermediates for synthesis of mixed backbone oligomeric compounds |
JP2003506566A (ja) | 1999-06-25 | 2003-02-18 | アカデミス ジーケンホイス ベイ デ ユニフェルジテイト ファン アムステルダム | ラジカルをウロカニン酸、誘導体、及びアナログで除去する方法 |
CN102344477B (zh) * | 2010-07-27 | 2015-04-08 | 苏州瑞博生物技术有限公司 | 一种核苷酸和/或寡核苷酸及其制备方法 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4469863A (en) * | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
DE3332068A1 (de) * | 1983-09-06 | 1985-03-21 | Hoechst Ag, 6230 Frankfurt | Verfahren zur herstellung von nukleosidalkyl-, aralkyl- und arylphosphoniten und -phosphonaten |
US4959463A (en) * | 1985-10-15 | 1990-09-25 | Genentech, Inc. | Intermediates |
FR2596761B1 (fr) * | 1986-04-08 | 1988-05-20 | Commissariat Energie Atomique | Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides |
US5194428A (en) * | 1986-05-23 | 1993-03-16 | Worcester Foundation For Experimental Biology | Inhibition of influenza virus replication by oligonucleotide phosphorothioates |
US5449769A (en) * | 1989-03-06 | 1995-09-12 | Gen-Probe Incorporated | Method and reagent for sulfurization of organophosphorous compounds |
US5003097A (en) * | 1989-10-02 | 1991-03-26 | The United States Of America As Represented By The Department Of Health And Human Services | Method for the sulfurization of phosphorous groups in compounds |
DK0942000T3 (da) * | 1989-10-24 | 2004-11-01 | Isis Pharmaceuticals Inc | 2'-modificerede oligonukleotider |
WO1992004358A1 (en) * | 1990-09-04 | 1992-03-19 | Gen-Probe Incorporated | Method and reagent for sulfurization of organophosphorous compounds |
WO1992020967A1 (en) * | 1991-05-10 | 1992-11-26 | Scholer Incineration Co Pty Ltd | Incinerator |
FI115214B (fi) * | 1992-01-22 | 2005-03-31 | Hoechst Ag | Menetelmä oligonukleotidianalogien valmistamiseksi ja niiden käyttö |
RU95104940A (ru) * | 1992-07-27 | 1997-01-10 | Хайбрайдон | Способ введения в олигонуклеотид алкилфосфонотиоатной или арилфосфонотиоатной межнуклеотидной связи, способ получения олигонуклеотида, олигонуклеотиды, способ ингибирования генной экспрессии, способ лечения |
-
1994
- 1994-01-07 WO PCT/US1994/000296 patent/WO1994015946A1/en active IP Right Grant
- 1994-01-07 EP EP94906568A patent/EP0678096B1/en not_active Expired - Lifetime
- 1994-01-07 CN CN94191181A patent/CN1122137A/zh active Pending
- 1994-01-07 DE DE69402177T patent/DE69402177T2/de not_active Expired - Fee Related
- 1994-01-07 AT AT94906568T patent/ATE150464T1/de not_active IP Right Cessation
- 1994-01-07 NZ NZ261480A patent/NZ261480A/en unknown
- 1994-01-07 DK DK94906568.4T patent/DK0678096T3/da active
- 1994-01-07 CA CA002153505A patent/CA2153505A1/en not_active Abandoned
- 1994-01-07 KR KR1019950702853A patent/KR960700262A/ko not_active Application Discontinuation
- 1994-01-07 ES ES94906568T patent/ES2100051T3/es not_active Expired - Lifetime
- 1994-01-07 JP JP6516255A patent/JPH08507752A/ja active Pending
-
1995
- 1995-07-07 FI FI953363A patent/FI953363A0/fi not_active Application Discontinuation
-
1997
- 1997-04-14 GR GR970400784T patent/GR3023123T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
CN1122137A (zh) | 1996-05-08 |
DK0678096T3 (da) | 1997-08-18 |
ATE150464T1 (de) | 1997-04-15 |
NZ261480A (en) | 1997-05-26 |
DE69402177D1 (de) | 1997-04-24 |
GR3023123T3 (en) | 1997-07-30 |
AU673051B2 (en) | 1996-10-24 |
AU6024394A (en) | 1994-08-15 |
CA2153505A1 (en) | 1994-07-21 |
EP0678096A1 (en) | 1995-10-25 |
FI953363A (fi) | 1995-07-07 |
DE69402177T2 (de) | 1997-06-26 |
EP0678096B1 (en) | 1997-03-19 |
JPH08507752A (ja) | 1996-08-20 |
WO1994015946A1 (en) | 1994-07-21 |
FI953363A0 (fi) | 1995-07-07 |
KR960700262A (ko) | 1996-01-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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