WO1992004358A1

WO1992004358A1 - Method and reagent for sulfurization of organophosphorous compounds

Info

Publication number: WO1992004358A1
Application number: PCT/US1990/004994
Authority: WO
Inventors: Ram S. Bhatt
Original assignee: Gen-Probe Incorporated
Priority date: 1990-09-04
Filing date: 1990-09-04
Publication date: 1992-03-19
Also published as: AU6523990A; AU642673B2

Abstract

A composition suitable for sulfurizing an organophosphite. The reagent comprises a solution of sulfur and a tertiary amine, in a suitable solvent such as carbon disulfide. The tertiary amine is preferably an optionally substituted trialkyl amine, for example triethylamine or diisopropylamine. A method is also provided in which phosphites or phosphonous esters are sulfurized to their corresponding phosphorothioates or phosphonothioates, respectively, using compositions of the present invention. This method has particular application to produce internucleotide phosphorothioate or phosphonothioate bonds in a nucleotide multimer.

Description

DESCRIPTION

METHOD AND REAGENT FOR SULFURIZATION OF ORGANOPHOSPHOROUS COMPOUNDS

Field of the Invention

This invention relates to the sulfurization of phosphites and phosphonous esters. In another aspect it relates to phosphorothioate analogs of nucleic acids. In another aspect it relates to methods for synthesizing such analogs. In a further aspect it relates to reagents useful in the sulfurization of phosphites and phosphonous esters, for example, the synthesis of phosphorothioate analogs of nucleic acids.

gacKqround of the Invention

Nucleic Acids occur in nature as chains of either ribonucleotides or deoxyribonucleotides, the individual nucleotides being linked to each other by phosphodiester bonds between the ribose and deoxyribose sugars which form, respectively, the backbones of ribo- nucleic acids (RNA) or deoxyribonucleic acids (DNA) . Apart from their role in naturally occurring phenomena, DNA and RNA, particularly DNA and RNA oligonucleotides, are expected to play an increasingly important role in medical diagnostic and therapeutic applications. For example, oligonucleotides have been shown to be useful in a variety of "probe" assays for viral and bacterial diseases and for the detection of genetic abnormalities. In these assays, the "probe" is typically an oligonucleo- tide selected to be complementary to a RNA or DNA sequence which is unique to the organism or genetic defect to be detected (Matthews et al., Anal. Bioche .. Vol. 169, (1988)) . It has also been observed that oligonucleotides which are complementary to messenger RNA (antisense oligonucleotides) can be introduced to a cell and arrest the translation of the mRNA. This arrest is believed to result from the hybridization of the antisense oligonu- cleotide to the mRNA. See, for example, Stephenson, et al., Proc. Natl. Acad. Sci.. USA, 75, 285 (1978) and Za ecnick, et al., Proc. Natl. Acad. Sci.. USA, 75, 280 (1978) . The ability of antisense oligonucleotides to inhibit or prevent mRNA translation suggests their ap¬ plication in antiviral therapy. A virus infecting a cell reproduces its genetic information by using the biological machinery of the infected cell. Transcription and trans- lation of that information by the cellular ribosomes are essential to viral reproduction. Thus, if expression of the viral gene can be interrupted, the virus cannot replicate or may replicate at such a slow rate as to give the host's immune system a better opportunity to combat the infection.

It has been proposed to use oligonucleotides in viral therapy by designing an oligonucleotide with a nucleotide sequence complementary to a sequence of virally expressed mRNA which must be translated if viral replica- tion is to be successful. Introduction of the antisense oligonucleotide to the cell permits it to hybridize with and prevent, or at least inhibit, this essential transla¬ tion.

Conventional phosphodiester antisense oligonu- cleotides have been reported to exhibit significant shortcomings as antisense oligonucleotides. One limita¬ tion is that they are highly subject to degradation by nucleases, enzymes which breakdown nucleic acids to permit recycling of the nucleotides. In addition, most cells are negatively charged. As a result, a phosphodiester oligo¬ nucleotide does not readily penetrate the cell membrane because of the density of negative charge in the backbone of the oligonucleotide. It has been proposed to modify oligonucleotides to overcome these shortcomings. One such proposal has been to use non-polar analogs of conventional phospho¬ diester oligonucleotides. Such analogs retain the ability to hybridize with a complementary sequence and would be expected to enter the cell more readily and be less resistive to nuclease degradation. Promising results have been obtained with methyl phosphonate analogs. See Agris et al., Biochemistry 25. 1228 (1986). More recently phosphorothioate analogs, i.e., nucleic acids in which one of the non-bridging oxygen atoms in each inter-nucleotide linkage has been replaced by a sulfur atom, have also been shown to possess the ability to block mRNA translation. In at least one case, inhibition of expression of the chlora phenicol acetyltransferase gene, a phosphorothioate analog has been shown to be superior to the methyl phos¬ phonate analog which in turn was shown to be more effec¬ tive than the unmodified phosphodiester oligonucleotide. Inhibition of HIV virus replication by a thiophosphorate analog has also been demonstrated. See Matsukara et al, Proc. Natl. Acad. Sci.. USA, 84, 1 (1987).

Phosphorothioate analogs of oligonucleotide probes are also useful as replacements for conventional probes for diagnostic and therapeutic applications. However, only a few techniques have been reported for the synthesis of phosphorothioate analogs of nucleic acids, all of them cumbersome and not well adapted for use with currently available automated nucleic acid synthesizers. One reported synthetic technique, for example, uses presynthesized nucleotide di ers. The synthesis of the full array of sixteen dimers necessary for the proce¬ dure is both laborious and expensive. (Connoly et al., Biocheπt.. 23, 3443 (1984).

A more preferred procedure would permit use of the highly reactive, commercially available nucleoside- phosphoramidite monomers currently employed with nucleic acid synthesizers. Such monomers are actually used in processes for preparing phosphorothioate analogs. How¬ ever, the sulfurization of phosphorous in the phosphite intermediate has been very troublesome. For example, elemental sulfur in pyridine at room temperature requires up to 16 hours to produce the internucleotide phosphorothioate triester 12 as shown in Fig. I. (P.S. Nelson, et al., J. Orσ. Chem.. 49, 2316 (1984); P.M.S. Burgers, et al., Tet. Lett.. 40, 3835 (1978)). A similar procedure using elemental sulfur, pyridine and carbon disulfide permitted sulfurization to be done at room temperature within 2 hours. B.A. Connolly, et al., Biochem.. 23, 3443 (1984). The triester is convertible to the phosphorothioate . by base catalyzed removal of substituent "R."

Carrying out the sulfurization at 60*C in 2,6- lutidine requires 15 minutes during automated, solid phase synthesis of phosphothioates from Compound 11. .J. Stec et al., J. Am. Chem. Soc.. 106, 6077 (1984). However, most automated synthesizers do not have provisions for heating the column required for performing sulfurization at elevated temperature and vaporization of the solvent at 60*C would be expected to form bubbles in delivery lines which would reduce flow rates and even cause synthesis failures. Furthermore, even a fifteen minute reaction time for sulfurization after the addition of each nucleo- tide makes the procedure far from optimal. Accordingly, there has gone unmet a need for a process for the preparation of phosphothioate oligonucleo¬ tide analogs that is rapid and that lends itself to use on conventional DNA synthesizers.

Terminology

The following terms are used in this disclosure and claims:

Nucleotide: A subunit of a nucleotide acid consisting of a phosphate group, a 5 carbon sugar ring and nitrogen containing purine or pyrimidine ring. In RNA the

5 carbon sugar is ribose. In DNA, it is 2-deoxyribose.

The term also includes analogs of such subunits.

Nucleotide multimer; A chain of two or more nucleotides linked by phosphorodiester or phosphonodiester bonds, or analogs thereof.

Oligonucleotide; A nucleotide multimer generally about 10 to 125 nucleotides in length, but which may be greater than 125 nucleotides in length. They are usually obtained by synthesis from nucleotide monomers, but may also be obtained by enzymatic means.

Nucleotide multimer probe: A nucleotide multimer having a nucleotide sequence complementary with a target nucleotide sequence contained within a second nucleotide multimer, usually a polynucleotide. Usually the probe is selected to be perfectly complementary to the corresponding base in the target sequence. However, in some cases it may be adequate or even desirable that one or more nucleotides in the probe not be complementary to the corresponding base in the target sequence, or that various moieties of synthetic origin either replace a nucleotide within the probe or be inserted between bases of the probe. Typically, the probe is labeled when used for diagnostic purposes. Oligonucleotide probe: A probe of synthetic or enzymatic origin usually having less than about 125 nucleotides, but which may contain in excess of 200 nucleotides. Hybridization: The formation of a "hybrid", which is the complex formed between two nucleotide multi- mers by Watson-Crick base pairings between the complemen¬ tary bases.

Summary of the Invention The present invention then provides a reagent which is suitable for the convenient and efficient sul¬ furization of organic phosphites (i.e. trivalent phosphor¬ us bonded to three oxy groups with at least one thereof being an organic-oxy group) and organic phosphonous esters (i.e. trivalent phosphorous bonded to only two oxy groups) , to form the corresponding thiophosphorus acid derivatives (specifically, phosphorothioates or phosphononothioates, respectively) . The invention is particularly suited to sulfurizing a suitably protected inter-nucleotide, either 3'-5' or 2'-5* phosphite or phosphonous linkages contained in oligonucleotides and ribonucleotide multimers in solution or on a solid support for the manual or automated synthesis of phosphothioate oligonucleotides. Accordingly, the present invention provides, in one aspect, a reagent and process using that reagent suitable for adding "sulfur" to a phosphite or phosphonous intermediate such as the kind H (see Fig. 1 in which some preferred substituents are listed) to produce a phosorothioate or phosphonothioate of the kind 12. (see Fig. 1) . It should be noted that for compounds H the inter-nucleotide phosphite or phosphonous bond (and the inter-nucleotide phosphorothioate or phosphonothioate bond of 12.) is shown between the 3 '-hydroxyl of one nucleoside and the 5'-hydroxyl group of another nucleoside. However, those bonds can be between any two hydroxyl groups of two nucleosides, for example, between the 2' and 5* hydroxyls, or between the hydroxyl group of a nucleoside and that of a non-nucleoside moiety or even between two non-nucleoside moieties. The reagent comprises a mixture of elemental sulfur, a solvent for sulfur, and a tertiary alkyl amine. The preferred reagent is: 0.2M elemental sulfur in a mixture comprising 50% carbon disulfide and 50% diisopropylethylamine. Other reagents of the present invention include:

(i) 0.2M elemental sulfur in a mixture comprising 50% carbon disulfide and 50% triethyla ine; (ii) 0.2M elemental sulfur in a mixture comprising carbon disulfide (25%) , pyridine (25%) and triethylamine (50%) . The above reagents can rapidly sulfurize the phosphite or phosphonous intermediates of the kind IS at room temperature in just about 45 seconds, eliminating the need to choose between extended reaction times required for the sulfurization step if it is to be done at room temperature, or the use of elevated temperatures to achieve more rapid sulfurization as required by prior art processes. Fig. 3 illustrates a sulfurization process according to the invention as embodied in the synthesis of phosphorothioate analogs of oligonucleotides.

BRIEF DESCRIPTION OF THE DRAWINGS

Fig. 1 shows the sulfurization of an oligonu¬ cleotide phosphite or phosphonous ester to form a phosphorothioate triester, or phosphonothioate diester, of a nucleic acid, some preferred substituents listed.

Fig. 2 illustrates the formation of an oligonu¬ cleotide phosphite triester or phosphonous diester useful in the invention, with some preferred substituents listed.

Fig. 3 illustrates assembly of an oligonucleo¬ tide phosphorothioate, involving sulfurization of oligonu¬ cleotide phosphites using a reagent of the present inven¬ tion and its conversion to the corresponding phosphorothi- oate.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

As described above, the reagent of the present invention comprises a mixture of elemental sulfur, an organic solvent for sulfur, and a non-aromatic tertiary amine. By a "non-aromatic" amine is meant an amine which is not a member of an aromatic ring. However this term does not exclude an aromatic group bonded to the nitrogen of an amine group. The solvent for sulfur can be selected from solvents such as carbon disulfide, toluene, benzene or ethyl alcohol. Carbon disulfide is preferred as it is a particularly effective solvent for sulfur. The mixture can optionally include other substances, for example aromatic amines, such as pyridine. The useful tertiary amines include, for example, triethylamine and diisopro- pylethylamine. Diisopropylethylamine is preferred.

The composition of the reagent can vary over a wide range and the particular formulation which yields the best results can depend upon the phosphite or phosphonous ester being sulfurized. However, the most effective formulation of the reagent for a specific reaction can be determined by varying the components of the mixture. In addition, particular formulations may be found practically difficult to work with. For example, it was found that in a formulation in which l,8-diazabicyclo[5,4,0]undec-7-ene was used as the tertiary amine, sulfur precipitated out from the carbon disulfide solution.

The reagents of the present invention, then, are useful for the sulfurization of organic phosphites or phosphonous esters to form the corresponding phosphorothioate. The starting organophosphorus compound, and its corresponding phosphorothioate produced by the method of the present invention, have the formulae:

phosphite or phosphorothioate triester or phosphonous phosphonothioate diester

In the above formulae, R_1f R₂, and R₃ may be the same or different and are preferrably selected from organic moieties such as optionally substituted alkyl, alkoxy, phenyl, phenoxy, and tertiary a ino, and analogues of the foregoing. In particular, R, and R_j may be ribonucleosides and deoxyribonucleosides.

The reagents of the present invention are particularly useful in the synthesis of phosphorothioate analogs of oligonucleotides from a phosphite or phospho¬ nous ester in which R₁ and R_? are nucleosides, particularly suitably protected nucleosides. In the case where it is desired to synthesize simply a phosphorothioate analog of a nucleotide multimer, then R₃ is a group which can be selectively removed (cleaved) following sulfurization. Examples of such groups include methoxy, and β-cyanoethoxy. However, if it is desired to produce a phosphonothioate analog of a nucleotide multimer (i.e., an analog in which a phosphonous linking group has an 0» replaced with S=) , then R₃ need not be a group which can be selectively removed following sulfurization. For example, R₃ could be methyl. Phosphites and phosphonous esters of the foregoing type are readily obtained using the processes of U.S. Patent No. 4.625,677 and U.S. Patent No. 4,458,066 (the disclosures of these patents, and all other references cited herein, being incorporated into this application by reference) .

Figure 2 illustrates the assembly of a dinucleo- side phosphite or phosphonous ester 15. from compounds of the type 13. (phosphoramidites or phosphona idites) and 14 (nucleotides) . However, it will be appreciated that higher oligomers can be obtained by sequential additions of phosphoramidites according to known procedures. Sulfurization is preferably carried out after the addition of each nucleoside although sulfurization may be deferred and carried out on the assembled oligomer. A preferred reagent for the sulfurization of phosphites or phosphonous esters, particularly the internucleotides obtained from the phosphoramidite process, is 0.2M elemental sulfur dissolved in an equal volume mixture of carbon disulfide and diisopropylethylamine.

Examples 1 and 2 below describe in detail the use of a sulfurizing reagent of the present invention for automated synthesis of phosphorothioates of a different nucleotide multimers. Examples 3 and 4 describe the use of a different sulfurizing reagent of the present invention for automated synthesis of nucleotide multimers. Examples 5 and 6 are similar to Examples 1 through 4 but illustrate the use of different reagents. Example 7 describes the use of a further sulfurizing agent of the present invention for automated synthesis of various phosphorothioate nucleotide multimers, while Example 8 is similar to Example 7 but describes the use of different reagents. Examples 9 through 11 also describe the use of sulfurizing reagents of the present invention in the automated synthesis of various nucleotide multimers. Example 12 illustrates the Tm (melting temperature) of hybrids formed by the hybridization of phosphorothioate analogs with a complementary RNA sequence. Example 1

Automated Synthesis of Phosphorothioate Analog of TTTT Figure 3 illustrates the scheme for the syn¬ thesis of the phosphorothioate analog of TTTT

Materials: Gold label elemental sulfur, carbon disulfide and diisopropylethylamine ("DIEA") were purchased from Aldrich Fine Chemicals, Milwaukee, Wiscon- sin. Nucleoside phosphoramidites, 15-umol T-column, Tetrazole and Cap B (10% dimethylamino pyridine (DMAP) in tetrahydrofuran (THF) were purchased from Biosearch, Inc., San Rafael, California. Cap A (10% acetic anhydride (AC₂0) in THF) was prepared from distilled acetic anhydride (Aldrich). 2.5% trichloroacetic acid in dichloromethane was prepared from gold label trichloroacetic acid (Aldrich) . Acetonitrile, dichloromethane and tetrahydro¬ furan, all containing less than 0.002% water were pur¬ chased from J.T. Baker. The scheme of Figure 3 outlines the steps involved in the automated synthesis of a nucleotide tetra er involving automated sulfurization of the phos¬ phite intermediate 2. Synthesis was performed on Bio¬ search 8750 DNA synthesizer using a modified version of the standard synthesis cycle. This modification makes use of an aqueous wash (10% water in 2% pyridine-THF) step prior to the oxidation step. This modification was included to minimize side reactions in the overall syn¬ thesis. Sulfurization was achieved by delivering a 0.2 molar solution of elemental sulfur in 50% carbon disulfide/ 50% diisopropylethylamine, (all percentages by volume unless otherwise indicated) for 15 seconds and letting it stand in the 15 μmol T-column for 30 seconds. After the sulfurization, the column and the delivery lines were washed with acetonitrile before beginning the next cycle of addition of the next base of the sequence.

As shown in Figure 3, the synthetic procedure is initiated using 5'-O-dimethoxytrityl-thymidine linked to controlled pore glass (CPG) , reagent 16, as described, for example, in U.S. Patent No. 4,458,066. The 5'-dimethoxy- trityl (DMT) protecting group is removed using acid catalysis to form the deprotected intermediate 12 which is coupled to the thymidine phosphoramidite 18 to form dimer 1£. Any unreacted 12 is capped by treatment with acetic anhydride and dimethylaminopyridine. The dimer 12 is sulfurized with the same sulfurization reagent consisting of 0.2M sulfur in 50% CS₂/50% DIEA to form the phosphorothioate triester 22._' The 5'-protecting group is then removed as before to form dimer 21 and the trimer (not shown) assembled using the thymidine phosphoramidite 18 which is then sulfurized and deprotected, followed by assembly of a tetramer using thymidine phosphoramidite 18. Sulfurization of the tetramer and removal of the 5'- protecting group in the same manner as described, provides tetramer 22.

After tetramer 22. bad been assembled, the resin from the column was transferred into a screw-cap tube and heated with concentrated ammonium hydroxide at 55" for 8- 10 hours to form 22._' Ammonium hydroxide was then evapor¬ ated off to give 300 OD units of the product. An analyti¬ cal examination of the purity was performed by gel elec- trophoresis of 2 OD units of the crude product on a 10% acrylamide gel containing 7M urea. The product was largely one band (ca, 95%) as shown by UV shadowing.

The extent to which the "sulfur" had been added to the "phosphorus" of the phosphite intermediate was determined by ³¹P nuclear magnetic resonance (NMR) in D₂0. Absence of ³¹P resonance at -2.688 PPM in the NMR spectrum of the phosphorothioate analog of T-T-T-T (³¹P, 53.866 PPM) indicates that the extent of sulfurization is almost quantitative. Example 2

The use of the sulfurization reagent was then extended to the synthesis of a phosphorothioate analog of a longer nucleotide multimer. This longer multimer was synthesized and deblocked the same way as described in Example 1 but purified by gel electrophoresis (20% acryla¬ mide - 7M urea) . The product was visualized though UV shadowing, the slow moving band was sliced off and ex¬ tracted with 0.1M ammonium acetate (pH 7.2) and desalted on sep-pak followed by a second desalting on Pharmacia's Nap-25 column. Such a desalted multimer was labeled with δ-ATP³² and gel electrophoresed to check the analytical purity. The following phosphorothioate multimer was synthesized (the designation "PS" before the 5' end of the multimer sequence indicating the phophorous of all nucleoside linking groups has been sulfurized) :

A PS-GCTCGTTGCGGGACTTAACCCAACAT ("26-mer") The phosphorothioate analog of the multimer moved very closely in gel electrophoresis to the corresponding normal multimer. Example 3

The procedure of Example 1 was repeated except using as a sulfurization reagent, 0.2M sulfur in 50% CS₂/50% triethylamine ("TEA"), to successfully produce PS-TTTT. Exa ple 4

The procedure of Example 2 was repeated except using as a sulfurization reagent 0.2M sulfur in 50% CS₂/50% TEA. In both this Example and in Example 3, the respective sulfurized nucleotide multimers were obtained. However, the sulfurization reagent of Examples 1 and 2 is preferred over that of this Example and Example 3, since it was found that when TEA was used as the tertiary amine rather than DIEA, a brown glue like substance started appearing in the flask after about 6 hours, which was not the case when DIEA was used (the S/CS₂/DIEA showing no signs of instability even after 3 days) . Example 5

The procedure of Examples 1 and 2 were repeated to again successfully produce PS-TTTT, and a. (the 26-mer) , but using the methoxy, rather than the β-cyanoethoxy, phosphoramidites of the nucleosides of the formula:

isopropyl

imethoxytrityl (4,4'- dimethoxytriphenyl- ethyl)

Example 6

The procedures of Examples 3 and 4 were repeated to again successfully produce the PS-TTTT and a. (the 26 er) , but using the methoxy phosphoramidites of Example 5. Example 7

A sulfurization reagent was prepared consisting of 0.2M S in 25% CS₂/25% pyridine/50% TEA. The procedure of Example 1 was then repeated except using the foregoing sulfurization reagent. The procedure of Example 2 was also repeated, to prepare a (the 26-mer) , using the foregoing sulfurization reagent. In addition, using the foregoing sulfurization reagent and the procedure of

Example 2, the following nucleotide multimers were also successfully prepared.

b PS-TTAACCCAACAT (12-mer) c PS-GACTTAACCCAACTAT (15-mer) d PS-GCTCGTTGCGGGACTTAACCC (21-mer) Example 8

The procedure of Example 7 was repeated, but using the methoxy phosphoramidites of the nucleotides, to successfully prepare the sulfurized oligomers a., _, c., and d. Example 9

The procedure of Example 1 was again essentially repeated except using the methyl, rather than the β- cyanoethoxy, phosphonamidite of the T-nucleoside having the formula:

isopropyl

In addition, the sulfurization reagent used was 0.2M sulfur in 25% CS₂/25% pyridine/50% TEA. Further, in order to cleave the PS-nucleotide from the resin, the resin was treated at room temperature with 1 ml of a 1:1 (by volume) mixture of ethylenediamine/EtOH for 6 hours.

The resulting product was PS-TTTT in which the nucleosides are linked by methyl phosphonodiester groups. Example 10 The procedure of Example 2 was essentially repeated to prepare the sulfurized 15-mer of c, except using the methyl phosphonamidite and the cleavage procedure Example 9. The resulting oligomer has the sequence c in which the nucleosides are linked by methyl phosphonothioate diester groups. Example 11

Example 9 was repeated except the sulfurizing reagent used as 0.2M sulfur in 50% CS₂/50% DIEA (which as previously mentioned is preferred over that using TEA, or that in which pyridine is additionally present) . The resulting product was again the PS-TTTT oligomer in which the nucleotides are linked by methyl phosphonothioate diester groups of the formula: I

CH₃-P«S

Example 12

The table below compares the Tm of two phosphor¬ othioate analogs with normal nucleotide multimers. The P³² labeled nucleotide multimer was hybridized with E. coli RNA in 0.48 H phosphate buffer, the hybrid bound to hydroxyapatite resin and the thermal stability measured in 0.1J4 phosphate buffer.

Tm ^βc Normal Phosphoro ¬ thioate Multimer Mu timer

15-mer 41.8 40.3

26-mer 66. 1 50.9 The foregoing Examples illustrate specific applications of the invention. Other useful applications of the invention which may be a departure from the specific Examples will be apparent to those skilled in the art. Accordingly, the present invention is not limited to those examples described above.

Claims

1. A composition suitable for sulfurization of an organophosphite, comprising a solution of sulfur and a non-aromatic tertiary amine, in a solvent.

2. A composition as defined in claim 1 wherein the tertiary amine is a trialkyl amine.

3. A composition as defined in claim 2 wherein the solvent is an organic solvent.

4. A composition as defined in claim 1 or 2 wherein the solvent is carbon disulfide.

5. A composition as defined in claim 1 wherein the solvent is an organic solvent and the tertiary amine is selected from triethylamine and diisopropylethylamine.

6. A composition as defined in claim 1, 2 or 5 wherein the sulfur concentration is at least about 0.2 M.

7. A composition suitable for sulfurization of an organo-phosphite, comprising a solution of sulfur and a trialkyl amine in an organic solvent with substantially equal parts by weight of the trialkyl amine and the organic solvent.

8. A composition as defined in claim 7 wherein the organic solvent is carbon disulfide and the trialkyl amine is selected from triethylamine and diisopropylethylamine.

9. A method of sulfurizing an organic phospohorus compound selected from an organic phosphite and an organic phosphonous ester to obtain the corresponding organic thiophosphorus acid derivative, comprising contacting the organic phosphorus compound with a solution of sulfur and a non-aromatic tertiary amine, in a solvent.

10. A method as defined in claim 9 wherein the tertiary amine is a trialkyl amine.

11. A method as defined in claim 9 or 10 wherein the solvent is an organic solvent.

12. A method as defined in claim 9 or 10 wherein the solvent is carbon disulfide.

13. A method of sulfurizing an organic phospohorus compound selected from an organic phosphite triester and an organic organophosphonous diester to obtain the cor¬ responding organic thiophosphorus acid derivative, com¬ prising contacting the organic phosphorus compound with a solution of sulfur and a non-aromatic tertiary amine, in a solvent.

14. A method as defined in claim 13 wherein the organic phosphorous compound is selected from a dinucleo- side phosphite triester and a dinucleoside organophospho- nous diester.

15. A method as defined in claim 13 or 14 wherein the tertiary amine is a trialkyl amine.

16. A method as defined in claim 13 or 14 wherein the solvent is an organic solvent.

17. A method as defined in claim 13 or 14 wherein the solvent comprises carbon disulfide.

18. A method as defined in claim 13 or 14 wherein the solvent is an organic solvent and the tertiary amine is a trialkyl amine.

19. A method as defined in claim 14 wherein the solvent comprises carbon disulfide, the tertiary amine is a trialkyl amine.

20. A method as defined in claim 14 wherein the solution of sulfur and a trialkyl amine in the organic solvent has substantially equal parts by weight of the trialkyl amine and the organic solvent, and has a sulfur concentration of at least about 0.2 M.

21. A method as defined in claim 20 wherein the organic solvent is carbon disulfide and the trialkyl amine is selected from triethylamine and diisopropylethylamine.

22. A method of sulfurizing an organic phosphorus compound selected from compounds of the formula:

0R₁

R₃-P

0R₂ wherein R₁ and R_j are nucleosides and R₃ is optionally a group which can be selectively cleaved following sulfurization, comprising contacting the organic phosphorus compound with a composition comprising a solution of sulfur and a non-aromatic tertiary amine, in an organic solvent, to form a corresponding organic phosphorus thioester of the formula: OR,

R₃-P=S 0R₂

23. A method as defined in claim 22 wherein the tertiary amine is a trialkyl amine.

24. A method as defined in claim 23 wherein the solvent comprises carbon disulfide.

25. A method as defined in claim 24 wherein the tertiary amine is selected from triethylamine and diiso¬ propylethylamine.

26. A method as defined in claim 25 wherein the sulfur concentration is at least about 0.2 M.

27. A method as defined in claim 26 wherein the solution of sulfur and the trialkyl amine comprises substantially equal parts by weight of the trialkyl amine and the organic solvent.

28. A method as defined in claim 22, 23, or 27, wherein R₃ is selected from an optionally substituted alkyl, alkoxy, phenoxy, and tertiary amino, or analogues thereof.

29. A method as defined in claim 22, 23, or 27 wherein R_j is selected from methyl, methoxy, and β- cyanoethoxy.

30. A method as defined in claim 22, 23, or 27 additionally comprising cleaving the leaving group follow¬ ing sulfurization.

31. A method of producing a nucleotide multimer with at least two nucleosides linked by a sulfurized phosphoro¬ thioate or phosphonothioate bond, comprising linking a nucleoside phosphite or nucleoside phosphonite with a second nucleotide then contacting the resulting linked nucleosides with a mixture as defined in claim 1, 2, or 3 so as to sulfurize the internucleotide phosphite or phosphonous group to produce the corresponding inter- nucleotide phosphorothioate or phosphonothioate group.