DE69402177T2 - Synthese von dimer-blöcken und ihre verwendung bei der zusammensetzung von oligonukleotiden - Google Patents
Synthese von dimer-blöcken und ihre verwendung bei der zusammensetzung von oligonukleotidenInfo
- Publication number
- DE69402177T2 DE69402177T2 DE69402177T DE69402177T DE69402177T2 DE 69402177 T2 DE69402177 T2 DE 69402177T2 DE 69402177 T DE69402177 T DE 69402177T DE 69402177 T DE69402177 T DE 69402177T DE 69402177 T2 DE69402177 T2 DE 69402177T2
- Authority
- DE
- Germany
- Prior art keywords
- dimer blocks
- oligonucleotides
- synthesis
- composition
- blocks
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000539 dimer Substances 0.000 title abstract 4
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 2
- 230000015572 biosynthetic process Effects 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US282393A | 1993-01-08 | 1993-01-08 | |
| PCT/US1994/000296 WO1994015946A1 (en) | 1993-01-08 | 1994-01-07 | Synthesis of dimmer blocks and their use in assembling oligonucleotides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69402177D1 DE69402177D1 (de) | 1997-04-24 |
| DE69402177T2 true DE69402177T2 (de) | 1997-06-26 |
Family
ID=21702683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69402177T Expired - Fee Related DE69402177T2 (de) | 1993-01-08 | 1994-01-07 | Synthese von dimer-blöcken und ihre verwendung bei der zusammensetzung von oligonukleotiden |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP0678096B1 (de) |
| JP (1) | JPH08507752A (de) |
| KR (1) | KR960700262A (de) |
| CN (1) | CN1122137A (de) |
| AT (1) | ATE150464T1 (de) |
| CA (1) | CA2153505A1 (de) |
| DE (1) | DE69402177T2 (de) |
| DK (1) | DK0678096T3 (de) |
| ES (1) | ES2100051T3 (de) |
| FI (1) | FI953363A (de) |
| GR (1) | GR3023123T3 (de) |
| NZ (1) | NZ261480A (de) |
| WO (1) | WO1994015946A1 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6087491A (en) * | 1993-01-08 | 2000-07-11 | Hybridon, Inc. | Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks |
| WO1996029337A1 (en) * | 1995-03-23 | 1996-09-26 | Hybridon, Inc. | Thiono triester modified antisense oligodeoxynucleotide phosphorothioates |
| US5856464A (en) * | 1995-06-07 | 1999-01-05 | Lajolla Pharmaceutical Company | Selective capping solution phase oligonucleotide synthesis |
| US6172217B1 (en) | 1996-12-27 | 2001-01-09 | Isis Pharmaceuticals Inc. | Method of synthesizing phosphorothioate oligonucleotides |
| US5760209A (en) * | 1997-03-03 | 1998-06-02 | Isis Pharmaceuticals, Inc. | Protecting group for synthesizing oligonucleotide analogs |
| US5902881A (en) * | 1997-03-03 | 1999-05-11 | Isis Pharmaceuticals, Inc. | Reagent useful for synthesizing sulfurized oligonucleotide analogs |
| GB9717158D0 (en) * | 1997-08-13 | 1997-10-22 | King S College London | Solution synthesis of oligonucleotides and their phosphorothioate analogues |
| US6380378B1 (en) | 1998-12-24 | 2002-04-30 | Toagosei Company, Ltd. | Nucleotide compound, nucleotide block oligonucleotide, and method for producing them |
| US6207819B1 (en) * | 1999-02-12 | 2001-03-27 | Isis Pharmaceuticals, Inc. | Compounds, processes and intermediates for synthesis of mixed backbone oligomeric compounds |
| SI1196129T1 (sl) | 1999-06-25 | 2006-04-30 | Az Univ Amsterdam | Farmacevtski in kozmeticni sestavki, ki obsegajo derivate urokaninske kisline kot odstranjevalce radikalov ali antioksidante |
| CN102344477B (zh) * | 2010-07-27 | 2015-04-08 | 苏州瑞博生物技术有限公司 | 一种核苷酸和/或寡核苷酸及其制备方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4469863A (en) * | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| DE3332068A1 (de) * | 1983-09-06 | 1985-03-21 | Hoechst Ag, 6230 Frankfurt | Verfahren zur herstellung von nukleosidalkyl-, aralkyl- und arylphosphoniten und -phosphonaten |
| US4959463A (en) * | 1985-10-15 | 1990-09-25 | Genentech, Inc. | Intermediates |
| FR2596761B1 (fr) * | 1986-04-08 | 1988-05-20 | Commissariat Energie Atomique | Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides |
| US5194428A (en) * | 1986-05-23 | 1993-03-16 | Worcester Foundation For Experimental Biology | Inhibition of influenza virus replication by oligonucleotide phosphorothioates |
| US5449769A (en) * | 1989-03-06 | 1995-09-12 | Gen-Probe Incorporated | Method and reagent for sulfurization of organophosphorous compounds |
| US5003097A (en) * | 1989-10-02 | 1991-03-26 | The United States Of America As Represented By The Department Of Health And Human Services | Method for the sulfurization of phosphorous groups in compounds |
| EP0942000B1 (de) * | 1989-10-24 | 2004-06-23 | Isis Pharmaceuticals, Inc. | 2'-Modifizierte Oligonukleotide |
| WO1992004358A1 (en) * | 1990-09-04 | 1992-03-19 | Gen-Probe Incorporated | Method and reagent for sulfurization of organophosphorous compounds |
| WO1992020967A1 (en) * | 1991-05-10 | 1992-11-26 | Scholer Incineration Co Pty Ltd | Incinerator |
| FI115214B (fi) * | 1992-01-22 | 2005-03-31 | Hoechst Ag | Menetelmä oligonukleotidianalogien valmistamiseksi ja niiden käyttö |
| CA2140542A1 (en) * | 1992-07-27 | 1994-02-03 | Abeysingle Padmapriya | Oligonucleotide alkylphosphonothioates |
-
1994
- 1994-01-07 DE DE69402177T patent/DE69402177T2/de not_active Expired - Fee Related
- 1994-01-07 KR KR1019950702853A patent/KR960700262A/ko not_active Application Discontinuation
- 1994-01-07 CA CA002153505A patent/CA2153505A1/en not_active Abandoned
- 1994-01-07 DK DK94906568.4T patent/DK0678096T3/da active
- 1994-01-07 EP EP94906568A patent/EP0678096B1/de not_active Expired - Lifetime
- 1994-01-07 WO PCT/US1994/000296 patent/WO1994015946A1/en active IP Right Grant
- 1994-01-07 CN CN94191181A patent/CN1122137A/zh active Pending
- 1994-01-07 JP JP6516255A patent/JPH08507752A/ja active Pending
- 1994-01-07 NZ NZ261480A patent/NZ261480A/en unknown
- 1994-01-07 AT AT94906568T patent/ATE150464T1/de not_active IP Right Cessation
- 1994-01-07 ES ES94906568T patent/ES2100051T3/es not_active Expired - Lifetime
-
1995
- 1995-07-07 FI FI953363A patent/FI953363A/fi not_active Application Discontinuation
-
1997
- 1997-04-14 GR GR970400784T patent/GR3023123T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| FI953363A0 (fi) | 1995-07-07 |
| AU6024394A (en) | 1994-08-15 |
| DE69402177D1 (de) | 1997-04-24 |
| ES2100051T3 (es) | 1997-06-01 |
| EP0678096B1 (de) | 1997-03-19 |
| EP0678096A1 (de) | 1995-10-25 |
| ATE150464T1 (de) | 1997-04-15 |
| CN1122137A (zh) | 1996-05-08 |
| GR3023123T3 (en) | 1997-07-30 |
| NZ261480A (en) | 1997-05-26 |
| JPH08507752A (ja) | 1996-08-20 |
| KR960700262A (ko) | 1996-01-19 |
| DK0678096T3 (da) | 1997-08-18 |
| CA2153505A1 (en) | 1994-07-21 |
| AU673051B2 (en) | 1996-10-24 |
| WO1994015946A1 (en) | 1994-07-21 |
| FI953363A (fi) | 1995-07-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8327 | Change in the person/name/address of the patent owner |
Owner name: HYBRIDON, INC., CAMBRIDGE, MASS., US |
|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |