ES2061990T3 - Indicadores fluorescentes de calcio intracelular. - Google Patents

Indicadores fluorescentes de calcio intracelular.

Info

Publication number
ES2061990T3
ES2061990T3 ES89304872T ES89304872T ES2061990T3 ES 2061990 T3 ES2061990 T3 ES 2061990T3 ES 89304872 T ES89304872 T ES 89304872T ES 89304872 T ES89304872 T ES 89304872T ES 2061990 T3 ES2061990 T3 ES 2061990T3
Authority
ES
Spain
Prior art keywords
intracellular calcium
fluorescent indicators
hydrogen
compounds
indicators
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89304872T
Other languages
English (en)
Inventor
Robert Michael Demarinis
Katharine Ann Muirhead
Haralambos Efst Katerinopoulos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp
Application granted granted Critical
Publication of ES2061990T3 publication Critical patent/ES2061990T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/06—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings
    • C07D409/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Electroluminescent Light Sources (AREA)
  • Luminescent Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

COMPUESTOS DE FORMULA(I), EN LA QUE R ES H O ACETOXIMETILO; R1 ES METILO O ETILO; Z ES O, S O NH; Y Y ES NH O S; O DONDE R ES H, UNA SAL DE LI O NA K THEREOF. ESTOS COMPUESTOS SON UTILES COMO INDICADORES OPTICOS EN UN METODO DE MEDICION DE CA INTRACELULAR. SE DESCRIBE ADEMAS EL PROCESO PARA SU PRODUCCION.
ES89304872T 1988-05-19 1989-05-15 Indicadores fluorescentes de calcio intracelular. Expired - Lifetime ES2061990T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/196,654 US4849362A (en) 1988-05-19 1988-05-19 Fluorescent intracellular calcium indicators

Publications (1)

Publication Number Publication Date
ES2061990T3 true ES2061990T3 (es) 1994-12-16

Family

ID=22726283

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89304872T Expired - Lifetime ES2061990T3 (es) 1988-05-19 1989-05-15 Indicadores fluorescentes de calcio intracelular.

Country Status (6)

Country Link
US (1) US4849362A (es)
EP (1) EP0342891B1 (es)
JP (2) JPH0813812B2 (es)
AT (1) ATE102619T1 (es)
DE (1) DE68913586T2 (es)
ES (1) ES2061990T3 (es)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200345A (en) * 1989-08-16 1993-04-06 New York University Methods and apparatus for quantifying tissue damage, determining tissue type, monitoring neural activity, and determining hematocrit
US5501980A (en) * 1994-05-20 1996-03-26 Molecular Probes, Inc. Benzazolylcoumarin-based ion indicators
US5648270A (en) * 1995-02-06 1997-07-15 Molecular Probes, Inc. Methods of sensing with fluorescent conjugates of metal-chelating nitrogen heterocycles
US5459276A (en) * 1994-05-20 1995-10-17 Molecular Probes, Inc. Benzazolylcoumarin-based ion indicators for heavy metals
US5453517A (en) * 1992-02-25 1995-09-26 Molecular Probes, Inc. Reactive derivatives of bapta used to make ion-selective chelators
JP2970967B2 (ja) * 1991-11-20 1999-11-02 浜松ホトニクス株式会社 蛍光性プローブ試薬を用いた細胞内イオン濃度測定法
US5262330A (en) * 1992-02-27 1993-11-16 Miles Inc. Colorimetric methods and reagents for the assay of calcium in a test sample
JPH07194393A (ja) * 1992-08-24 1995-08-01 Hamamatsu Photonics Kk 生細胞内イオン濃度の３波長測光法のための定数最適化方法
US5409835A (en) * 1992-12-30 1995-04-25 The University Of Maryland At Baltimore Long-wavelength fluorescent probe compounds for calcium ions and their use in ratiometrically measuring calcium ion concentrations
US5294799A (en) * 1993-02-01 1994-03-15 Aslund Nils R D Apparatus for quantitative imaging of multiple fluorophores
US5383023A (en) * 1993-03-01 1995-01-17 Walleczek; Jan Method and apparatus for performing dual-beam dual-wavelength fluorescence spectrophotometric evaluation of a biological specimen
US5773227A (en) * 1993-06-23 1998-06-30 Molecular Probes, Inc. Bifunctional chelating polysaccharides
AT401823B (de) * 1993-09-30 1996-12-27 Avl Verbrennungskraft Messtech Optischer indikator zur bestimmung der aktivität eines ions in einer probe
US5516911A (en) * 1993-12-30 1996-05-14 The United States Of America As Represented By The Department Of Health And Human Services Fluorescent intracellular calcium indicators
US5888829A (en) * 1994-11-08 1999-03-30 Molecular Probes, Inc. Photolabile caged ionophores and method of using in a membrane separation process
RU2152023C1 (ru) * 1995-01-30 2000-06-27 Цирельников Николай Иванович Устройство для прямого определения количественных параметров клеточных структур
US5872243A (en) * 1995-06-30 1999-02-16 Molecular Probes, Inc. Caged nucleotides
IL121844A0 (en) * 1997-09-28 1998-02-22 Dpharm Ltd Lipophilic diesters of chelating agents
US7169922B2 (en) * 2000-08-04 2007-01-30 Invitrogen Corporation Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
GB2368843A (en) * 2000-11-08 2002-05-15 Leuven K U Res & Dev Non-porphyrin multipurpose contrast agents
US7579463B2 (en) 2000-12-20 2009-08-25 Life Technologies Corporation Crown ether derivatives
US6962992B2 (en) 2000-12-20 2005-11-08 Molecullar Probes, Inc. Crown ether derivatives
US20050069962A1 (en) 2001-10-12 2005-03-31 Archer Robert M Antibody complexes and methods for immunolabeling
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
US7445894B2 (en) * 2002-05-03 2008-11-04 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
US20040171034A1 (en) * 2002-05-03 2004-09-02 Brian Agnew Compositions and methods for detection and isolation of phosphorylated molecules
CA2483868A1 (en) * 2002-05-03 2004-05-21 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
AU2003270619A1 (en) * 2002-09-12 2004-04-30 Molecular Probes, Inc. Site-specific labeling of affinity tags in fusion proteins
US8445702B2 (en) * 2003-05-05 2013-05-21 Life Technologies Corporation Zinc binding compounds and their method of use
US20050250214A1 (en) * 2004-05-05 2005-11-10 Gee Kyle R Zinc binding compounds and their method of use
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
CA2445420A1 (en) 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US7727752B2 (en) * 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US7271265B2 (en) * 2003-08-11 2007-09-18 Invitrogen Corporation Cyanine compounds and their application as quenching compounds
DE602004025592D1 (de) * 2003-09-12 2010-04-01 Life Technologies Corp Anwendungen des Resonanzenergietransfers zwischen Terbium und GFP
US7282339B2 (en) * 2003-09-17 2007-10-16 Invitrogen Corporation Competitive immunoassay
US20050214807A1 (en) * 2003-11-19 2005-09-29 Iain Johnson Environmental sensitive fluorogenic compounds and their application for singlet oxygen and protein detection
EP1720944B1 (en) * 2003-12-05 2013-07-17 Life Technologies Corporation Cyanine dye compounds
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
WO2005071096A2 (en) 2004-01-21 2005-08-04 Molecular Probes, Inc. Derivatives of cephalosporin and clavulanic acid for detecting beta-lacamase in a sample
US20060024833A1 (en) 2004-07-27 2006-02-02 Molecular Probes, Inc. Fluorescent metal ion indicators with large stokes shift
US7598390B2 (en) * 2005-05-11 2009-10-06 Life Technologies Corporation Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use
US20070134685A1 (en) * 2005-09-06 2007-06-14 Invitrogen Corporation Control of chemical modification
WO2007047450A1 (en) * 2005-10-13 2007-04-26 Aureon Laboratories, Inc. Multiplex in situ immunohistochemical analysis
US7521577B2 (en) * 2006-01-12 2009-04-21 Molecular Probes, Inc. Heavy metal binding compounds and their method of use
US20070196860A1 (en) * 2006-01-18 2007-08-23 Invitrogen Corporation Methods for Measuring Real Time Kinase Activity
JP2009524832A (ja) 2006-01-24 2009-07-02 ライフ テクノロジーズ コーポレーション 検体を定量するためのデバイスおよび方法
WO2007090126A2 (en) * 2006-01-30 2007-08-09 Invitrogen Corporation Compositions and methods for detecting and quantifying toxic substances in disease states
EP1987068B1 (en) 2006-02-10 2018-08-08 Life Technologies Corporation Oligosaccharide modification and labeling of proteins
US8114636B2 (en) 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
US8562802B1 (en) 2006-02-13 2013-10-22 Life Technologies Corporation Transilluminator base and scanner for imaging fluorescent gels, charging devices and portable electrophoresis systems
EP2362224A3 (en) 2006-03-23 2012-03-14 Absorber AB Blood group antigens of different types for diagnostic and therapeutic applications
EP2029799B1 (en) 2006-04-20 2012-08-22 Becton, Dickinson and Company Thermostable proteins and methods of making and using thereof
WO2008076524A2 (en) 2006-10-27 2008-06-26 Life Technologies Corporation Fluorogenic ph sensitive dyes and their method of use
US8586743B2 (en) 2007-01-30 2013-11-19 Life Technologies Corporation Labeling reagents and methods of their use
US20080254498A1 (en) * 2007-04-13 2008-10-16 Abd Bioquest, Inc. Fluorescent ion indicators and their applications
US9097730B2 (en) 2007-04-13 2015-08-04 Aat Bioquest, Inc. Fluorescein lactone ion indicators and their applications
US9279817B2 (en) 2007-04-13 2016-03-08 Aat Bioquest, Inc. Carbofluorescein lactone ion indicators and their applications
WO2008151089A2 (en) * 2007-05-30 2008-12-11 Invitrogen Corporation Fluorescent phospholipase a2 indicators
JP4923298B2 (ja) * 2007-09-07 2012-04-25 コリア ユニバーシティ インダストリアル アンド アカデミック コラボレイション ファウンデーション 細胞内カルシウムのリアルタイムモニタリング用二光子プローブ
CN104263816B (zh) * 2008-05-16 2018-10-19 生命技术公司 用于测量细胞增殖的双标记法
JP2012519170A (ja) 2009-02-26 2012-08-23 オーエスアイ・ファーマシューティカルズ，エルエルシー 生体内の腫瘍細胞のｅｍｔステータスをモニターするためのｉｎｓｉｔｕ法
JP5906190B2 (ja) 2009-10-17 2016-04-20 アサンテ リサーチ, エルエルシーAsante Research, Llc レシオメトリックであり、かつ赤色スペクトルで発光する蛍光カルシウム指示薬
US8974651B2 (en) 2010-04-17 2015-03-10 C.C. Imex Illuminator for visualization of fluorophores
WO2012022734A2 (en) 2010-08-16 2012-02-23 Medimmune Limited Anti-icam-1 antibodies and methods of use
CN104704359A (zh) 2012-10-25 2015-06-10 生命科技公司 糖蛋白酶介导位点特异性放射性标记的方法和组合物
WO2015050959A1 (en) 2013-10-01 2015-04-09 Yale University Anti-kit antibodies and methods of use thereof
JP6535675B2 (ja) 2013-10-02 2019-06-26 メディミューン，エルエルシー 抗ａ型インフルエンザ抗体の中和及びその使用
US10542918B2 (en) 2013-10-23 2020-01-28 Verily Life Sciences Llc Modulation of a response signal to distinguish between analyte and background signals
US9504405B2 (en) 2013-10-23 2016-11-29 Verily Life Sciences Llc Spatial modulation of magnetic particles in vasculature by external magnetic field
US9835587B2 (en) 2014-04-01 2017-12-05 C.C. Imex Electrophoresis running tank assembly
WO2016111915A1 (en) 2015-01-06 2016-07-14 De Haas Anthony H Near-infrared fluorescent surgical dye markers
US9861710B1 (en) 2015-01-16 2018-01-09 Verily Life Sciences Llc Composite particles, methods, and in vivo diagnostic system
US10028659B2 (en) 2015-03-26 2018-07-24 Verily Life Sciences Llc Aptamer-based sensors, implantable devices and detection system
WO2017181176A1 (en) 2016-04-15 2017-10-19 Lcahn School Of Medicine At Mount Sinai Tissue profiling using multiplexed immunohistochemical consecutive staining
US20210108253A1 (en) 2017-03-30 2021-04-15 Life Technologies Corporation Quantification of ngs dna by adapter sequence
US9810700B1 (en) 2017-05-31 2017-11-07 Aat Bioquest, Inc. Fluorogenic calcium ion indicators and methods of using the same
KR20230034333A (ko) 2020-07-02 2023-03-09 라이프 테크놀로지스 코포레이션 트리뉴클레오티드 캡 유사체, 제조 및 이의 용도
EP4373890A1 (en) 2021-07-21 2024-05-29 Life Technologies Corporation Dibenzoxanthene quenchers, uses, and methods of preparation
US12523648B1 (en) * 2025-02-13 2026-01-13 ION Biosciences Fluorescent indicators for calcium metal ion detection

Also Published As

Publication number Publication date
DE68913586D1 (de) 1994-04-14
JP2648293B2 (ja) 1997-08-27
US4849362A (en) 1989-07-18
ATE102619T1 (de) 1994-03-15
EP0342891A1 (en) 1989-11-23
JPH0813812B2 (ja) 1996-02-14
JPH08178850A (ja) 1996-07-12
EP0342891B1 (en) 1994-03-09
JPH0222275A (ja) 1990-01-25
DE68913586T2 (de) 1994-06-23

Similar Documents

Publication Publication Date Title
ES2061990T3 (es) Indicadores fluorescentes de calcio intracelular.
PT87552A (pt) Process for the preparation of novel tetrazole intermediates toantihypertensive compounds
EP0742209A3 (en) Process for the preparation of an optically active quinoline demevalonolacton and process for the preparation of a salt of an optically active quinoline demevalolon
DK633088A (da) Kollagenaseinhibitorer
ATE21105T1 (de) 7-oxo-4-thia-1-aza(3.2.0)heptan- und 7-oxo-4-thia-1-aza-(3.2.0)hept-2-en-derivate.
EP0031567A3 (en) Tetrapeptidesemicarbazide derivatives and their production and use
DE3882895D1 (de) Imid-derivate, ihre herstellung und verwendung.
ATE10838T1 (de) 4-substituierte 2-oxoazetidin-verbindungen, verfahren zu deren herstellung und ihre verwendung zur antibiotika-herstellung.
DE69416191D1 (de) Spiropyran-Derivate mit photochromischen Eigenschaften
DE3864707D1 (de) Benzoxazin-derivate und verfahren zu ihrer herstellung.
NO873564L (no) Nye tyroninderivater.
ES2053707T3 (es) Procedimiento para la preparacion de amidas de acidos 3,3-diaril-acrilicos.
GR1001302B (el) Μεθοδος παρασκευης υποκατεστημενων εθενιων.
IT1237529B (it) Composti spiro-indolino-ossazine con caratteristiche fotocromatiche e fotosensibilizzatrici e procedimento per la loro preparazione
DE3460467D1 (en) Hydroquinone derivatives and production thereof
ATE10370T1 (de) Benzothiazinderivate, verfahren zu ihrer herstellung und ihre verwendung.
DE3662048D1 (de) Novel thyronine derivatives
EP0309262A3 (en) Novel salicylates, their salts, pharmaceutical compositions containing them and process for preparing same
DK0745680T3 (da) Nye fungicide midler LL-15G256 gamma, delta, og epsilon produceret af LL-15G256 (Hypoxylon oceanicum)
DE69026098D1 (de) Organogermaniumderivate enthaltende Zubereitungen zur therapeutischen Verwendung
ES473453A1 (es) Un metodo para la preparacion de clorhidrato de cloruro de d-2-(p-hidroxifenil)glicilo dioxano hemisolvato
ES2164258T3 (es) Procedimiento de preparacion de derivados de carbamoilmetilurea.
BG50501A3 (bg) Метод за получаване на 1,5-бензтиазепинови производни
ES459649A1 (es) Un procedimiento para la produccion de un derivado de diaza-bicicloalcano.
IT7851816A0 (it) Benzossazolil-fenilstilbeni procedimento per la loro produzione nonche' loro applicazione come sbiancanti ottici

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 342891

Country of ref document: ES