ES2061619T3 - Metodo mejorado para la preparacion de la hormona de crecimiento humano natural en forma pura. - Google Patents

Metodo mejorado para la preparacion de la hormona de crecimiento humano natural en forma pura.

Info

Publication number
ES2061619T3
ES2061619T3 ES88121370T ES88121370T ES2061619T3 ES 2061619 T3 ES2061619 T3 ES 2061619T3 ES 88121370 T ES88121370 T ES 88121370T ES 88121370 T ES88121370 T ES 88121370T ES 2061619 T3 ES2061619 T3 ES 2061619T3
Authority
ES
Spain
Prior art keywords
preparation
improved method
pure form
growth hormone
human growth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88121370T
Other languages
English (en)
Inventor
Guido Grandi
Elisabetta Franchi
Federico Maisano
Testori Silvia Astrua
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eni Tecnologie SpA
Original Assignee
Eniricerche SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche SpA filed Critical Eniricerche SpA
Application granted granted Critical
Publication of ES2061619T3 publication Critical patent/ES2061619T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Child & Adolescent Psychology (AREA)
  • Dermatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE DESCRIBE UN METODO MEJORADO PARA LA PREPARACION DE HORMONA NATURAL HUMANA DE DESARROLLO (HGH) EN FORMA PURA, LA CUAL COMPRENDE LA PREPARACION DE UN PRECURSOR DE HGH POR MEDIO DEL CULTIVO DE CELULAS DE BACILOS DELICADOS TRANSFORMADOS POR UN PLASMA HIBRIDO, LA SEPARACION DEL PRECURSOR DE LAS PROTEINAS TOTALES, LA DIGESTION DEL PRECURSOR CON EL ENCIMA FACTOR XA Y FINALMENTE LA PURIFICACION DE LA HGH NATURAL ASI OBTENIDA DE LA HIDROLISIS DE LA MEZCLA ENCIMATICA. LA HORMONA DE DESARROLLO ASI PREPARADA TIENE UNA PUREZA DEL 100% O APROXIMADAMENTE 100% Y SE USA EN PARTICULAR PARA TRATAMIENTO HUMANO.
ES88121370T 1987-12-22 1988-12-21 Metodo mejorado para la preparacion de la hormona de crecimiento humano natural en forma pura. Expired - Lifetime ES2061619T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT23148/87A IT1223577B (it) 1987-12-22 1987-12-22 Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura

Publications (1)

Publication Number Publication Date
ES2061619T3 true ES2061619T3 (es) 1994-12-16

Family

ID=11204294

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88121370T Expired - Lifetime ES2061619T3 (es) 1987-12-22 1988-12-21 Metodo mejorado para la preparacion de la hormona de crecimiento humano natural en forma pura.

Country Status (7)

Country Link
US (1) US5047333A (es)
EP (1) EP0321940B1 (es)
JP (1) JPH022386A (es)
AT (1) ATE101649T1 (es)
DE (1) DE3887856T2 (es)
ES (1) ES2061619T3 (es)
IT (1) IT1223577B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ236819A (en) * 1990-02-03 1993-07-27 Max Planck Gesellschaft Enzymatic cleavage of fusion proteins; fusion proteins; recombinant dna and pharmaceutical compositions
EP0564531B1 (en) * 1990-12-03 1998-03-25 Genentech, Inc. Enrichment method for variant proteins with altered binding properties
US5780279A (en) * 1990-12-03 1998-07-14 Genentech, Inc. Method of selection of proteolytic cleavage sites by directed evolution and phagemid display
IT1244477B (it) * 1990-12-21 1994-07-15 Eniricerche Spa Ceppo asporigeno di bacillus subtilis e suo impiego come ospite per la preparazione di prodotti eterologhi
IT1245385B (it) * 1991-03-28 1994-09-20 Eniricerche Spa Procedimento migliorato per la preparazione di polipeptidi maturi
IT1251895B (it) * 1991-09-27 1995-05-26 Eniricerche Spa Mutanti dell'ormone della crescita umano e loro impiego
IT1270866B (it) * 1993-03-10 1997-05-13 Eniricerche Spa Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilis
WO1999045020A1 (en) * 1998-03-04 1999-09-10 Bio-Technology General Corp. Isolation of tissue specific peptide ligands and their use for targeting pharmaceuticals to organs
AU2004320986A1 (en) * 2004-06-23 2006-01-05 Usv Limited Chimeric human growth hormone derived from the placenta and pituitary isoform and processes for obtaining said chimera
CA2565209A1 (en) * 2004-11-09 2006-05-18 Usv Limited A novel process for purification of human growth harmone
KR101527528B1 (ko) * 2012-09-19 2015-06-19 한국표준과학연구원 가용성 재조합 단백질의 생산, 추출 및 정제 방법

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
DK55685A (da) * 1985-02-07 1986-08-08 Nordisk Gentofte Enzym eller enzymkompleks med proteolytisk aktivitet
GB8412517D0 (en) * 1984-05-16 1984-06-20 Nagai K Recombinant fusion proteins
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano

Also Published As

Publication number Publication date
ATE101649T1 (de) 1994-03-15
EP0321940A3 (en) 1990-05-23
DE3887856D1 (de) 1994-03-24
US5047333A (en) 1991-09-10
IT1223577B (it) 1990-09-19
IT8723148A0 (it) 1987-12-22
EP0321940B1 (en) 1994-02-16
JPH022386A (ja) 1990-01-08
EP0321940A2 (en) 1989-06-28
DE3887856T2 (de) 1994-05-19

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