ES2056082T3 - Nuevo activador de plasminogeno de tipo tisular. - Google Patents

Nuevo activador de plasminogeno de tipo tisular.

Info

Publication number
ES2056082T3
ES2056082T3 ES88112569T ES88112569T ES2056082T3 ES 2056082 T3 ES2056082 T3 ES 2056082T3 ES 88112569 T ES88112569 T ES 88112569T ES 88112569 T ES88112569 T ES 88112569T ES 2056082 T3 ES2056082 T3 ES 2056082T3
Authority
ES
Spain
Prior art keywords
plasminogen activator
new tissue
tissue type
type plasminogen
plasmin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88112569T
Other languages
English (en)
Inventor
Mineo Niwa
Yoshimasa Saito
Hitoshi Sasaki
Masako Hayashi
Jouji Notani
Masakazu Kobayashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujisawa Pharmaceutical Co Ltd
Original Assignee
Fujisawa Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878718298A external-priority patent/GB8718298D0/en
Priority claimed from GB878725052A external-priority patent/GB8725052D0/en
Priority claimed from GB878726683A external-priority patent/GB8726683D0/en
Application filed by Fujisawa Pharmaceutical Co Ltd filed Critical Fujisawa Pharmaceutical Co Ltd
Application granted granted Critical
Publication of ES2056082T3 publication Critical patent/ES2056082T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cardiology (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

ESTA INVENCION REVELA UN NUEVO TEJIDO ACTIVADOR DEL PLASMINOGENO QUE PRESENTA UNA ACTIVIDAD ELEVADA EN LA CONVERSION DEL PLASMINOGENOGENO EN PLASMINA, LA CUAL REALIZA LA DEGRADACION DE LAS REDES DE FIBRINA EN LOS COAGULOS SANGUINEOS PARA FORMAR PRODUCTOS SOLUBLES, Y ES POR LO TANTO MUY UTIL COMO AGENTE TRAMBOLITICO. TAMBIEN SE DESCRIBE UN FRAGMENTO DE DNA QUE CODIFICA SU SECUENCIA DE AMINOACIDOS, UN PROCESO PARA SU PRODUCCION Y UN PREPARADO FARMACEUTICO QUE LO INCLUYE COMO COMPONENTE.
ES88112569T 1987-08-03 1988-08-02 Nuevo activador de plasminogeno de tipo tisular. Expired - Lifetime ES2056082T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB878718298A GB8718298D0 (en) 1987-08-03 1987-08-03 Tissue plasminogen activator
GB878725052A GB8725052D0 (en) 1987-10-26 1987-10-26 Tissue plasminogen activator
GB878726683A GB8726683D0 (en) 1987-11-13 1987-11-13 Tissue plasminogen activator

Publications (1)

Publication Number Publication Date
ES2056082T3 true ES2056082T3 (es) 1994-10-01

Family

ID=27263535

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88112569T Expired - Lifetime ES2056082T3 (es) 1987-08-03 1988-08-02 Nuevo activador de plasminogeno de tipo tisular.

Country Status (11)

Country Link
US (2) US5648250A (es)
EP (1) EP0302456B1 (es)
JP (3) JP2561708B2 (es)
KR (1) KR970001237B1 (es)
AT (1) ATE108206T1 (es)
AU (1) AU623340B2 (es)
CA (1) CA1341458C (es)
DE (1) DE3850531T2 (es)
DK (1) DK174413B1 (es)
ES (1) ES2056082T3 (es)
IL (1) IL87276A (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL87276A (en) * 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of a tissue plasminogen activator containing the Pringle 2 and protease regions only, DNA encoding it, processes for its preparation, and pharmaceutical preparations containing it
IL88247A0 (en) * 1987-11-06 1989-06-30 Genentech Inc Novel human tissue-type plasminogen activator variant
US5094953A (en) * 1988-03-21 1992-03-10 Genentech, Inc. Human tissue plasminogen activator variants
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5676947A (en) * 1989-02-07 1997-10-14 Boehringer Manneheim Gmbh Method for treating thromboembolic conditions using thrombolytically active proteins
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3923339A1 (de) * 1989-05-31 1990-12-06 Boehringer Mannheim Gmbh Gewebs-plasminogen-aktivator-derivat
US5242688A (en) * 1990-12-24 1993-09-07 Eli Lilly And Company Method of treating thromboembolic disorders by administration of diglycosylated t-pa variants
GB9204439D0 (en) 1992-02-27 1992-04-15 Fujisawa Pharmaceutical Co A new cephalosporin c acylase
DE4440892A1 (de) * 1994-11-17 1996-05-23 Gruenenthal Gmbh Proteine mit fibrinolytischen und gerinnungshemmenden Eigenschaften
DE19937219A1 (de) * 1999-08-06 2001-02-08 Aventis Behring Gmbh Verfahren zur Reindarstellung der den Blutgerinnungsfaktor VII aktivierenden Protease, ihres Proenzyms oder eines Gemisches beider Proteine mittels Ionenaustauscherchromatographie
DE19937218A1 (de) * 1999-08-06 2001-02-08 Aventis Behring Gmbh Verfahren zur Reindarstellung der den Blutgerinnungsfaktor VII aktivierenden Protease, ihres Proenzyms oder eines Gemisches beider Proteine mittels Affinitätschromatographie
US6821755B2 (en) * 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
GB0027779D0 (en) * 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods for large scale production of recombinant dna-derived tpa or k2s molecules
US6955910B2 (en) 2000-11-14 2005-10-18 Boehringer Ingelheim International Gmbh Method for large scale production of recombinant DNA-derived TPA or K2S molecules
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
GB0029001D0 (en) * 2000-11-28 2001-01-10 Isis Innovation Assay
DE10153601A1 (de) * 2001-11-02 2003-05-22 Paion Gmbh DSPA zur Behandlung von Schlaganfall
US20080057050A1 (en) * 2003-05-02 2008-03-06 Paion Deutschland Gmbh Intravenous injection of plasminogen non-neurotoxic activators for treating cerebral stroke
AR068914A1 (es) * 2007-10-18 2009-12-16 Paion Deutschland Gmbh Uso de un activador de plasminogeno para la preparacion de un medicamento para el tratamiento de apoplejia
KR100855024B1 (ko) 2008-04-30 2008-08-28 가톨릭대학교 산학협력단 크링글 도메인 １ 및 ２ 로 이루어진 비-글리코실화 재조합단백질
EP2289542A1 (en) * 2009-08-31 2011-03-02 PAION Deutschland GmbH Treatment of neurological or neurodegenerative disorders

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
EP0125254A1 (en) * 1982-10-28 1984-11-21 Beecham Group Plc Enzyme derivatives and their use in the treatment of thrombosis
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
US4577584A (en) * 1984-12-24 1986-03-25 Shih Nan C Bell lock (II)
EP0201153A3 (en) * 1985-02-09 1987-10-07 Beecham Group Plc Modified enzyme and process for its preparation
GB8508717D0 (en) * 1985-04-03 1985-05-09 Beecham Group Plc Composition
EP0199574B1 (en) * 1985-04-22 1991-10-23 Genentech, Inc. Human tissue plasminogen activator mutants, methods and intermediates therefor, and compositions using such mutants
DK43387A (da) * 1986-01-29 1987-07-30 Beecham Group Plc Fibrinolytisk enzym
PT84588B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
US5376547A (en) * 1987-01-30 1994-12-27 American Home Products Corporation Des-epidermal growth factor plasminogen activators
IL87276A (en) * 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of a tissue plasminogen activator containing the Pringle 2 and protease regions only, DNA encoding it, processes for its preparation, and pharmaceutical preparations containing it

Also Published As

Publication number Publication date
DK174413B1 (da) 2003-02-17
CA1341458C (en) 2004-10-12
AU623340B2 (en) 1992-05-14
US5840533A (en) 1998-11-24
JP2561708B2 (ja) 1996-12-11
DE3850531T2 (de) 1994-11-24
KR890003952A (ko) 1989-04-19
IL87276A (en) 1995-07-31
KR970001237B1 (ko) 1997-02-04
JP2606620B2 (ja) 1997-05-07
AU2036188A (en) 1989-02-09
EP0302456B1 (en) 1994-07-06
JP2570633B2 (ja) 1997-01-08
EP0302456A1 (en) 1989-02-08
JPH08228773A (ja) 1996-09-10
DE3850531D1 (de) 1994-08-11
DK431988D0 (da) 1988-08-02
ATE108206T1 (de) 1994-07-15
JPH07163346A (ja) 1995-06-27
IL87276A0 (en) 1988-12-30
JPH01104167A (ja) 1989-04-21
US5648250A (en) 1997-07-15
DK431988A (da) 1989-02-04

Similar Documents

Publication Publication Date Title
ES2056082T3 (es) Nuevo activador de plasminogeno de tipo tisular.
DE69921102D1 (de) Verfahren zur verbesserung der serum halbwertszeit von biologisch aktiven molekülen
HUT58812A (en) Process for producing tissue plasminogen activator (t-pa) zimogenes and compositions containing them as active components
ES554450A0 (es) Un metodo de purificar activador de plasminogeno de tejido humano
CY1737A (en) Desulfatohirudine process for its preparation and pharmaceutical agent
FI925787A0 (fi) Proteolytiska enzymers sackarinderivatinhibitorer
NO302474B1 (no) Sakarinderivater, farmasöytisk sammensetning og anvendelse derav
ES8800347A1 (es) Metodo para preparar uroquinasa de una sola cadena resistente a proteasas
EP0471756A4 (en) Saccharin derivatives useful as proteolytic enzyme inhibitors and preparation thereof
ATE132187T1 (de) Fledermausspeichel-plasminogenaktivator vpa-alpha 1
ES2170747T3 (es) Analogos superiores de trombomodulina para uso farmaceutico.
ATE105862T1 (de) Methoden und materialien zur expression von varianten von menschlichem plasminogen.
SE8702562L (sv) Nya fibrinolytiska enzymer
IL86909A0 (en) Hybrid plasminogen activator,dna coding therefor,derivatives of said plasminogen activator and pharmaceutical compositions containing said plasminogen activator or said derivatives
EP0346741A3 (en) Fibrin(ogen) derivatives, process for preparing them and their use
ES2033295T3 (es) Un metodo para preparar un activador de plasminogeno.
HUT59610A (en) Process for producing pharmaceutical compositions for solving blood clot containing t-pa without glycosilyzing
ES2039194T3 (es) Un metodo de preparar una composicion farmaceutica tromboliticamente activa.
KR890701732A (ko) 개량형 조직 플라스미노겐 활성화 인자
ATE114170T1 (de) Substitutionsvariante des gewebeplasminogenaktivators.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 302456

Country of ref document: ES