ES2016428A6 - Metodo para regenerar y transformar algodon. - Google Patents

Metodo para regenerar y transformar algodon.

Info

Publication number
ES2016428A6
ES2016428A6 ES8803483A ES8803483A ES2016428A6 ES 2016428 A6 ES2016428 A6 ES 2016428A6 ES 8803483 A ES8803483 A ES 8803483A ES 8803483 A ES8803483 A ES 8803483A ES 2016428 A6 ES2016428 A6 ES 2016428A6
Authority
ES
Spain
Prior art keywords
cotton
regeneration
transformation
methods
hypocotyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES8803483A
Other languages
English (en)
Inventor
Thirumale S Rangan
David Maurice Anderson
Kanniah Rajasekaran
John William Grula
Richard Lorne Hudspeth
Richard Lee Yenofsky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phytogen Inc
Original Assignee
Phytogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phytogen Inc filed Critical Phytogen Inc
Publication of ES2016428A6 publication Critical patent/ES2016428A6/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002Culture media for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205Agrobacterium mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223Vegetative tissue-specific promoters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

METODOS PARA REGENERAR Y TRANSFORMAR ALGODON. LA REGENERACION SE EFECTUA CULTIVANDO UNA EXPLANTA DE ALGODON DURANTE UN TIEMPO SUFICIENTE PARA DESARROLLAR CALLOS, TRANSFIRIENDO ESTOS A UN MEDIO DE CRECIMIENTO, CULTIVANDO LOS CALLOS HASTA DESARROLLAR CALLOS EMBRIOGENOS, TRANSFIRIENDO ESTOS A UN MEDIO DE GERMINACION Y CULTIVANDO LOS CALLOS EMBRIOGENOS EN DICHO MEDIO HASTA QUE SE DESARROLLEN PLANTULAS A PARTIR DE ELLOS. LA TRANSFORMACION SE REALIZA TRATANDO UNA EXPLANTA DE ALGODON CON UN VECTOR DE AGROBACTERIUM, INCUBADNO LA EXPLANTA DURANTE 15-200 HORAS A UNA TEMPERATURA DE 25-35GC, TRATANDO LAS EXPLANTAS INCUBADAS CON UN MEDIO QUE CONTIENE UN ANTUBIOTICO CAPAZ DE MATAR LA AGROBACTERIUM, CULTIVANDO LA EXPLANTA LIBRE DE AGROBACTERIUM Y SEPARANDO LOS CALLOS ASI TRANSFORMADOS. LAS PLANTAS DE ALGODON OBTENIDAS SON UTILES POR SU RESISTENCIA A LOS ANTIBIOTICOS NORMALMENTE INHIBIDORES DEL CRECIMIENTO DE SUS CELULAS.
ES8803483A 1987-11-18 1988-11-16 Metodo para regenerar y transformar algodon. Expired - Fee Related ES2016428A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US12220087A 1987-11-18 1987-11-18

Publications (1)

Publication Number Publication Date
ES2016428A6 true ES2016428A6 (es) 1990-11-01

Family

ID=22401286

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8803483A Expired - Fee Related ES2016428A6 (es) 1987-11-18 1988-11-16 Metodo para regenerar y transformar algodon.

Country Status (16)

Country Link
EP (2) EP0344302B1 (es)
JP (2) JPH0822196B2 (es)
KR (3) KR970010757B1 (es)
CN (3) CN1035799C (es)
AT (1) ATE178353T1 (es)
AU (2) AU632038B2 (es)
BR (1) BR8806136A (es)
CA (1) CA1337406C (es)
DE (1) DE3856319T2 (es)
ES (1) ES2016428A6 (es)
GR (1) GR1002421B (es)
IL (1) IL88266A (es)
PT (1) PT89034B (es)
RU (2) RU2128428C1 (es)
WO (1) WO1989005344A1 (es)
ZA (1) ZA888550B (es)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2040174A1 (es) * 1992-02-24 1993-10-01 Consejo Superior Investigacion Metodo para el enraizamiento de estaquillas semilenosas de chirimoyo (anona cherimola mill.) cv. fino de jete, micropropagadas por cultivo de tejidos in vitro.
ES2160019A1 (es) * 1998-09-11 2001-10-16 Consejo Superior Investigacion Procedimiento para la propagacion vegetativa y transformacion genetica de tomate, pimiento y otras dicotiledoneas, mediante agrobacterium tumefaciens.

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317511A3 (de) * 1987-11-18 1991-10-16 Ciba-Geigy Ag Insektizide Baumwollpflanzenzellen
AUPM379294A0 (en) * 1994-02-10 1994-03-03 Commonwealth Scientific And Industrial Research Organisation Expression of the glucose oxidase gene in transgenic organisms
CN1056409C (zh) * 1996-10-09 2000-09-13 中国石化扬子石油化工公司 蝴蝶兰组织培养方法
CN1056410C (zh) * 1996-10-09 2000-09-13 中国石化扬子石油化工公司 一种蝴蝶兰组织培养基组合物
DE69711925D1 (de) 1996-12-06 2002-05-16 Innovations L L C Outdoor Spinnerköder, draht und kabel vorfach zum angeln
JPH11311227A (ja) * 1998-02-25 1999-11-09 Wakai & Co Ltd ビス固定補助具
US6710228B1 (en) 1998-05-29 2004-03-23 Mycogen Corporation Cotton cells, plants, and seeds genetically engineered to express insecticidal and fungicidal chitin binding proteins (lectins)
WO1999061635A2 (en) * 1998-05-29 1999-12-02 Mycogen Corporation Cotton cells, plants, and seeds genetically engineered to express insecticidal and fungicidal chitin binding proteins (lectins)
AU777365B2 (en) * 1999-03-10 2004-10-14 Temasek Life Sciences Laboratory Limited Agrobacterium-mediated transformation of cotton with novel explants
US6483013B1 (en) 1999-05-19 2002-11-19 Bayer Bioscience N.V. Method for agrobacterium mediated transformation of cotton
ATE365218T1 (de) * 1999-05-19 2007-07-15 Bayer Bioscience Nv Verbesserte methode zur agrobakterien- vermittelten transformation von baumwolle
ATE449854T1 (de) * 1999-06-11 2009-12-15 Temasek Life Sciences Lab Ltd Hocheffiziente, durch agrobakterium-vermittelte transformation von baumwoll-pflanzen unter verwendung von blattstielexplantaten
ES2382804T3 (es) 2003-02-12 2012-06-13 Monsanto Technology Llc Evento de algodón MON 88913 y composiciones y procedimientos para su detección
KR100715248B1 (ko) * 2005-10-28 2007-05-09 대한민국 아배양에 의한 개느삼 묘목의 생산방법
BR122017020958B1 (pt) * 2007-05-08 2022-05-10 Monsanto Technology Llc Métodos para melhorar a eficiência da regeneração de planta de algodão
US7858371B2 (en) * 2007-09-26 2010-12-28 Weyerhaeuser Nr Company Method of separating embryo suspension mass
CN102405835A (zh) * 2011-08-23 2012-04-11 中国农业大学 一种高效诱导棉花体胚发生与植株再生的方法及其培养基
CN102577980A (zh) * 2012-03-16 2012-07-18 甘肃省农业科学院生物技术研究所 彩棉体细胞胚状体的萌发及成苗方法
AU2020277499A1 (en) * 2019-05-23 2021-12-23 Galy Co. Compositions and methods for plant cell culture
CN115380721A (zh) * 2022-10-09 2022-11-25 湖北省农业科学院经济作物研究所 一种高效的棉花转基因植株嫁接技术

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4672035A (en) * 1984-03-16 1987-06-09 Research Corporation Controlled regeneration of cotton plants from tissue culture
EP0207965A4 (en) * 1984-12-13 1987-06-17 Bioteknika International VECTOR FOR PLANT TRANSFORMATION.
AU590597B2 (en) * 1985-08-07 1989-11-09 Monsanto Technology Llc Glyphosate-resistant plants
EP0243469A4 (en) * 1985-10-22 1988-02-16 Plant Genetics Inc IMPROVED SOMATIC ENBRYOGENESIS METHODS AND AGENTS.
US5004863B2 (en) * 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
TR27832A (tr) * 1987-04-29 1995-08-31 Monsanto Co Zararli ucucu hasarata mukavim bitkiler.
EP0317512B1 (de) * 1987-11-18 1992-08-05 Ciba-Geigy Ag Eine leistungsfähige Methode, Baumwolle aus kultivierten Zellen zu regenerieren
EP0317511A3 (de) * 1987-11-18 1991-10-16 Ciba-Geigy Ag Insektizide Baumwollpflanzenzellen

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2040174A1 (es) * 1992-02-24 1993-10-01 Consejo Superior Investigacion Metodo para el enraizamiento de estaquillas semilenosas de chirimoyo (anona cherimola mill.) cv. fino de jete, micropropagadas por cultivo de tejidos in vitro.
ES2160019A1 (es) * 1998-09-11 2001-10-16 Consejo Superior Investigacion Procedimiento para la propagacion vegetativa y transformacion genetica de tomate, pimiento y otras dicotiledoneas, mediante agrobacterium tumefaciens.

Also Published As

Publication number Publication date
CN1035799C (zh) 1997-09-10
CN1032111C (zh) 1996-06-26
DE3856319T2 (de) 1999-07-15
AU668915B2 (en) 1996-05-23
PT89034B (pt) 1994-08-31
RU2128428C1 (ru) 1999-04-10
AU632038B2 (en) 1992-12-17
BR8806136A (pt) 1989-08-15
AU3528493A (en) 1993-05-20
EP0344302A1 (en) 1989-12-06
GR1002421B (el) 1996-08-22
KR100303729B1 (ko) 2001-12-17
IL88266A (en) 1998-03-10
ZA888550B (en) 1989-08-30
KR100198008B1 (en) 1999-06-15
JPH02502253A (ja) 1990-07-26
KR970010757B1 (ko) 1997-06-30
AU2926689A (en) 1989-07-05
CA1337406C (en) 1995-10-24
IL88266A0 (en) 1989-06-30
EP0344302A4 (en) 1991-09-25
GR880100761A (el) 1994-03-31
CN1034298A (zh) 1989-08-02
ATE178353T1 (de) 1999-04-15
JPH0822196B2 (ja) 1996-03-06
CN1070310A (zh) 1993-03-31
EP0899341A3 (en) 1999-04-21
JPH084434B2 (ja) 1996-01-24
EP0344302B1 (en) 1999-03-31
JPH0765A (ja) 1995-01-06
DE3856319D1 (de) 1999-05-06
CN1032401C (zh) 1996-07-31
KR890701729A (ko) 1989-12-21
WO1989005344A1 (en) 1989-06-15
CN1070309A (zh) 1993-03-31
PT89034A (pt) 1989-11-30
RU2225882C2 (ru) 2004-03-20
EP0899341A2 (en) 1999-03-03

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SA6 Expiration date (snapshot 920101)

Free format text: 2008-11-16

PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20081117