ES2012323T3 - Marcadores quimioluminiscentes protegidos. - Google Patents
Marcadores quimioluminiscentes protegidos.Info
- Publication number
- ES2012323T3 ES2012323T3 ES89301679T ES89301679T ES2012323T3 ES 2012323 T3 ES2012323 T3 ES 2012323T3 ES 89301679 T ES89301679 T ES 89301679T ES 89301679 T ES89301679 T ES 89301679T ES 2012323 T3 ES2012323 T3 ES 2012323T3
- Authority
- ES
- Spain
- Prior art keywords
- label
- protected
- linked
- specific binding
- binding partner
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Heterocyclic Compounds That Contain Two Or More Ring Oxygen Atoms (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Details Of Garments (AREA)
- Luminescent Compositions (AREA)
Abstract
UN METODO PARA PREPARAR UNA PAREJA ENLAZANTE ESPECIFICA MARCADA, TAL COMO UNA SONDA ESPECIFICA, EN FORMA DE UNA SONDA DE ANTICUERPO U OLIGONUCLEOTIDO UTILIZANDO UN MARCADOR PROTEGIDO, SIENDO SUSCEPTIBLE DE INACTIVACION EL MARCADOR NO PROTEGIDO CORRESPONDIENTE, TAL COMO POR HIDROLISIS, PARA PRODUCIR UNA FORMA NO QUIMILUMINISCENTE DEL MARCADOR. LA PAREJA ENLAZANTE ESPECIFICA SE UNE AL MARCADOR Y SE PREPARA UN ADUCTO DEL MARCADOR UTILIZANDO UN PRIMER ADUCTO PROTECTOR, QUE PRODUCE UN MARCADOR PROTEGIDO, QUE ES MENOS SUSCEPTIBLE A LA INACTIVACION. MARCADORES ESPECIALMENTE PREFERIDOS SON LOS ACRIDINIOS Y ACRIDANOS, MAS PREFERIBLEMENTE LOS PRIMEROS, QUE TIENEN LA ESTRUCTURA GENERAL: (FIG. A), DONDE LOS ANILLOS FENILICOS ESTAN SUSTITUIDOS ADICIONALMENTE DE MODO OPCIONAL, R1 ES PREFERIBLEMENTE UN ALQUILO, Y R5 ES OPCIONALMENTE UN HIDROCARBURO SUSTITUIDO, MAS PREFERIBLEMENTE UNA PARTE FENILICA QUE, O BIEN ESTA UNIDA A LA PAREJA ENLAZANTE ESPECIFICA, O ES CAPAZ DE ESTAR UNIDA A ELLA. LA FORMACION DELMARCADOR PROTEGIDO ES PREFERIBLEMENTE UNA REACCION EN EQUILIBRIO, QUE ES FACILMENTE REVERSIBLE, TAL COMO POR DILUCION U OXIDACION DEL PRIMER ADUCTO PROTECTOR. TAMBIEN SE DESCRIBEN MARCADORES UTILES EN TAL METODO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/160,611 US4950613A (en) | 1988-02-26 | 1988-02-26 | Proteted chemiluminescent labels |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2012323A4 ES2012323A4 (es) | 1990-03-16 |
ES2012323T3 true ES2012323T3 (es) | 1996-06-16 |
Family
ID=22577607
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES89301679T Expired - Lifetime ES2012323T3 (es) | 1988-02-26 | 1989-02-22 | Marcadores quimioluminiscentes protegidos. |
Country Status (15)
Country | Link |
---|---|
US (1) | US4950613A (es) |
EP (1) | EP0330433B1 (es) |
JP (1) | JPH02503268A (es) |
KR (1) | KR0132290B1 (es) |
AT (1) | ATE137021T1 (es) |
AU (1) | AU626319B2 (es) |
CA (1) | CA1333396C (es) |
DE (2) | DE68926259T2 (es) |
DK (1) | DK530489A (es) |
ES (1) | ES2012323T3 (es) |
FI (1) | FI895062A0 (es) |
GR (1) | GR900300054T1 (es) |
NO (1) | NO894218L (es) |
PT (1) | PT89834B (es) |
WO (1) | WO1989008256A1 (es) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6414152B1 (en) * | 1981-12-11 | 2002-07-02 | University Of Wales College Of Medicine Of Heath Park | Luminescent labelling material and procedures |
US5605795A (en) * | 1986-07-24 | 1997-02-25 | Tropix, Inc. | Assays using chemiluminescent, enzymatically cleavable substituted 1,2-dioxetanes and kits therefor |
US5110932A (en) * | 1986-10-06 | 1992-05-05 | Ciba Corning Diagnostics Corp. | Polysubstituted aryl acridinium esters |
US5338847A (en) * | 1987-12-31 | 1994-08-16 | London Diagnostics, Inc. | Hydrolytically stable chemiluminescent labels and their conjugates, and assays therefrom by adduct formation |
US5284952A (en) * | 1987-12-31 | 1994-02-08 | London Diagnostics, Inc. | Sulfonyl substituted chemiluminescent labels and their conjugates, and assays therefrom |
US5321136A (en) * | 1987-12-31 | 1994-06-14 | London Diagnostics, Inc. | Peri substituted fused ring chemiluminescent labels and their conjugates, and assays therefrom |
US5227489A (en) * | 1988-08-01 | 1993-07-13 | Ciba Corning Diagnostics Corp. | Stable hydrophilic acridinium esters suitable for liposome encapsulation |
US5663074A (en) * | 1988-09-26 | 1997-09-02 | Chiron Diagnostics Corporation | Nucleophilic polysubstituted aryl acridinium ester conjugates and syntheses thereof |
US5241070A (en) * | 1988-09-26 | 1993-08-31 | Ciba Corning Diagnostics Corp. | Nucleophilic polysubstituted aryl acridinium esters and uses thereof |
US5629168A (en) * | 1992-02-10 | 1997-05-13 | British Technology Group Limited | Chemiluminescent enhancers |
WO1993020074A1 (en) * | 1992-03-27 | 1993-10-14 | Abbott Laboratories | Haptens, tracers, immunogens and antibodies for acridine-9-carboxylic acids |
JPH07509245A (ja) * | 1992-07-20 | 1995-10-12 | デイド・ベーリング・マルブルク・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | 新規化学発光化合物およびその使用方法 |
US6002000A (en) * | 1992-07-20 | 1999-12-14 | Dade Behring Marburg Gmbh | Chemiluminescent compounds and methods of use |
JP3584379B2 (ja) * | 1993-02-04 | 2004-11-04 | 持田製薬株式会社 | アクリジニウム化合物およびアクリジニウム化合物複合体 |
US5670644A (en) * | 1993-05-17 | 1997-09-23 | Lumigen, Inc. | Acridan compounds |
JPH08233820A (ja) * | 1995-02-23 | 1996-09-13 | Kdk Corp | 化学発光測定法 |
US5731148A (en) * | 1995-06-07 | 1998-03-24 | Gen-Probe Incorporated | Adduct protection assay |
DE69711188T2 (de) * | 1996-07-16 | 2002-09-05 | Nederlanden Staat | Dibenzodihydropyridincarbonsäureester und ihre anwendung in chemolumineszenz-untersuchungsverfahren |
AU7487798A (en) * | 1997-05-29 | 1998-12-30 | Bio-Rad Laboratories, Inc. | Chemiluminescent hemoglobin assay |
ATE423622T1 (de) | 1998-05-01 | 2009-03-15 | Gen Probe Inc | Automatisches isolierungs- und amplifizierungsverfahren für eine zielnukleinsäuresequenz |
AU2012232988B2 (en) * | 1998-05-01 | 2014-09-25 | Gen-Probe Incorporated | System and method for incubating the contents of a reaction receptacle |
WO2000020643A1 (en) | 1998-10-05 | 2000-04-13 | Mosaic Technologies | Reverse displacement assay for detection of nucleic acid sequences |
US6673560B1 (en) * | 1998-11-25 | 2004-01-06 | Bayer Corporation | Measurement of hydride using chemiluminescent acridinium compounds and applications thereof |
CA2362545C (en) | 1999-02-12 | 2007-09-25 | Gen-Probe Incorporated | Protection probes |
US6127140A (en) * | 1999-06-18 | 2000-10-03 | Abbott Laboratories | Assay for quantitative measurement of analytes in biological samples |
US6723851B2 (en) | 2001-10-31 | 2004-04-20 | Quest Diagnostics Investment Incorporated | Chemiluminescent compounds and use thereof |
WO2003097808A2 (en) * | 2002-05-17 | 2003-11-27 | Becton, Dickinson And Company | Automated system for isolating, amplyifying and detecting a target nucleic acid sequence |
AU2004235747B2 (en) | 2003-05-01 | 2009-05-28 | Gen-Probe Incorporated | Oligonucleotides comprising a molecular switch |
US20080318226A1 (en) * | 2005-02-28 | 2008-12-25 | Eisair & Dmanagement Co., Ltd. | Signal Amplification Method |
EP1909108B1 (en) | 2005-03-10 | 2019-05-29 | Gen-Probe Incorporated | Systems and methods to perform assays for detecting or quantifiying analytes |
EP2322940B1 (en) | 2005-03-10 | 2014-10-29 | Gen-Probe Incorporated | Systems amd methods to perform assays for detecting or quantifing analytes within samples |
EP2752671A3 (en) | 2010-07-23 | 2016-08-24 | Beckman Coulter, Inc. | System or method of including analytical units |
CN104040352B (zh) | 2011-11-07 | 2016-09-07 | 贝克曼考尔特公司 | 机械臂 |
EP2776844B1 (en) | 2011-11-07 | 2020-09-30 | Beckman Coulter, Inc. | Specimen container detection |
JP2014532881A (ja) | 2011-11-07 | 2014-12-08 | ベックマン コールター, インコーポレイテッド | 標本輸送システムのための磁気制動 |
EP2776843B1 (en) | 2011-11-07 | 2019-03-27 | Beckman Coulter, Inc. | Centrifuge system and workflow |
JP6190380B2 (ja) | 2011-11-07 | 2017-08-30 | ベックマン コールター, インコーポレイテッド | 等分機システムおよびワークフロー |
BR112014010955A2 (pt) | 2011-11-07 | 2017-06-06 | Beckman Coulter Inc | sistema e método para processar amostras |
US10427162B2 (en) | 2016-12-21 | 2019-10-01 | Quandx Inc. | Systems and methods for molecular diagnostics |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU6771981A (en) * | 1979-12-19 | 1981-07-22 | Electro-Nucleonics Inc. | Chemical luminescence amplification substrate system for immuno chemistry |
US4372745A (en) * | 1979-12-19 | 1983-02-08 | Electro-Nucleonics, Inc. | Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer |
EP0082636B2 (en) * | 1981-12-11 | 2006-10-18 | The Welsh National School of Medicine | Luminescent labelling materials and procedures |
DE3645292C2 (de) * | 1986-08-22 | 1997-12-11 | Hoechst Ag | Chemilumineszenzimmunoassays und ein Verfahren zu ihrer Herstellung |
US4745181A (en) * | 1986-10-06 | 1988-05-17 | Ciba Corning Diagnostics Corp. | Polysubstituted aryl acridinium esters |
US4927769A (en) * | 1987-07-08 | 1990-05-22 | Ciba Corning Diagnostics Corp. | Method for enhancement of chemiluminescence |
ES2074051T3 (es) * | 1987-09-21 | 1995-09-01 | Gen Probe Inc | Ensayo de proteccion homogeneo. |
NZ227506A (en) * | 1987-12-31 | 1992-06-25 | London Diagnostics Inc | Specific binding assays using chemiluminescent compounds |
-
1988
- 1988-02-26 US US07/160,611 patent/US4950613A/en not_active Expired - Lifetime
-
1989
- 1989-02-22 EP EP89301679A patent/EP0330433B1/en not_active Expired - Lifetime
- 1989-02-22 AT AT89301679T patent/ATE137021T1/de not_active IP Right Cessation
- 1989-02-22 ES ES89301679T patent/ES2012323T3/es not_active Expired - Lifetime
- 1989-02-22 DE DE68926259T patent/DE68926259T2/de not_active Expired - Fee Related
- 1989-02-22 DE DE198989301679T patent/DE330433T1/de active Pending
- 1989-02-24 CA CA000591999A patent/CA1333396C/en not_active Expired - Fee Related
- 1989-02-24 PT PT89834A patent/PT89834B/pt active IP Right Grant
- 1989-02-27 WO PCT/US1989/000722 patent/WO1989008256A1/en active Application Filing
- 1989-02-27 JP JP1503114A patent/JPH02503268A/ja active Pending
- 1989-02-27 AU AU40723/89A patent/AU626319B2/en not_active Expired
- 1989-02-27 KR KR1019890701986A patent/KR0132290B1/ko not_active IP Right Cessation
- 1989-10-24 NO NO89894218A patent/NO894218L/no unknown
- 1989-10-25 DK DK530489A patent/DK530489A/da not_active Application Discontinuation
- 1989-10-25 FI FI895062A patent/FI895062A0/fi not_active IP Right Cessation
-
1991
- 1991-07-31 GR GR90300054T patent/GR900300054T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
ATE137021T1 (de) | 1996-05-15 |
DE330433T1 (de) | 1990-05-03 |
PT89834B (pt) | 1994-02-28 |
DK530489A (da) | 1989-12-27 |
CA1333396C (en) | 1994-12-06 |
US4950613A (en) | 1990-08-21 |
ES2012323A4 (es) | 1990-03-16 |
PT89834A (pt) | 1989-10-04 |
JPH02503268A (ja) | 1990-10-11 |
WO1989008256A1 (en) | 1989-09-08 |
EP0330433A3 (en) | 1991-02-27 |
KR900700885A (ko) | 1990-08-17 |
DE68926259D1 (de) | 1996-05-23 |
FI895062A0 (fi) | 1989-10-25 |
DE68926259T2 (de) | 1996-09-19 |
EP0330433B1 (en) | 1996-04-17 |
NO894218L (no) | 1989-12-22 |
NO894218D0 (no) | 1989-10-24 |
DK530489D0 (da) | 1989-10-25 |
KR0132290B1 (ko) | 1998-04-11 |
EP0330433A2 (en) | 1989-08-30 |
GR900300054T1 (en) | 1991-07-31 |
AU4072389A (en) | 1989-09-22 |
AU626319B2 (en) | 1992-07-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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