EP3562833A4 - ALGAE MUTANTS WITH HIGH PRODUCTIVITY AND REDUCED PHOTOSYNTHETIC ANTENNA - Google Patents

ALGAE MUTANTS WITH HIGH PRODUCTIVITY AND REDUCED PHOTOSYNTHETIC ANTENNA Download PDF

Info

Publication number
EP3562833A4
EP3562833A4 EP17887143.0A EP17887143A EP3562833A4 EP 3562833 A4 EP3562833 A4 EP 3562833A4 EP 17887143 A EP17887143 A EP 17887143A EP 3562833 A4 EP3562833 A4 EP 3562833A4
Authority
EP
European Patent Office
Prior art keywords
high productivity
algal mutants
reduced photosynthetic
photosynthetic antenna
antenna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP17887143.0A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP3562833A1 (en
Inventor
Eric R. MOELLERING
Nicholas BAUMAN
Randor R. RADAKOVITS
Roberto SPREAFICO
Fedor KUZMINOV
Imad AJJAWI
Saheed IMAM
Andrew Schultz
Kathleen KWOK
Moena AQUI
Jennifer NOMINATI
John Verruto
Shaun Bailey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Viridos Inc
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of EP3562833A1 publication Critical patent/EP3562833A1/en
Publication of EP3562833A4 publication Critical patent/EP3562833A4/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C12N1/125—Unicellular algae isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8269—Photosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Physiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
EP17887143.0A 2016-12-30 2017-12-29 ALGAE MUTANTS WITH HIGH PRODUCTIVITY AND REDUCED PHOTOSYNTHETIC ANTENNA Pending EP3562833A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662441002P 2016-12-30 2016-12-30
PCT/US2017/069073 WO2018126201A1 (en) 2016-12-30 2017-12-29 High productivity algal mutants having reduced photosynthetic antenna

Publications (2)

Publication Number Publication Date
EP3562833A1 EP3562833A1 (en) 2019-11-06
EP3562833A4 true EP3562833A4 (en) 2020-07-01

Family

ID=62708892

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17887143.0A Pending EP3562833A4 (en) 2016-12-30 2017-12-29 ALGAE MUTANTS WITH HIGH PRODUCTIVITY AND REDUCED PHOTOSYNTHETIC ANTENNA

Country Status (9)

Country Link
US (3) US10968259B2 (ja)
EP (1) EP3562833A4 (ja)
JP (1) JP7057786B2 (ja)
AU (1) AU2017388870B2 (ja)
BR (1) BR112019013193A2 (ja)
CA (1) CA3047811A1 (ja)
MX (1) MX2019007765A (ja)
SA (1) SA519401668B1 (ja)
WO (1) WO2018126201A1 (ja)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201704272YA (en) * 2014-12-31 2017-06-29 Synthetic Genomics Inc Compositions and methods for high efficiency in vivo genome editing
US11193132B2 (en) * 2017-12-29 2021-12-07 Synthetic Genomics, Inc. Genetic modulation of photosynthetic organisms for improved growth
CN112384619A (zh) * 2018-07-23 2021-02-19 合成基因组股份有限公司 具有增加的脂质产生的微生物和组合物及其制备和使用方法
US20220010321A1 (en) * 2018-11-01 2022-01-13 Keygene N.V. Dual guide rna for crispr/cas genome editing in plants cells
JP7397437B2 (ja) * 2018-11-07 2023-12-13 学校法人 中央大学 クロロフィル含有率が減少し、且つ油脂生産性が増加した緑藻変異体及びその利用
WO2020111028A1 (ja) * 2018-11-26 2020-06-04 株式会社カネカ ゲノム編集方法
JP7416384B2 (ja) * 2018-11-26 2024-01-17 株式会社カネカ ゲノム編集方法
CN110033825B (zh) * 2019-04-26 2021-08-13 扬州大学 一种箭筈豌豆种质资源筛选方法
MX2022007430A (es) * 2019-12-17 2022-09-12 Viridos Inc Algas recombinantes con alta productividad de lipidos.
CN116323646A (zh) * 2020-09-11 2023-06-23 维里多斯公司 具有高脂质生产力的重组藻类
WO2022080234A1 (ja) * 2020-10-12 2022-04-21 Dic株式会社 ガルデリア属に属する藻類のゲノム改変方法
US20220348946A1 (en) * 2021-04-23 2022-11-03 Viridos, Inc. Chlorophyte algae having improved productivity
WO2024159138A1 (en) * 2023-01-27 2024-08-02 Gentibio, Inc. Engineered repair regulatory t cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014089533A2 (en) * 2012-12-06 2014-06-12 Synthetic Genomics, Inc. Algal mutants having a locked-in high light acclimated phenotype

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002099079A2 (en) 2001-06-06 2002-12-12 The General Hospital Corporation Cytokinin response regulators and uses thereof
WO2016168756A1 (en) 2015-04-15 2016-10-20 Synthetic Genomics, Inc. Algal chloroplastic srp54 mutants

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014089533A2 (en) * 2012-12-06 2014-06-12 Synthetic Genomics, Inc. Algal mutants having a locked-in high light acclimated phenotype

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DE MOOIJ TIM ET AL: "Modeling the competition between antenna size mutant and wild type microalgae in outdoor mass culture", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 240, 13 October 2016 (2016-10-13), pages 1 - 13, XP029808993, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2016.10.009 *
HENNING KIRST ET AL: "The chloroplast signal recognition particle (CpSRP) pathway as a tool to minimize chlorophyll antenna size and maximize photosynthetic productivity", BIOTECHNOLOGY ADVANCES., vol. 32, no. 1, 28 February 2014 (2014-02-28), GB, pages 66 - 72, XP055321704, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2013.08.018 *

Also Published As

Publication number Publication date
AU2017388870A1 (en) 2019-07-04
US20180186842A1 (en) 2018-07-05
CA3047811A1 (en) 2018-07-05
MX2019007765A (es) 2019-09-09
JP2020503051A (ja) 2020-01-30
US20210188924A1 (en) 2021-06-24
AU2017388870B2 (en) 2023-01-12
SA519401668B1 (ar) 2023-03-09
WO2018126201A1 (en) 2018-07-05
US20240052001A1 (en) 2024-02-15
EP3562833A1 (en) 2019-11-06
JP7057786B2 (ja) 2022-04-20
BR112019013193A2 (pt) 2019-12-10
US11787843B2 (en) 2023-10-17
US10968259B2 (en) 2021-04-06

Similar Documents

Publication Publication Date Title
EP3562833A4 (en) ALGAE MUTANTS WITH HIGH PRODUCTIVITY AND REDUCED PHOTOSYNTHETIC ANTENNA
EP3587557A4 (en) LARVE DETENEBRIO ISOLATED MICRO-ORGANISM
EP3555282A4 (en) ENZYMATIC PRODUCTION OF D-ALLULOSE
EP3303594A4 (en) Genetically engineered microorganisms for the production of chorismate-derived products
EP3728614A4 (en) MICROORGANISMS AND METHODS FOR THE BIOLOGICAL MANUFACTURING OF EHTYLENE GLYCOL
EP3371303A4 (en) ALGAE MUTANTS WITH INCREASED LIPID PRODUCTIVITY
EP3510140A4 (en) NEW CULTURE SYSTEM FOR EFFICIENT PRODUCTION OF MICROORGANISMS
EP3856888A4 (en) THREE-DIMENSIONAL BIOREACTORS
EP3365107B8 (en) Cell culture
EP3504315A4 (en) BIOREACTOR
EP3674394A4 (en) PRIMARY CULTURE PROCESS
EP3501035A4 (en) PROCESS OPTIMIZATION USING A DOUBLE-SIDED EPITAXIAL LAYER ON A SUBSTRATE
EP3993798A4 (en) NOVEL PROCESSES
EP3344776A4 (en) MODIFIED MICROORGANISMS TO IMPROVE PRODUCTIVITY
EP3322799A4 (en) MICROORGANISMS WITH INCREASED LIPID PRODUCTIVITY
EP3508566A4 (en) METHOD FOR CULTURING PHOTOSYNTHETIC MICROALGAE
EP3283616A4 (en) Genetically engineered c1-utilizing microorganisms and processes for their production and use
EP3443110A4 (en) FERMENTATIVE METHOD FOR THE PRODUCTION OF MALTOSYL ISOMALTOOLIGOSACCHARIDES (MIMO)
EP3283090A4 (en) Algal chloroplastic srp54 mutants
EP4034119A4 (en) NEW METHODS
EP3448986A4 (en) CELL CULTURE
EP3541933A4 (en) TUMOR EPITHELIAL CELL CULTURES
EP3587571A4 (en) NEW MICRO-ORGANISM AND METHOD OF PRODUCTION OF UROLITHINS USING IT
EP3526234A4 (en) PRODUCTION OF RECOMBINANT MICROCYSTINE
EP3448816A4 (en) FLOATING BIOREACTOR

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20190729

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20200529

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 1/13 20060101ALI20200525BHEP

Ipc: C07H 21/04 20060101AFI20200525BHEP

Ipc: C12N 1/12 20060101ALI20200525BHEP