EP3335795B1 - Procédé de dosage de liquide au moyen d'une pipette et d'une seringue et pipette destinée à actionner une seringue pour le dosage de liquides - Google Patents

Procédé de dosage de liquide au moyen d'une pipette et d'une seringue et pipette destinée à actionner une seringue pour le dosage de liquides Download PDF

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Publication number
EP3335795B1
EP3335795B1 EP16204865.6A EP16204865A EP3335795B1 EP 3335795 B1 EP3335795 B1 EP 3335795B1 EP 16204865 A EP16204865 A EP 16204865A EP 3335795 B1 EP3335795 B1 EP 3335795B1
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EP
European Patent Office
Prior art keywords
syringe
dosing
pipette
steps
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
EP16204865.6A
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German (de)
English (en)
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EP3335795A1 (fr
Inventor
Burkhardt Reichmuth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
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Eppendorf SE
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Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to EP16204865.6A priority Critical patent/EP3335795B1/fr
Priority to PL16204865T priority patent/PL3335795T3/pl
Priority to CN201711349275.6A priority patent/CN108201903B/zh
Priority to JP2017241011A priority patent/JP6639461B2/ja
Priority to US15/844,921 priority patent/US11154854B2/en
Publication of EP3335795A1 publication Critical patent/EP3335795A1/fr
Application granted granted Critical
Publication of EP3335795B1 publication Critical patent/EP3335795B1/fr
Priority to US16/934,243 priority patent/US11697113B2/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0234—Repeating pipettes, i.e. for dispensing multiple doses from a single charge
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/027—Digital display, e.g. LCD, LED
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0237—Details of electronic control, e.g. relating to user interface

Definitions

  • the invention relates to a method for dosing liquid by means of a pipette and a syringe and a pipette for actuating a syringe for dosing liquid.
  • the pipettes used here for actuating a syringe serve to dispense liquid received in the syringe in several steps. They are also referred to as dispensers or repeating pipettes. These pipettes have at the lower end of a rod-shaped housing a receptacle for a flange of a cylinder of the syringe and in the housing a displaceable receiving body with a piston receptacle for the upper end portion of a piston rod of a piston of the syringe.
  • the syringe can be used with the flange and the end portion of the piston rod through axially directed openings of the receptacles.
  • the flange and the end portion are held in the receptacles by means for detachable holding, which are designed, for example, as spring-loaded gripping levers.
  • the pipette comprises means for displacing the receiving body, which allow a partial withdrawal of the piston from the cylinder for the suction of liquid into the syringe and a stepwise depression of the piston into the cylinder for the stepwise discharge of liquid.
  • the DE 29 26 691 C2 and the US 4,406,170 A1 describe means for displacing the receiving body in the housing. These include an elevator lever connected to the receiving body and projecting out of the housing through a rectilinear slot for drawing fluid into the syringe by displacing the receiving body away from the receptacle. In addition, they include a rack-pawl means for gradually advancing the piston by a reciprocating metering lever. At the metering a pivoting pawl is mounted. The rack is connected to the receiving body and in the swivel range arranged the latch. An adjustable sliding cover covers more or less the row of teeth on the rack in order to limit the engagement of the pawl in the rack when pivoting the metering lever.
  • the rack is designed with a contour through which in the advanced position of the piston, the cover of the rack is displaced away, so that it prevents the pawl from engaging in the uncovered teeth of the rack. This prevents that a residual amount is discharged from the syringe, which is smaller than the dispensed in each dosing step dosage.
  • the EP 0 657 216 B1 and US 5,620,661A describe such a pipette with a sensor for sensing protrusions and depressions on the syringe flange and associated syringes.
  • the sensor is used to determine the size of the syringe used.
  • electronics determines the amount of fluid dispensed at each dispensing step. This is displayed on a display.
  • a Repetierpipette has a Konstant Colourinraum, which sets the width of the first step for moving the receiving body for the operating portion of the syringe plunger for cylinder intake for the syringe barrel to a constant value, which is independent of the setting of the subsequent step sizes.
  • the EP 2 656 916 A1 and the US 2014/0010731 A1 describe a method for dosing liquid by means of a pipette and a syringe and a pipette for actuating a syringe for dosing liquid.
  • the maximum number of metering steps possible with the set metering step width and completely filled syringe without refilling the syringe is indicated by means of a display device of the pipette. After the reversal stroke, the metering steps performed are counted and / or the number of metering steps still possible without refilling the syringe is determined and displayed by means of the display device.
  • the dosing steps performed in total with the syringe with the set dosing increment are counted and / or the number of dosing steps still possible without refilling the syringe is determined and displayed. If the syringe is detached from the pipette and replaced with a new syringe, the dosing steps performed are counted from the beginning and / or the dosing steps still possible without refilling are determined and displayed.
  • the display device of the pipette displays an indication of the execution of a reverse stroke until the reverse stroke is performed by one or more dosing steps and the subsequent dosing steps are counted and displayed by means of the display device.
  • the user is indicated by a flashing display that the reaching of the indicated maximum number of dosing steps at full filling is not ensured.
  • the flashing is only ended when the user has performed a sufficient number of dosing steps that correspond to a reversal stroke. This indicates to the user that no accurate dosing has occurred and he should discard the dispensed doses.
  • the control device indicates via the display device the set metering volume and the number of metering steps that have taken place. However, the display continues to count after the Resthubsperre has been inserted so that the user does not know how many metering steps with the correct volume have actually been dispensed.
  • the object of the invention is to provide a method for dosing liquid by means of a pipette and a syringe and a pipette for actuating a syringe for dosing liquid, which allows the user to display even when the syringe is partially filled to ensure the maximum possible number of dosing steps.
  • the maximum number of metering steps possible without refilling the syringe is determined with the aid of the detected position of the drive element on the web at the end of the filling of the syringe.
  • the web is rectilinear in the execution of the drive element as a rack.
  • the reversal stroke to be executed is preferably carried out by performing one or more dosing by at least one time pressing the control knob.
  • the number of actuations of the operating knob for the execution of the reverse stroke depends on the set dosing increment.
  • An actuation of the control knob is understood to be a complete displacement of the control knob from a defined initial position to a defined end position, which are defined, for example, by stops or increasing resistances against further displacement beyond the respective initial or end position.
  • the respective position of a drive element is detected during the displacement along a path by means of an incremental encoder arranged on a displacement element of a means for displacing a piston in a cylinder of the syringe and at least one sensor fixedly arranged in a housing of the pipette with respect to the displacement element
  • the incremental encoder is an optical scale or magnetic strip and the sensor is a light barrier or a magnetic sensor.
  • the position of the drive element is detected by means of a capacitive sensor having at least one arranged on a displacement element of the means for displacing the first capacitor plate and at least one fixedly arranged in the housing, the second capacitor plate.
  • the displacement element is a rack and the incremental encoder formed integrally with the rack or a fixed to the rack connected component.
  • the incremental encoder is formed by the toothing of the toothed rack.
  • the incremental encoder is glued to the displacement element, injected into this or printed on this.
  • the position of the drive element is detected by means of a rotary encoder, which is coupled via a gear transmission or another gear with the displacement element.
  • the maximum number of metering steps possible without refilling the syringe is determined as in the case of partial filling of the syringe.
  • the reverse stroke can be carried out as in partial filling by pressing the dosing button one or more times, depending on the dosing increment set.
  • the displacement of the drive element and the reversal stroke to be executed are known. Therefore, according to a further embodiment, the maximum number of metering steps possible without refilling the syringe with the aid of the set metering step width, the known displacement of the drive element determined with complete filling of the syringe and the reversing stroke to be carried out.
  • the displacement of the drive element when completely filled is defined, for example, by an initial position and an end position between which the drive element is displaced along a path when completely filled.
  • the reversal stroke is carried out by means of a constant incremental device according to the prior art mentioned in the introduction.
  • the reversal stroke is performed with a fully and partially filled syringe by means of a constant-pitch device, which is effective when fully and partially filled syringe.
  • the user can carry out dosing steps by pressing the operating button.
  • the dosing steps performed are counted and the number of dosing steps performed and / or the number of dosing steps still possible without refilling the syringe are determined and displayed by means of the display device.
  • the further work with the same syringe or a new syringe after performing the possible without refilling the syringe maximum number of metering steps corresponds to the already known method.
  • the number of actuations of the control knob for the reversal stroke to be performed is determined for the set dosing increment and the pipette outputs an indication and / or a signal and / or another output indicative of the execution of the required number of actuations until the reversing stroke to be performed is performed and / or when the reversing stroke to be performed is executed.
  • This instructs the user to operate the control knob until complete execution of the reversing stroke to be performed.
  • the output can be made until the reversing stroke to be executed is executed or only when the reversing stroke to be executed is executed.
  • Combinations are also possible, and preferably before the execution of the reversing stroke to be executed, a different output than after Execution of the executed Umledgehubs done.
  • the user can look in a table at which set dosing step size which number of actuations of the control knob is required so that the reversal stroke to be executed is performed.
  • the maximum number of metering steps possible with the set metering step width and completely filled syringe after performing the reversing stroke without refilling the syringe is indicated by means of the display device. If the user completely fills the syringe, he can perform these indicated maximum possible number of dosing steps without refilling the syringe. If the user stops filling before filling the syringe completely, the maximum number of dosing steps possible with the syringe partially filled without refilling the syringe will be displayed.
  • the user has the option of refilling the syringe if the possible maximum number of metering steps indicated on partial filling is insufficient.
  • the user can only perform the maximum number of metering steps possible with partial filling without refilling the syringe or empty the syringe by means of the extraction lever and then refill it.
  • the completion of the filling of the syringe is recognized by the fact that the displacement of the drive element is stopped and / or the direction of the displacement of the drive element is reversed.
  • in the first variant further filling of the syringe without prior emptying is possible and in the second variant no further filling of the syringe without prior emptying of the syringe is possible.
  • the display shows the maximum with the newly set Dosier intimide without refilling the syringe number of dosing and the new set dosing increment set dosing volume. This allows more flexible dosing.
  • the display of the maximum possible number of metering steps is possible without refilling the syringe.
  • the pipette detects a syringe volume indicating coding of the syringe connected to the pipette and determines the dosing volume due to the set dosing increment and the detected coding and displays the dosing volume by means of the display device.
  • This embodiment allows the use of different sized syringes, which always automatically the dosing volume is displayed, which is delivered at the set dosing increment with each syringe used.
  • the pipette has at least one further feature of the pipette described below and its embodiments.
  • the control device determines the possible maximum number of metering steps that is possible after complete or partial filling of the syringe without refilling the syringe on the basis of the detected metering step width and data present in the control device about the syringe used. In determining the maximum number of dosing steps possible without refilling the syringe, the control device takes into account the position of the means for displacing the web detected by the means for detecting the respective position of the means for displacing the receiving body.
  • the controller may use in one embodiment instead of the detected position, a fixed value for the displacement of the drive element, for example, is stored.
  • the control device displays the metering volume determined from the metering step width and the determined maximum number of metering steps. When dosing is done, this may be indicated to the controller by the means for detecting the position which detect the position of the means for displacing the receiving body on the web.
  • means for detecting the execution of a dosing step by actuating the control knob are present for this purpose, for example, sensors or buttons that emit signal with each actuation of the control knob.
  • the control device is designed such that the display of the number of dosing steps carried out and no display of the number of dosing steps still possible without refilling the syringe.
  • the Control device designed such that the display of the still possible number of metering steps without refilling the syringe instead of the number of performed metering steps.
  • the control device is such that the number of metering steps performed and at the same time the number of metering steps still possible without refilling the syringe is displayed.
  • the displacement of the displacement element can be detected in a particularly simple and precise manner.
  • Suitable incremental encoders and sensors are available as prefabricated systems. The systems are capable of detecting and indicating the movements of the incremental encoder in different directions.
  • the pitch of the incremental encoder can be selected according to the accuracy requirements of liquid pick up and liquid dispensing. According to a preferred embodiment, the pitch is selected from the range of 0.1 to 0.5 mm. For example, it is 0.1 mm, 0.25 mm or 0.5 mm.
  • the means for detecting the respective position of the means for displacing comprise a capacitive sensor.
  • the capacitive sensor has at least one arranged on a displacement element of the means for displacing the first capacitor plate and at least one fixedly arranged in the housing, the second capacitor plate, wherein the first and the second capacitor plate are connected to the control device and the control means, the capacity between determined the first and second capacitor plates.
  • the capacity between the first and second capacitor plates is a measure of the coverage of the first and second plates and thus for the respective position of the means for displacing.
  • the capacitive sensor is designed, for example, as in the CA 2,126,934C (Page 10, lines 3 to 26), the content of which is hereby incorporated into the present application.
  • the displacement element is a rack whose lower end is connected to the receiving body, which is engageable with a pivotally mounted on the control knob latch when the control knob is actuated to the rack upon actuation of the control knob to a dosing relocate.
  • the incremental encoder is integrally formed with the rack or a fixed to the rack connected component.
  • the incremental encoder can also be formed by the teeth of the rack.
  • the incremental encoder is formed on a different side of the rack than the toothing.
  • the incremental encoder is glued to the displacement element, injected into this or printed on this.
  • the means for detecting a displacement comprise a rotary encoder, which is coupled via a gear transmission or other gear with the displacement element and whose sensor is connected to the control device.
  • the pipette has no separate sensors for detecting the lowest and highest end position of the rack.
  • the respective position of the rack is detected by means of the incremental encoder.
  • the upper and lower end position may optionally be marked by means of special markings on the incremental encoder, for example by a particularly wide graduation mark on an optical scale or by successive poles of the same name in the case of a magnetic strip.
  • a separate marking of the toothed rack may possibly be dispensed with entirely by using the teach function to connect a first tooth or a first group of teeth to the lower end position and to a second tooth or a second group of teeth Rack is assigned to the upper end position.
  • control device is designed such that, after carrying out the maximum number of metering steps and refilling the same syringe, the total number of metering steps performed with the syringe with the set metering increment is determined and / or the number of metering steps still possible without refilling the syringe is determined and indicated by the display device.
  • control device is designed to redetermine the number of dosing steps performed each time a syringe is inserted.
  • control device is such that after releasably connecting the syringe to the pipette and before terminating the filling of the syringe with liquid, the maximum number of metering steps possible with the set metering step width and completely filled syringe without refilling the syringe Indicator displays.
  • control device is arranged to detect the completion of the filling of the syringe by stopping the displacement of the drive element and / or reversing the direction of the displacement of the drive element.
  • control device is such that when sucking in liquid by operating the elevator lever to the execution of the reverse stroke by pressing the control knob by means of the display device and / or by means of another output device of the pipette on the current process of receiving liquid in the syringe is issued indicative indication, signal or other output upon the execution of the reverse stroke, and the output is not output prior to aspiration of liquid and after performing the reverse stroke.
  • control device is such that by means of the display device, a display is only displayed when a syringe is detachably connected to the pipette and the display goes out when the syringe is released from the pipette.
  • control device is such that when the dosing step size is set again after the reversal stroke has been performed and before the maximum number of dosing steps possible without refilling the syringe, the display device sets the maximum possible number with the newly set dosing step width without refilling the syringe Dosing steps and the dosing volume set via the newly set dosing increment.
  • control device is such that, in the case of an incompletely executed dosing step, the pipette displays, or a signal, a signal via the display device or via another output device or another, indicative of the incomplete dosing step issuing output and / or outputs the number of completely dispensed dosing and / or outputs the number of possible without refilling the syringe still possible dosing.
  • control device is such that after an incompletely executed dosing step, the number of dosing steps performed is no longer counted until the syringe is completely emptied or until a complete dosing step is performed and until the pipette is completely emptied or completed by means of the display device or by means of another output device outputs a display, a signal or another output indicative of the incomplete dispensing of liquid.
  • the pipette has a sensor for detecting a coding indicating the syringe volume on a syringe detachably connected to the pipette.
  • the control device always determines the set metering volume based on the set metering step size and the determined syringe volume and displays it via the display device.
  • the sensor is a ring sensor.
  • the sensor for detecting a coding is according to a preferred embodiment at the same time the means for detecting a syringe detachably connected to the pipette.
  • control device is designed such that, based on the detected position of the drive element on the web, the amount of liquid taken up during filling of the syringe is determined and displayed by means of the display device.
  • control device is designed such that the dosing volume is determined on the basis of the set dosing increment and the recorded coding of a syringe held by the pipette and displayed by means of the display device.
  • the pipette is adapted to carry out the method according to one of the embodiments described in claims 1 to 11 and described on pages 5 to 13 of the description.
  • the control device is designed, in accordance with the method according to one of the further embodiments, to process the signals supplied by the means for detecting and to control the display device and an optional further output device.
  • the pipette is a pipette driven by the user's muscular strength.
  • the pipette has mechanical drive means for driving the receiving body, which can be driven by the muscular force of the user.
  • the means for holding the fastening portion in the receptacle and the further fastening portion in the further receptacle are preferably formed as described in the above-mentioned prior art documents.
  • the means for displacing the receiving body within the housing are preferably formed as described in the prior art documents mentioned above.
  • the sensor for detecting a coding of the syringe and the coding of the syringe are preferably designed as described in the prior art documents mentioned above. In this regard, reference is made to the documents mentioned above DE 29 26 691 C2 . US 4,406,170 A . DE 4 437 716 C2 . EP 0 679 439 B1 . US 5,591,408 A . EP 0 656 229 B1 .
  • the syringe 3 is arranged with the flange 17 in a receptacle 20 at the lower end of the housing 2, which at the lower end of the housing 2 has an axially directed opening 21.1 for insertion and removal of the syringe 3.
  • the syringe 3 presses with its upper side against a pressure-sensitive ring sensor 22, which scans projections on the upper edge of the flange 17.
  • the flange 17 is held in this position by means of gripping levers 23 in the housing 2.
  • the receiving body 25 is fixedly connected to a rack 27 which extends below the slot 4 in the longitudinal direction of the housing 2.
  • a metering lever 34 is pivotally mounted in a swivel bearing 32 in a bulge 33 of the slot 4 opposite side wall of the housing 2.
  • the metering lever 34 has two spaced apart legs 35, 36 which protrude on the opposite side wall of the housing 2 from the two slots 6, 7.
  • the control knob 8 is fixed to the protruding from the housing 2 ends of the legs 35, 36.
  • the pipette in the housing has a transfer member 50 for controlling a reverse stroke when the syringe 3 is completely filled.
  • Details of the transmission link 50 and its function are in the EP 2 656 916 A1 in Sections 71 to 74 and 81 to 83 and the figures referred to therein, the contents of which are hereby incorporated by reference in the present application.
  • Fig. 6 shows another embodiment, in which the translational movement of the rack 27 is transmitted via a further toothing 51 of the rack 27 to a pinion 52 which is connected to a rotary encoder 53.
  • the rotary encoder 53 is connected by a cable to the controller 42 to notify the respective position of the rack 27.
  • an optical sensor 55 scans a toothing 39, 51 of the toothed rack 27. This may be the toothing 39, which cooperates with the pawl 37, or a further toothing 51.
  • the optical sensor 55 is connected via a cable to the control device 42 in order to inform the latter of the position of the toothed rack 27.
  • the user changes the setting of the metering volume by means of the dial 10 and the changed metering volume is displayed by the display 9 (block 61).
  • control device 42 After performing the reversing stroke to be performed by single or multiple actuation of the control knob 8, the control device 42 controls the display 9 so that it stops flashing (block 63).
  • the execution of the reverse stroke is detected by the control electronics 42 due to the detected by the magnetic sensor 49 displacement of the rack 27.
  • control electronics 42 displays the sum of all dosing steps carried out (block 65).
  • the residual liquid is dispensed by operating the elevator lever 5. Then controls the controller 42 except the display of the set dosing volume and the dosing steps reached a flashing of the display 9 (block 66).
  • the blocks 60 to 64 correspond Fig. 9 .
  • the controller 42 controls that the indicator goes off (block 67).

Claims (15)

  1. Procédé pour le dosage de liquide au moyen d'une pipette avec un incrément de dosage réglable, d'un dispositif d'affichage, d'un levier de levage pour la réception de liquide dans une seringue et d'un bouton de commande pour la distribution progressive de liquide à partir de la seringue et de la seringue actionnable au moyen de la pipette, dans lequel
    1.1 la seringue est reliée de façon détachable à la pipette,
    1.2 l'incrément de dosage est réglé sélectivement ou l'incrément de dosage réglé précédemment est maintenu,
    1.3 le volume de dosage réglé par le biais de l'incrément de dosage est affiché au moyen du dispositif d'affichage,
    1.4 du liquide est aspiré dans la seringue par actionnement du levier de levage,
    1.5 la position respective d'un élément d'entraînement d'une mécanique d'entraînement de la pipette pour le remplissage et le vidage de la seringue est détectée lors d'un déplacement de l'élément d'entraînement le long d'une voie,
    1.6 le nombre maximal d'incréments de dosage possible avec l'incrément de dosage réglé sans nouveau remplissage de la seringue, après exécution de la course de retour, à partir de la position atteinte à la fin du remplissage par l'élément d'entraînement sur la voie, est détecté et affiché au moyen du dispositif d'affichage,
    1.7 après le remplissage complet ou partiel de la seringue avec du liquide, la course de retour est exécutée par au moins un actionnement unique du bouton de commande,
    1.8 des incréments de dosage sont exécutés par actionnement du bouton de commande, les incréments de dosage exécutés sont comptés et le nombre d'incréments de dosage exécutés et/ou le nombre d'incréments de dosage encore possibles sans nouveau remplissage de la seringue est/sont détecté(s) et affiché(s) au moyen du dispositif d'affichage, et
    1.9 après exécution du nombre maximal d'incréments de dosage possibles sans nouveau remplissage de la seringue, la seringue est détachée de la pipette ou les étapes 1.2 à 1.8 sont répétées au moyen de la même seringue, sachant qu'à l'étape 1.8, le nombre total d'incréments de dosage exécutés à l'aide de la seringue avec l'incrément de dosage réglé est compté et/ou le nombre d'incréments de dosage encore possibles sans nouveau remplissage de la seringue est détecté et affiché au moyen du dispositif d'affichage.
  2. Procédé selon la revendication 1, dans lequel la pipette émet une indication et un signal et/ou une autre sortie indiquant l'exécution du nombre nécessaire d'actionnements du bouton de commande, jusqu'à ce que et/ou lorsque la course de retour à exécuter soit/est exécutée.
  3. Procédé selon la revendication 1 ou 2, dans lequel, après l'assemblage détachable de la seringue avec la pipette, avant la fin du remplissage de la seringue avec du liquide, le nombre maximal d'incréments de dosage possibles après l'exécution de la course de retour sans nouveau remplissage de la seringue avec l'incrément de dosage réglé et une seringue complètement remplie, est affiché au moyen du dispositif d'affichage.
  4. Procédé selon l'une des revendications 1 à 3, dans lequel la fin du remplissage de la seringue est constatée au fait que le déplacement de l'élément d'entraînement est stoppé et/ou que la direction du déplacement de l'élément d'entraînement est inversée.
  5. Procédé selon l'une des revendications 1 à 4, dans lequel, lors de l'aspiration de liquide par actionnement du levier de levage, jusqu'à l'exécution de la course de retour par actionnement du bouton de commande, une indication, un signal ou une autre sortie, indiquant le déroulement actuel de la réception de liquide dans la seringue et de l'exécution de la course de retour, est émis(e) au moyen du dispositif d'affichage et/ou au moyen d'un autre dispositif de distribution de la pipette, et la sortie n'est pas émise avant l'aspiration de liquide ni après l'exécution de la course de retour.
  6. Procédé selon l'une des revendications 1 à 5, dans lequel une indication est affichée au moyen du dispositif d'affichage seulement lorsqu'une seringue est reliée de façon détachable à la pipette, et dans lequel l'indication disparaît lorsque la seringue est détachée de la pipette.
  7. Procédé selon l'une des revendications 1 à 6, dans lequel, lors d'un nouveau réglage de l'incrément de dosage, après exécution de la course de retour et avant l'exécution du nombre maximal d'incréments de dosage possibles sans nouveau remplissage de la seringue, le dispositif d'affichage indique le nombre maximal d'incréments de dosage possibles avec le nouvel incrément de dosage réglé sans nouveau remplissage de la seringue ainsi que le volume de dosage réglé par le biais du nouvel incrément de dosage réglé.
  8. Procédé selon l'une des revendications 1 à 7, dans lequel, lors d'un incrément de dosage exécuté de façon incomplète, la pipette émet une indication, un signal ou une autre sortie indiquant l'incrément de dosage exécuté de façon incomplète, par le biais du dispositif d'affichage ou d'un autre dispositif de sortie et/ou émet le nombre d'incréments de dosage exécutés de façon complète et/ou émet le nombre d'incréments de dosage encore possibles sans nouveau remplissage de la seringue.
  9. Procédé selon la revendication 8, dans lequel, après un incrément de dosage exécuté de façon incomplète, le nombre d'incréments de dosage exécutés n'est plus compté, jusqu'à ce que la seringue soit entièrement vide ou jusqu'à l'exécution d'un incrément de dosage complet et jusqu'au vidage complet de la seringue ou jusqu'à l'exécution complète d'un incrément de dosage, la pipette émet une indication, un signal ou une autre sortie indiquant la distribution incomplète de liquide, au moyen du dispositif d'affichage ou au moyen d'un autre dispositif de sortie.
  10. Procédé selon l'une des revendications 1 à 9, dans lequel la quantité de liquide reçue lors du remplissage de la seringue est détectée à l'aide de la position détectée de l'élément d'entraînement sur la voie et affichée au moyen du dispositif d'affichage.
  11. Procédé selon l'une des revendications 1 à 9, dans lequel la pipette détecte un codage indiquant un volume de seringue de la seringue reliée à celle-ci, et le volume de dosage est déterminé sur la base de l'incrément de dosage réglé et du codage détecté, et affiché au moyen du dispositif d'affichage.
  12. Pipette (1) pour la manipulation d'une seringue (3), comprenant
    - un boîtier en forme de barre (2),
    - un logement (20) avec une ouverture (21.1) à l'extrémité inférieure du boîtier (2), pour l'insertion d'une seringue (3) avec une section de fixation (16) sur le bord supérieur d'un cylindre (11),
    - un corps de logement (25) avec un autre logement (24) et une autre ouverture (21.2) à l'extrémité inférieure dans le boîtier (2), pour l'insertion d'une autre section de fixation (19) d'un piston (12) de la seringue (3),
    - des moyens pour le maintien détachable de la section de fixation (16) dans le logement (20) et de l'autre section de fixation (19) dans l'autre logement (24),
    - au moins un moyen de déplacement du corps de logement (25) à l'intérieur du boîtier (2) dans le sens longitudinal du boîtier (2),
    - lesquels présentent un levier de levage (5) actionnable à l'extérieur du boîtier (2) pour la réception de liquide dans la seringue (3),
    - un bouton de commande (8) actionnable à l'extérieur du boîtier (2) pour la distribution progressive de liquide à partir de la seringue (3), et
    - un élément de réglage réglable à l'extérieur du boîtier (2) pour le réglage d'un incrément de dosage,
    - des moyens de détection de l'incrément de dosage réglé au moyen de l'élément de réglage,
    - des moyens de détection d'une seringue (3) reliée de façon détachable à la pipette (1),
    - des moyens de détection de la position respective du moyen de déplacement du corps de logement (25) lors du déplacement du moyen de déplacement le long d'une voie par rapport au boîtier (2),
    - un dispositif de commande (42) relié électroniquement aux moyens de détection, et
    - un dispositif d'affichage (9) relié électroniquement au dispositif de commande électronique (42),
    - dans lequel, lorsque la seringue (3) est insérée dans la pipette (1), le dispositif de commande (42) est conçu pour détecter et afficher, au moyen du dispositif d'affichage (9), le volume de dosage à l'aide de l'incrément de dosage réglé ainsi que le nombre maximal d'incréments de dosage possibles sans nouveau remplissage de la seringue (3) après l'exécution de la course de retour, avec l'incrément de dosage réglé et une seringue (3) entièrement ou partiellement remplie, à l'aide de la position du moyen de déplacement sur la voie, pour détecter l'exécution d'une course de retour à exécuter par au moins un actionnement unique du bouton de commande (8), et pour émettre une sortie indiquant ceci au moyen du dispositif d'affichage (9) ou d'un autre dispositif de sortie, jusqu'à l'exécution de la course de retour et/ou après exécution réussie de la course de retour, pour détecter le nombre d'incréments de dosage exécutés, et pour afficher le nombre d'incréments de dosage exécutés et/ou le nombre d'incréments de dosage encore possibles sans nouveau remplissage de la seringue (3), au moyen du dispositif d'affichage (9).
  13. Pipette (1) selon la revendication 12, dans laquelle les moyens de détection de la position respective du moyen de déplacement présentent un capteur incrémental disposé sur un élément de déplacement des moyens de déplacement, et au moins un détecteur (44) disposé fixement sur le boîtier (2) et relié au dispositif de commande (42) pour la détection du déplacement du capteur incrémental.
  14. Pipette (1) selon la revendication 13, dans laquelle l'élément de déplacement consiste en une crémaillère (27), dont l'extrémité inférieure est reliée au corps de logement (25), laquelle peut être engagée avec un cliquet (37) monté de façon pivotante sur le bouton de commande (8), lorsque le bouton de commande (8) est actionné, pour déplacer la crémaillère (27) selon un incrément de dosage lors de l'actionnement.
  15. Pipette (1) selon la revendication 13 ou 14, dans laquelle le capteur incrémental est formé d'un seul tenant avec la crémaillère (27) ou consiste en une pièce reliée fixement à la crémaillère (27).
EP16204865.6A 2016-12-16 2016-12-16 Procédé de dosage de liquide au moyen d'une pipette et d'une seringue et pipette destinée à actionner une seringue pour le dosage de liquides Active EP3335795B1 (fr)

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Application Number Priority Date Filing Date Title
EP16204865.6A EP3335795B1 (fr) 2016-12-16 2016-12-16 Procédé de dosage de liquide au moyen d'une pipette et d'une seringue et pipette destinée à actionner une seringue pour le dosage de liquides
PL16204865T PL3335795T3 (pl) 2016-12-16 2016-12-16 Sposób dozowania cieczy za pomocą pipety i strzykawki oraz pipeta do uruchamiania strzykawki do dozowania cieczy
CN201711349275.6A CN108201903B (zh) 2016-12-16 2017-12-15 用移液器和注射器配给液体的方法和操作配给液体的注射器的移液器
JP2017241011A JP6639461B2 (ja) 2016-12-16 2017-12-15 ピペットおよびシリンジを用いて液体を投与する方法、および液体を投与するシリンジを作動するためのピペット
US15/844,921 US11154854B2 (en) 2016-12-16 2017-12-18 Method for dosing liquid by means of a pipette and a syringe, and pipette for actuating a syringe for dosing liquid
US16/934,243 US11697113B2 (en) 2016-12-16 2020-07-21 Method for dosing liquid by means of a pipette and a syringe, and pipette for actuating a syringe for dosing liquid

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CN108201903A (zh) 2018-06-26
US11697113B2 (en) 2023-07-11
JP6639461B2 (ja) 2020-02-05
JP2018140385A (ja) 2018-09-13
EP3335795A1 (fr) 2018-06-20
US20210001326A1 (en) 2021-01-07
US11154854B2 (en) 2021-10-26
PL3335795T3 (pl) 2019-09-30
CN108201903B (zh) 2020-09-18
US20180169647A1 (en) 2018-06-21

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