EP3164214B1 - Pipette servant à faire fonctionner une seringue - Google Patents

Pipette servant à faire fonctionner une seringue Download PDF

Info

Publication number
EP3164214B1
EP3164214B1 EP15732592.9A EP15732592A EP3164214B1 EP 3164214 B1 EP3164214 B1 EP 3164214B1 EP 15732592 A EP15732592 A EP 15732592A EP 3164214 B1 EP3164214 B1 EP 3164214B1
Authority
EP
European Patent Office
Prior art keywords
cover part
cover
rack
section
pipette
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP15732592.9A
Other languages
German (de)
English (en)
Other versions
EP3164214A1 (fr
Inventor
Burkhardt Reichmuth
Jörg SATTLER
Marc Wilth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to PL15732592T priority Critical patent/PL3164214T3/pl
Publication of EP3164214A1 publication Critical patent/EP3164214A1/fr
Application granted granted Critical
Publication of EP3164214B1 publication Critical patent/EP3164214B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0234—Repeating pipettes, i.e. for dispensing multiple doses from a single charge
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices

Definitions

  • the invention relates to a pipette for actuating a syringe.
  • the pipettes used here for actuating a syringe serve to dispense liquid received in the syringe in several steps. They are also referred to as dispensers or repeating pipettes. These pipettes have at the lower end of a rod-shaped housing a receptacle for a flange of a cylinder of the syringe and in the housing a displaceable receiving body with a piston receptacle for the upper end portion of a piston rod of a piston of the syringe.
  • the syringe can be used with the flange and the end portion of the piston rod through axially directed openings of the receptacles.
  • the flange and the end portion are held in the receptacles by means for detachable holding, which are designed, for example, as spring-loaded gripping levers.
  • the pipette comprises means for displacing the receiving body, which allow a partial withdrawal of the piston from the cylinder for the suction of liquid into the syringe and a stepwise depression of the piston into the cylinder for the stepwise discharge of liquid.
  • the DE 29 26 691 C2 and the US 4,406,170 A describe means for displacing the receiving body in the housing. These include a connected to the receiving body and by a straight line slot protruding from the housing elevator lever for drawing liquid into the syringe by displacing the receiving body of the recording away. In addition, they include a rack-pawl means for gradually advancing the piston by a reciprocating metering lever. At the metering a pivoting pawl is mounted. The rack is connected to the receiving body and arranged in the pivoting range of the pawl. An adjustable sliding cover covers the row of teeth on the rack more or less to limit the engagement of the pawl in the rack when pivoting the metering lever.
  • the sliding cover is displaceable by means of a rotary knob, which is arranged on the pipette housing.
  • the knob is designed with an eccentric guide curve, in which engages a guide pin of the sliding cover.
  • the rack is designed with a lofty approach and the cover has an obliquely downwardly directed offset, which engages with the approach at far advanced piston, whereby the cover of the rack is displaced away, so that it prevents the pawl from to intervene in the uncovered teeth of the rack. This Resthubsperre prevents that a residual amount is discharged from the syringe, which is smaller than the dispensed at each dosing dose (dispensing volume).
  • the EP 0 657 216 B1 and US Pat. No. 5,620,661 A describe such a pipette with a sensor for sensing elevations and depressions on the syringe flange of syringes and correspondingly configured syringes.
  • the sensor is used to determine the size of the syringe used.
  • electronics determines the amount of fluid dispensed at each dispensing step. This is displayed on a display.
  • the cover has an elongated sleeve made of plastic, in which a threaded rod is displaceably arranged.
  • a metal sleeve is glued in the lower end of the plastic sleeve.
  • the metal sleeve protrudes from the lower end of the plastic sleeve and has an incidence edge below, below which the thread is exposed for engaging the pawl.
  • the pawl first slides over the metal sleeve, which prevents the pawl from engaging in the thread of the threaded rod. As soon as the pawl passes the incidence edge during the dosing step, it falls into the thread.
  • the pawl meets a flank or the apex of the thread, which is arranged below the thread root, too little part of the pivoting movement of the metering lever is used for the displacement of the threaded rod and thus of the piston. Also in this case too little liquid is dispensed. It may even happen that the pawl slips off the flank or the apex of the thread and falls further down into the thread root or several times slipped over the thread, which leads to an increased dosing error or no liquid is discharged.
  • the dosing error can be overcome by adjusting the threaded rod.
  • the threaded rod is screwed into a setting thread in the receiving body for the upper end of the piston rod and provided with a transverse bore.
  • a metal strip is fixed on the plastic sleeve, which has a projecting angled portion and a parallel to the rack extending spring tongue in a reboundable section.
  • the metal strip is held on plastic domes, the ends of which are melted and formed by means of ultrasound.
  • the rack has at the end of a cam whose position is adjustable in the axial direction of the rack. At the end of the feed movement of the Piston meets the cam on the bend, whereby the deflectable portion of the metal strip deflected and the pawl is prevented from further engagement with the rack. As a result, the Resthubsperre is realized.
  • the DE 10 2012 011 938 A1 describes a pipette in which the cover has a first cover part made of plastic, on which the guide pin is formed. On the first cover part, a second cover part is arranged in the form of a metal strip on which the incidence edge is formed and which has the angled portion and the spring tongue of Resthubsperre.
  • the relative position of the first and the second cover part to each other in the longitudinal direction of the rack is adjustable by means of an eccentric of the cover. As a result, the distance from the incidence edge and guide pin is exactly adjusted to ensure despite manufacturing tolerances or wear or repair that the pawl always exactly falls into the rack.
  • the adjustment of the volume of liquid to be dispensed at each step is also carried out by means of a rotary knob.
  • the engagement of the pawl in the rack by means of the knob is adjustable.
  • the knob on two knob parts whose angular position is adjustable to each other via a transmission.
  • a rotary knob part has markings for adjusting the dosing volume.
  • the other knob part has the guide cam engaged by the pin projecting from the cover. Turn the knob to move the cover along the rack.
  • the EP2676730 describes a pipette for actuating a syringe with a rod-shaped housing, a receiving body for inserting the syringe, a rack connected to the receiving body and a rack disposed on the rack arranged cover consisting of a first and a second cover part.
  • the invention is based on the finding that for the optimal engagement of the pawl and the correct triggering of Resthubsperre compliance with defined distances of coupling element and incident edge and incident edge and angling and accurate positioning of the cam with respect to the toothing on the rack are relevant. Furthermore, the invention is based on the finding that the production methods used hitherto (sheet metal processing and injection molding) and joining methods (gluing, forming of retaining domes and screwing) do not allow sufficient manufacturing tolerances for this purpose.
  • the second cover part is first inserted into an injection mold, wherein the bend is supported on a contact surface of the injection molding and then encapsulated with the mold partially closed with the plastic of the first cover part.
  • the rack is preferably molded in one piece with the plastic cam.
  • the rack is provided with an adjustable cam to compensate for remaining inaccuracies.
  • a head portion of the second cover part adjacent to an upper slot portion of the longitudinal slot on the rack side facing the edge of the plastic of the first cover part molded and the head portion is connected at the lower end with the bend, which in a lower slot portion of Longitudinal slot is arranged next to the second cover member is not molded with the plastic of the first cover member, so that the cam in the longitudinal slot can slide along the head portion before he meets the bend.
  • This embodiment avoids a lateral deflection of the cam when entering the slot and thereby avoids dosing errors caused thereby.
  • the second cover part has a first strip-shaped connected to the lower end of the head portion Section which is connected at the lower end with the bend and arranged in the lower slot portion.
  • the first strip-shaped section forms a spiral spring, which facilitates the deflection of the bend by the cam.
  • the second cover part has a third strip-shaped section connected to the lower end of the bend, which is arranged in the lower slot section and on which the cam can slide after passing through the bend.
  • a connecting portion is connected to a longitudinal edge of the first or second strip-shaped portion, extends through a recess in the top of the first cover member to the longitudinal edge of the first cover member and extending over a deflection parallel to the longitudinal side of the first cover member down connected to the spring tongue.
  • the second cover part is resilient.
  • the second cover part is made in one piece from sheet metal, ie from a flat, thin-walled metal sheet.
  • the wall thickness of the sheet is a maximum of 0.5 mm, preferably 0.4 mm.
  • stamping, lasering, etching, bending, wire EDM, water jet cutting preferably one or more of the following sheet metal processing methods are used: stamping, lasering, etching, bending, wire EDM, water jet cutting.
  • the adjusting element is a dial.
  • the guide curve is spiral.
  • the second cover part is covered on the side facing away from the rack side of the first cover part of transverse to the rack extending webs of the first cover part.
  • the webs bridge the longitudinal slot on the side facing away from the rack and keep the first cover part together at the top to save material.
  • a web carries the coupling element.
  • the coupling element is a protruding from the first cover part pin and the adjusting element has a guide curve on the underside, in which engages the pin, so that upon adjustment of the adjusting element, the guide cam moves the pin in the axial direction of the rack.
  • the lower end of the first cover part on the incidence edge This allows a particularly accurate positioning of the incidence edge with respect to the coupling element.
  • the first cover part has a wall thickness gradually decreasing towards the lower end.
  • the incidence edge has a particularly short distance from the rack, which promotes that the pawl always coincides with their teeth exactly in the toothing of the rack. Due to the greater wall thickness of the first cover part above the incidence edge, a sufficient breaking strength can be achieved.
  • the first cover part at the engagement edge has a wall thickness of at most 0.5 mm, preferably from 0.2 to 0.4 mm.
  • the first cover part with the planar contact surface on the side facing away from the rack side to the lower end to a chamfer As a result, a particularly small distance of the incidence edge is achieved by the rack.
  • the first cover part has the shape of an elongated hollow body with two parallel, strip-shaped side parts and a base defining a channel, wherein the longitudinal slot is formed in the base and the second cover part is encapsulated in the base of the plastic of the first cover part , In the channel, the rack can be accurately guided.
  • the first cover part on the two longitudinal sides laterally projecting wings.
  • the wings can be used for precise guidance of the cover part in the housing.
  • the rack with the cam is integrally molded from plastic.
  • the first cover part and / or the rack made of PEEK or of another plastic with similar mechanical properties.
  • the pawl on a plurality of ratchet teeth, which engage in the toothing of the rack after passing the incidence edge.
  • the invention also includes embodiments in which the pawl has only a single pawl tooth.
  • the cam is arranged einjustierbar on the rack in the axial direction.
  • the cam on the side of the Abhalteelements protrudes through a slot of the rack and has an axial bore, an adjusting screw is screwed through the bore into a Einstellgewinde at the upper end of the rack and is a spring element between the upper end of the Rack and arranged the cam.
  • This configuration allows adjustment of the axial position of the cam on the rack. As a result, the position of the rack is affected, in which the cam meets the second cover part and the Resthubsperre is effective.
  • the first cover part made of plastic and / or the second cover part is made of a metal sheet.
  • the first cover part is preferably made by injection molding and / or the second cover part by etching and / or punching and / or laser and / or wire EDM and / or water jet cutting and / or bending a sheet or other manufacturing processes.
  • the means for releasably holding the first attachment portion in the first receptacle and the second attachment portion in the second receptacle are preferably formed as in EP 0 656 229 B1 and US 5,620,660 A or in the EP 1 724 120 B1 and the US 7,731,908 B2 described.
  • the first means for displacing the receiving body in the housing are preferably designed as an elevator lever, as in the DE 29 26 691 C2 and the US 4,406,170A or the references cited in the preceding paragraph.
  • the pipette has a sensor for scanning elevations and depressions on the syringe flange of the syringes, as in US Pat EP 0 657 216 B1 and the US 5,620,661A described.
  • the term "rack" both a rack in the true sense, d. H. a rod with a series of teeth arranged thereon, as well as a threaded rod, d. H. a rod with at least one thread arranged thereon, addressed.
  • the pipette according to the invention is provided with a rack in the true sense.
  • top and bottom refer to the orientation of the pipette in which the bar-shaped housing is vertically aligned and the syringe receptacle is located below ,
  • a pipette 1 has a rod-shaped housing 2 in which a syringe 3 is held at the bottom. From a side wall of the housing 2 is above a straight line slot 4 an elevator lever 5 of the housing 2 in front. From the same side wall of the housing 2 is about two further slots 6, 7, a control knob 8 of a rack-pawl control before.
  • a display device in the form of a display 9 is embedded in the same side wall of the housing 2. From openings in the adjacent side wall are segments of a dial 10 out.
  • the syringe 3 has a cylinder 11 and a piston 12 arranged displaceably therein.
  • the cylinder 11 has at the bottom a conical portion 13 with a hole 14 for the passage of liquids and above a cylindrical portion 15 in which the piston 12 is displaceable.
  • the cylinder 11 has a first mounting portion 16 with a circumferential flange 17.
  • From the piston 12 is upwardly a piston rod 18 which has a second mounting portion 19 with a plurality of circumferential beads.
  • the syringe 3 is arranged with the flange 17 in a first receptacle 20 at the lower end of the housing 2, which at the lower end of the housing 2 has an axially directed first opening 21 for insertion and removal of the syringe 3.
  • the syringe 3 presses with its upper side against a pressure-sensitive ring sensor 22, which scans projections on the upper edge of the flange 17.
  • the indicated on the flange 17 code denotes the size of the respective syringe 3.
  • the flange 17 is held in this position by means of first means for releasably holding in the form of first gripping levers 23 in the housing 2.
  • the second attachment portion 19 of the piston 12 is arranged in a second receptacle 24 in a hollow cylindrical receiving body 25. This has for insertion of the second attachment portion 19 on an axially directed second opening 26.
  • the second attachment portion 19 is by means of second means for releasably holding held in the form of second gripping levers 27 which engage between the beads of the second mounting portion 19 or pinch this.
  • the receiving body 25 is fixedly connected to a rack 28 with a toothing 29 which extends below the slot 4 in the longitudinal direction of the housing 2.
  • An elevator lever holder 30 is fixed to the receiving body 25 and to a lower part of the rack 28.
  • an elevator lever carrier 31 is present, which abuts with a sliding plate 32 at the bottom of the edges of the slot 4.
  • the elevator lever carrier 31 has an upwardly projecting post 33, which passes through the slot 4.
  • the elevator lever 5 is fixed outside the housing 2.
  • an actuating element in the form of a metering lever 36 is pivotably mounted in a swivel bearing 34 in a bulge 35 of the slot 4 opposite side wall of the housing 2.
  • the metering lever 36 has according to Fig. 3 to 6 two spaced legs 37, 38 which protrude on the opposite side wall of the housing 2 from the two slots 6, 7. There, the control knob 8 is fixed to the protruding ends of the legs 37, 38.
  • the elevator lever carrier 31 strikes a fixedly arranged in the housing 2 bearing body in which the pivot bearing 34 is formed for the metering lever 36. As a result, the upper stop for the displacement of the receiving body 25 is given.
  • a pawl 39 is pivotally mounted between the two legs 37, 38 of the metering lever 36.
  • the pawl 39 is arranged with a plurality of ratchet teeth 40 (three in the example) above the toothing 29 of the rack 28.
  • the metering lever 36 is moved from a spring device to the position of Fig. 2 pressed. Contrary to the action of the spring device of the metering lever 36 by pressing the control knob 8 is pivoted downwards.
  • the pawl 39 is pressed by means of a further spring means to the toothing 29 of the rack 28. As a result, the rack 28 is pushed down.
  • a sliding cover 41 is arranged between the pawl 39 and the rack 28 .
  • the cover 41 is displaceable by turning the laterally projecting out of the housing 2 dial 10 so that it covers the teeth 29 of the rack 28 more or less.
  • the pawl is first pressed against the cover 41 upon actuation of the control knob 8 and falls under it into the toothing 29 a. Therefore, the amount of displacement of the rack bar 28 when pivoting the metering lever 36 downward depends on the position of the cover 41 with respect to the toothing 29.
  • the cover 41 is made according to Fig. 7 from a first cover part 42 made of plastic and a second cover part 43 made of a thin sheet metal, which is partially embedded by encapsulation in the plastic of the first Abeckungsteils 42.
  • the second cover part 43 has a head portion 44, which in the example has the shape of a wide strip.
  • the head portion 44 has at the bottom on the two longitudinal sides opposing notches 45, 46, which serve the anchorage in the plastic of the first cover part 42. Below this, the head portion 44 has a taper 47.
  • the lower end of the taper 47 is connected to a first strip-shaped portion 48.
  • the first strip-shaped section 48 is connected via a counter-angled bend 49 with a second strip-shaped section 50.
  • the head portion 44, the first strip-shaped portion 48 and the second strip-shaped portion 50 have a common longitudinal axis.
  • the second strip-shaped portion 50 is connected in the upper part on one longitudinal side with a strip-shaped connecting portion 51 which protrudes perpendicularly from the second strip-shaped portion 50.
  • the connecting portion 51 is connected via a deflection 52 with a spring tongue 53.
  • the second cover part 43 is made in one piece from a thin sheet metal. This is preferably a resilient material, in particular a spring steel.
  • the first cover part 42 is generally in the form of an elongate hollow body having a U-shaped cross section. It has two parallel strip-shaped side parts 54, 55 and a base 56 bridging these. The side parts 54, 55 and the base 56 have inside planar contact surfaces for guiding the rack 28.
  • the side members 54, 55 and base 56 define a channel 57.
  • An upper base part 58 has a greater wall thickness than a lower base part 59 of the base 56.
  • Upper and lower base parts 58, 59 are connected to one another via a ramp 60, so that a smooth transition exists between the outer sides.
  • a longitudinal slot 61 extends centrally in the base 56.
  • the longitudinal slot 61 extends over the entire upper base part 57 and ends shortly before the lower end of the lower base part 59.
  • an upper slot section 62 of the longitudinal slot 61 is covered laterally by the head section 44.
  • a lower slot portion 63 of the longitudinal slot 61 extends in the lower base part 59 and is wider than the first and second strip-shaped portions 48, 50 and the angled portion 49, which are disposed within or slightly in front of the slot portion 63. Thus, a gap exists on both sides of the first and second strip-shaped section 48, 50 and the bend 49 and the lower slot section 63.
  • the head portion 44 is encapsulated within the upper base part at the edge with the plastic of the first cover part 42.
  • the width of the edge-facing plastic enclosure is less than on the side of the head portion 44, which faces the rack 28, where the edge-side enclosure defines the upper slot portion 62
  • the notches 45, 46 anchored are the notches 45, 46 anchored.
  • the second cover part 43 is fixed to the first cover part 42.
  • the portions of the second cover part 43 below the head portion 44 are not encapsulated and therefore rebuttable with respect to the first cover part 42.
  • the taper 47 or the first strip-shaped section 48 emerges from the ramp 60.
  • a coupling element 68 in the form of a pin, which is injection-molded in one piece with the first cover part 42.
  • the first cover part 42 has in the outer side a recess 69 which extends from the lower slot portion 63 to the lateral edge of the side part 54. Through this recess 69, the connecting portion 51 extends to the side, so that the spring tongue 53 extends at a small distance from the first cover part 42.
  • the first cover part 42 has an incident edge 70 at the lower end. This is formed at the lower end of the lower base part 59. There, the lower base part 59 has a wall thickness of at most 5 mm, preferably 4 mm or even lower.
  • the outer side surface of the lower base portion 59 is a detent surface 71 for the pawl 39.
  • the lower base portion has a chamfer 72 at the bottom on the outside. At the upper end of the chamfer 72, the lower base portion 59 has a wall thickness of 5 mm or more.
  • the incidence edge 70 is interrupted by a short, slit-like axial space 73 extending in the axial direction.
  • the cover 41 is produced, in which the second cover part 43 is inserted into an injection mold, so that the bend 49 rests against a certain contact surface. Thereafter, the tool is closed and the first cover member 42 injection molded.
  • the finished cover is characterized in that the bend 49, pin 68 and incidence edge 70 with sufficient tolerances occupy predetermined positions.
  • the rack 28 extends through the channel 57 of the first cover 42.
  • the pawl 39 is threaded with a guide slot 76 at the bottom of the spring tongue 53 of the second cover 43.
  • the cover 41 is slidably guided in the housing 2 in the longitudinal direction.
  • the cover 41 is displaceable in the longitudinal direction.
  • the dial 10 according to Fig. 9 a central bearing hole 77 and a spiral guide cam 78.
  • the dial 10 is rotatably supported on the bearing hole 77 on an axis on the housing 2 and the pin 68 of the cover 41 engages the guide cam 78 a.
  • the cover 41 is displaced along its guide in the housing 2.
  • the dial 10 is associated with a further sensor 79 which detects the rotational position of the dial 10.
  • Fig. 5 is at the upper end of the rack 28, a cam 80 molded firmly at a defined position.
  • the cam 80 Upon a downward displacement of the rack 28, the cam 80 is moved through the longitudinal slot 61 of the cover 41 and enters from the upper slot portion 62 in the lower slot portion 63 a. Finally, the cam 80 meets the protruding bend 49 and pushes them together with the spring tongue 53 forward (in Fig. 5 up). As a result, the guided on the spring tongue 53 pawl 39 is excavated from the toothing 29 and realized a Resthubsperre.
  • a transfer member 81 is arranged for controlling a Umledgehubes in the housing of the pipette.
  • the transmission member 81 has a strip-shaped transmission section 82, which is guided parallel to the rack 28 in the housing 2 of the pipette and displaced.
  • the strip-shaped transfer section 82 has a lower transfer member end 83, which is located in a displacement of the transfer member 82 down in the displacement region of a side of the rack 28 protruding lower stop 84.
  • control arms 85, 86 are connected, which are arranged parallel to each other in a plane perpendicular to the plane of the drawing.
  • the transmission member 81 is displaced so that the control arms 85, 86 reach with their upper ends in the displacement region of beveled stop edges on the legs 37, 38 (see. Fig. 2 ).
  • the transmission member 81 and the cooperating components are preferably formed as in the European patent application 120 02 849.3-2113 and in the US 13 / 867,800 described, the contents of which are hereby incorporated into the present application.
  • a printed circuit board 87 is arranged with an electronics 88.
  • an electrical power supply in the form of at least one battery 89 (eg button cell) or a battery 89.
  • the electronics 88 is wired to the ring sensor 22, the further sensor 79 for detecting the rotational position of the dial 10 and the display 9 , Further, the electronics 88 is connected to the battery 89.
  • the electronics 88 determined from the measurement signals supplied by the ring sensor 22, the respective syringe size and the setting of the dial 10, the respective step size. From this it calculates the set dispensing volume and brings it to display 9 for display.
  • a syringe 3 is first connected with a user-selected syringe size releasably connected to the pipette 1 by being inserted with the first mounting portion 16 in the receptacle 20 and with the second mounting portion 19 in the receptacle 24, so that the Flange 17 of the first gripping levers 23 and the attachment portion 19 is gripped by the second gripping levers 27.
  • This situation is in Fig. 2 shown.
  • the ring sensor 22 scans the coding on the flange 17 of the syringe 3.
  • the electronics 88 determines from the signals supplied by the ring sensor 22 that a syringe 3 is inserted and turns on the display 9.
  • the electronics 88 determines based on the signals supplied by the ring sensor 22 and the further sensor 79, the set Dispensiervolumen and brings this on the display 9 for display.
  • the user changes by means of the dial 10 the Setting the Dispensiervolumens and the changed Dosiervolumen is displayed by the display 9.
  • a reverse stroke must be carried out. This is done by pressing the control knob 8. Here meet the legs 37, 38 on the control arms 85, 86 and shift the transmission member 81 down. The transmission member 81 takes over the lower stop 84 with the rack 28. This reverse stroke removes the lots from the system.
  • the absorbed liquid amount can be delivered in small steps by the control knob 8 is pressed repeatedly against the action of the spring device down.
  • the further spring device pushes the pawl 39 with the ratchet teeth 40 against the retention surface 71 of the cover 41 until the ratchet teeth 40 reach the lower end of the retention surface 71.
  • This situation is in Fig. 10 and 11 shown.
  • the pawl 39 falls with the ratchet teeth 40 in the toothing 29 of the rack 28 and takes the rack 28 during further pivoting of the metering lever 36 down with a piece.
  • This situation is in Fig. 12 and 13 shown. Due to the precise positioning of pin 68 and incidence edge 70, the ratchet teeth 40 fall in exactly the toothing 29 a.
  • the displacement of the rack 28 with each pivoting of the metering lever 36 until reaching a lower stop depends on a set by means of the dial 10 position of the cover 41 from. After relieving the control knob 8 he is pushed by the spring device up and another dosing can be performed.
  • Residual liquid in the syringe 3 can be discharged by displacing the elevator lever 5 down. Thereafter, the syringe 3 can be separated from the pipette 1.
  • the user operates the first gripping levers 23, which act on projections on the inside of the second handle lever 27, as in the EP 0 656 229 B1 and the US 5,620,660 A described.
  • a pipette according to the invention instead of directly manually operable gripping levers 23, 27, first and second means for releasably holding the syringe, which are formed as in the EP 2 033 712 A1 and US 2009/139351 A1 described.
  • the syringe after emptying by a further actuation of the metering lever detached from the pipette.
  • the pipette on a transmission which is controlled by the metering lever and acts on the first and second means for holding the syringe to release the syringe from the pipette.
  • the first and second releasably holding means are designed as gripping levers which are integrated into the housing and can not be actuated directly from the outside.
  • each pawl tooth 40 engages exactly in a valley between two teeth of the toothing 29.
  • Fig. 14 shows a pipette with an alternatively formed rack 28th
  • the cam 80 projects from a further longitudinal slot 90 of the rack 28 to the outside. Under the further longitudinal slot 90, the cam 80 has an axial bore 91.
  • An adjusting screw 92 is screwed through the axial bore 91 into a Einstellgewinde 93 at the upper end of the rack 28.
  • a coil spring 94 is guided on the adjusting screw 92 and is supported at one end on the screw head of the adjusting screw 92 and the other end at the upper end of the rack 28 to hold the cam 80 in contact with the screw head.
  • the position of the cam 80 in the longitudinal direction of the rack 28 is variable. This allows adjustment of the Resthubsperre, if this - for whatever reason - should be required.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (17)

  1. Pipette destinée à faire fonctionner une seringue, comprenant :
    - un boîtier en forme de barre (2),
    - une première admission (20) avec une première ouverture (21) à l'extrémité inférieure du boîtier (2), pour l'insertion d'une première section de fixation (16) sur le bord supérieur d'un cylindre (11) de la seringue (3),
    - un corps d'admission avec une deuxième admission (24) et une deuxième ouverture (26) à l'extrémité inférieure dans le boîtier (2), pour l'insertion d'une deuxième section de fixation (19) sur un piston (12) de la seringue (3),
    - des premiers moyens de maintien amovible (23) de la première section de fixation (16) dans la première admission (20),
    - des deuxièmes moyens de maintien amovible (27) de la deuxième section de fixation (19) dans une deuxième admission (24),
    - des premiers moyens de déplacement (5) du corps d'admission (25) dans le boîtier (2) à distance de la première admission (20),
    - des deuxièmes moyens de déplacement du corps d'admission (25) vers la première admission (20) par pas avec une largeur de pas correspondant aux volumes de liquide à faire sortir à chaque pas par la seringue (3),
    - un élément d'actionnement (36) actionnable à l'extérieur du boîtier (2) pour l'exécution de différentes étapes,
    - une crémaillère (28) avec une denture (29) disposée dans le boîtier (2) et reliée au corps d'admission (25),
    - un loquet (39) monté de façon pivotante sur l'élément d'actionnement (36) dans le boîtier (2), lequel vient en prise dans la denture (29) de la crémaillère (28) lors d'un déplacement de l'élément d'actionnement (36) vers le bas, tout en emmenant celle-ci, et lequel se désengage de la denture (29) lors d'un déplacement de l'élément d'actionnement (36) vers le haut,
    - un élément de réglage (10) réglable à l'extérieur du boîtier (2), pour le réglage de la largeur de pas des pas,
    - un recouvrement (41) disposé de façon déplaçable sur le côté du loquet (39) sur la crémaillère (28), avec une surface de retenue (71) tournée vers le loquet (39) pour empêcher le loquet (39) de venir en prise dans la denture (29), et avec une arête biseautée (70) à l'extrémité inférieure, en dessous de laquelle la denture (29) est libre pour venir en prise avec le loquet (39),
    - un élément d'accouplement (68) disposé sur le recouvrement (41), lequel est accouplé à l'élément de réglage (10), pour transmettre un réglage de l'élément de réglage (10) au recouvrement (41),
    - dans laquelle le recouvrement présente une première partie de recouvrement (42) en plastique, laquelle comporte l'élément d'accouplement (68) et une fente longitudinale (61), ainsi qu'une deuxième partie de recouvrement (43) en tôle maintenue sur la première partie de recouvrement (42), laquelle comporte un coude (49) disposé dans la fente longitudinale (61) et une languette élastique (53) reliée à celui-ci,
    - la crémaillère présente un ergot saillant (80) sur le côté tourné vers le recouvrement, lequel touche le coude (49) à la fin du déplacement du corps d'admission (25) vers la première admission (20) dans la fente longitudinale (61), moyennant quoi la languette élastique (53) est déviée et le loquet (39) est empêché par la languette élastique (53) de revenir en prise dans la denture (29) de la crémaillère (28), caractérisée en ce que
    - l'arête biseautée (70) est formée sur la première partie de recouvrement (42),
    - la deuxième partie de recouvrement (43), à l'exception d'une section comportant au moins le coude (49) et la languette élastique (53), est enrobée par le plastique de la première partie de recouvrement (42) dans une position définie du coude (49) par rapport à l'élément d'accouplement (68), et la première partie de recouvrement (42) et la deuxième partie de recouvrement (43) étant ainsi reliées fixement entre elles.
  2. Pipette selon la revendication 1, dans laquelle une section de tête (44) de la deuxième partie de recouvrement (43) est enrobée côté bord par le plastique de la première partie de recouvrement (42) à proximité immédiate d'une section de fente supérieure (62) de la fente longitudinale (61) sur le côté tourné vers la crémaillère (28), et la section de tête (44) est reliée au coude (49) à l'extrémité inférieure, lequel est disposé dans une section de fente inférieure (63) de la fente longitudinale (61), à côté de laquelle la deuxième partie de recouvrement (43) n'est pas enrobé par le plastique de la première partie de recouvrement (42), de telle façon que l'ergot (80) peut glisser longitudinalement dans la fente longitudinale (61) sur la section de tête (44) avant de rencontrer le coude (49).
  3. Pipette selon la revendication 2, dans laquelle la deuxième partie de recouvrement (43) présente une première section en forme de bande (48) reliée à l'extrémité inférieure de la section de tête (44), laquelle est reliée au coude (49) à l'extrémité inférieure et disposée dans la section de fente inférieure (63).
  4. Pipette selon la revendication 2 ou 3, dans laquelle la deuxième partie de recouvrement (43) présente une deuxième section en forme de bande (50) reliée à l'extrémité inférieure du coude (49), laquelle est disposée dans la section de fente inférieure (63) et sur laquelle l'ergot (80) peut glisser longitudinalement après être passé par le coude (49).
  5. Pipette selon l'une des revendications 1 à 4, dans laquelle une section de liaison (51) est reliée à un bord côté longitudinal de la première ou de la deuxième section en forme de bande (48, 50), tout en s'étendant à travers un renfoncement (69) dans le côté supérieur de la première partie de recouvrement (42) jusqu'au bord côté longitudinal de la première partie de recouvrement (42), et tout en étant reliée à la languette élastique (53) s'étendant vers le bas parallèlement au côté longitudinal de la première partie de recouvrement (42) par le biais d'une déviation (52).
  6. Pipette selon l'une des revendications 1 à 5, dans laquelle la deuxième partie de recouvrement (43) est constituée de tôle et réalisée d'un seul tenant.
  7. Pipette selon l'une des revendications 1 à 6, dans laquelle la deuxième partie de recouvrement (43), sur le côté de la première partie de recouvrement (42) opposé à la crémaillère (28), est recouverte par des entretoises (64-67) de la première partie de recouvrement (42), lesquelles s'étendent perpendiculairement à la crémaillère (28).
  8. Pipette selon la revendication 7, dans laquelle une entretoise (65) porte l'élément d'accouplement (68).
  9. Pipette selon l'une des revendications 1 à 8, dans laquelle l'extrémité inférieure de la première partie de recouvrement (42) présente l'arête biseautée (70).
  10. Pipette selon la revendication 9, dans laquelle la première partie de recouvrement (42) présente une épaisseur de paroi s'affinant progressivement vers l'extrémité inférieure.
  11. Pipette selon l'une des revendications 1 à 10, dans laquelle la première partie de recouvrement (42) présente une épaisseur de paroi de 0,5 mm maximum, de préférence de 0,4 à 0,2 mm, au niveau de l'arête d'engagement.
  12. Pipette selon l'une des revendications 1 à 11, dans laquelle la première partie de recouvrement (42) présente une surface de contact plane sur le côté tourné vers la crémaillère (28), sur laquelle la crémaillère (28) peut s'appliquer et dans laquelle est disposée la fente longitudinale (61).
  13. Pipette selon les revendications 9 et 12, dans laquelle la première partie de recouvrement (42) présente un chanfrein (72) dirigé vers l'extrémité inférieure sur le côté opposé à la crémaillère (28).
  14. Pipette selon l'une des revendications 1 à 13, dans laquelle la première partie de recouvrement (42) présente la forme d'un corps creux allongé avec deux parties latérales (54, 55) en forme de bandes parallèles et une base (56), lesquelles délimitent un canal (57), la fente longitudinale (61) étant formée dans la base (56) et la deuxième partie de recouvrement (43) étant enrobée par le plastique de la première partie de recouvrement (42) dans la base (56).
  15. Pipette selon l'une des revendications 1 à 14, dans laquelle la première partie de recouvrement (42) présente des ailettes (74, 75) faisant saillie latéralement sur les deux côtés longitudinaux.
  16. Pipette selon l'une des revendications 1 à 15, dans laquelle la crémaillère (28) est moulée par injection d'un seul tenant avec l'ergot (80).
  17. Pipette selon l'une des revendications 1 à 15, dans laquelle l'ergot (80) est disposé de manière à pouvoir être ajusté sur la crémaillère (28) dans la direction axiale.
EP15732592.9A 2014-07-04 2015-06-23 Pipette servant à faire fonctionner une seringue Active EP3164214B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL15732592T PL3164214T3 (pl) 2014-07-04 2015-06-23 Pipeta do uruchamiania strzykawki

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102014109345.3A DE102014109345A1 (de) 2014-07-04 2014-07-04 Pipette zum Betätigen einer Spritze
PCT/EP2015/064076 WO2016001010A1 (fr) 2014-07-04 2015-06-23 Pipette servant à faire fonctionner une seringue

Publications (2)

Publication Number Publication Date
EP3164214A1 EP3164214A1 (fr) 2017-05-10
EP3164214B1 true EP3164214B1 (fr) 2018-09-19

Family

ID=53496656

Family Applications (1)

Application Number Title Priority Date Filing Date
EP15732592.9A Active EP3164214B1 (fr) 2014-07-04 2015-06-23 Pipette servant à faire fonctionner une seringue

Country Status (7)

Country Link
US (1) US10507463B2 (fr)
EP (1) EP3164214B1 (fr)
JP (1) JP6595518B2 (fr)
CN (1) CN106470760B (fr)
DE (1) DE102014109345A1 (fr)
PL (1) PL3164214T3 (fr)
WO (1) WO2016001010A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3335795B1 (fr) 2016-12-16 2019-03-27 Eppendorf AG Procédé de dosage de liquide au moyen d'une pipette et d'une seringue et pipette destinée à actionner une seringue pour le dosage de liquides
PL3399214T3 (pl) 2017-05-05 2021-01-25 Eppendorf Ag Elektroniczny napęd dozujący
CN107154597A (zh) * 2017-05-31 2017-09-12 成都振中电气有限公司 能改变排线槽大小的排线装置
US11471878B2 (en) * 2019-10-25 2022-10-18 Mettler-Toledo Rainin, LLC Powered positive displacement pipette
US11389792B2 (en) 2019-10-25 2022-07-19 Mettler-Toledo Rainin, LLC Syringe for powered positive displacement pipette
US11369954B2 (en) * 2019-10-25 2022-06-28 Mettler-Toledo Rainin, LLC Powered positive displacement pipette assembly
US11911767B2 (en) 2019-10-25 2024-02-27 Mettler-Toledo Rainin, LLC Positive displacement pipette syringe identification system
US11446672B2 (en) 2019-10-25 2022-09-20 Mettler-Toledo Rainin, LLC Powered positive displacement pipette syringe piston grasping mechanism
WO2021163437A1 (fr) 2020-02-14 2021-08-19 DeNovix, Inc. Pipette à plage volumétrique étendue dynamique

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3855867A (en) * 1972-08-21 1974-12-24 Oxford Lab Liquid transfer pipetting device
DE2926691C2 (de) 1979-07-02 1983-05-26 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Repetierpipette
FI58875C (fi) * 1979-08-31 1981-05-11 Suovaniemi Finnpipette Pipett
GB2109690B (en) * 1981-02-12 1985-02-20 Robert Charles Turner Dose metering plunger devices for use with syringes
US4415101A (en) * 1982-01-22 1983-11-15 Shapiro Justin J Incremental liquid dispensing device
FI831239A0 (fi) 1983-04-13 1983-04-13 Labsystems Oy Doseringspipett
DE4341229C2 (de) 1993-12-03 1995-09-07 Eppendorf Geraetebau Netheler Pipettensystem
DE4342178C2 (de) 1993-12-10 1995-09-28 Eppendorf Geraetebau Netheler Pipettensystem
DE4414760C1 (de) 1994-04-27 1995-08-24 Eppendorf Geraetebau Netheler Repetierpipette
DE9415847U1 (de) * 1994-09-30 1994-12-08 Ritter, Ralf, 86150 Augsburg Repetierpipette
DE4437716C2 (de) 1994-10-21 1996-10-17 Eppendorf Geraetebau Netheler Repetierpipette
JP2838070B2 (ja) * 1996-03-07 1998-12-16 株式会社ニチリョー 反復式ピペット
FI961649A0 (fi) * 1996-04-15 1996-04-15 Labsystems Oy Spaerrpipett
CN1248734A (zh) * 1998-09-22 2000-03-29 陈国君 拨盘式可调移液器
CN1248495A (zh) * 1998-09-22 2000-03-29 陈国君 快速可调式移液器
DE102004003433B4 (de) * 2004-01-21 2006-03-23 Eppendorf Ag Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen
MXPA06009420A (es) 2004-02-18 2007-03-23 Taica Corp Producto de transferencia hidraulica.
DE102005023203B4 (de) 2005-05-20 2009-06-04 Eppendorf Ag Pipette
DE102007042115B4 (de) 2007-09-05 2010-12-02 Eppendorf Ag Pipette
DE202010010942U1 (de) 2010-08-03 2011-11-08 Brand Gmbh + Co Kg Pipette für ein Pipettensystem mit Pipette und Spritze
DE102010047126A1 (de) * 2010-10-04 2012-04-05 Eppendorf Ag Pipette
DE102010047826A1 (de) * 2010-10-04 2012-04-05 Eppendorf Ag Elektronische Pipette
DE102012002169B4 (de) * 2012-02-07 2017-02-09 Eppendorf Ag Pipette
DE102012003846B4 (de) * 2012-02-29 2014-12-11 Eppendorf Ag Pipette
DE102012102292A1 (de) 2012-03-19 2013-08-01 Ritter Gmbh Vorrichtung zum wiederholten Dosieren mit Anzeige
US9289762B2 (en) 2012-04-23 2016-03-22 Eppendorf Ag Method for dosing a liquid using a pipette and a syringe, and pipette for operating a syringe for dosing a liquid
PL2656916T3 (pl) 2012-04-23 2019-07-31 Eppendorf Ag Sposób dozowania cieczy za pomocą pipety i strzykawki oraz pipeta do uruchamiania strzykawki do dozowania cieczy
US9579644B2 (en) * 2012-04-23 2017-02-28 Eppendorf Ag Pipette for operating a syringe
PL2656915T3 (pl) * 2012-04-23 2019-04-30 Eppendorf Ag Pipeta do uruchamiania strzykawki
DE102012011938B4 (de) * 2012-06-18 2015-02-05 Eppendorf Ag Pipette zum Betätigen einer Spritze
US20140010732A1 (en) * 2012-06-18 2014-01-09 Eppendorf Ag Pipette for Operating a Syringe

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Also Published As

Publication number Publication date
WO2016001010A1 (fr) 2016-01-07
US20170151556A1 (en) 2017-06-01
DE102014109345A1 (de) 2016-01-07
US10507463B2 (en) 2019-12-17
JP2017523029A (ja) 2017-08-17
CN106470760B (zh) 2019-06-07
CN106470760A (zh) 2017-03-01
JP6595518B2 (ja) 2019-10-23
PL3164214T3 (pl) 2019-02-28
EP3164214A1 (fr) 2017-05-10

Similar Documents

Publication Publication Date Title
EP3164214B1 (fr) Pipette servant à faire fonctionner une seringue
EP2676730B1 (fr) Pipette destinée à actionner une seringue
DE102007010299B4 (de) Handpipettiervorrichtung
EP2735369B1 (fr) Pipette multi-canal
EP1146924B1 (fr) Dispositif d'administration d'un produit injectable
DE4200595C2 (de) Baugruppe zum Infusion-Set für eine Insulinpumpe
EP2033712B1 (fr) Pipette
DE102004003433B4 (de) Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen
DE4414760C1 (de) Repetierpipette
DE69306349T2 (de) Ausgabegerät
EP2633913B1 (fr) Dispositif de pipetage et dispositif de pipetage multi-canal
EP1789117A1 (fr) Dispositif de dosage comprenant un ecoulement commande
EP2319623B1 (fr) Dispositif de dosage pour liquides et procédé de dosage de liquides
EP2656915B1 (fr) Pipette destinée à actionner une seringue
EP0033938B1 (fr) Tube à plusieurs compartiments
EP2659978B1 (fr) Pipette avec systeme de blockage
EP0679440A1 (fr) Pipette de distribution à répétition
EP2783753B1 (fr) Distributeur destiné à actionner une seringue
DE4437716C2 (de) Repetierpipette
EP3680017A1 (fr) Pipette destinée à une utilisation avec une pointe de pipette
EP3335795B1 (fr) Procédé de dosage de liquide au moyen d'une pipette et d'une seringue et pipette destinée à actionner une seringue pour le dosage de liquides
EP2415525B1 (fr) Pipette pour un système de pipetage avec pipette et seringue
EP1776566A2 (fr) Doseur multiple pour petites quantites
EP2656916B1 (fr) Procédé de dosage de liquide au moyen d'une pipette et d'une seringue ainsi que la pipette destinée à actionner une seringue pour le dosage de liquides
DE2705655C2 (fr)

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20170112

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

INTG Intention to grant announced

Effective date: 20180329

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

Free format text: NOT ENGLISH

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: DE

Ref legal event code: R082

Ref document number: 502015005986

Country of ref document: DE

REG Reference to a national code

Ref country code: CH

Ref legal event code: NV

Representative=s name: ISLER AND PEDRAZZINI AG, CH

Ref country code: AT

Ref legal event code: REF

Ref document number: 1042617

Country of ref document: AT

Kind code of ref document: T

Effective date: 20181015

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

Free format text: LANGUAGE OF EP DOCUMENT: GERMAN

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 502015005986

Country of ref document: DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20180919

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181220

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181219

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181219

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190119

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190119

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 502015005986

Country of ref document: DE

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

26N No opposition filed

Effective date: 20190620

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

REG Reference to a national code

Ref country code: BE

Ref legal event code: MM

Effective date: 20190630

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20190623

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20190623

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20190630

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20150623

REG Reference to a national code

Ref country code: AT

Ref legal event code: MM01

Ref document number: 1042617

Country of ref document: AT

Kind code of ref document: T

Effective date: 20200623

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20200623

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 8

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20180919

REG Reference to a national code

Ref country code: DE

Ref legal event code: R081

Ref document number: 502015005986

Country of ref document: DE

Owner name: EPPENDORF SE, DE

Free format text: FORMER OWNER: EPPENDORF AG, 22339 HAMBURG, DE

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230527

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20230630

Year of fee payment: 9

Ref country code: DE

Payment date: 20230620

Year of fee payment: 9

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20230622

Year of fee payment: 9

Ref country code: CH

Payment date: 20230702

Year of fee payment: 9

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20230721

Year of fee payment: 9

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: PL

Payment date: 20230616

Year of fee payment: 9