EP2676730B1 - Pipette destinée à actionner une seringue - Google Patents

Pipette destinée à actionner une seringue Download PDF

Info

Publication number
EP2676730B1
EP2676730B1 EP13003085.1A EP13003085A EP2676730B1 EP 2676730 B1 EP2676730 B1 EP 2676730B1 EP 13003085 A EP13003085 A EP 13003085A EP 2676730 B1 EP2676730 B1 EP 2676730B1
Authority
EP
European Patent Office
Prior art keywords
covering part
covering
pipette
pawl
pipette according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP13003085.1A
Other languages
German (de)
English (en)
Other versions
EP2676730A1 (fr
Inventor
Herbert Belgardt
Peter Molitor
Burkhardt Reichmuth
Jörg SATTLER
Jens Wilmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP2676730A1 publication Critical patent/EP2676730A1/fr
Application granted granted Critical
Publication of EP2676730B1 publication Critical patent/EP2676730B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0286—Ergonomic aspects, e.g. form or arrangement of controls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0234—Repeating pipettes, i.e. for dispensing multiple doses from a single charge

Definitions

  • the invention relates to a pipette for actuating a syringe.
  • the pipettes used here for actuating a syringe serve to dispense liquid received in the syringe in several steps. They are also referred to as dispensers or repeating pipettes. These pipettes have at the lower end of a rod-shaped housing a receptacle for a flange of a cylinder of the syringe and in the housing a displaceable receiving body with a piston receptacle for the upper end portion of a piston rod of a piston of the syringe.
  • the syringe can be used with the flange and the end portion of the piston rod through axially directed openings of the receptacles.
  • the flange and the end portion are held in the receptacles by means for detachable holding, which are designed, for example, as spring-loaded gripping levers.
  • the pipette comprises means for displacing the receiving body, which allow a partial withdrawal of the piston from the cylinder for the suction of liquid into the syringe and a stepwise depression of the piston into the cylinder for the stepwise discharge of liquid.
  • the DE 29 26 691 C2 and the US 4,406,170 A describe means for displacing the receiving body in the housing. These include an elevator lever connected to the receiving body and projecting out of the housing through a straight-line slot for drawing fluid into the syringe by displacing the receiving body away from the receptacle. In addition, they include a rack-pawl means for gradually advancing the piston by a reciprocating metering lever. At the metering a pivoting pawl is mounted. The rack is connected to the receiving body and arranged in the pivoting range of the pawl. An adjustable sliding cover covers the row of teeth on the rack more or less to limit the engagement of the pawl in the rack when pivoting the metering lever.
  • the sliding cover is displaceable by means of a rotary knob, which is arranged on the pipette housing.
  • the knob is designed with an eccentric guide curve in which engages a guide pin of the sliding cover.
  • the rack is designed with a lofty approach, through which in the advanced position of the piston, the cover of the rack is displaced away, so that it prevents the pawl from engaging in the uncovered teeth of the rack. This Resthubsperre prevents that a residual amount is discharged from the syringe, which is smaller than the dispensed at each dosing dose (dispensing volume).
  • the EP 0 657 216 B1 and US 5,620,661 , A describes such a pipette with a sensor for sensing protrusions and depressions on the syringe flange of the syringes and correspondingly configured syringes.
  • the sensor is used to determine the size of the syringe used.
  • electronics determines the amount of fluid dispensed at each dispensing step. This is displayed on a display.
  • a repeating pipette has a constant stepper, which is the width of the first step to move of the receiving body for the operating portion of the syringe plunger for cylinder intake for the syringe cylinder sets to a constant value, which is independent of the setting of the subsequent step sizes.
  • the cover has an elongated sleeve made of plastic, in which a threaded rod is displaceably arranged.
  • a metal sleeve is glued in the lower end of the plastic sleeve.
  • the metal sleeve protrudes from the lower end of the plastic sleeve and has an incidence edge below, below which the thread is exposed for engaging the pawl.
  • the pawl first slides over the metal sleeve, which prevents the pawl from engaging in the thread of the threaded rod. As soon as the pawl passes the incidence edge during the dosing step, it falls into the thread.
  • the pawl meets a flank or the apex of the thread, which is arranged below the thread root, too little part of the pivoting movement of the metering lever for used the displacement of the threaded rod and thus the piston. Also in this case too little liquid is dispensed. It may even happen that the pawl slips off the flank or the apex of the thread and falls further down into the thread root or several times slipped over the thread, which leads to an increased dosing error or no liquid is discharged.
  • the dosing error can be overcome by adjusting the threaded rod.
  • the threaded rod is screwed into a setting thread in the receiving body for the upper end of the piston rod and provided with a transverse bore.
  • the adjustment of the volume of liquid to be dispensed at each step is also carried out by means of a rotary knob.
  • the engagement of the pawl in the rack by means of the knob is adjustable.
  • the knob on two knob parts whose angular position is adjustable to each other via a transmission.
  • a rotary knob part has markings for adjusting the dosing volume.
  • the other knob part has the guide cam engaged by the pin projecting from the cover. Turn the knob to move the cover along the rack.
  • the present invention seeks to provide a pipette, in which with reduced effort an optimal collapse of the pawl can be achieved in the rack.
  • the pipette according to the invention is based on the finding that a non-optimal collapse of the pawl in the toothing of the rack is significantly based on the manufacturing tolerance of the cover, which is arranged on the rack and limits the engagement of the pawl in the teeth.
  • Decisive for the error is the distance of the incidence edge of the pin or another coupling element, via which the cover is coupled to the dial. Therefore, according to the invention, the cover has a first cover part, on which the pin or another coupling element for coupling with a dial or another adjustment element is arranged, and a second cover part, which has the incidence edge. The first and the second cover part are displaceable relative to each other in the longitudinal direction of the rack.
  • the cover comprises means for positioning, which make it possible to fix the first and the second cover part in a certain setting with respect to each other.
  • the means for positioning thus ensure that the distance of the incidence edge from the coupling element is not changed unintentionally.
  • the adjustment of the first and second cover parts relative to each other and the determination of the first and second cover parts in the set position can basically be done before installing the cover in the pipette. It is sufficient to adjust the cover so that a predetermined distance between the incidence edge and the coupling element is maintained. This adjustment of the cover is easily carried out by hand and automated.
  • the receiving body is displaced between an upper position and a lower position.
  • the upper position is determined by an upper stop at a fixed position in the housing, against which the first means for displacing the receiving body or the receiving body itself meet at the end of the displacement in the housing of the recording away.
  • the lower position is determined by a lower stop at a fixed position in the housing against which the second means for displacing the receiving body or the receiving body itself meet at the end of the displacement of the receiving body for the first recording.
  • the adjustment of the cover is preferably carried out in the upper position of the receiving body, the starting position of the receiving body immediately before dispensing.
  • the first cover part is arranged on the toothed rack and the second cover part is held on the first cover part.
  • the second cover part is arranged on the side facing the pawl of the first cover part.
  • the first cover part has the coupling element and the second cover part has the incidence edge.
  • the first cover part is a carrier for the second cover part.
  • the second cover part is arranged on the toothed rack and the first cover part is held on the second cover part.
  • the first cover part is on the Pawl facing side of the second cover part arranged.
  • the second cover part has the incidence edge and the first cover part has the coupling element. By displacing the first cover part on the second cover part, the distance between the incidence edge and the coupling element is adjustable.
  • the cover has means for axially guiding the second with respect to the first cover part and the first and the second cover part are guided by the means for axially guiding parallel to the rack.
  • the means for axially guiding the second cover part on the edge overarching guide structures of the first cover part is slidable during assembly under the guide structures or snaps under this. Thereafter, the second cover part is secured to the first cover part. As a result, the assembly is facilitated and simultaneously achieved the axial guidance parallel to the rack.
  • the guide structures are preferably designed as a guide hook.
  • the second cover part is a thin-walled metal part, preferably a metal sheet or a metal sleeve.
  • the pawl can be guided at a short distance from the rack, so that it falls directly into the toothing after passing the incidence edge.
  • the first cover part is a guided on the rack hollow body and / or the second cover part strip-shaped.
  • the first Cover member forms a stable support for the second cover part.
  • the hollow body can be manufactured with low error tolerances of plastic, in particular by injection molding.
  • the first cover part is preferably box-shaped with a channel on one side, which receives the rack.
  • the strip-shaped, second cover part can be produced from a sheet metal. In the execution of the second cover part made of metal, the distance between the pawl and toothing and the friction between the pawl and cover can be kept particularly low.
  • the second cover part is resilient. A resilient design of the second cover part favors the achievement of a Resthubsperre which prevents the discharge of a residual amount of liquid from the syringe, if this is less than the set dosage.
  • the rack on the side facing the cover on a protruding cam the first cover part has a longitudinal slot, from which the cam in the displacement of the receiving body for the second attachment portion of the syringe to the first receptacle for the first attachment portion of the syringe towards the outlet and the second cover part has an angled portion and a spring tongue connected therewith, on which the cam strikes the first receptacle at the end of the displacement of the receiving body, whereby the spring tongue is deflected and the latch is prevented from further engagement with the rack by the spring tongue becomes.
  • the second cover part is preferably formed overall as a thin-walled metal part, preferably made of sheet metal. This is advantageous for the formation of the bend and the spring tongue as integral parts of the metal part and for holding the pawl at only a small distance from the teeth. Dimensional tolerances of the first cover part and the Second cover part and tolerances due to the assembly of the first and the second cover part are advantageously compensated by the adjustability of the relative position of the first and second cover part according to the invention.
  • the means for positioning on means for adjusting the position of the second cover part relative to the first cover part in the longitudinal direction of the rack.
  • the means for positioning can be used in this embodiment for the adjustment of the second cover part relative to the first cover part.
  • the means for positioning an eccentric part with a shaft, an eccentric on the shaft and a tool engagement at one end of the shaft wherein the shaft is mounted at one end in a bearing bore of the first cover part or the second cover part and the Exzenter engages in an eccentric receiving the second cover part or the first cover part, so that by rotating the shaft via the eccentric, the second cover part is displaceable relative to the first cover part.
  • This configuration makes it possible to adjust the relative position of the first and second cover parts by simply rotating the shaft.
  • the shaft can be rotated by attaching a tool to the tool bit and turning the tool. In the set position, the first and second cover parts are secured by the means for positioning.
  • the shaft has a friction with respect to the first and / or the second cover part, which prevents the shaft from rotating inadvertently.
  • a disc is arranged at one end of the shaft, which rests on the outside of the second cover part or the first cover part and / or the shaft has at one end a latching projection which has an edge of the bearing bore of the first cover part or of the second Cover part engages behind.
  • the locking projection can be rotated behind the edge or locked behind it.
  • the means for positioning comprise a first holder on the first cover part and a second holder on the second cover part, a parallel to the rack bore or a rack parallel to the other adjustment thread in the first holder and a rack parallel to the further adjustment thread or a rack parallel bore in the second holder, one screwed into the bore of the first or second holder and in the further adjustment thread of the second or first holder further adjusting screw and another spring element between the first cover part and second cover part, which is effective against the screwing of the other adjusting screw
  • the further spring element is a helical spring which is guided on the further adjusting screw and is supported at one end on the first holder and at the other end on the second holder.
  • the cover parts By screwing the other adjustment against the action of the other spring element, the cover parts can be displaced relative to each other in one direction and by unscrewing the other adjusting screw and the action of the other spring element they are displaced in the other direction relative to each other.
  • the means for positioning enable adjusting the position of the second cover part with respect to the first cover part in an adjustment range corresponding to the distance between two valleys on different sides of a tooth of the toothing. This makes it possible to set the distance of the incidence edge of the coupling element exactly so that the pawl optimally falls into the toothing when passing the incidence edge.
  • the means for positioning enable adjusting the position of the second cover part with respect to the first cover part in an adjustment range that is +/- 0.25 mm.
  • This adjustment range is adjusted on a toothed rack at a distance from tooth to tooth or from valley to valley of 0.5 mm. This pitch of the rack has been found to be advantageous for adjusting dosing levels that are often required in the laboratory.
  • the adjustment range makes it possible to adjust the first and second cover parts always so that the pawl optimally falls into the teeth.
  • These means are, for example, means for clamping the first cover part on the second cover part.
  • These may for example be formed by a screw which is screwed into a threaded bore of the first cover part and engages in a slot of the second cover part, which is aligned parallel to the rack. When the screw is released, the relative position of the first and second cover part can be adjusted. By tightening the screw, the first and second cover parts are clamped together so that they maintain the set relative position.
  • Screws of the aforementioned type can also form the axial guidance of the two cover parts in the longitudinal direction of the rack.
  • the coupling element is a protruding from the first cover part pin and the adjusting element has a guide curve on the underside, in which engages the pin, so that upon adjustment of the adjusting element, the guide cam moves the pin in the axial direction of the rack.
  • the adjusting element is a dial.
  • the guide curve is spiral.
  • the means for positioning are arranged on the cover so that they are not covered by the adjusting element, at least in one setting of the adjusting element. This favors an adjustment of the relative position of the first and second cover parts also in the assembled state of the pipette. If necessary. the adjusting element must be set only in a position in which means for positioning are not covered by the adjustment and accessible through a housing opening or after removal of a housing part.
  • the means for positioning are arranged at the upper end of the cover. This embodiment is particularly advantageous for adjusting the relative position of the first and second cover parts with the pipette assembled.
  • the pawl on a plurality of ratchet teeth, which engage in the toothing of the rack after passing the incidence edge.
  • the invention also includes embodiments in which the pawl has only a single pawl tooth.
  • the cam on the side of the Abhalteelements protrudes through a slot of the rack and has an axial bore
  • an adjusting screw is screwed through the bore into a Einstellgewinde at the upper end of the rack and is a spring element between the upper end of the Rack and arranged the cam.
  • the first cover part made of plastic and / or the second cover part is made of a metal sheet.
  • the first cover part is preferably produced by injection molding and / or the second cover part by etching or stamping and, if necessary, bending a sheet or other manufacturing methods.
  • the means for releasably holding the first attachment portion in the first receptacle and the second attachment portion in the second receptacle are preferably formed as in EP 0 656 229 B1 and US 5,620,660 A or in the EP 1 724 120 B1 and the US 7,731,908 B2 described, the contents of which are hereby incorporated into the present application.
  • the first means for displacing the receiving body in the housing are preferably designed as an elevator lever, as in the DE 29 26 691 C2 and the US 4,406,170A or the publications cited in the preceding paragraph, the contents of which are hereby incorporated into the present application.
  • the pipette has a sensor for scanning elevations and depressions on the syringe flange of the syringes, as in US Pat EP 0 657 216 B1 and the US 5,620,661A described, the contents of which are hereby incorporated into the present application.
  • the term "rack" both a rack in the true sense, d. H. a rod with a series of teeth arranged thereon, as well as a threaded rod, d. H. a rod with at least one thread arranged thereon, addressed.
  • the pipette according to the invention is provided with a rack in the true sense.
  • top and bottom refer to the orientation of the pipette in which the bar-shaped housing is vertically aligned and the syringe receptacle is located below ,
  • a pipette 1 has a rod-shaped housing 2 in which a syringe 3 is held at the bottom. From a side wall of the housing 2 is above a straight line slot 4 an elevator lever 5 of the housing 2 in front. From the same side wall of the housing 2 is about two further slots 6, 7, a control knob 8 of a rack-pawl control before.
  • a display device in the form of a display 9 is embedded in the same side wall of the housing 2. From openings in the adjacent side wall are segments of a dial 10 out.
  • the syringe 3 has a cylinder 11 and a piston 12 arranged displaceably therein.
  • the cylinder 11 has at the bottom a conical portion 13 with a hole 14 for the passage of liquids and above a cylindrical portion 15 in which the piston 12 is displaceable.
  • the cylinder 11 has a first mounting portion 16 with a circumferential flange 17.
  • From the piston 12 is upwardly a piston rod 18 which has a second mounting portion 19 with a plurality of circumferential beads.
  • the syringe 3 is arranged with the flange 17 in a first receptacle 20 at the lower end of the housing 2, which at the lower end of the housing 2 has an axially directed first opening 21 for insertion and removal of the syringe 3.
  • the syringe 3 presses with its upper side against a pressure-sensitive ring sensor 22, which scans projections on the upper edge of the flange 17.
  • the indicated on the flange 17 code denotes the size of the respective syringe 3.
  • the flange 17 is held in this position by means of first means for releasably holding in the form of first gripping levers 23 in the housing 2.
  • the second attachment portion 19 of the piston 12 is arranged in a second receptacle 24 in a hollow cylindrical receiving body 25. This has for insertion of the second attachment portion 19 on an axially directed second opening 26.
  • the second attachment portion 19 is by means of second means for releasably holding held in the form of second gripping levers 27 which engage between the beads of the second mounting portion 19 or pinch this.
  • the receiving body 25 is fixedly connected to a rack 28 with a toothing 29 which extends below the slot 4 in the longitudinal direction of the housing 2.
  • An elevator lever holder 30 is fixed to the receiving body 25 and to a lower part of the rack 28.
  • an elevator lever carrier 31 is present, which abuts with a sliding plate 32 at the bottom of the edges of the slot 4.
  • the elevator lever carrier 31 has an upwardly projecting post 33, which passes through the slot 4.
  • the elevator lever 5 is fixed outside the housing 2.
  • an actuating element in the form of a metering lever 36 is pivotably mounted in a swivel bearing 34 in a bulge 35 of the slot 4 opposite side wall of the housing 2.
  • the metering lever 36 has according to Fig. 3 to 6 two spaced legs 37, 38 which protrude on the opposite side wall of the housing 2 from the two slots 6, 7. There, the control knob 8 is fixed to the protruding ends of the legs 37, 38.
  • the elevator lever carrier 31 strikes a fixedly arranged in the housing 2 bearing body in which the pivot bearing 34 is formed for the metering lever 36. As a result, the upper stop for the displacement of the receiving body 25 is given.
  • a pawl 39 is pivotally mounted between the two legs 37, 38 of the metering lever 36.
  • the pawl 39 is arranged with a plurality of ratchet teeth 40 (three in the example) above the toothing 29 of the rack 28.
  • the metering lever 36 is moved from a spring device to the position of Fig. 2 pressed. Contrary to the action of the spring device of the metering lever 36 by pressing the control knob 8 is pivoted downwards.
  • the pawl 39 is pressed by means of a further spring means to the toothing 29 of the rack 28. As a result, the rack 28 is pushed down.
  • a sliding cover 41 is arranged between the pawl 39 and the rack 28 .
  • the cover 41 is displaceable by turning the laterally projecting out of the housing 2 dial 10 so that it covers the teeth 29 of the rack 28 more or less.
  • the pawl is first pressed against the cover 41 upon actuation of the control knob 8 and falls under it into the toothing 29 a. Therefore, the amount of displacement of the rack bar 28 when pivoting the metering lever 36 downward depends on the position of the cover 41 with respect to the toothing 29.
  • the cover 41 has according to Fig. 7 to 10 a first cover part 42 in the form of an elongated hollow body.
  • the first cover part 42 has two parallel, strip-shaped side parts 43, 44. These are bridged at the front edges in the upper region by a plate-shaped bridging member 45. Perpendicular to the outer sides of the side parts 43, 44 are strip-shaped wing parts 46, 47 before.
  • a coupling element in the form of a pin 48 is present.
  • a plate-shaped sliding block 49 is present.
  • the bridging part has a bearing bore 50 which extends perpendicularly through the bridging part 45.
  • the bearing bore 50 is formed in a portion of the bridging member 45 which projects beyond the side member 44.
  • the bearing bore 50 has a front recess 51 at the front and a rear recess 52 at the bottom. In between runs in the bearing bore 50, a circular disk-shaped bore edge 53 to.
  • the bridging part 45 has a first recess 54 at an upper end, and the bearing hole 50 is opened toward the recess 54.
  • a longitudinal groove 57 which extends in the longitudinal direction of the first cover part and is opened to a channel 58 in the distance region between the side parts 43, 44.
  • a longitudinal slot 59 is present between the front edges of the side parts 43, 44.
  • the first cover part 42 is preferably injection molded in one piece from plastic.
  • the cover 41 has a second cover part 60, which is substantially strip-shaped.
  • the second cover part 60 has at the top a plate-shaped head section 61 and at the bottom two parallel strip-shaped middle sections 62, 63, which are connected via opposite bends 64, 65 to the head section 61.
  • the middle portion 62 has at the lower end a U-shaped foot portion 66.
  • the foot portion 66 is connected at one leg to the central portion 62 and the other leg is aligned with the central portion 63.
  • the central portion 63 is connected at the lower end via a first connecting portion 67 with a central strip portion 68 having a protruding bend 69 at the upper end.
  • the middle strip portion 68 is disposed between the legs of the U-shaped foot portion 66.
  • central portion 63 is connected at the lower end via a second connecting portion 72 with a spring tongue 73 which is disposed adjacent to the U-shaped foot portion 66 and extending parallel to the central portions 62, 63 downwardly.
  • the head portion 61 has a large hole 74 which receives the pin 48 with play. Further, the head portion 61 has a large rectangular cutout 75, which receives the sliding block 49 with axial play.
  • the two middle sections 62, 63 have free spaces 80, 81 at the edges facing away from one another between projecting tongues 76 to 79.
  • the guide hooks 55, 56 engage with axial play in the free spaces 80, 81 and overlap the middle sections 62, 63 at the edge.
  • the second cover part 60 is held on the first cover part 42, guided in the longitudinal direction on the first cover part 42 and displaceable in the longitudinal direction relative to the first cover part 42.
  • the head section 61 also has an eccentric receptacle 82 at an upper corner.
  • the eccentric 82 is approximately rectangular, with the corners are rounded.
  • the eccentric 82 is disposed above the bearing bore 50, wherein the shorter side of the Exzenter matter 82 is arranged in the longitudinal direction of the first cover member 42 and the longer side perpendicular thereto.
  • the second cover part 60 is for example made in one piece from a sheet of spring steel by punching and bending.
  • the cover 41 includes an eccentric 83.
  • the eccentric 83 has according to FIGS. 11 and 12 a shaft 84 on which an eccentric 85 is arranged.
  • the diameter of the eccentric 85 corresponds to the length of the shorter side of the Exzenterfact 82.
  • a tool engagement 86 eg Torx, Allen, Phillips or slot
  • the shaft 84 has a radially projecting latching projection 88 in the form of a circular disk segment.
  • the disc 87 and the eccentric 85 have manufacturing reasons, a second recess 89, the radial boundaries with the locking projection 88 are aligned.
  • the eccentric part 83 is inserted in the axial direction of the bearing bore in the eccentric receptacle 82 and the bearing bore 50, so that the disc 87 engages over the second cover part 60.
  • the latching projection 88 is inserted through the first recess 54 at the corner of the bridging member 45 and rotated below the bore rim 53.
  • the eccentric 85 is arranged in the region of the eccentric receiver 82 and the front recess 51.
  • the latching projection 88 engages under the bore edge 53 and engages in the rear recess 52 a.
  • the eccentric 83 is rotatable by attaching a tool to the tool engagement 86.
  • the eccentric 85 displaces the second cover part 60 with respect to the first cover part 42 in the axial direction.
  • FIGS. 13 and 15 For example, the two extreme positions of the second cover part 60 with respect to the first cover part 42 are shown at this displacement, which from the middle position in Fig. 14 are about +/- 0.25 mm away.
  • the rack 28 extends through the channel 58 of the first cover 42.
  • the pawl 39 is threaded with a guide slot 90 at the lower end on the spring tongue 73 of the second cover member 60.
  • the cover 41 is slidably guided in the housing in the longitudinal direction.
  • the dial 10 according to Fig. 16 a central bearing hole 91 and a spiral guide cam 92.
  • the dial 10 is rotatably supported on the bearing hole 91 on an axis on the housing 2 and the pin 48 of the cover 41 engages the guide cam 92 a.
  • the cover 41 is displaced along its guide in the housing 2.
  • the dial 10 a further sensor 93 is associated, which detects the rotational position of the dial 10.
  • a cam 94 is arranged at the upper end of the rack 28 at the outside.
  • the cam 94 protrudes out of a further longitudinal slot 95 of the rack 28 to the outside.
  • the cam 94 has an axial bore 96.
  • An adjusting screw 97 is screwed through the axial bore 96 into a Einstellgewinde 98 at the upper end of the rack 28.
  • a coil spring 99 is guided on the adjusting screw 97 and is supported at one end on the screw head of the adjusting screw 97 and the other end at the upper end of the rack 28 to hold the cam 94 in the system on the screw head.
  • a transfer member 100 is arranged for controlling a Umledgehubes in the housing of the pipette.
  • the transmission member 100 has a strip-shaped transmission section 101, which is guided parallel to the rack 28 in the housing 2 of the pipette and displaced.
  • the strip-shaped transfer section 101 has a lower transfer member end 102, which is located at a displacement of the transfer member 101 down in the displacement region of a side of the rack 28 projecting lower stop 103.
  • control arms 104, 105 are connected, which are arranged parallel to each other in a plane perpendicular to the plane of the drawing.
  • the transmission member 100 is displaced so that the control arms 104, 105 reach with their upper ends in the displacement region of bevelled stop edges on the legs 37, 38 (see. Fig. 10 to 13 ).
  • the transfer member and the cooperating components are preferably formed as in the European patent application 120 02 849.3-2113 and in the U.S. Provisional Application No. 61 / 636,977 described, the contents of which are hereby incorporated into the present application.
  • a circuit board 106 is arranged with an electronics 107.
  • an electrical power supply in the form of batteries or rechargeable batteries 108.
  • the electronics 107 is connected to the ring sensor 22, the further sensor 93 for detecting the rotational position of Dial 10 and the display 9 wired. Furthermore, the electronics 107 is connected to the battery 108.
  • the electronics 107 determined from the measurement signals supplied by the ring sensor 22, the respective syringe size and the setting of the dial 10, the respective step size. From this it calculates the set dispensing volume and brings it to display 9 for display.
  • a syringe 3 is first connected with a user-selected syringe size releasably connected to the pipette 1 by being inserted with the first mounting portion 16 in the receptacle 20 and with the second mounting portion 19 in the receptacle 24, so that the Flange 17 of the first gripping levers 23 and the attachment portion 19 is gripped by the second gripping levers 27.
  • This situation is in Fig. 2 shown.
  • the ring sensor 22 scans the coding on the flange 17 of the syringe 3.
  • the electronics 107 determines based on the signals supplied by the ring sensor 22 that a syringe 3 is inserted and turns on the display 9.
  • the electronics 107 determined based on the signals supplied by the ring sensor 22 and the sensor 93 from the set Dispensiervolumen and brings this on the display 9 for display.
  • the user changes the setting of the Dispensiervolumens means of the dial 10 and the changed Dosiervolumen is displayed by the display 9.
  • the elevator lever 5 For drawing liquid through the hole 14 of the syringe 3, the elevator lever 5 is moved out of the position of Fig. 2 pushed up.
  • a reverse stroke must be carried out. This is done by pressing the control knob 8. Here meet the legs 37, 38 on the control arms 104, 105 and move the transmission member 100 down. The transmission member 100 takes over the lower stop 103 with the rack 28. This reverse stroke removes the lots from the system.
  • the displacement of the rack 28 depends on each pivoting of the metering lever 36 to reach a lower stop of a set by means of the dial 10 position of the cover 41 from. After relieving the control knob 8 he is pushed by the spring device up and another dosing can be performed.
  • Residual liquid in the syringe 3 can be discharged by displacing the elevator lever 5 down. Thereafter, the syringe 3 can be separated from the pipette 1.
  • the user operates the first gripping levers 23, which act on projections on the inside of the second handle lever 27, as in the EP 0 656 229 B1 and the US 5,620,660 A described.
  • a pipette according to the invention instead of directly manually operable gripping levers 23, 27, first and second means for releasably holding the syringe, which are formed as in the EP 2 033 712 A1 and US 2009/139351 A1 described, the contents of which are hereby incorporated into the present application.
  • the syringe is released after emptying by further actuation of the metering lever of the pipette.
  • the pipette on a transmission which is controlled by the metering lever and acts on the first and second means for holding the syringe to release the syringe from the pipette.
  • the first and second releasably holding means are designed as gripping levers which are integrated into the housing and can not be actuated directly from the outside.
  • each ratchet tooth engages exactly in a valley between two teeth of the teeth.
  • Ratchet teeth 40 are unfavorably aligned with the teeth 29, so that they can come in when pivoting the metering lever 36 in the wrong position or slipping over the teeth 29.
  • Adjusting the cover 41 in the basis of Fig. 13 to 15 this can be overcome, so that the pawl 39 is incident in the toothing 29, as in the Fig. 18 and 19 shown.
  • the adjustment is possible through a hole in the housing 2 or after removing the front housing half by applying a tool to the eccentric 83 (see. Fig. 3 ).

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)

Claims (17)

  1. Pipette destinée à actionner une seringue, avec
    • un boîtier (2) en forme de tige,
    • un premier logement (20) avec une première ouverture (21) à l'extrémité inférieure du boîtier (2) pour l'insertion d'un premier segment de fixation (16) sur le bord supérieur d'un cylindre (11) de la seringue (3),
    • un corps de réception (25) avec un deuxième logement (24) et une deuxième ouverture (26) à l'extrémité inférieure dans le boîtier (2) pour l'insertion d'un deuxième segment de fixation (19) sur un piston (12) de la seringue (3),
    • des premiers moyens pour la retenue (23) détachable du premier segment de fixation (16) dans le premier logement (20),
    • des deuxièmes moyens pour la retenue (27) détachable du deuxième segment de fixation (19) dans le deuxième logement (24),
    • des premiers moyens pour le déplacement (5) du corps de réception (25) dans le boîtier (2) pour l'éloigner du premier logement (20),
    • des deuxièmes moyens pour le déplacement (28, 36, 39) par pas du corps de réception (25) en direction du premier logement (20), avec une largeur de pas correspondant au volume de liquide à expulser de la seringue (3) pour les pas,
    • un élément d'actionnement (36) pouvant être actionné à l'extérieur du boîtier (2) pour la réalisation de pas individuels,
    • une crémaillère (28) raccordée au corps de réception (25), avec une denture (29) dans le boîtier (2),
    • un loquet (39), supporté en pivotement dans le boîtier (2) sur l'élément d'actionnement (36), qui, lors du déplacement de l'élément d'actionnement (36) vers le bas, entre en prise avec la denture (29) de la crémaillère (28) et entraîne celle-ci, et qui se dégage de la denture (29) lors du déplacement de l'élément d'actionnement (36) vers le haut,
    • un élément de réglage (10), réglable à l'extérieur du boîtier (2), pour le réglage de la largeur de pas des pas,
    • un recouvrement (41) disposé de façon déplaçable sur la crémaillère (28) sur le côté du loquet (39), avec une surface d'écartement (70) tournée vers le loquet (39) pour empêcher le loquet (39) d'entrer en prise dans la denture (29) et avec une arête de chute (71) à l'extrémité inférieure, au-dessous de laquelle la denture (29) est exposée pour la mise en prise par le loquet (39),
    • un élément d'accouplement (48) disposé sur le recouvrement (41), qui est couplé à l'élément de réglage (10) pour appliquer un réglage de l'élément de réglage (10) au recouvrement (41),
    • le recouvrement (41) présentant une première partie de recouvrement (42) sur laquelle est disposé l'élément d'accouplement (48), et une deuxième partie de recouvrement (60) sur laquelle est constituée l'arête de chute (71) dont la position relative l'une par rapport à l'autre est réglable dans la direction longitudinale de la crémaillère (28), et
    • des moyens de positionnement (83), disposés sur le recouvrement (41), qui immobilisent dans une position réglée l'une par rapport à l'autre la première partie de recouvrement et la deuxième partie de recouvrement (42, 60).
  2. Pipette selon la revendication 1, dans laquelle la première partie de recouvrement (42) est disposée sur la crémaillère (28) et la deuxième partie de recouvrement (60) est retenue sur la première partie de recouvrement (42).
  3. Pipette selon la revendication 1 ou 2, dans laquelle le recouvrement (41) présente des moyens pour le guidage axial (49, 55, 56) de la deuxième partie de recouvrement (60) par rapport à la première partie de recouvrement (42) parallèlement à la crémaillère (28).
  4. Pipette selon la revendication 3, dans laquelle les moyens axiaux de guidage (55, 56) présentent des structures de guidage de la première partie de recouvrement (42) qui recouvrent en bordure la deuxième partie de recouvrement (60).
  5. Pipette selon une des revendications 1 à 4, dans laquelle la première partie de recouvrement (42) est un corps creux guidé sur la crémaillère (28) et/ou la deuxième partie de recouvrement (60) est en forme de bande.
  6. Pipette selon les revendications 1 à 5, dans laquelle la deuxième partie de recouvrement (60) est une partie métallique à paroi mince.
  7. Pipette selon une des revendications 1 à 6, dans laquelle la crémaillère (28) présente sur le côté tourné vers le revêtement une came (94) en saillie, la première partie de recouvrement (42) présente une fente longitudinale (95) d'où sort la came (94) lors du déplacement du corps de réception (25) vers le premier logement (20), la deuxième partie de recouvrement (60) présente une partie repliée (69) et une languette élastique (73) qui y est raccordée, que vient heurter la came (94) à la fin du déplacement du corps de réception (25) vers le premier logement (20), ce qui provoque la déviation de la languette élastique (73) et ce qui empêche le loquet (39) de la languette élastique (73) d'entrer en prise davantage dans la denture (29) de la crémaillère (28).
  8. Pipette selon la revendication 7, dans laquelle la came (94), sur le côté du recouvrement (41), fait saillie hors de la crémaillère (28) à travers une autre fente longitudinale (95) et présente un alésage (96) axial, une vis de réglage (97) est vissée à travers l'alésage (96) dans un filet de réglage (98) à l'extrémité supérieure de la crémaillère (28), et un élément élastique (99) est disposé entre l'extrémité supérieure de la crémaillère (28) et la came (94).
  9. Pipette selon une des revendications 1 à 8, dans laquelle les moyens de positionnement (83) présentent des moyens de réglage de la position de la deuxième partie de recouvrement (60) par rapport à la première partie de recouvrement (42) dans la direction longitudinale de la crémaillère (28).
  10. Pipette selon une des revendications 1 à 9, dans laquelle les moyens de positionnement (83) présentent une pièce excentrique avec un arbre (84), avec un excentrique (85) sur l'arbre (84) et avec une prise d'outil (86) à une extrémité de l'arbre (84), l'arbre (84) étant, à une extrémité, supporté dans un alésage d'assise (50) de la première partie de recouvrement (42) ou de la deuxième partie de recouvrement (60), et l'excentrique (85) engrenant dans un logement d'excentrique (82) de la deuxième partie de recouvrement (60) ou de la première partie de recouvrement (42) de telle sorte que, par la rotation de l'arbre (84) par le biais de l'excentrique (85), la deuxième partie de recouvrement (60) peut être déplacée par rapport à la première partie de recouvrement (42).
  11. Pipette selon la revendication 9, dans laquelle, à une extrémité de l'arbre (84), il est disposé un disque (87) qui est plaqué sur le côté extérieur de la deuxième partie de recouvrement (60) ou de la première partie de recouvrement (42), et/ou dans laquelle l'arbre (84) présente à une extrémité une saillie crantée (88) qui agrippe par l'arrière un bord d'alésage (53) de l'alésage d'assise (50) de la première partie de recouvrement (42) ou de la deuxième partie de recouvrement (60).
  12. Pipette selon une des revendications 9 à 11, dans laquelle les moyens de positionnement (83) permettent un réglage de la position de la deuxième partie de recouvrement (60) par rapport à la première partie de recouvrement (42) dans une plage de réglage qui correspond à l'espacement de deux creux sur différents côtés d'une dent de la denture.
  13. Pipette selon une des revendications 9 à 12, dans laquelle les moyens de positionnement (83) permettent un réglage de la position de la deuxième partie de recouvrement (60) par rapport à la première partie de recouvrement (42) dans une plage de réglage qui est de +/- 0,25 mm.
  14. Pipette selon une des revendications 1 à 13, dans laquelle l'élément d'accouplement (48) est une broche qui dépasse de la première partie de recouvrement (42), et l'élément de réglage (10) présente une courbe de guidage (92) sur le côté inférieur dans laquelle la broche engrène de telle sorte que, lors d'un réglage de l'élément de réglage (10), la courbe de guidage (92) déplace la broche dans la direction axiale de la crémaillère (28).
  15. Pipette selon une des revendications 1 à 14, dans laquelle les moyens de positionnement (83) sont disposés sur le recouvrement (41) de telle sorte que, au moins dans un réglage de l'élément de réglage (10), ils ne sont pas recouverts par l'élément de réglage (10).
  16. Pipette selon une des revendications 1 à 15, dans laquelle les moyens de positionnement (83) sont disposés à l'extrémité supérieure du recouvrement (41).
  17. Pipette selon une des revendications 1 à 16, dans laquelle la première partie de recouvrement (42) est réalisée en matière plastique et/ou la deuxième partie de recouvrement (60) est réalisée en tôle.
EP13003085.1A 2012-06-18 2013-06-17 Pipette destinée à actionner une seringue Active EP2676730B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261661018P 2012-06-18 2012-06-18
DE201210011938 DE102012011938B4 (de) 2012-06-18 2012-06-18 Pipette zum Betätigen einer Spritze

Publications (2)

Publication Number Publication Date
EP2676730A1 EP2676730A1 (fr) 2013-12-25
EP2676730B1 true EP2676730B1 (fr) 2015-08-12

Family

ID=48703101

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13003085.1A Active EP2676730B1 (fr) 2012-06-18 2013-06-17 Pipette destinée à actionner une seringue

Country Status (4)

Country Link
EP (1) EP2676730B1 (fr)
JP (1) JP2014036952A (fr)
CN (1) CN103506173A (fr)
DE (1) DE102012011938B4 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102014109345A1 (de) * 2014-07-04 2016-01-07 Eppendorf Ag Pipette zum Betätigen einer Spritze
DE102016121814A1 (de) * 2016-11-14 2018-05-17 Ika-Werke Gmbh & Co. Kg Handdosiervorrichtung
FR3080050B1 (fr) * 2018-04-17 2020-03-27 Gilson Sas Dispositif de verrouillage d'une vis de reglage de volume pour un systeme de pipetage
EP3560596A1 (fr) * 2018-04-23 2019-10-30 Eppendorf AG Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents
CN109665291B (zh) * 2019-01-31 2023-10-20 长春东睦富奥新材料有限公司 一种活塞本体正反面检查用可调机构
FI3932557T3 (fi) * 2020-07-02 2023-12-28 Eppendorf Se Monikanavainen pipettipää
CN111957370A (zh) * 2020-08-11 2020-11-20 程程 一种检验科用器皿端盖取放方法
CN114247492B (zh) * 2021-12-23 2023-02-07 安徽阜邦生物科技有限公司 一种化工检验用可快速固定的检测设备及其使用方法
CN115501420B (zh) * 2022-10-21 2023-12-22 苏州心锐医疗科技有限公司 一种医用液体输送系统
CN116990082B (zh) * 2023-06-28 2024-03-19 辽宁千一测试评价科技发展有限公司 一种基于生物体液检测用的取样装置及其取样方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2926691C2 (de) 1979-07-02 1983-05-26 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Repetierpipette
DE4341229C2 (de) * 1993-12-03 1995-09-07 Eppendorf Geraetebau Netheler Pipettensystem
DE4342178C2 (de) 1993-12-10 1995-09-28 Eppendorf Geraetebau Netheler Pipettensystem
DE4414760C1 (de) 1994-04-27 1995-08-24 Eppendorf Geraetebau Netheler Repetierpipette
DE4437716C2 (de) * 1994-10-21 1996-10-17 Eppendorf Geraetebau Netheler Repetierpipette
DE19917907C2 (de) * 1999-04-20 2001-08-16 Brand Gmbh & Co Kg Pipette für ein Pipettensystem
EP1724120B1 (fr) 2004-02-18 2013-03-06 Taica Corporation Procede de transfert hydraulique
DE102005023203B4 (de) 2005-05-20 2009-06-04 Eppendorf Ag Pipette
DE102007042115B4 (de) 2007-09-05 2010-12-02 Eppendorf Ag Pipette
DE202010010942U1 (de) * 2010-08-03 2011-11-08 Brand Gmbh + Co Kg Pipette für ein Pipettensystem mit Pipette und Spritze
EP2656916B1 (fr) 2012-04-23 2019-01-16 Eppendorf AG Procédé de dosage de liquide au moyen d'une pipette et d'une seringue ainsi que la pipette destinée à actionner une seringue pour le dosage de liquides

Also Published As

Publication number Publication date
CN103506173A (zh) 2014-01-15
JP2014036952A (ja) 2014-02-27
DE102012011938A1 (de) 2013-12-19
DE102012011938B4 (de) 2015-02-05
EP2676730A1 (fr) 2013-12-25

Similar Documents

Publication Publication Date Title
EP2676730B1 (fr) Pipette destinée à actionner une seringue
EP3164214B1 (fr) Pipette servant à faire fonctionner une seringue
EP2033712B1 (fr) Pipette
EP1828967B1 (fr) Inhalateur
DE19900792C1 (de) Vorrichtung zur Verabreichung eines injizierbaren Produkts
DE102007010299B4 (de) Handpipettiervorrichtung
DE102004003433B4 (de) Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen
EP2633915B1 (fr) Pipette avec système de calibration
DE102004003434B4 (de) Pipettiervorrichtung mit einer Verdrängungseinrichtung und einer damit lösbar verbundenen Antriebseinrichtung
EP2069094B1 (fr) Dispositif de serrage pour machine de sciage en va-et-vient
EP2165765A1 (fr) Dispositif de pipetage avec filetage double
EP2659978B1 (fr) Pipette avec systeme de blockage
EP3399214B1 (fr) Entraînement de dosage électronique
EP2783753B1 (fr) Distributeur destiné à actionner une seringue
US20140010732A1 (en) Pipette for Operating a Syringe
EP3680017B1 (fr) Pipette destinée à une utilisation avec une pointe de pipette
EP2656915A1 (fr) Pipette destinée à actionner une seringue
EP3939702A1 (fr) Pipette pour l' utilisation avec une pointe de pipette à plongeur intégré
DE4437716C2 (de) Repetierpipette
DE69116355T2 (de) Prozedur zum verriegeln der verstellvorrichtung einer pipette, und pipette
EP2415525B1 (fr) Pipette pour un système de pipetage avec pipette et seringue
EP3778028B1 (fr) Pipette à volume de dosage réglable
EP2656916B1 (fr) Procédé de dosage de liquide au moyen d'une pipette et d'une seringue ainsi que la pipette destinée à actionner une seringue pour le dosage de liquides
DE2705655A1 (de) Vorrichtung zur dosierten abgabe viskoser massen
DE10355914B3 (de) Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20131010

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

RIC1 Information provided on ipc code assigned before grant

Ipc: B01L 3/02 20060101AFI20141009BHEP

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20141121

RIN1 Information on inventor provided before grant (corrected)

Inventor name: WILMER, JENS

Inventor name: BELGARDT, HERBERT

Inventor name: REICHMUTH, BURKHARDT

Inventor name: SATTLER, JOERG

Inventor name: MOLITOR, PETER

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20150506

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

Free format text: NOT ENGLISH

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 741626

Country of ref document: AT

Kind code of ref document: T

Effective date: 20150815

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

Free format text: LANGUAGE OF EP DOCUMENT: GERMAN

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 502013000986

Country of ref document: DE

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20150812

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151113

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151112

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151212

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20151214

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 502013000986

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 4

26N No opposition filed

Effective date: 20160513

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160630

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160630

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160630

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160617

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 5

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20130617

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160617

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20150812

REG Reference to a national code

Ref country code: AT

Ref legal event code: MM01

Ref document number: 741626

Country of ref document: AT

Kind code of ref document: T

Effective date: 20180617

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20180617

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 10

REG Reference to a national code

Ref country code: DE

Ref legal event code: R081

Ref document number: 502013000986

Country of ref document: DE

Owner name: EPPENDORF SE, DE

Free format text: FORMER OWNER: EPPENDORF AG, 22339 HAMBURG, DE

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230527

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20240620

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20240619

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20240628

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20240620

Year of fee payment: 12